RU2313353C2 - Method of controlled hyperthermia - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: it is necessary to fulfill total body heating at preliminary intramuscular injection of a substance that increases the resistance to hyperthermia. The substance mentioned is represented by zootoxin. The innovation provides increased body resistance to hyperthermia and body survival rate.

EFFECT: higher efficiency of controlled hyperthermia.

4 cl, 1 tbl

Description

The invention relates to experimental medicine and can be used in oncology.

A known method of hyperthermia of an organism in an experiment (USSR author's certificate No. 1833728, IPC A61F 7/00, publ. 1993), which includes artificial ventilation of the lungs with heated perfluorodecalin.

The disadvantages of this method include: the complexity of the experiment, the use of drugs and muscle relaxants, intubation of the respiratory tract.

There is also known a method of general controlled hyperthermia of the human body according to the patent of the Russian Federation No. 21266667, IPC A61F 7/00, A61K 31/395, publ. 1999, including general warming up of the body by immersion in hot water, using fentanyl anesthesia, artificial ventilation of the lungs and administration of urotropin before, at the height of heating and after restoration of normothermia.

This method has the same disadvantages as the hyperthermia method according to as No. 1833728.

Known methods of controlled hyperthermia, including general heating of the body with preliminary intramuscular administration of a substance that increases resistance to hyperthermia (see, for example, descriptions for inventions by the USSR AS No. 1487415, 1498021, 1547279, 1552605, 1591427 and RF patent No. 2210370 ) Derivatives of heterocyclic substances, in particular hydrazide, are used as a substance that increases resistance to hyperthermia.

Of this series of known methods, the closest in general technical essence and the maximum achieved result is the method disclosed in the description of the USSR copyright certificate No. 1547279, IPC C07D 487/04, publ. 1996).

The known method, selected as the closest analogue, involves heating the body with preliminary intramuscular injection of a substance that increases resistance to hyperthermia. 8- (2-dimethylaminoethylthio) -8-methyl-1,2,4-triazino [5,6-b] indole dihydrochloride was used as the indicated substance.

The data presented indicate that when using this compound, 85% of the animals survive compared to 20% of the animals of the control group receiving saline.

The disadvantage of this method is the relatively low percentage of animal survival in hyperthermia.

The technical result of the claimed invention is to increase the body's resistance to hyperthermia.

The specified technical result is achieved by the fact that in the method of controlled hyperthermia, which includes general heating of the body with preliminary intramuscular administration of a substance that increases resistance to hyperthermia, according to the invention, zootoxin is used as a substance that increases resistance to hyperthermia. In addition, they use mucus venom at a dose of 4 mg / kg. Efa venom at a dose of 3 mg / kg can also be used. It is possible to use cobra venom at a dose of 1 mg / kg. Bee venom in a dose of 5 mg / kg can be used. The invention characterized by the above essential features at the filing date of the application is not known in the Russian Federation and abroad and meets the requirements of the criterion of "novelty."

The invention can be implemented industrially using known technical means and technologies, which allows us to conclude that it meets the requirements of the criterion of "industrial applicability".

The use of zootoxins in medicine is known, for example, for the manufacture of various therapeutic ointments, however, at the filing date of the application, its effect on the body's resistance to hyperthermia is unknown, and therefore it can be concluded that the patentability condition is “inventive step”.

An example of a specific implementation of the method.

The conditions of an overheating microclimate (50 ° С, 30% humidity) were created in a climatic chamber. The studies were performed on 150 laboratory non-linear male rats weighing 200 ± 10 g. The introduction of zootoxins was carried out intraperitoneally 10 minutes before heating at the doses indicated in the table. Control animals received saline in the same volume.

Table The effect of zootoxins and the prototype drug (1) on the survival of animals in hyperthermia Test drug Surviving animals Start of experiment End of experiment n % n % 1 (prototype) thirty one hundred fourteen 47 Thyroid Mud Poison (4 mg / kg) thirty one hundred thirty one hundred Efa venom (3 mg / kg) thirty one hundred thirty one hundred Cobra venom (1 mg / kg) thirty one hundred thirty one hundred Bee venom (5 mg / kg) thirty one hundred thirty one hundred Saline thirty one hundred 0 0 Note: Survival assessment was performed after 100% death of control animals.

The experimental data obtained indicate the high efficiency of the proposed method. The use of zootoxins as substances that increase the body's resistance to hyperthermia makes it possible to obtain a thermoprotective effect that is approximately twice as effective as the prototype compound according to the USSR copyright certificate No. 1547279.

Claims (5)

1. A method of controlled hyperthermia, including general warming of the body with preliminary intramuscular administration of a substance that increases resistance to hyperthermia, characterized in that zootoxin is used as a substance that increases resistance to hyperthermia. 2. The method according to claim 1, characterized in that they use the poison of the muzzle in a dose of 4 mg / kg 3. The method according to claim 1, characterized in that efa poison is used in a dose of 3 mg / kg. 4. The method according to claim 1, characterized in that the cobra venom is used in a dose of 1 mg / kg. 5. The method according to claim 1, characterized in that they use bee venom in a dose of 5 mg / kg