RU2019137083A - Линкеры в виде короткой боковой ручки для нуклеотидов в областях применения секвенирования - Google Patents
Линкеры в виде короткой боковой ручки для нуклеотидов в областях применения секвенирования Download PDFInfo
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- RU2019137083A RU2019137083A RU2019137083A RU2019137083A RU2019137083A RU 2019137083 A RU2019137083 A RU 2019137083A RU 2019137083 A RU2019137083 A RU 2019137083A RU 2019137083 A RU2019137083 A RU 2019137083A RU 2019137083 A RU2019137083 A RU 2019137083A
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- compound according
- compound
- nucleotides
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- 239000002773 nucleotide Substances 0.000 title claims 12
- 150000001875 compounds Chemical class 0.000 claims 26
- 125000003729 nucleotide group Chemical group 0.000 claims 11
- 108091034117 Oligonucleotide Proteins 0.000 claims 10
- 238000003556 assay Methods 0.000 claims 8
- 238000009396 hybridization Methods 0.000 claims 3
- 238000000159 protein binding assay Methods 0.000 claims 3
- 238000012163 sequencing technique Methods 0.000 claims 3
- CZPWVGJYEJSRLH-UHFFFAOYSA-N Pyrimidine Chemical group C1=CN=CN=C1 CZPWVGJYEJSRLH-UHFFFAOYSA-N 0.000 claims 2
- 238000004458 analytical method Methods 0.000 claims 2
- 230000002068 genetic effect Effects 0.000 claims 2
- 125000002264 triphosphate group Chemical group [H]OP(=O)(O[H])OP(=O)(O[H])OP(=O)(O[H])O* 0.000 claims 2
- 238000010195 expression analysis Methods 0.000 claims 1
- 238000012252 genetic analysis Methods 0.000 claims 1
- 239000002777 nucleoside Substances 0.000 claims 1
- 125000003835 nucleoside group Chemical group 0.000 claims 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/06—Pyrimidine radicals
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/06—Pyrimidine radicals
- C07H19/073—Pyrimidine radicals with 2-deoxyribosyl as the saccharide radical
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/06—Pyrimidine radicals
- C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/14—Pyrrolo-pyrimidine radicals
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/16—Purine radicals
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/16—Purine radicals
- C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Chemical Kinetics & Catalysis (AREA)
Claims (40)
1. Соединение формулы (I):
где В представляет собой нуклеозидное основание; и
Fl представляет собой флуорофор, присоединенный через возможный линкер.
2. Соединение по п. 1, где В представляет собой нуклеотидное основание.
3. Соединение по п. 2, где В представляет собой пиримидиновое основание.
4. Соединение по п. 3, где указанное соединение имеет формулу (с) или формулу (t):
где р представляет собой трифосфатную группу; и
Fl представляет собой флуорофор, присоединенный через возможный линкер.
5. Соединение по п. 2, где В представляет собой 7-деазапуриновое основание.
6. Соединение по п. 5, где указанное соединение имеет формулу (а):
где р представляет собой трифосфатную группу; и
Fl представляет собой флуорофор, присоединенный через возможный линкер.
7. Соединение по п. 2, где указанное соединение присоединено к олигонуклеотиду.
8. Олигонуклеотид, меченный соединением по п. 2.
9. Соединение формулы (II):
где В представляет собой нуклеотидное основание.
10. Соединение по п. 9, где В представляет собой пиримидиновое основание.
11. Соединение по п. 10, где указанное соединение имеет формулу (ci) или формулу (ti):
где Oln представляет собой олигонуклеотид.
12. Соединение по п. 9, где В представляет собой 7-деазапуриновое основание.
13. Соединение по п. 12, где указанное соединение имеет формулу (ai):
где Oln представляет собой олигонуклеотид.
14. Олигонуклеотид, содержащий соединение по любому из пп. 9-13.
15. Олигонуклеотид, содержащий две или более копий соединения по любому из пп. 9-13.
16. Олигонуклеотид, содержащий десять или более копий соединения по любому из пп. 9-13.
17. Олигонуклеотид, содержащий сто или более копий соединения по любому из пп. 9-13.
18. Набор, содержащий два или более нуклеотидов, где по меньшей мере один нуклеотид представляет собой меченый нуклеотид, который представляет собой соединение по любому из пп. 2-6.
19. Набор по п. 18, содержащий два или более нуклеотидов, где по меньшей мере два нуклеотида представляют собой меченые нуклеотиды, которые представляют собой соединения по любому из пп. 2-6.
20. Набор по п. 18, содержащий четыре нуклеотида, где по меньшей мере два нуклеотида представляют собой меченые нуклеотиды, которые представляют собой соединения по любому из пп. 2-6.
21. Применение соединения по любому из пп. 2-6 в секвенировании, анализе экспрессии, гибридизационном анализе, генетическом анализе, анализе РНК или анализах связывания белка, или их комбинациях.
22. Применение олигонуклеотида по п. 8 в секвенировании, анализе экспрессии, гибридизационном анализе, генетическом анализе, анализе РНК или анализах связывания белка, или их комбинациях.
23. Применение олигонуклеотида по п. 14 в секвенировании, анализе экспрессии, гибридизационном анализе, генетическом анализе, анализе РНК или анализах связывания белка, или их комбинациях.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB1711219.4 | 2017-07-12 | ||
| GBGB1711219.4A GB201711219D0 (en) | 2017-07-12 | 2017-07-12 | Short pendant arm linkers for nucleotides in sequencing applications |
| PCT/EP2018/069030 WO2019012080A1 (en) | 2017-07-12 | 2018-07-12 | SHORT ARM LINKS FOR NUCLEOTIDES IN SEQUENCING APPLICATIONS |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU2019137083A3 RU2019137083A3 (ru) | 2021-06-18 |
| RU2019137083A true RU2019137083A (ru) | 2021-06-18 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2019137083A RU2019137083A (ru) | 2017-07-12 | 2018-07-12 | Линкеры в виде короткой боковой ручки для нуклеотидов в областях применения секвенирования |
Country Status (14)
| Country | Link |
|---|---|
| US (4) | US10526648B2 (ru) |
| EP (1) | EP3652191A1 (ru) |
| JP (1) | JP7041695B2 (ru) |
| KR (2) | KR20200023282A (ru) |
| CN (1) | CN111094315B (ru) |
| AU (1) | AU2018298847B2 (ru) |
| CA (1) | CA3060152A1 (ru) |
| GB (1) | GB201711219D0 (ru) |
| IL (1) | IL271428A (ru) |
| MX (2) | MX386201B (ru) |
| MY (1) | MY196912A (ru) |
| RU (1) | RU2019137083A (ru) |
| WO (1) | WO2019012080A1 (ru) |
| ZA (1) | ZA201906836B (ru) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB201711219D0 (en) | 2017-07-12 | 2017-08-23 | Illumina Cambridge Ltd | Short pendant arm linkers for nucleotides in sequencing applications |
| KR20210098844A (ko) | 2018-12-05 | 2021-08-11 | 일루미나, 인코포레이티드 | 중합효소, 조성물, 및 사용 방법 |
| AU2020224097B2 (en) * | 2019-02-19 | 2024-03-07 | Ultima Genomics, Inc. | Linkers and methods for optical detection and sequencing |
| EP3931190A1 (en) | 2019-03-01 | 2022-01-05 | Illumina Cambridge Limited | Tertiary amine substituted coumarin compounds and their uses as fluorescent labels |
| KR102829192B1 (ko) | 2019-03-01 | 2025-07-04 | 일루미나 케임브리지 리미티드 | 엑소사이클릭 아민 치환된 쿠마린 화합물 및 형광 표지로서의 이의 용도 |
| AU2020389779A1 (en) | 2019-11-27 | 2022-05-26 | Illumina Cambridge Limited | Cyclooctatetraene containing dyes and compositions |
| EP4317168A4 (en) * | 2021-04-01 | 2025-08-27 | Bgi Shenzhen | NUCLEOTIDE ANALOGUE FOR SEQUENCING |
| US12077789B2 (en) | 2021-08-14 | 2024-09-03 | Illumina, Inc. | Polymerases, compositions, and methods of use |
| CN116239493B (zh) * | 2023-03-15 | 2024-07-26 | 杭州易速微控基因技术有限公司 | 一种Linker类化合物的合成方法 |
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| ATE364718T1 (de) | 1997-04-01 | 2007-07-15 | Solexa Ltd | Verfahren zur vervielfältigung von nukleinsäure |
| US6465178B2 (en) | 1997-09-30 | 2002-10-15 | Surmodics, Inc. | Target molecule attachment to surfaces |
| US6485944B1 (en) | 1997-10-10 | 2002-11-26 | President And Fellows Of Harvard College | Replica amplification of nucleic acid arrays |
| DE69928265T3 (de) | 1998-07-30 | 2013-11-28 | Illumina Cambridge Ltd. | Matrizen von biomolekülen und ihre verwendung in sequenzierung |
| GB0002310D0 (en) | 2000-02-01 | 2000-03-22 | Solexa Ltd | Polynucleotide sequencing |
| AR021833A1 (es) | 1998-09-30 | 2002-08-07 | Applied Research Systems | Metodos de amplificacion y secuenciacion de acido nucleico |
| US6391937B1 (en) | 1998-11-25 | 2002-05-21 | Motorola, Inc. | Polyacrylamide hydrogels and hydrogel arrays made from polyacrylamide reactive prepolymers |
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| EP1307414A2 (en) | 2000-08-09 | 2003-05-07 | Amersham Biosciences AB | The use and evaluation of a 2+2] photocycloaddition in immobilization of oligonucleotides on a three-dimensional hydrogel matrix |
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| US7057026B2 (en) | 2001-12-04 | 2006-06-06 | Solexa Limited | Labelled nucleotides |
| ES2550513T3 (es) | 2002-08-23 | 2015-11-10 | Illumina Cambridge Limited | Nucleótidos modificados para secuenciación de polinucleótidos |
| US11008359B2 (en) * | 2002-08-23 | 2021-05-18 | Illumina Cambridge Limited | Labelled nucleotides |
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- 2017-07-12 GB GBGB1711219.4A patent/GB201711219D0/en not_active Ceased
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2018
- 2018-07-11 US US16/033,118 patent/US10526648B2/en active Active
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- 2018-07-12 KR KR1020197036418A patent/KR20200023282A/ko not_active Ceased
- 2018-07-12 KR KR1020227028169A patent/KR20220119182A/ko not_active Ceased
- 2018-07-12 WO PCT/EP2018/069030 patent/WO2019012080A1/en not_active Ceased
- 2018-07-12 JP JP2019570949A patent/JP7041695B2/ja active Active
- 2018-07-12 CA CA3060152A patent/CA3060152A1/en active Pending
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- 2018-07-12 AU AU2018298847A patent/AU2018298847B2/en active Active
- 2018-07-12 RU RU2019137083A patent/RU2019137083A/ru not_active Application Discontinuation
- 2018-07-12 CN CN201880041116.6A patent/CN111094315B/zh active Active
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Also Published As
| Publication number | Publication date |
|---|---|
| RU2019137083A3 (ru) | 2021-06-18 |
| CA3060152A1 (en) | 2019-01-17 |
| BR112019027614A2 (pt) | 2020-07-07 |
| NZ759350A (en) | 2023-10-27 |
| US20210230687A1 (en) | 2021-07-29 |
| EP3652191A1 (en) | 2020-05-20 |
| CN111094315B (zh) | 2024-04-09 |
| MY196912A (en) | 2023-05-10 |
| KR20200023282A (ko) | 2020-03-04 |
| US11655502B2 (en) | 2023-05-23 |
| KR20220119182A (ko) | 2022-08-26 |
| US20200131569A1 (en) | 2020-04-30 |
| MX386201B (es) | 2025-03-18 |
| AU2018298847A1 (en) | 2019-11-07 |
| US12071662B2 (en) | 2024-08-27 |
| US20190017111A1 (en) | 2019-01-17 |
| US11001888B2 (en) | 2021-05-11 |
| US10526648B2 (en) | 2020-01-07 |
| JP2020527131A (ja) | 2020-09-03 |
| CN111094315A (zh) | 2020-05-01 |
| MX2021011019A (es) | 2021-10-14 |
| AU2018298847B2 (en) | 2021-02-11 |
| JP7041695B2 (ja) | 2022-03-24 |
| GB201711219D0 (en) | 2017-08-23 |
| ZA201906836B (en) | 2023-04-26 |
| IL271428A (en) | 2020-01-30 |
| WO2019012080A1 (en) | 2019-01-17 |
| US20230348968A1 (en) | 2023-11-02 |
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