RU2006126404A - MEANS OF "ECDISTERON-80" Possessing Cardioprotective, Adaptogenic, Antihypoxic, Gastroprotective, Thermoprotective, Anabolic and Actotactic Activity and Method - Google Patents

Claims (2)

1. The use of the amount of phytoecdysteroids, including at least 80% of 20-hydroxyecdysone, 8-11% of 25S-inocosterone and 5.9-9.8% of α-ecdysone obtained from the aerial mass of the plant Serratula coronata L., collected in the growing phase or mass budding before flowering, as a cardioprotective, adaptogenic, antihypoxic, gastroprotective, thermoprotective, anabolic and actoprotective agents. 2. A method of manufacturing an agent representing the sum of phytoecdysteroids, including grinding the aerial mass of the plant Serratula coronata L., collected in the mass budding phase before flowering, extraction with water-ethanol mixture, separation of the extract, vacuum concentration, clarification by filtration through a sorbent, elution of phytoecdysteroids with sorbent, combining fractions, recrystallization of phytoecdysteroids and drying the finished product, characterized in that the plant material before extraction by pre-insisting work with boiling water with a specific flow rate of 2.6 l / kg of raw material for 20 minutes, or treat with steam at a temperature of 100 ° C with a specific flow rate of 0.03-0.09 kg / kg for 1-3 minutes, or mix with water with a specific flow rate of 3.5 l / kg and heated in a microwave oven to 100 ° C, the suspension of the extractant-raw material is treated with ultrasound with a specific power of 39 W / l for 5 min, sequentially extracted three times by infusion with 70-96% ethanol at process hydromodule 1: (9-15) for 4.5-36 h, the extract is separated by filtration and concentrated in vacuo at at a temperature of not more than 50 ° C, then the extract is clarified by filtration through a sorbent of the formula M _x O _y , (where M = Si, or Mg, or Al, x = 1 or 2, y = 1-3) or a mixture of these sorbents, clarified extract subjected to precipitation on alumina at a dry residue-sorbent ratio of 1: 9, followed by drying in vacuo, then the precipitate is placed in a flash chromatography column on top of alumina with a dispersion of 40-80 microns and phytoecdysteroids are eluted with an ethyl acetate-ethanol mixture with an ethanol concentration gradient of 0 % to 96%, recrystallization of phytoecdysteroids is pour twice from a mixture of ethyl acetate-ethanol and once from anhydrous ethanol with cooling to -72 ° C with intermediate filtration.