RU2018128142A - Способ получения l-метионина по ферментативной технологии - Google Patents
Способ получения l-метионина по ферментативной технологии Download PDFInfo
- Publication number
- RU2018128142A RU2018128142A RU2018128142A RU2018128142A RU2018128142A RU 2018128142 A RU2018128142 A RU 2018128142A RU 2018128142 A RU2018128142 A RU 2018128142A RU 2018128142 A RU2018128142 A RU 2018128142A RU 2018128142 A RU2018128142 A RU 2018128142A
- Authority
- RU
- Russia
- Prior art keywords
- methionine
- preceding paragraphs
- stage iii
- microorganism
- iii
- Prior art date
Links
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 title claims 10
- 230000002255 enzymatic effect Effects 0.000 title claims 4
- 238000004519 manufacturing process Methods 0.000 title claims 3
- 229960004452 methionine Drugs 0.000 claims 9
- 238000000034 method Methods 0.000 claims 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims 6
- FFEARJCKVFRZRR-UHFFFAOYSA-N L-Methionine Natural products CSCCC(N)C(O)=O FFEARJCKVFRZRR-UHFFFAOYSA-N 0.000 claims 6
- 229930195722 L-methionine Natural products 0.000 claims 6
- 239000002904 solvent Substances 0.000 claims 5
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 claims 3
- 229930182817 methionine Natural products 0.000 claims 3
- 244000005700 microbiome Species 0.000 claims 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims 2
- FFEARJCKVFRZRR-SCSAIBSYSA-N D-methionine Chemical compound CSCC[C@@H](N)C(O)=O FFEARJCKVFRZRR-SCSAIBSYSA-N 0.000 claims 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 claims 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims 1
- 102000004190 Enzymes Human genes 0.000 claims 1
- 108090000790 Enzymes Proteins 0.000 claims 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 claims 1
- 230000001476 alcoholic effect Effects 0.000 claims 1
- 229910052799 carbon Inorganic materials 0.000 claims 1
- 238000002425 crystallisation Methods 0.000 claims 1
- 230000008025 crystallization Effects 0.000 claims 1
- 239000001963 growth medium Substances 0.000 claims 1
- 238000002955 isolation Methods 0.000 claims 1
- 239000002609 medium Substances 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 229910052757 nitrogen Inorganic materials 0.000 claims 1
- 239000002243 precursor Substances 0.000 claims 1
- 229910052717 sulfur Inorganic materials 0.000 claims 1
- 239000011593 sulfur Substances 0.000 claims 1
- 239000006228 supernatant Substances 0.000 claims 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/12—Methionine; Cysteine; Cystine
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C319/00—Preparation of thiols, sulfides, hydropolysulfides or polysulfides
- C07C319/26—Separation; Purification; Stabilisation; Use of additives
- C07C319/28—Separation; Purification
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C323/00—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups
- C07C323/50—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups containing thio groups and carboxyl groups bound to the same carbon skeleton
- C07C323/51—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups containing thio groups and carboxyl groups bound to the same carbon skeleton having the sulfur atoms of the thio groups bound to acyclic carbon atoms of the carbon skeleton
- C07C323/57—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups containing thio groups and carboxyl groups bound to the same carbon skeleton having the sulfur atoms of the thio groups bound to acyclic carbon atoms of the carbon skeleton the carbon skeleton being further substituted by nitrogen atoms, not being part of nitro or nitroso groups
- C07C323/58—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups containing thio groups and carboxyl groups bound to the same carbon skeleton having the sulfur atoms of the thio groups bound to acyclic carbon atoms of the carbon skeleton the carbon skeleton being further substituted by nitrogen atoms, not being part of nitro or nitroso groups with amino groups bound to the carbon skeleton
Landscapes
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Claims (12)
1. Способ ферментативного получения L-метионина и/или его производных, включающий стадии:
i) выращивания микроорганизма, оптимизированного для ферментативного получения метионина, в соответствующей среде для выращивания, содержащей источник углерода, источник серы и источник азота, с получением L-метионина;
ii) выделения L-метионина, полученного на стадии i);
iii) добавления спиртового растворителя или ацетонитрила к содержащей метионин фракции, полученной на стадии ii), для осаждения L-метионина; и
iv) выделения L-метионина.
2. Способ по п. 1, в котором микроорганизм на стадии i) представляет собой единственный микроорганизм.
3. Способ по любому из предыдущих пунктов, который не включает проведение какой-либо ферментативной обработки предшественника L-метионина.
4. Способ по любому из предыдущих пунктов, в котором стадия ii) включает стадию осветления ферментативной среды с последующей стадией кристаллизации L-метионина или без нее.
5. Способ по любому из предыдущих пунктов, в котором спиртовой растворитель, использующийся на стадии iii), выбран из этанола, изопропанола или их смеси.
6. Способ по любому из предыдущих пунктов, в котором спиртовым растворителем, использующимся на стадии iii), является этанол.
7. Способ по любому из предыдущих пунктов, в котором на стадии iii) соотношение растворитель / (фракция содержащая метионин + растворитель) составляет от 50 до 80 мас. %, предпочтительно от 65 до 75%.
8. Способ по любому из предыдущих пунктов, в котором на стадии iii) остаточная растворимость L-метионина в надосадочной жидкости после проведения стадии iii) составляет от 5 до 6 г⋅л-1 при комнатной температуре.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/IB2016/000122 WO2017118871A1 (en) | 2016-01-08 | 2016-01-08 | Method to produce l-methionine by a fermentative production |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU2018128142A true RU2018128142A (ru) | 2020-02-10 |
| RU2018128142A3 RU2018128142A3 (ru) | 2020-02-10 |
Family
ID=55486985
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2018128142A RU2018128142A (ru) | 2016-01-08 | 2016-01-08 | Способ получения l-метионина по ферментативной технологии |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20180355390A1 (ru) |
| EP (1) | EP3400305A1 (ru) |
| KR (1) | KR20180093981A (ru) |
| CN (1) | CN108603207A (ru) |
| BR (1) | BR112018013873A2 (ru) |
| MX (1) | MX2018008129A (ru) |
| RU (1) | RU2018128142A (ru) |
| WO (1) | WO2017118871A1 (ru) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11882852B2 (en) | 2018-02-09 | 2024-01-30 | Haldor Topsøe A/S | Process of producing alpha-hydroxy compounds and uses thereof |
| KR102489243B1 (ko) * | 2020-09-11 | 2023-01-16 | 고려대학교 산학협력단 | S-메틸메티오닌 생산용 재조합 미생물 및 이를 이용한 s-메틸메티오닌의 제조방법 |
| WO2024175452A1 (en) * | 2023-02-22 | 2024-08-29 | Evonik Operations Gmbh | Apparatus and process for recovery of a valuable material |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6881837B2 (en) * | 2001-06-07 | 2005-04-19 | Orchid Chemicals & Pharmaceuticals Ltd. | Chemical synthesis of S-adenosyl-L-methionine with enrichment of (S,S)-isomer |
| DE10249642A1 (de) | 2002-10-24 | 2004-05-13 | Consortium für elektrochemische Industrie GmbH | Feedback-resistente Homoserin-Transsuccinylasen mit modifiziertem C-Terminus |
| US7252756B2 (en) * | 2002-12-18 | 2007-08-07 | Bp Corporation North America Inc. | Preparation of components for refinery blending of transportation fuels |
| WO2005007862A2 (en) | 2003-07-08 | 2005-01-27 | Novus Internation, Inc | Methionine recovery processes |
| DE10359668A1 (de) * | 2003-12-18 | 2005-07-14 | Basf Ag | Verfahren zur Herstellung von Methionin |
| WO2005111202A1 (en) | 2004-05-12 | 2005-11-24 | Metabolic Explorer | Recombinant enzyme with altered feedback sensitivity |
| KR100651220B1 (ko) | 2004-06-29 | 2006-11-29 | 씨제이 주식회사 | Ｌ-메씨오닌 생산 균주 및 상기 균주를 이용한ｌ-메씨오닌의 생산방법 |
| WO2006138689A2 (en) | 2005-06-17 | 2006-12-28 | Microbia, Inc. | Improved amino acid and metabolite biosynthesis |
| JP2009501550A (ja) | 2005-07-18 | 2009-01-22 | ビーエーエスエフ ソシエタス・ヨーロピア | メチオニン生産組換え微生物 |
| WO2007017710A1 (en) | 2005-08-11 | 2007-02-15 | Metabolic Explorer | Process for the preparation of aspartate and derived amino acids like lysine, threonine, isoleucine, methionine, homoserine, or valine employing a microorganism with enhanced isocitrate lyase and/or malate synthase expression |
| EP1969130B1 (en) | 2006-01-04 | 2014-03-12 | Metabolic Explorer | Process for the preparation of methionine and its precursors homoserine or succinylhomoserine employing a microorganism |
| WO2009043372A1 (en) | 2007-10-02 | 2009-04-09 | Metabolic Explorer | Increasing methionine yield |
| KR101164711B1 (ko) * | 2009-02-27 | 2012-07-11 | 씨제이제일제당 (주) | 미네랄 첨가와 산처리를 이용하여 메치오닌의 용해도를 증가시키는 방법 |
| FR2951195B1 (fr) | 2009-10-14 | 2014-01-31 | Roquette Freres | Composition riche en methionine destinee a l'alimentation animale |
| JP6368488B2 (ja) | 2010-12-29 | 2018-08-01 | シージェイ チェイルジェダン コーポレーション | Ｌ−メチオニン及び関連産物の生産方法 |
| EP2479279A1 (de) | 2011-01-20 | 2012-07-25 | Evonik Degussa GmbH | Verfahren zur fermentativen Herstellung schwefelhaltiger Aminosäuren |
| AR086790A1 (es) | 2011-06-29 | 2014-01-22 | Metabolic Explorer Sa | Un microorganismo para la produccion de metionina con importacion de glucosa mejorada |
| FR2983870B1 (fr) | 2011-12-08 | 2015-07-17 | Roquette Freres | Composition en methionine destinee a l'alimentation animale |
| ES2632170T3 (es) | 2012-06-18 | 2017-09-11 | Evonik Degussa Gmbh | Microorganismo recombinante para la producción fermentativa de metionina |
| KR20160044038A (ko) | 2013-08-30 | 2016-04-22 | 메타볼릭 익스플로러 | 메티오닌 유출이 증진된 메티오닌 생산용 미생물 |
| PL3039153T3 (pl) | 2013-08-30 | 2019-02-28 | Evonik Degussa Gmbh | Mikroorganizm do wytwarzania metioniny o ulepszonej aktywności syntazy metioniny i wypływie metioniny |
-
2016
- 2016-01-08 EP EP16708717.0A patent/EP3400305A1/en not_active Withdrawn
- 2016-01-08 RU RU2018128142A patent/RU2018128142A/ru unknown
- 2016-01-08 WO PCT/IB2016/000122 patent/WO2017118871A1/en not_active Ceased
- 2016-01-08 US US15/774,267 patent/US20180355390A1/en not_active Abandoned
- 2016-01-08 KR KR1020187019091A patent/KR20180093981A/ko not_active Withdrawn
- 2016-01-08 MX MX2018008129A patent/MX2018008129A/es unknown
- 2016-01-08 BR BR112018013873A patent/BR112018013873A2/pt not_active Application Discontinuation
- 2016-01-08 CN CN201680077838.8A patent/CN108603207A/zh active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| KR20180093981A (ko) | 2018-08-22 |
| EP3400305A1 (en) | 2018-11-14 |
| MX2018008129A (es) | 2018-12-06 |
| BR112018013873A2 (pt) | 2018-12-18 |
| US20180355390A1 (en) | 2018-12-13 |
| RU2018128142A3 (ru) | 2020-02-10 |
| CN108603207A (zh) | 2018-09-28 |
| WO2017118871A1 (en) | 2017-07-13 |
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