RU2424580C1 - Method of increasing antioxidant status of warm-blooded organism under ultraviolet irradiation - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: 20 minutes prior to an irradiation procedure in an ultraviolet chamber, an infusion tea of nettle, birch, plantain leaves is introduced orally in a laboratory animal. The infusion tea is prepared of leaves taken in the ratio 1:1:1, by keeping 8 g of the tea in boiling water 200 ml. The infusion tea is introduced at 5 ml/kg of weight daily for 28 days.

EFFECT: higher antioxidant activity and decreased degree of radical product and lipid peroxide accumulation, in the absence of side and toxic effects of the plant tea, and applicability in experimental medicine and clinical practice.

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Description

The invention relates to medicine, in particular to pharmacology, can be used to stabilize the processes of lipid peroxidation of biomembranes and increase the antioxidant status of a warm-blooded organism under ultraviolet rays and find application in experimental medicine and clinical practice.

Known methods for increasing the antioxidant status of a warm-blooded organism under the influence of prooxidant factors by the introduction of synthetic antioxidant preparations - dibunol, tocopherol acetate, mexidol [1], emoxipin [2] and a drug of the adaptogen group of plant origin eleutherococcus [3].

The disadvantages of these methods are the need for the use of pharmacological preparations of synthetic origin, having a number of side and toxic effects and a relatively high cost.

A method is proposed for stabilizing the processes of peroxidation and increasing the antioxidant status of a warm-blooded organism under ultraviolet irradiation, which is a powerful prooxidant factor, introducing an infusion based on phyto-collection from nettle, birch, and plantain leaves. The plants that make up the collection grow on the territory of our region (Amur region) and are widespread in Russia, contain a wide range of biologically active substances. The raw materials used for manufacturing are available, the production technology is cost-effective, the range of applications is wide. The infusion based on phytobar has no side and toxic effects, it has a stabilizing effect on lipid peroxidation of biomembranes, increases the activity of the antioxidant system of the body, thus exerting a multifaceted positive effect on the animal and human body under ultraviolet radiation and other prooxidant factors.

The technical result of the invention is to obtain an infusion based on phytobar from plants (nettle, birch, plantain leaves) growing in our region (Amur Region) and its use to stabilize the processes of peroxidation and increase the antioxidant status of a warm-blooded organism under ultraviolet radiation.

The method consists in the fact that experimental animals (rats or mice) under standard vivarium conditions, 20 minutes before irradiation in an ultraviolet chamber (RF Patent No. 2348079 of 02.27.2009), are infused daily with an infusion of nettle, birch leaves, plantain at the rate of 5 ml / kg weight for 28 days.

Preparation of infusion based on a collection of nettle leaves, birch, plantain. Nettle leaves (harvested during flowering), birch (harvested in May), plantain (harvested in June - July) were crushed, mixed at a ratio of 1: 1: 1, poured with boiling water at a rate of 8 g per 200 ml of water, insisted 60 minutes , filtered, the precipitate was removed, the infusion was cooled.

On the 29th day of the experiment, the animals were killed by decapitation.

The results were taken into account by the ratio of the contents of lipid peroxidation products (lipid hydroperoxides, diene conjugates, malondialdehyde) and the main components of AOS (ceruloplasmin, vitamin E) in the blood and internal organs in comparison with animals in the control group, processed by statistical methods using the Wilcoxon-Mann-Whitney criterion .

The method allows to increase the antioxidant activity and reduce the degree of accumulation of radical products and lipid peroxides in the body of UV-irradiated mice and rats without the use of pharmacological preparations of synthetic origin, having a number of side and toxic effects and relatively high cost.

The content of lipid peroxidation products (lipid hydroperoxides, diene conjugates, malondialdehyde) and the main components of AOS (ceruloplasmin, vitamin E) in the blood plasma and liver tissue of the control and experimental groups was studied (Tables 1, 2, 3, 4).

Table 1 The content of lipid peroxidation products in the blood plasma of rats subjected to ultraviolet radiation on the background of the introduction of the infusion based on the collection of nettle leaves, plantain and birch (nmol / ml) Groups of animals Lipid hydroperoxides Diene conjugates Malonic dialdehyde Control Group (Ural Federal District) 29.1 ± 1.8 31.9 ± 2.4 5.0 ± 0.3 Experimental group (UFO + infusion) 22.6 ± 1.2 (p <0.05) 20.8 ± 1.9 (p <0.05) 4,5 ± 0,5

As a result of the studies, the content of lipid hydroperoxides in the blood of animals that received an infusion on the basis of irradiation based on a collection of nettle, birch and plantain leaves is significantly lower by 32.4% than in the control (irradiated) group of animals (p <0.05) diene conjugates - by 34.8% (p <0.05), malondialdehyde - by 10%.

table 2 The content of the main components of AOS in the blood of rats subjected to ultraviolet irradiation against the background of the introduction of the infusion based on the collection of nettle, birch, and plantain leaves (μg / ml) Groups of animals Ceruloplasmin Vitamin E Control Group (Ural Federal District) 17.1 ± 1.2 82.0 ± 6.0 Experimental group (UFO + infusion) 26.0 ± 2.1 (p <0.05) 108.3 ± 6.8 (p <0.05)

In the blood of experimental animals, the content of ceruloplasmin was significantly higher by 34.3% compared with the control group of rats (p <0.05), the level of vitamin E - by 24.3% (p <0.05).

Table 3 The content of lipid peroxidation products in the liver of rats subjected to ultraviolet irradiation against the background of the introduction of the infusion based on the collection of nettle, birch, and plantain leaves (nmol / g) Groups of animals Lipid hydroperoxides Diene conjugates Malonic dialdehyde Control Group (Ural Federal District) 118.0 ± 7.2 200.9 ± 12.5 13.8 ± 1.5 Experimental group (UFO + infusion) 89.9 ± 6.8 (p <0.05) 128.6 ± 10.4 (p <0.05) 9.1 ± 0.8 (p <0.05)

In the liver tissue of the experimental group of animals, the content of lipid hydroperoxides was significantly lower by 23.9% compared with the control (p <0.05), diene conjugates - 36% (p <0.05), malondialdehyde - 34.1% (p <0.05).

Table 4 The content of the main components of AOS in the liver of rats subjected to ultraviolet irradiation against the background of the introduction of the infusion based on the collection of nettle, birch, and plantain leaves (μg / g) Groups of animals Ceruloplasmin Vitamin E Control Group (Ural Federal District) 15.9 ± 2.4 30.7 ± 2.5 Experimental group (UFO + infusion) 22.9 ± 1.1 (p <0.05) 44.4 ± 3.5 (p <0.05)

The content of ceruloplasmin in the liver tissue of the experimental group of animals was significantly higher by 30.6% compared with the control (p <0.05), the level of vitamin E - by 31% (p <0.05).

INFORMATION SOURCES

1. Mashkovsky M.D. Medicines: a manual for doctors. - M .: Medicine, 2004 - 685 p.

2. Dorovskikh V.A. Antioxidants in the prevention and correction of cold stress [Text] / V.A. Dorovskikh, E.A. Borodin, S.S. Tseluyko. - Blagoveshchensk, 2000 .-- 183 p.

3. Dorovskikh V.A. Adaptogens and cold stress: yesterday, today, tomorrow ...: monograph / V.A. Dorovskikh, N.V. Simonova, N.P. Krasavina and others. - Blagoveshchensk: DalGAU, 2006. - 214 p.

Claims (1)

A method of increasing the antioxidant status of a warm-blooded organism against the background of stabilization of lipid peroxidation processes of biomembranes under ultraviolet irradiation of laboratory animals, characterized in that experimental animals are orally infused 20 minutes before exposure to ultraviolet rays based on a collection of nettle, birch and plantain leaves taken in the ratio of 1: 1: 1, and obtained by insisting 8 g of collection per 200 ml of boiling water, while the introduction of the infusion is carried out daily at the rate of 5 ml / kg of weight for 28 days.