RU2013106976A - Set of synthetic oligonucleotides for amplification and sequencing of ITS1-5.8S-ITS2 VASCULAR PLANTS - Google Patents
Set of synthetic oligonucleotides for amplification and sequencing of ITS1-5.8S-ITS2 VASCULAR PLANTS Download PDFInfo
- Publication number
- RU2013106976A RU2013106976A RU2013106976/10A RU2013106976A RU2013106976A RU 2013106976 A RU2013106976 A RU 2013106976A RU 2013106976/10 A RU2013106976/10 A RU 2013106976/10A RU 2013106976 A RU2013106976 A RU 2013106976A RU 2013106976 A RU2013106976 A RU 2013106976A
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- RU
- Russia
- Prior art keywords
- its2
- its1
- sequencing
- amplification
- synthetic oligonucleotides
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- 108091034117 Oligonucleotide Proteins 0.000 title claims abstract 3
- 241000592342 Tracheophyta Species 0.000 title claims abstract 3
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 title claims abstract 3
- 230000003321 amplification Effects 0.000 title claims abstract 3
- 238000003199 nucleic acid amplification method Methods 0.000 title claims abstract 3
- 238000012163 sequencing technique Methods 0.000 title claims abstract 3
- 108091028043 Nucleic acid sequence Proteins 0.000 claims abstract 2
- 239000002773 nucleotide Substances 0.000 claims abstract 2
- 125000003729 nucleotide group Chemical group 0.000 claims abstract 2
- 238000003752 polymerase chain reaction Methods 0.000 claims abstract 2
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Набор синтетических олигонуклеотидов для амплификации и секвенирования ITS1-5.8S-ITS2 сосудистых растений, включающий проведение полимеразной цепной реакции с помощью универсальных праймеров, отличающийся тем, что в качестве праймеров используются последовательности ДНК со следующим нуклеотидным составом:primer ITS-for CGTAACAAGGTTTCCGTAGprimer ITS-rew GGAATCCTTGTAAGTTTCTTT.A set of synthetic oligonucleotides for amplification and sequencing ITS1-5.8S-ITS2 vascular plants, comprising carrying out a polymerase chain reaction using universal primers, wherein the primers are used as a DNA sequence with the following nucleotide composition: primer ITS-for CGTAACAAGGTTTCCGTAGprimer ITS-rew GGAATCCTTGTAAGTTTCTTT .
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2013106976/10A RU2528063C1 (en) | 2013-02-18 | 2013-02-18 | Set of synthetic oligonucleotides for amplification and sequencing its1-5,8s-its2 of vascular plants |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2013106976/10A RU2528063C1 (en) | 2013-02-18 | 2013-02-18 | Set of synthetic oligonucleotides for amplification and sequencing its1-5,8s-its2 of vascular plants |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU2013106976A true RU2013106976A (en) | 2014-08-27 |
| RU2528063C1 RU2528063C1 (en) | 2014-09-10 |
Family
ID=51455910
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2013106976/10A RU2528063C1 (en) | 2013-02-18 | 2013-02-18 | Set of synthetic oligonucleotides for amplification and sequencing its1-5,8s-its2 of vascular plants |
Country Status (1)
| Country | Link |
|---|---|
| RU (1) | RU2528063C1 (en) |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2113481C1 (en) * | 1996-08-28 | 1998-06-20 | Институт общей генетики им.Н.И.Вавилова РАН | Method of investigation of structural-functional organization of dna of eucaryotic ribosomal claster |
| CN101348833B (en) * | 2008-07-16 | 2010-12-01 | 江苏同源堂生物工程有限公司 | ITS sequence of Tremella aurantiablba Bandoni and Stereum hirsutum and method for identifying golden fungus using the same |
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2013
- 2013-02-18 RU RU2013106976/10A patent/RU2528063C1/en not_active IP Right Cessation
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| Publication number | Publication date |
|---|---|
| RU2528063C1 (en) | 2014-09-10 |
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| MM4A | The patent is invalid due to non-payment of fees |
Effective date: 20180219 |