RU2329794C1 - Method of animal gastrointestinal disturbance treatment - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: method includes combined introduction of antimicrobial agent that is chinoxaline derivative - "Rubolaque" in therapeutic dose and microelement agent "Helavite" in daily dose 0.2-0.4 ml/10 kg of animal live weight to recovery. Invention is effective for treatment and prevention of dyspepsia, gastroenteritis, diarrhea, colitis, dysbacteriosis, enteritis, abohmosoenteritis (of calves), dysbacteriosis, bacterial dysentery of pigs, provides normalisation of hematological, biochemical and immunological animal blood values.

EFFECT: development of effective method of gastrointestinal disturbance treatment in veterinary science.

1 ex, 10 tbl

Description

The invention relates to livestock and veterinary medicine, in particular to drugs for the treatment of gastrointestinal diseases.

Gastrointestinal diseases of animals of various etiologies are widespread in livestock and pig farms, causing them enormous economic damage resulting from mortality, forced slaughter and reduced animal productivity.

There is a wide variety of methods for treating diseases of the gastrointestinal tract in farm animals. Most treatment regimens boil down to the use of complex therapy (Antipov V.A., Shakhov A.G. Medicines for gastrointestinal diseases of piglets // Agriculture Abroad. - 1979. - No. 4. - P.49-53).

They include measures aimed at eliminating feeding technology, increasing the reactivity of the body, the use of antibacterial, antimicrobial agents, antibiotics, vitamins, trace elements and immunoglobulins. In addition to antimicrobials, in order to eliminate dysbiosis, prebiotics (lactulose) and probiotics (enterobifidil, lactobacterin, etc.) are also prescribed.

It is known the use of a broad-spectrum antibiotic - "Biovit" (Veterinary drugs in Russia: Reference book / I.F. Klenova, N.A. Yaremenko. - M .: Selkhozizdat, 2000. - P.100), used for the treatment and prevention of acute and chronic gastrointestinal and pulmonary diseases of bacterial etiology in calves, piglets, fur animals. It is used to treat pasteurellosis, colibacillosis, salmonellosis, dysentery, etc. The drug is a yellow powder, poorly soluble in water. 1 g of the drug contains 0.04-0.08 or 0.15 g of chlortetracycline hydrochloride.

Chlortetracycline hydrochloride belongs to the group of biosynthetic antimicrobial agents from the group of tetracyclines, which has a wide spectrum of action. Highly effective against gram-negative and gram-positive microorganisms, mycoplasmas, chlamydia, rickettsia, eimeria, actinomycetes. The drug is particularly effective against colibacilli and salmonella. The mechanism of action of the drug is to inhibit the synthesis of protein of microorganisms on the ribosomes, causing a bacteriostatic effect. Chlortetracycline hydrochloride penetrates well into organs, tissues and body fluids, showing a high therapeutic effect. The drug is administered orally in a mixture with food or water individually or in a group method. When using the drug in high doses, dysbiosis and the occurrence of allergic reactions are possible. It is not recommended for use with hypersensitivity to tetracyclines and long-term use in pregnant animals.

Antimicrobial preparations containing iodine as an active principle, unlike antibiotics, do not cause addiction to pathogenic microorganisms. Thus, the use of the drug "Iodinol" for the prevention and treatment of dyspepsia, dysentery, gastroenteritis, colitis and other gastrointestinal diseases of piglets, lambs, chickens is known (Veterinary preparations in Russia: Reference book / I.F.Klenova, N.A. Yaremenko. - M .: Selkhozizdat, 2000. - P.151). The complex preparation is an aqueous solution containing 0.1% iodine, 0.3% potassium iodide and 0.9% polyvinyl alcohol. It decomposes in the light and in an alkaline environment. Iodinol has a wide antimicrobial spectrum of activity in relation to gram-positive and gram-negative microflora. With gastrointestinal diseases, the drug is administered orally. The calves are given the drug daily until complete recovery 2 times a day at a dose of 1-1.5 ml per 1 kg of animal weight. Piglets-suckers - in a dose of 5-10 ml per piglet 2 times a day until recovery. Contraindication - hypersensitivity to iodine preparations.

It is also known the use of the drug "Karbolak" for the treatment of young farm animals with bacterial etiology of gastroenteritis caused by pathogens sensitive to the components of the drug (E. coli, Salm.cholerasuis), including piglet dysentery caused by Treponema hyodysenteria. The drug is also used for diseases of the respiratory tract (pneumonia, bronchopneumonia), including piglet bordetelliosis. The drug is prescribed orally once or twice a day until recovery in a dose of 0.1-0.15 ml / kg of body weight, depending on the condition of the sick animal.

Suspensio “Carbolacum” suspension is a slightly exfoliating, opaque yellowish-greenish liquid with a slightly specific odor (instructions for the use of “Karbolak” suspension in veterinary medicine). In 1.0 ml of the drug contains 0.045 g of olakvindoksa, 0.02 g of carbadox, excipients and fillers up to 1.0 ml. Olakvindoks and carbadox are synthetic antimicrobials derived from quinoxaline. Olakvindoks has a bactericidal effect mainly against gram-negative bacteria, and carbadox mainly against gram-positive. Olakvindoks is poorly absorbed from the gastrointestinal tract and creates a therapeutic concentration in the lumen of the intestine. Carbadox is well absorbed from the digestive tract, penetrates into organs and tissues, and accumulates mainly in the lung tissue.

The mechanism of action of the drug is to disrupt protein synthesis at the DNA level, it reduces the permeability of the cell membranes of microorganisms to divalent metal ions, while violating the ionic equilibrium in the microbial cell, which leads to the death of the microorganism. When using the drug, an increase in diuresis is possible due to blockade of the antidiuretic hormone, thirst.

Known trace element preparation for animals - "Chelavit" (RU 2227800 C1, publ. 06/20/2006 Bull. No. 17, A23K 1/175, A61K 31/295), consisting of the following components, wt.%: 2Na- or 2K -Salt of ethylenediamine-N, N ¹ -disuccinic acid 15.0-35.0; Na- or K-salt of amino acids 2.0-10.0; iron (III) 0.6-3.0; manganese (II) 0.5-2.5; copper (II) 0.05-0.25; zinc (II) 0.3-2.5; cobalt (II) 0.005-0.05; selenium (IV) 0.01-0.03; iodine (I) 0.03-0.08; water the rest. In this preparation, trace elements are in a balanced state in the form of chelates. The use of the drug enhances the reproductive ability of animals, stimulates the growth, development and productivity of agricultural and domestic animals. However, the use of the drug for the treatment and prevention of gastrointestinal diseases in animals is unknown.

It is known to use quinoxaline-olaquindox derivative in a dose of 0.005-0.01 g / kg 1-2 times a day for the treatment and prevention of gastroenteritis of piglets and young animals of other animal species. Olakvindoks is part of the drug suspension "Rubolak". Olakvindoks (2- (N-2-hydroxymethylcarbamyl) -3-methylquinoxaline-1,4-dioxide) - a derivative of quinoxaline, has an antimicrobial effect. A polyacrylic acid gel was used as the basis for the preparation, which significantly improves the pharmacokinetic features and stability of the suspension. The active substance and the drug itself is highly effective in the prevention and treatment of young farm animals with pathology of the gastrointestinal tract (B.Ya. Birman, Ya.E. Kinegsberg, A.T. Ivanov, N.V. Zakharik, I.V. Nasonov, T.G. Nyattieva, Prophylactic efficacy of olaquindox tritiurate in gastrointestinal, respiratory diseases of calves // Veterinary production science: Interdepartmental collection of Bel.NIIEV named after Vyshelevsky, Issue 29, Minsk .: Urajay. - 1991. - P. 45 ); Veterinary Medicines in Russia: A Handbook in 2 Volumes. T.I. / I.F.Klenova, K.L. Maltsev, N.A. Yaremenko, I.A. Arkhipov. - M .: Selkhozizdat, 2004. - P.236).

However, in some cases, the drug is not effective enough due to various reasons that cause gastrointestinal disorders, in which the immunobiochemical homeostasis of animals worsens, in particular, cellular and humoral immunity and natural resistance.

The objective of the invention is to increase the effectiveness of the method of treatment of gastrointestinal diseases in animals.

To solve this problem, a method of treating gastrointestinal diseases in animals, comprising oral administration of an antimicrobial drug, quinoxaline derivative “Rubolac” in a therapeutic dose, according to the invention, the microelement preparation “Helavit” is additionally administered to animals in a daily dose of 0.2-0, 4 ml / 10 kg of live weight of the animal until recovery.

In the claimed method, it is the use of complex therapy, including the simultaneous administration of an antimicrobial preparation and a microelement preparation containing biologically active microelements in the form of chelates, which has significantly increased the effectiveness of treatment and, accordingly, reduced the treatment time of animals.

The drug "Helavit" contains in its composition, g / l: iron - 20.0; Manganese - 14.0; copper - 2.0; zinc - 18.0; cobalt - 0.24; selenium - 0.22; iodine - 0.52.

The drug "Rubolak" contains an active substance, a quinoxaline derivative - olaquindox - 2- (N-2-hydroxymethylcarbamyl) -3-methylquinoxaline-1,4-dioxide, which has an antimicrobial effect. Olakvindoks is used in a dose of 0.005-0.001 g / kg 1-2 times a day. The drug "Rubolak" based on olakvindoksa as a base contains a gel of polyacrylic acid, which significantly improves the pharmacokinetic characteristics and stability of the suspension.

In studies, Rubolak was used in a therapeutic dose of 1 ml / 4.5 kg of animal weight once a day. In the inventive method, the drug "Helavit" was used in a daily dose of 0.2-0.4 ml per 10 kg of live weight. When using a dose of less than 0.2 ml / 10 kg of live weight, efficiency decreases, and when a dose of more than 0.4 ml / 10 kg of live weight is introduced, the cure time of the animals did not significantly increase.

Both drugs are introduced to sick animals in food, in addition, “Helavit” can be introduced with water due to its good solubility. The drugs are well combined with each other.

The effectiveness of the application of the claimed method can be illustrated by the following examples.

Example 1. According to the analogue method, large white piglets of 45-50 days old were selected in an amount of 10 animals each with clinical signs of gastroenteritis. The following groups were formed:

The first group received with the drug “Helavit” in a dose of 0.2 ml / 10 kg of live weight once a day.

2nd group - “Chelavit” at a dose of 0.2 ml / 10 kg and “Rubolak” at a dose of 1 ml / 4.5 kg of the animal’s weight once a day.

3rd group — a single injection of ferroglukin-75 at a dose of 3 ml per head was performed.

4th group - received complex therapy - “Ferroglukin” and “Rubolak” in similar doses.

5th group - received "Rubolak" 1 time per day at a dose of 1 ml / 4.5 kg of animal weight.

6th group - control (treatment measures were not performed).

Blood was taken from experimental animals before treatment, after one day, on the fourth, seventh, tenth day. We analyzed biochemical, clinical, immunological parameters. The appearance of the animals and the weight gain of the piglets were also determined with various treatment regimens. The data obtained are shown in tables 1-7.

The results of the analysis of the duration of the manifestation of clinical symptoms of gastroenteritis in piglets with various treatment regimens are given in table 1.

Table 1
The duration of the manifestation of clinical symptoms of gastroenteritis in piglets with various treatment regimens, days (M ± m) Groups Oppression or perversion of appetite Skin elasticity reduction Eyeball retraction and conjunctival anemia Diarrhea (diarrhea) one 3.9 ± 0.3 * 4.7 ± 0.4 * 4.2 ± 0.4 * 5.9 ± 0.15 2 1.2 ± 0.3 * 2.6 ± 0.3 * 2.7 ± 0.3 * 3.1 ± 0.08 * 3 4.9 ± 0.4 * 5.6 ± 0.5 * 5.3 ± 0.4 * 8.3 ± 0.16 four 2.1 ± 0.5 * 3.1 ± 0.8 * 3.3 ± 0.6 * 3.6 ± 0.12 5 2.0 ± 0.3 * 3.3 ± 0.4 * 3.2 ± 0.3 * 3.9 ± 0.15 * 6 7.2 ± 1.90 9.1 ± 2.20 8.0 ± 1.8 10.0 ± 1.16 Hereinafter: The difference compared with the control (group No. 6) is significant at * p <0.05; ** p <0.01; *** p <0.001.

As follows from the data of table 1, the claimed method of treatment allowed to reduce the cure time to 3.1 days compared with the control (10.0 days). In untreated animals, disease symptoms were observed within 10 days.

The methods with the introduction of rubolac (3.9 days) and the combined administration of ferroglukin and rubolac (3.6 days) were quite effective.

The therapeutic effectiveness of therapeutic measures in the first group was 67.1%, the third - 62.4%, the fourth - 62.9%, the fifth - 70.9%, while the second (the claimed method) - 88.3%.

The hematological parameters of patients with gastroenteritis of piglets with basic treatment regimens and with the introduction of chelavitis are presented in Tables 2 and 3.

In the second group, receiving rubolac and helavit, on the 10th day the hemoglobin level increased to 100.5 g / l, red blood cells - up to 6.25 × 10 ¹² / l; in the control, respectively, 93.1 g / l and 5.76 × 10 ¹² / l.

In piglets treated with rubolac and helavit, the number of leukocytes also decreased to 18.12 × 10 ⁹ / l (in the control - 24.38 × 10 ⁹ / l).

table 2
Hematological parameters of patients with gastroenteritis of piglets with basic treatment regimens (M ± m) Indicators Groups of animals Duration of observation from the start of treatment before treatment in one day on the fourth day on the seventh day on the tenth day Hemoglobin, g / l four 94.4 ± 0.86 95.4 ± 0.86 97.1 ± 0.50 *** 98.4 ± 0.75 *** 99.7 ± 0.58 *** 5 94.6 ± 0.96 95.2 ± 0.70 96.4 ± 0.70 * 96.7 ± 0.70 ** 97.2 ± 0.78 *** 6 94.1 ± 0.74 93.7 ± 0.56 94.1 ± 0.53 93.9 ± 0.62 93.1 ± 0.71 Hematocrit, l / l four 0.48 ± 0.010 0.47 ± 0.011 0.44 ± 0.012 0.43 ± 0.011 ** 0.42 ± 0.011 ** 5 0.47 ± 0.013 0.46 ± 0.009 0.45 ± 0.011 0.44 ± 0.011 * 0.43 ± 0.010 ** 6 0.47 ± 0.011 0.47 ± 0.011 0.47 ± 0.011 0.47 ± 0.011 0.48 ± 0.013 Red blood cells, 10 ¹² / L four 5.94 ± 0.307 5.98 ± 0.334 6.00 ± 0.344 6.01 ± 0.360 6.03 ± 0.352 5 5.88 ± 0.387 5.92 ± 0.396 5.57 ± 0.400 5.95 ± 0.405 5.89 ± 0.416 6 5.89 ± 0.340 5.87 ± 0.351 5.85 ± 0.324 5.82 ± 0.365 5.76 ± 0.363 White blood cells, 10 ⁹ / L four 23.97 ± 0.226 22.56 ± 0.247 *** 21.65 ± 0.347 *** 20.26 ± 0.304 *** 19.43 ± 0.181 *** 5 23.86 ± 0.287 21.87 ± 0.335 *** 19.78 ± 0.256 *** 18.55 ± 0.146 *** 18.34 ± 0.100 *** 6 23.98 ± 0.417 24.48 ± 0.270 24.50 ± 0.328 24.30 ± 0.032 24.38 ± 0.232 ESR, mm / hour four 6.7 ± 0.65 5.5 ± 0.72 5.1 ± 0.66 4.2 ± 0.77 * 3.6 ± 0.37 *** 5 6.3 ± 0.65 5.2 ± 0.68 4.7 ± 0.58 * 4.3 ± 0.45 *** 3.6 ± 0.50 *** 6 6.3 ± 0.47 6.1 ± 0.50 6.1 ± 0.41 6.6 ± 0.40 6.8 ± 0.49

Table 3
Hematological parameters of patients with gastroenteritis of piglets during treatment using the chelavit drug (M ± m) Indicators Groups of animals Duration of observation from the start of treatment before treatment in one day on the fourth day on the seventh day on the tenth day Hemoglobin g / l one 94.0 ± 0.58 95.3 ± 0.56 96.8 ± 0.84 * 97.2 ± 0.92 ** 97.3 ± 1.19 ** 2 94.2 ± 0.88 96.3 ± 0.62 ** 99.0 ± 0.88 *** 99.9 ± 0.60 *** 100.5 ± 0.58 *** 3 94.2 ± 0.84 97.8 ± 0.66 ** 96.8 ± 0.70 * 95.9 ± 0.91 95.8 ± 0.87 * 6 94.1 ± 0.74 93.7 ± 0.56 94.1 ± 0.53 93.9 ± 0.62 93.1 ± 0.71 Hematocrit l / l one 0.45 ± 0.013 0.45 ± 0.119 0.44 ± 0.011 * 0.43 ± 0.012 ** 0.41 ± 0.011 ** 2 0.47 ± 0.012 0.45 ± 0.013 0.43 ± 0.018 * 0.41 ± 0.014 ** 0.40 ± 0.012 *** 3 0.48 ± 0.015 0.46 ± 0.010 0.44 ± 0.011 * 0.45 ± 0.012 0.42 ± 0.013 ** 6 0.47 ± 0.011 0.47 ± 0.011 0.47 ± 0.011 0.47 ± 0.011 0.48 ± 0.013 Red blood cells 10 ¹² / l one 5.88 ± 0.286 5.91 ± 0.278 6.03 ± 0.293 5.97 ± 0.280 6.03 ± 0.289 2 5.92 ± 0.228 5.95 ± 0.242 6.12 ± 0.259 6.23 ± 0.243 6.25 ± 0.246 3 5.92 ± 0.349 5.93 ± 0.366 5.95 ± 0.355 5.96 ± 0.359 5.89 ± 0.352 6 5.89 ± 0.340 5.87 ± 0.351 5.85 ± 0.324 5.82 ± 0.365 5.76 ± 0.363 White blood cells 10 ⁹ / l one 23.77 ± 0.211 22.11 ± 0.233 * 22.13 ± 0.311 ** 21.43 ± 0.341 ** 21.72 ± 0.229 ** 2 23.91 ± 0.229 21.97 ± 0.387 *** 20.39 ± 0.477 *** 19.00 ± 0.278 *** 18.12 ± 0.104 ** 3 23.87 ± 0.295 22.06 ± 0.278 * 22.23 ± 0.286 *** 22.10 ± 0.272 * 22.47 ± 0.272 * 6 23.98 ± 0.417 24.48 ± 0.270 24.50 ± 0.328 24.30 ± 0.032 24.38 ± 0.232 ESR mm / hour one 5.9 ± 0.60 5.3 ± 0.79 5.2 ± 0.79 5.1 ± 0.64 * 4.9 ± 0.30 * 2 6.4 ± 0.48 5.4 ± 0.54 4.1 ± 0.28 *** 3.3 ± 0.50 *** 2.1 ± 0.28 *** 3 6.9 ± 0.71 6.8 ± 0.59 6.7 ± 0.51 6.3 ± 0.32 6.1 ± 0.63 6 6.3 ± 0.47 6.1 ± 0.50 6.1 ± 0.41 6.6 ± 0.40 6.8 ± 0.49

The data in Tables 2 and 3 indicate normalization of the metabolism of sick animals, the proposed treatment regimen allowed to stimulate hematopoiesis.

Immunological parameters for various treatment regimens for sick piglets are given in tables 4 and 5.

The second group showed a sharp increase in the bactericidal activity of blood serum (BASK) by 22.6% and phagocytic activity (FA) by 54.4% in relation to the control. Compared to the control, the phagocytic number (PS) and the phagocytic index (PI) increased significantly by 43.2% and 55.5%, respectively.

The claimed method is more effective than other treatment regimens. The data in Tables 4 and 5 indicate an increase in the natural resistance of animals when using the proposed method, which reduces the cure time.

Table 4
Indices of natural resistance of piglets suffering from gastroenteritis, with basic treatment regimens (M ± m) Indicators Groups of animals Duration of observation from the start of treatment before treatment in one day on the fourth day on the seventh day on the tenth day BASK,% four 39.89 ± 1.685 40.32 ± 1.773 40.52 ± 1.713 41.37 ± 1.747 41.46 ± 1.679 5 39.95 ± 2.161 40.48 ± 1.478 42.00 ± 2.050 41.24 ± 1.531 41.26 ± 1.520 6 39.15 ± 1.931 39.02 ± 1.771 38.68 ± 1.907 38.46 ± 1.956 38.35 ± 1.943 FA,% four 19.7 ± 0.72 20.0 ± 0.74 20.9 ± 0.74 21.2 ± 0.97 21.4 ± 0.97 5 19.3 ± 0.82 19.9 ± 0.72 20.3 ± 0.68 20.0 ± 0.71 20.2 ± 0.61 6 19.6 ± 0.72 19.4 ± 0.90 19.4 ± 0.99 19.2 ± 0.90 19.3 ± 0.76 Ff four 6.17 ± 0.441 6.43 ± 0.401 6.53 ± 0.399 6.49 ± 0.390 7.07 ± 0.381 * 5 6.27 ± 0.458 6.41 ± 0.474 6.51 ± 0.423 6.32 ± 0.400 6.53 ± 0.429 6 6.03 ± 0.432 5.83 ± 0.372 5.99 ± 0.389 5.88 ± 0.466 5.84 ± 0.376 FI four 5.25 ± 0.206 5.53 ± 0.198 5.83 ± 0.115 ** 6.01 ± 0.138 *** 6.12 ± 0.169 *** 5 5.56 ± 0.245 5.43 ± 0.233 5.35 ± 0.147 5.34 ± 0.197 5.34 ± 0.124 6 5.18 ± 0.227 4.84 ± 0.296 4.99 ± 0.212 4.90 ± 0.193 4.81 ± 0.248

Table 5
Indices of natural resistance of piglets suffering from gastroenteritis in the treatment using helavitis (M ± m) Indicators Groups of animals Duration of observation from the start of treatment before treatment in one day on the fourth day on the seventh day on the tenth day BASK,% one 39.57 ± 1.501 40.29 ± 1.664 42.67 ± 1.835 44.25 ± 1.899 * 45.44 ± 2.190 * 2 39.3 ± 1.666 42.74 ± 1.960 45.52 ± 1.865 * 48.17 ± 1.891 ** 47.03 ± 1.983 ** 3 39.67 ± 1.479 40.29 ± 1.664 42.67 ± 1.835 41.25 ± 1.899 41.84 ± 1.87 6 39.15 ± 1.931 39.02 ± 1.771 38.68 ± 1.907 38.46 ± 1.956 38.35 ± 1.943 F,% one 19.3 ± 0.84 20.2 ± 0.71 21.5 ± 0.81 23.0 ± 1.00 ** 24.4 ± 0.75 *** 2 20.0 ± 0.89 21.9 ± 0.94 24.9 ± 0.91 *** 27.6 ± 0.78 *** 29.8 ± 0.87 *** 3 19.7 ± 0.83 19.9 ± 0.87 21.0 ± 0.68 22.0 ± 0.67 * 22.1 ± 0.67 ** 6 19.6 ± 0.72 19.4 ± 0.90 19.4 ± 0.99 19.2 ± 0.90 19.3 ± 0.76 Ff one 6.40 ± 0.403 6.75 ± 0.438 7.19 ± 0.349 * 7.23 ± 0.43 * 7.49 ± 0.42 ** 2 6.37 ± 0.359 6.89 ± 0.330 7.37 ± 0.344 * 7.89 ± 0.319 ** 8.36 ± 0.342 *** 3 6.01 ± 0.419 6.22 ± 0.383 6.59 ± 0.382 7.00 ± 0.399 ** 7.46 ± 0.439 ** 6 6.03 ± 0.432 5.83 ± 0.372 5.99 ± 0.389 5.88 ± 0.466 5.84 ± 0.376 FI one 5.20 ± 0.180 5.97 ± 0.239 * 5.74 ± 0.218 * 6.13 ± 0.243 *** 6.55 ± 0.185 *** 2 5.33 ± 0.154 6.11 ± 0.156 ** 6.64 ± 0.180 *** 6.96 ± 0.200 *** 7.48 ± 0.153 ** 3 5.10 ± 0.140 5.22 ± 0.095 5.51 ± 0.117 ** 5.95 ± 0.088 *** 6.34 ± 0.160 *** 6 5.18 ± 0.227 4.84 ± 0.296 4.99 ± 0.212 4.90 ± 0.193 4.81 ± 0.248

The data on the body weight gain of piglets suffering from gastroenteritis with various treatment regimens are given in Tables 6 and 7.

By reducing the treatment time, the proposed method provided an average daily increase of 258.0 g, while in the control it was 90.0 g. The treatment method with the introduction of only the antimicrobial drug rubolac provided an increase of 205.1 g, a method for the combined treatment of rubolac and ferroglukin 222 g. The data in Tables 6 and 7 also indicate the effectiveness of the proposed method.

Table 6
Weight gain of piglets with the basic treatment regimens for piglets with gastroenteritis (M ± m) Indicators Group 6 5 four Before experience, kg 6.1 ± 0.19 6.2 ± 0.13 6.1 ± 0.11 The end of the experiment, kg 7.0 ± 0.07 7.0 ± 0.09 ** 6.9 ± 0.09 The duration of the disease, days. 10.0 ± 0.10 3.9 ± 0.15 ** 3.6 ± 0.12 ** The average daily weight gain, g 90.0 ± 2.17 205.1 ± 1.67 ** 222.2 ± 1.54 ***

Table 7
Weight gain for piglets in the treatment of piglets with gastroenteritis using the Helavit preparation (M ± m) Indicators Group 6 3 one 2 Before experience, kg 6.1 ± 0.11 6.2 ± 0.14 6.1 ± 0.10 6.1 ± 0.19 The end of the experiment, kg 7.0 ± 0.07 7.3 ± 0.09 7.2 ± 0.10 6.9 ± 0.14 ** The duration of the disease, days. 10.0 ± 0.10 8.3 ± 0.16 ** 5.9 ± 0.15 ** 3.1 ± 0.08 *** The average daily weight gain, g 90.0 ± 2.17 132.5 ± 1.70 *** 186.4 ± 0.70 *** 258.0 ± 1.54 ***

Histological examinations of the liver, kidneys, intestines, spleen, mesenteric lymph nodes were also performed with selective diagnostic slaughter of piglets during treatment and during recovery.

The results of histological studies correlate with the above dynamics of biochemical parameters. So in animals of the control group in the mucous membrane of the small intestine were found signs of catarrhal inflammation with phenomena of edema of the connective tissue base, mucus hypersecretion with goblet enterocytes of crypts, desquamation of the glandular epithelium, moderate leukocyte infiltration of the connective tissue of the lamina propria. Some reactive changes were recorded in the mesenteric lymph nodes and red spleen pulp.

The most significant morphological changes were found in the parenchyma of the kidneys and especially the liver. In addition to the phenomena associated with the reaction of the vascular system and a violation of the water balance between blood and tissue fluid, which was manifested in a significant severity of edematous processes in the stroma, signs of granular and small-droplet hydropic dystrophy were revealed in the renal tubule epithelial cells. Even more vivid signs of dystrophic changes were noted in the hepatocytes of the hepatic beams. The nature of the discompletion of the structural components of the cytoplasm of cells also indicates the presence of severe hydropic dystrophy. This is confirmed by changes in the nuclei of cells. In many hepatocytes, they are irregular in shape, pycnocental.

In animals of all experimental groups, processes of intensive regeneration in the above organs and tissues were observed. However, these processes occurred most intensively in piglets, which included the helavit drug at a dose of 0.2 ml / 10 kg of animal weight in the treatment regimen, which indicates the recovery function of the drug. It should be noted that complete restitution during these periods in the piglets of all experimental groups did not occur.

As a result of the studies, it was found that the meat of pigs to which the claimed method was used for therapeutic purposes, in terms of organoleptic, physico-chemical parameters, biological value and harmlessness is not inferior to the meat of healthy animals. Thus, the use of the method does not adversely affect the quality of pork.

Example 2. Determining the effectiveness of the claimed method for the treatment of dyspepsia in calves was performed on calves aged 8-12 days, selected by the method of analogues with clinical signs of dyspepsia in an amount of 10 goals. The treatment regimens were as follows:

The first group received with the drug “Helavit” in a dose of 0.4 ml / 10 kg of live weight once a day.

2nd group - “Chelavit” at a dose of 0.4 ml / 10 kg and “Rubolak” at a dose of 1 ml / 4.5 kg of animal weight once a day.

3rd group — a single injection of ferroglukin-75 at a dose of 3 ml per head was performed.

4th group - received complex therapy - “Ferroglukin” and “Rubolak” in similar doses.

5th group - received "Rubolak" 1 time per day at a dose of 1 ml / 4.5 kg of animal weight.

6th group - control (treatment measures were not performed).

In experimental and control animals, the duration of the manifestation of clinical symptoms of dyspepsia and the dynamics of the increase in body weight of animals were recorded. The data obtained are given in table 8-10.

Table 8
The duration of the manifestation of clinical symptoms of dyspepsia in calves with various treatment regimens, days (M ± m). Groups Appetite suppression Skin elasticity reduction Eyeball retraction and conjunctival anemia Diarrhea (diarrhea) one 2.9 ± 0.3 4.2 ± 0.4 4,5 ± 0,6 5.3 ± 0.11 2 1.1 ± 0.2 2.3 ± 0.4 2.4 ± 0.3 2.9 ± 0.1 3 4.4 ± 0.4 5.1 ± 0.5 4.9 ± 0.6 7.9 ± 0.16 four 2.1 ± 0.3 2.8 ± 0.7 2.9 ± 0.5 3.4 ± 0.18 5 2.1 ± 0.3 3.1 ± 0.6 3.0 ± 0.4 3.5 ± 0.11 6 6.9 ± 1.31 8.7 ± 2.40 8.5 ± 1.8 9.5 ± 1.22

As follows from the data of Tables 8-10, the proposed method of treatment provided calves recovery 2.9 days after the start of the therapeutic course, while in the control the clinical signs of dyspepsia were observed up to 9.5 days. The treatment according to the proposed method also provides the maximum increase in calf body weight in excess of the basic treatment regimens (tables 9, 10).

Table 9
The increase in body weight (g) of calves with the basic treatment regimens for calves with dyspepsia (M ± m). Indicators Calf group / indicators 6 5 four Before experience, kg 32.6 ± 2.85 33.1 ± 2.43 32.5 ± 2.11 The end of the experiment, kg 34.4 ± 2.17 34.2 ± 1.99 33.6 ± 1.51 The duration of the disease, days. 9.5 ± 1.22 3.5 ± 0.11 3.4 ± 0.18 The average daily weight gain, g 192.0 ± 2.17 308.9 ± 1.67 329.2 ± 1.54

Table 10
The increase in body weight (g) in the treatment of calves with dyspepsia using the drug chelavit (M ± m). Indicators Calf group / indicators 6 3 one 2 Before experience, kg 32.6 ± 2.85 31.6 ± 1.99 32.2 ± 2.11 32.6 ± 2.12 The end of the experiment, kg 34.4 ± 2.17 33.5 ± 1.90 33.6 ± 1.60 33.6 ± 2.14 The duration of the disease, days. 9.5 ± 1.22 7.9 ± 0.16 5.3 ± 0.11 2.9 ± 0.1 The average daily weight gain, g 192.0 ± 2.17 238.5 ± 1.70 259.5 ± 0.70 362.0 ± 1.54

Thus, the proposed method provides the effectiveness of the treatment of animals with gastrointestinal diseases and normalizes the hematological, biochemical and immunological parameters of the blood of animals. The method can be used to treat dyspepsia, gastroenteritis, diarrhea, colitis, dysbiosis, enteritis, abomozoenteritis (in calves), dysbiosis, bacterial dysentery in piglets.

Claims (1)

A method of treating gastrointestinal diseases in animals, comprising the oral administration of an antimicrobial drug, quinoxaline derivative “Rubolac” in a therapeutic dose, characterized in that the animal is additionally injected with a microelement preparation “Helavit” in a daily dose of 0.2-0.4 ml / 10 kg of live weight of the animal until recovery.