RU2418598C1 - Method of obtaining preparation, possessing immunomodulating, anti-inflammatory and wound-healing action (versions) - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: invention relates to chemical-pharmaceutical industry, namely, to method of obtaining preparation, which has immunomodulating, anti-inflammatory and wound-healing action, which lies in crushing preparation components dog rose fruit and/or ambrosia, drying to 10% humidity, freezing ambrozia and/or dog rose fruit separately to temperature 40-60°C with further impact on each component in jet mill by air jet under pressure 0.6-0.8 MPa until particles with size not more than 500 nm are obtained.

EFFECT: method ensures high productivity and good preservation of material properties.

2 tbl, 9 ex, 2 cl

Description

The invention relates to methods for producing a prophylactic drug - a biologically active food supplement (BAA).

The closest technical solution to the proposed method for producing a preparation having immunomodulating, anti-inflammatory and wound healing action is a method for producing a preparation consisting in mechanical fragmentation of flower pollen (see "Simple technology for processing pollen", found on the Internet http: // huashen-dp .org.ua / publ / 37-1-0-63).

Its disadvantage is low productivity and loss of properties of the source material. The walls of pollen are not completely destroyed, nutrients are lost, the assimilation and use of nutrients is limited.

The objective of the proposed method is to increase productivity and preserve the properties of the material.

For this, the components of the preparation, the rose hips and pollen are dried to a moisture content of 10%, the components are individually frozen to a temperature of 40-60 ° C, followed by exposure to each component in a jet mill with an air stream under a pressure of 0.6-0.8 MPa to obtain particles sizes not exceeding 500 nm, the resulting nanoparticles of flower pollen and nanoparticles of rose hips are mixed in the ratio, wt.%:

rosehip nanoparticles 15 ÷ 25 pollen nanoparticles  - the rest,

A distinctive feature of the proposed method is that the components of the drug rose hips and flower pollen are dried to a moisture content of 10%, the components are individually frozen to a temperature of 40-60 ° C, followed by exposure to each component in a jet mill with an air stream under a pressure of 0.6-0 , 8 MPa to obtain particles no larger than 500 nm in size, the resulting nanoparticles of flower pollen and nanoparticles of rose hips are mixed in the ratio, wt.%:

rosehip nanoparticles 15-25 pollen nanoparticles  - the rest.

Alternatively, a method for producing a preparation having an immunomodulating, anti-inflammatory and wound healing effect is proposed, which consists in crushing flower pollen, drying it to a moisture content of 10%, freezing flower pollen to a temperature of 40-60 ° C, followed by exposure to it in a jet mill under an air stream under pressure of 0.6-0.8 MPa to obtain flower pollen nanoparticles with sizes not exceeding 500 nm.

A distinctive feature of the proposed method is that the pollen is crushed, dried to a moisture content of 10%, frozen to a temperature of 40-60 ° C, followed by exposure to it in a jet mill with an air stream under a pressure of 0.6-0.8 MPa to obtain flower nanoparticles pollen with sizes not exceeding 500 nm.

The drug is obtained in the following way. Each component of the preparation, namely flower pollen and rose hips, is frozen to 10-30 ° С, freeze-drying is carried out to a moisture content of not more than 10%, then it is frozen to a temperature of 40 ÷ 60 degrees and exposed to it in a jet mill, for example, in a jet mill SM-2T Belgorod experimental mechanical plant, with an air stream under pressure of 0.6-0.8 MPa, obtained nanoparticles of flower pollen separately or together with nanoparticles of rose hips in the ratio, wt.%:

rosehip nanoparticles 15 ÷ 25 pollen nanoparticles  - the rest

hermetically sealed.

The following are the results of studies of the content of nutrients in various crushing methods.

Table number 1 Comparative tables of the content of some elements from the composition of pollen after grinding in various ways as a percentage of the original 100% Items Methods Vitamins Minerals Amino acids Enzymes Irreplaceable Interchangeable mechanical crushing 68 97 52 52 76 thermal crushing eighteen 95 0 0 0 fermentation method 35 83 45 45 65 Proposed Crushing Method 98 one hundred 97 97 97 The data in table No. 1 were obtained using methods for determining vitamins in biological objects by the well-known physicochemical method.

Table number 2 Performance comparison table for various crushing methods Methods Productivity (kg / h) mechanical crushing thirty thermal crushing twenty fermentation method 5 Proposed Crushing Method 60 When crushing by the above methods, different from the proposed, it is impossible to achieve a given degree of grinding (500 nm-800 nm).

Obtained by the proposed method, the drug is used as follows. The drug is taken on an empty stomach under the tongue in an amount of 0.3 ÷ 2 g three times a day for 20 ÷ 30 minutes before meals under the tongue for 3-4 months. At the same time, nanoscale particles penetrate the membranes of the cells of the human body, which makes the substances easily digestible (90–95%) and effective. At the same time, the immune forces of the human body increase.

Examples. Male, 47 years old. Diagnosis: prostatitis. After receiving pollen pollen, crushed to nanometers, for 3 months, 2 g three times a day 20 minutes before meals, the general state of health under the tongue improved, urination, increased potency. Improvement of blood test parameters: hemoglobin from 106 g / l to 132 g / l, lowering of white blood cells from 12.5 10 ⁹ cells / l to 6.8 10 ⁹ cells / l, ESR from 22 mm / h to 10 mm / h.

Woman, 53 years old. Frequent headaches. After receiving pollen pollen, crushed to nanometers, for 3 months, 0.3 g three times a day 20 minutes before meals, general health improved under the tongue, headaches disappeared, sleep improved, stool normalized, and constipation disappeared. Improvement of blood test parameters: hemoglobin from 92 g / l to 126 g / l, white blood cells from 10.5 10 ⁹ cells / l to 5.5 10 ⁹ cells / l, ESR from 16 mm / h to 9 mm / h.

Male, 43 years old. Diagnosis: pancreatitis. After taking a mixture of flower pollen (85%) and rose hip (15%), crushed to nanometers, 0.3 g for 1 month three times a day 20 minutes before meals, girdle pains passed under the tongue, general health improved, stool normalized . Improvement of blood test parameters: hemoglobin from 106 g / l to 132 g / l, lowering of white blood cells from 13.8 10 ⁹ cells / l to 6.0 10 ⁹ cells / l, ESR from 28 mm / h to 10 mm / h.

Girl, 15 years old. Headaches, severe pains in the lower abdomen, up to the loss of consciousness associated with the onset of menstruation. After taking a mixture of pollen (75%) and wild rose (25%), crushed to nanometers, for 1 month, 2 g three times a day 20 minutes before meals under the tongue, overall well-being improved, headaches, lower pains went away abdomen, improved sleep.

The method of application of the drug (option) is as follows.

5 g of the drug is diluted in 50 ml of water, applied to the face in the form of a mask for 20 ÷ 30 minutes, the procedure is performed once a day for a month. At the same time, nanosized particles penetrate the skin cell membranes, feeding it to the entire depth with vitamins, microelements, amino acids, flavonoids, which allows to increase the protective, regenerating and anti-aging function of the skin.

Examples

Woman, 43 years old. When using pollen pollen, crushed to nanometers, in an aqueous solution of 5 g per 50 ml of water in the form of applying a mask on the face for 20 minutes, once a day for a month I got the result: the skin of the face became elastic, silky, facial wrinkles smoothed out.

Girl, 14 years old. Diagnosis: acne on the face. The use of a mixture of pollen (75%) and wild rose (25%), crushed to nanometers, in an aqueous solution of 5 g per 50 ml of water in the form of a face mask for 20 minutes once a day for a month. Acne on the face completely passed, significantly improved psychological and physical condition.

The method of application of the drug (option) is as follows.

5 g of the drug is diluted in 20 ml of water, applied to inflamed, burn or wound areas of the body 3-8 times a day for 7-10 days. At the same time, nanosized particles penetrate the skin cell membranes, feeding it to the entire depth with vitamins, microelements, amino acids, flavanoids, which allows to increase the protective, regenerating anti-inflammatory function of the skin.

Examples

Man, 32 years old. Sunburn of hands. After applying pollen pollen, crushed to nanometers, in an aqueous solution of 5 g per 20 ml of water on the burned surface 3 times a day for 7 days, the pain sharply decreased, hyperemia and edema of the burned areas of the body went away, the skin on the hands did not peel off.

Child, 7 years old. Deep wounds on the skin of the right hand from scratching the claws of a cat.

After applying pollen pollen, crushed to nanometers, in an aqueous solution of 5 g per 20 ml of water on the wound surface, the bleeding stopped, the pains disappeared. The solution was applied 8 times a day for a week. The wounds healed without suppuration.

Woman, 53 years old. Diagnosis: fibrocystic mastopathy, condition after breast removal surgery, three courses of chemotherapy. General weakness. Persistent headaches. Long non-healing postoperative wound. The state of moderate severity. After applying a mixture of flower pollen (85%) and rosehip (15%), crushed to nanometers, in an aqueous solution of 5 g per 20 ml of water on the wound surface 6 times a day for one month, overall health improved, sleep improved, and a wound on the chest healed. Improvement of blood test parameters: hemoglobin from 92 g / l to 126 g / l, white blood cells from 13 10 ⁹ cells / l to 6.5 10 ⁹ cells / l, ESR from 32 mm / h to 11 mm / h.

Thus, a drug based on pharmacologically active substances from plant pollen and rose hips stimulates the body's defenses. Each component contains biologically active substances that simultaneously activate intracellular metabolism and metabolism, and also have a pronounced antimicrobial effect on pathogenic and conditionally pathogenic microflora.

The drug obtained by the proposed method can increase immunity, replenish the amount of vitamins, microelements, amino acids and other useful substances necessary for normal body functioning, replenish energy losses, restore the integrity of the skin during injuries and burns, reduce the dose of food additives, reduce the frequency of taking the drug .

The proposed method for the preparation of the drug ensures the preservation of the properties of the material, which allows to reduce the dose, reduce the frequency of administration, increase the effectiveness of the drug.

Claims (2)

1. A method of obtaining a drug having immunomodulatory, anti-inflammatory and wound healing effects, which consists in crushing the components of the preparation of rose hips and flower pollen, drying to a moisture content of 10%, freezing flower pollen and rose hips individually to a temperature of 40-60 ° C with subsequent exposure to each component in a jet mill with an air stream under a pressure of 0.6-0.8 MPa until particles of no more than 500 nm are obtained, while the resulting nanoparticles of flower pollen and nanoparticles of rose hips are mixed sew in the ratio, wt.%:
rosehip nanoparticles 15-25 pollen nanoparticles rest 2. A method of obtaining a drug having immunomodulatory, anti-inflammatory and wound healing effects, which consists in crushing flower pollen, drying to a moisture content of 10%, freezing flower pollen to a temperature of 40-60 ° C, followed by exposure to it in a jet mill with an air stream under pressure 0, 6-0.8 MPa to obtain particles with a size of not more than 500 nm.