RU2265366C2 - Bioactive feed additive for farm and domestic animals and poultry for elevation of youngster preservation, improvement of feed digestion, animal growth and fertility stimulation - Google Patents

Abstract

FIELD: veterinary, in particular feed production for farm and domestic animals and poultry.

SUBSTANCE: fees additive composition contains biomass of physiologically active purine producers and enzymes Bac.subtilus and Bac.lichenifomis, respectively in ratio of 50:50. Moreover additive includes mixture of phosphorilated sugars (e.g. fructose, or lactose, or glucose, or sucrose) in form of calcium salts, as well as organic tricarboxylic acids such as fumaric and succinic acid, citric acid, ascorbic acid or salts thereof, and vitamin B₁₂. Method of present invention makes it possible to increase fur animal fertility by 11 % (mink), by 15 % (sable), to increase calf youngster preservation by 10-12 %, decrease feed for hen youngster conversion by 11.5 %.

EFFECT: high quality feed additive.

2 cl, 3 tbl, 3 ex

Description

The invention relates to veterinary medicine and can be used in the manufacture of feed for farm animals, domestic animals and poultry with the aim of increasing their digestibility, giving them the properties of protecting young animals from diseases, stimulating growth processes and increasing the fecundity of animals and birds.

Our drug Purivetin is well known (RF Patent No. 2195838, Reg. 10.01.03), which contains riboxin (inosine), fructose-1,6-diphosphate of calcium, vitamin B ₁₂ , vitamin C (ascorbic acid), lemon acid.

Further improvement of the preparation technology was associated with the exclusion of methyluracil from the mixture due to its possible carcinogenic properties, an increase in the concentration of riboxin (inosine), the introduction of succinic acid and calcium glycerophosphate. This composition (application No. 2003116138 dated 02.06.03) had a number of advantages and mainly the property of a significant increase in metabolic processes. However, the effect of reducing the feed conversion ratio was not always achieved; in some cases, diarrhea in calves arose in a rather active form. In addition, the substances that make up Purivetin, such as riboxin, fructose-1,6-diphosphate of calcium, calcium glycerophosphate, are purified pharmaceutical products and therefore have a high cost, which creates economic difficulties in the implementation of Purivetin.

Based on the foregoing, we have developed a composition of biologically active additives with the ability to more pronounced immune defense, strengthening both sides of the metabolism - catabolism and anabolism, and in this regard, better digestibility of feed. In addition, an important task was to reduce the cost of raw materials, in this regard, the study of the possibility of using veterinary substances, more affordable and cheaper.

The development was based on the idea of maintaining a balance of the processes of catabolism and anabolism in the animal organism at a high level.

Since activation of the processes of catabolism is primarily associated with an increase in the rate of oxidation of food substrates while achieving optimal feed conversion conditions, we set the task of normalizing the intestinal microflora, while also pursuing the goal of enhancing the body's immune response.

For this purpose, the dried biomass of You was used as part of the formulation. subtilis - producer of nucleosides and nucleotides (inosine, guanosine, hypoxanthine, inosinic acid, guanilic acid) and you. licheniformis - producer of substrate oxidizing enzymes (gluconase, xylonase, etc.).

It is important that during the germination of the spore biomass material in the intestine, the mentioned metabolites are also formed, which stimulate the development of normal intestinal microflora. The most optimal was the biomass ratio of you. subtilis and you. lichenifomis in the mixture - 50% each. Thus, we have established that the main catabolic activity can be provided by a mixture of producers - cultures (you. Subtilis and you. Lichenifomis), which form biologically active substances (nucleosides, nucleotides, enzymes). These products are affordable because there is no need for multi-stage and expensive cleaning.

The next stage of the work was the search for compounds that enhance the catabolism reactions, in particular, CTK (tricarboxylic acid cycle) and oxidative phosphorylation system. The best biologically active substances were vitamin B ₁₂ , fumaric acid or its salts, succinic acid or its salts, citric acid or its salts and ascorbic acid or its salts. The combination of the found optimal concentrations of these compounds with the biomass of you. subtilis and you. lichenifomis in the composition of the supplement allowed not only to significantly enhance the immunity and survival of young animals, but also to improve blood counts and digestibility of feed.

Further studies were aimed at enhancing the reactions of anabolism in animals (gluconeogenesis).

In this aspect, a number of substances were investigated and their role at different stages of gluconeogenesis was studied. First of all, we examined phosphorylated sugars obtained by sucrose brewing yeast transformation. A mixture of sugars — glucose, fructose, lactose, and preferably calcium salts of phosphorylated sugars, individually or in a mixture, turned out to be good substrates for activating anabolism reactions in animals and birds.

From an economic point of view, it is important to obtain a mixture of calcium salts of Sugars, since the isolation of pure water-soluble sodium salts is a costly process for using them as feed additives. Thus, the developed composition of biologically active additives, consisting of dried biomass of you. subtilis and you. lichenifomis in a ratio of 50:50 and a mixture of organic acids (citric, succinic, fumaric and ascorbic) and phosphorylated Sugars, vitamin B ₁₂ , turned out to be an extremely effective regulator of metabolic processes in the body with the functions of activating protein synthesis, nucleic acids, energy, maintaining the optimal ratio of elements blood, the concentration of glucose in the blood and oxygen, which also has a hepatoprotective effect.

The supplement has been tested on all farm and domestic animals and poultry and has been shown to be highly effective.

Examples

The stimulating effect of biologically active additives on the livelihoods of farm animals, fur animals and birds is confirmed, in particular:

increase the safety of young calves;

increased fertility of animals (fur animals).

Example 1

The influence of the biologically active additive on the safety of young calves was studied in March-April 2002 on the basis of the Breeding Plant of the Leningrad Region.

Tested the biologically active additive of the following composition: you biomass. subtilis - producer of nucleosides and nucleotites (inosine, inosinic acid, guanosine, guanilic acid) and the biomass of you. licheniformis - producer of substrate oxidizing enzymes (xylonase, gluconase) in a ratio of 50:50, fumaric and succinic acids in a ratio of 1: 3 and a mixture of phosphorylated sugars in the form of calcium salts, vitamin B ₁₂ . The supplement also contained citric and ascorbic acids. Formed 2 age groups of 60 goals in each group:

Group 1 - calves kept in the parental unit from birth to 15 days of age;

Group 2 - calves kept on calves from 15 days of age to two months of age.

Accordingly, 2 control groups were formed. A dietary supplement was used daily with milk once a day at the rate of 30 mg / kg calf live weight. The results are presented in table 1.

Table 1
The effect of biologically active additives on the safety of calves 1 group 2 group Indicators rev. experience the control experience the control The number of calves participating in the experiment Goal 60 60 60 60 The number of sick calves Goal 19 54 thirty 53 The number of sick calves % 32 90 fifty 83 Young growth Goal 0 6 1 8 Young growth % 0 10 0.6 thirteen

Thus, in the experimental groups, the number of sick calves and losses is less than in the control.

Example 2

The study of the influence of biologically active additives on the fecundity of fur animals (mink, sable) was carried out on the basis of the experimental farm NIIPZK them. Afanasyeva (Ramensky district, Moscow region).

We used a biologically active supplement containing you biomass. subtilis - producer of nucleosides and nucleotides (inosine, guanosine, inosinic and guanilic acid) and the biomass of you. licheniformis is a producer of substrate oxidizing enzymes in a ratio of 50:50, as well as fumaric and succinic acids in a ratio of 1: 3 and calcium salts of phosphorylated sugars.

The supplement also contained citric acid, vitamin C and vitamin B ₁₂ .

For the experiment, two groups (experience, control) of minks and sable were formed.

Group 1 - control, animals were kept on the diet without the introduction of biologically active additives;

Group 2 - experience, animals received a dietary supplement of 30 mg / kg body weight of the animal every 10 days (10 days a dietary supplement, 10 days a break) for four months.

Table 2 presents the results of the experiments.

table 2
The effect of biologically active additives on the fecundity of fur animals Indicators Mink (wild color) Sable the control experience The control experience Total females, goals 73 75 68 65 Covered with females,% 100 100 100 100 Goal, goals 66 70 33 40 Females,% 90,4 93 48.5 74,4 Live puppies 368 433 110 153 Fertility 5.72 ± 0.26 6.18 ± 0.23 3.35 ± 0.5 3.8 ± 0.4 Fertility,% 100 111 100 115

Thus, when using a biologically active additive, the fertility in fur animals is higher than in the control, by 11% in minks and 15% in sables.

Example 3

The study of the influence of biologically active additives on the safety of young chickens and feed conversion was carried out on the basis of LLC Novaya Nevskaya sub-farm of the Leningrad Region.

We used a biologically active supplement containing you biomass. subtilis - producer of nucleosides and nucleotides (inosine, guanosine, inosinic acid, guanilic acid) and the biomass of you. licheniformis is a producer of substrate-oxidizing enzymes in a ratio of 50:50, as well as fumaric and succinic acids in a ratio of 1: 3, a mixture of calcium salts of phosphorylated sugars. The supplement also contained citric acid, vitamin C and vitamin B ₁₂ .

Formed 2 groups (control and experience) of 260 one-day chickens in each group.

Both groups were kept under the same conditions, but a biologically active additive at a dose of 1 g per 1 kg of feed was added to the young feed of the experimental group.

The experiment was continued until 120 days of age.

The results of the experiment are shown in table 3.

Table 3
The effect of biologically active additives on the safety of young chickens and feed conversion Indicators the control experience The number of goals of young chickens 160 160 The safety of young chickens,% 92 100 Feed costs in kg per 1 kg of growth 9.6 8.5 Feed conversion,% 100 88.5

Thus, the biologically active supplement had a positive effect on the safety of young chickens (on average by 8%) and on feed conversion (decrease on average by 11.5%).

Claims (2)

1. Biologically active feed additive for agricultural, domestic animals and poultry to increase the safety of young animals, increase feed digestibility, stimulate growth processes and fertility, containing dried biomass producer Bac.subtilis as a source of substrate-oxidizing enzymes as a source of nucleosides and nucleotides - dried biomass producer Bac.lichenifomis, and the dried biomass Bac.subtilis and Bac.lichenifomis taken in a ratio of 50:50, as an activator of glycolysis, sugar and additionally B ₁₂ Tamin, fumaric, succinic, citric and ascorbic acids or salts thereof. 2. The dietary supplement according to claim 1, characterized in that fructose, or sucrose, or glucose, or lactose, or mixtures thereof or their phosphorylated forms in the form of calcium salts, obtained by the method of yeast transformation of sugars, for example fructose, are used as sugars. 1,6-diphosphate of calcium.