RU2240127C2 - Method for preparing preparation for activation of body immune system - Google Patents

Abstract

FIELD: veterinary pharmacology.

SUBSTANCE: method for preparing a preparation for activation of immune system of body involves mixing 100 mas. p. p. of Dorogov's antiseptic-stimulator with 10 mas. p. p. of ascorbic acid and additional addition of para-aminobenzoic acid in the amount 1.20-1.25 mas. p., 0.1 N hydrochloric acid solution in the amount 100 mas. p. p. and formalin in the amount 0.5 mas. p. Method provides expanding assortment of agents used for activation of the body immune system.

EFFECT: improved preparing method.

1 tbl, 2 ex

Description

The invention relates to the field of biology and veterinary medicine, and in particular to methods for producing preparations for activating the immune system of an animal organism.

A known method of obtaining an immunostimulant, which consists in extraction with a 3% solution of acetic acid with the addition of zinc chloride for 24-48 hours of salmon fish milk. The extract is purified by centrifugation and filtration through a porous material [1].

The disadvantages of the method include the scarcity of raw materials and the use of complex hardware design with the cleaning system of the resulting immunostimulant.

The closest in technical essence and the achieved result is a method of producing an immunostimulant, comprising mixing pre-prepared aqueous phases of an antiseptic ASD and ascorbic acid at pH 6.0-7.5, and then the oil phase by heating mineral oil and an emulsifier, followed by emulsification of both phases [ 2].

Known drug increases the activity of antibodies producing cells. The disadvantages of the drug include a slightly high stimulating activity and the inability to use them for parenteral use, since ASD causes tissue necrosis, and the oil suspension is poorly absorbed and has a negative effect.

The invention is aimed at obtaining a drug expanding the range of agents that increase the activity of the body’s immune system, namely increasing the number of antibodies producing cells, increasing the processes of protein synthesis, the level of gamma globulins, immunoglobulins in animals.

The technical result achieved by the implementation of the claimed invention is to expand the range of products that increase the activity of the body's immune system.

This is achieved by the way a preparation for activating the body’s immune system, comprising mixing Dorogov antiseptic stimulator and ascorbic acid, characterized in that 100 parts by weight are mixed. Dorogov antiseptic stimulator with 10 parts by weight ascorbic acid and additionally injected paraaminobenzoic acid in an amount of 1.20-1.25 parts by weight, 0.1 N hydrochloric acid solution in an amount of 100 parts by weight and formalin, in an amount of 0.5 wt.h.

The optimal amount of para-aminobenzoic acid and hydrochloric acid are the indicated amounts. The amount is less than 1.20 parts by weight. not enough to achieve a technical result, and the amount is more than 1.25 parts by weight depresses the body's immune system.

The method is as follows.

Example 1

100 parts by weight Dorogov's antiseptic stimulator (fraction - 2) was mixed with 1.25 parts by weight of paraaminobenzoic acid, 10 parts by weight ascorbic acid. To the resulting mixture, 100 parts by weight were added with constant stirring. 0.1 N hydrochloric acid solution and 0.5 wt.h. formalin to a pH of a mixture of 7.0-7.2. The prepared preparation was poured into 100-200 ml vials, closed with stoppers and rolled up with aluminum caps.

Example 2

The method was carried out analogously to example 1. Dorogov antiseptic stimulator (fraction - 2) was mixed with 1.20 wt.h. para-aminobenzoic acid.

To implement the method used the following substances:

1. Dorogov antiseptic stimulator TU 10197389

2. Para-aminobenzoic acid TU 6-09-3395-78

3. Ascorbic acid TU 6-09-07-1136-78

4. Hydrochloric acid GOST 3118-77

5. Formalin GOST 1625

The difference between the proposed solution and the known one is that when para-aminobenzoic acid and 0.1 N hydrochloric acid are introduced together with ASD and ascorbic acid, it is neutralized, which eliminates tissue necrosis during subcutaneous or intramuscular injection, and also eliminates the use of an oil mixture , which is poorly absorbed by subcutaneous parenteral administration of the drug.

The test of the drug was carried out according to the principle of steam analogues. Three groups of calves were selected with 10 goals in each, the first group of calves was injected with a preparation prepared according to the prototype, the second group with the preparation of the invention at a dose of 0.1 ml / kg (experimental version), and the third group with a control.

On the 30th day, the phagocytic activity, lysozyme activity, bactericidal activity, total protein, protein fractions and immunoglobulins were determined. The data are given in the table.

Parameters of nonspecific resistance of the body of calves at their 30 days of age.

Thus, on the basis of the analysis of the parameters of the immunological and biochemical profile of the body, it was found that intramuscular injection of the proposed drug improves the indicators of non-specific resistance of animals.

Sources of information

1. RU 2091073, class A 61 K 35/60.

2. A.S. No. 1759424, cl. A 61 K 35/00, 09/07/92.

Claims (1)

A method of obtaining a drug to activate the body's immune system, comprising mixing a Dorogov antiseptic stimulator and ascorbic acid, characterized in that 100 parts by weight are mixed. Dorogov antiseptic stimulator with 10 parts by weight ascorbic acid and additionally introduce para-aminobenzoic acid in an amount of 1.20-1.25 parts by weight, 0.1N hydrochloric acid solution in an amount of 100 parts by weight and formalin in an amount of 0.5 wt.h.