RU2208630C1 - Method for preparing fodder protein product - Google Patents

Abstract

FIELD: biotechnology, microbiology. SUBSTANCE: method for preparing fodder protein product involves culturing yeast in tricomponent nutrient medium consisting of post-alcohol vinasse (hydrolysis of grain) where bran is added. Prepared mixture is subjected for heat treatment at 70-90 C and then after its cooling up to 30-35 C alcohol condensate comprising 20% of ethyl alcohol is added. In the continuous culturing process the output is 6.9-7.6 kg/m³/h at content of crude protein 49.7-50% in ready product. Method ensures to enhance productivity of working alcohol plants, to improved quality of ready product by the content of crude protein. EFFECT: improved preparing method, increased yield of product. 5 cl, 2 tbl, 2 ex

Description

 The invention relates to the field of biotechnology, and in particular to a method for producing fodder biomass, can be used in the field of microbiological industry.

A known method for producing biomass of microorganisms (Patent RU, 2103349), in which starch-containing raw materials (substandard grain and waste from flour milling) is subjected to enzymatic hydrolysis using a multi-enzyme composition (cellloviridin GZx, amylorizin, P10x or amylosubtilin GZx or G10x); with weak heating to 50-70 ^o C, pH 4.5-6.5 for 20-160 min, using yeast Endomycopsis fibuligera VKPM - Y2173 or Aspergillus niger VKPM F-710 fungus producing amylolytic and hydrolytic enzymes.

 The method complicated by the preparation of a nutrient medium, which uses expensive amylolytic, cellulolytic and proteolytic enzymes.

As a prototype, patent RF 2159287 "A method for producing a protein feed additive" was adopted, in which yeast and bacteria with amylolytic activity are grown on a mixture of post-alcohol stillage grains with starch-containing raw materials (bran, flour), subjected to preliminary heat treatment for 1 hour at 60- 100 ^o C and pH 4.0-5.5.

When grown in the apparatus of intensive mass transfer on a two-component nutrient medium (grain stillage + 5% bran), productivity was 5.9-6.1 kg / m ³ / h and the crude protein content in the finished product was 43-44%.

The increase in productivity (6.8-7.2 kg / m ³ / h) was achieved by adding to the bard a 5% mixture of bran and flour (1: 1).

The highest productivity (8.7-9.1 kg / m ³ / h) with a crude protein content of 46.0-46.8% was obtained when growing a culture on bard with 5% flour.

 However, the use of flour as an additive to post-alcohol distillery stillage is impractical, since flour is an expensive raw material and has an independent purpose.

The essence of the invention lies in the fact that the protein feed product is obtained by culturing yeast in a nutrient medium consisting of post-alcohol stillage vinasse (hydrolysis or grain), into which bran is introduced; the resulting mixture is subjected to heat treatment at 70-90 ^o C, and then after cooling to 30-35 ^o C add alcohol condensate containing 20% ethyl alcohol.

The method is carried out in the following sequence:
- 1-5% bran is added to the post-alcohol bard and the mixture is subjected to heat treatment at 70-90 ^o C for 1 hour with stirring;
- 0.5-5% alcohol condensate is added to the obtained nutrient medium;
- then sources of nitrogen and phosphorus (ammophos, diammophos or mono- or disubstituted potassium phosphate) are introduced in an amount of 80 mg of nitrogen and 45 mg of phosphorus per 1 g of protein;
- the process of growing yeast is carried out in an apparatus with aeration and stirring at a temperature of 30-40 ^o C and a pH of 5.0-5.5 in batch mode (growing time 8-12 hours) or in continuous mode at D = 0.14-0 2 hours ^-1 .

The technical result achieved by the implementation of the invention includes:
- disposal of post-alcohol distillery stillage (hydrolysis or grain), which is a waste of alcohol production;
- obtaining a feed product during the cultivation of yeast on bard with the addition of bran and alcohol condensate;
- with a continuous process of cultivating yeast on a post-alcohol grain stillage with bran and alcohol condensate, the productivity was 6.9-7.6 kg / m ³ / h with a crude protein content of 49.7-50%;
- with a continuous process of culturing yeast on a post-alcohol hydrolysis vinasse, the productivity was 4.8-5.8 kg / m ³ / h with a crude protein content of at least 40%.

 Information confirming the possibility of implementing the method.

 Example 1

2 or 5% of wheat bran was added to the post-alcohol hydrolysis vinasse (dry weight 2%) and the mixture was subjected to heat treatment at 70-90 ^° C for 1 hour with stirring. After cooling to 30-35 ^o C, 0.5 or 5% alcohol condensate was added to the mixture, as well as 8 g / l of potassium phosphate monosubstituted and 4 ml / l of a 20% solution of ammonium sulfuric acid. The pH of the medium was adjusted to 5.0 or 5.5.

The process of growing yeast on the prepared three-component nutrient medium was carried out in an apparatus for intensive mass transfer with aeration of 1 liter of air / liter of medium and with stirring (1000 rpm) at a temperature of 32-34 ^o C, pH 5.0-5.5, at D = 0.14 h ^-1 .

 The control was the process of growing yeast on a two-component substrate (hydrolysis stillage + wheat bran). The results are presented in table 1.

From the table. Figure 1 shows that when alcohol condensate was added to a two-component substrate, the growth rate increased from 0.1 to 0.14 h ^-1 ; productivity increased from 3.4 kg / m ³ / h to 4.8 and 5.8 kg / m ³ / h depending on the concentration of added alcohol condensate.

 Example 2

 The cultivation process was carried out as in example 1, but instead of the hydrolysis vinasse, post-alcohol grain vinasse (dry weight 5.7%) was used.

 The results are presented in table.2.

From the table. 2 shows that when adding alcohol condensate to a two-component substrate, the growth rate of yeast increased from 0.1 to 0.14 and 0.2 h ^-1 ; productivity increased from 4.9 to 6.9 and 9.8 kg / m ³ / h depending on the concentration of added alcohol condensate.

 Thus, the effectiveness of the proposed method for producing a protein feed product consists in using a three-component mixed substrate as a new nutrient medium, including post-alcohol distillery stillage (hydrolysis or grain), wheat bran and alcohol condensate.

When growing yeast on a hydrolysis bard with bran and alcohol condensate, the productivity was 4.8-5.8 kg / m ³ / h with a crude protein content in the finished product of 41.3-43.4% depending on the content of alcohol condensate in the medium.

When growing yeast on a grain stillage with bran and alcohol condensate, the productivity was 6.9-9.8 kg / m ³ / h with a crude protein content of 50% in the finished product.

 The present invention is new, since obtaining a protein feed product by the above method is not known from the prior art.

Claims (5)

 1. A method of obtaining a protein feed product, including the preparation of a nutrient medium, growing yeast on this medium, characterized in that the nutrient medium consists of three components - post-alcohol distillery stillage (hydrolysis or grain), wheat bran and alcohol condensate containing 20% ethyl alcohol.  2. The method according to claim 1, characterized in that the alcohol condensate is added in an amount of 0.5-5 vol.%. 3. The method according to PP.1 and 2, characterized in that the sum of the concentrations of carbon sources in the initial three-component nutrient medium with hydrolysis vinasse is 15-52 kg / m ³ , the time of cultivation of yeast is 8-12 hours 4. The method according to claims 1 and 2, characterized in that the sum of the concentrations of carbon sources in the initial three-component nutrient medium with grain stillage is 58.6-95.5 kg / m ³ , the cultivation time is 8-12 hours  5. The method according to claims 1, 3 and 4, characterized in that the ratio of the components in the nutrient medium is the post-alcohol distillery stillage: wheat bran: alcohol condensate = 200: (2-10) :( 1-10).

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