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RU2005120603A - Recombinant plasmid pSVH0106, which ensures the synthesis of Gl7ACA acylase in Escherichia coli cells, and the recombinant strain of Escherichia coli BL21 (DE3) / pSVH0106-PRODUCTOR GL7 - Google Patents

Recombinant plasmid pSVH0106, which ensures the synthesis of Gl7ACA acylase in Escherichia coli cells, and the recombinant strain of Escherichia coli BL21 (DE3) / pSVH0106-PRODUCTOR GL7 Download PDF

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Publication number
RU2005120603A
RU2005120603A RU2005120603/13A RU2005120603A RU2005120603A RU 2005120603 A RU2005120603 A RU 2005120603A RU 2005120603/13 A RU2005120603/13 A RU 2005120603/13A RU 2005120603 A RU2005120603 A RU 2005120603A RU 2005120603 A RU2005120603 A RU 2005120603A
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RU
Russia
Prior art keywords
psvh0106
escherichia coli
acylase
gl7aca
recombinant
Prior art date
Application number
RU2005120603/13A
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Russian (ru)
Other versions
RU2300566C2 (en
Inventor
Светлана Анатольевна Хатунцева (RU)
Светлана Анатольевна Хатунцева
Михаил Анатольевич Эльдаров (RU)
Михаил Анатольевич Эльдаров
Орхан Ахмед оглы Зейналов (RU)
Орхан Ахмед оглы Зейналов
бин Константин Георгиевич Скр (RU)
Константин Георгиевич Скрябин
Original Assignee
Центр "Биоинженери " РАН (RU)
Центр "Биоинженерия" Ран
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Priority to RU2005120603/13A priority Critical patent/RU2300566C2/en
Publication of RU2005120603A publication Critical patent/RU2005120603A/en
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Publication of RU2300566C2 publication Critical patent/RU2300566C2/en

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Claims (2)

1. Рекомбинантная плазмида pSVH0106, обеспечивающая синтез ацилазы глутарил-7-аминоцефалоспориновой кислоты (Gl7ACA-ацилазы) в клетках Escherichia coli, характеризующаяся тем, что содержит фрагмент ДНК с нуклеотидной последовательностью SEQ ID №6 от нуклеотида в положении 103 до нуклеотида в положении 2265, кодирующий полную аминокислотную последовательность Gl7ACA-ацилазы штамма Brevundimonas diminuta ВКМ В-1297, встроенный по сайтам рестрикции EcoRI и SacI в область полилинкера вектора рАСТ7, состоящего из фрагмента модифицированной в области полилинкера плазмиды рЕТ21d, содержащего промотор и терминатор РНК-полимеразы фага Т7, разделенные участком полилинкера, и фрагмента плазмиды рСYC117, содержащего репликон р15А и ген устойчивости к канамицину, объединенных между собой как показано на фиг 3.1. Recombinant plasmid pSVH0106, providing synthesis of glutaryl-7-aminocephalosporin acid acylase (Gl7ACA-acylase) in Escherichia coli cells, characterized in that it contains a DNA fragment with nucleotide sequence SEQ ID No 6 from nucleotide at position 103 to nucleotide at position 2265, encoding the complete amino acid sequence of the Gl7ACA acylase of the strain Brevundimonas diminuta VKM B-1297 inserted at the EcoRI and SacI restriction sites into the polylinker region of the pAST7 vector consisting of a fragment of the plasmid pET21d modified in the polylinker region containing zhaschego promoter and terminator of RNA polymerase of T7, separated by a portion of the polylinker, and a fragment rSYC117 plasmid containing r15A replicon and the kanamycin resistance gene, combined with each other as shown in Figure 3. 2. Рекомбинантный штамм Escherichia coli BL21(DE3)/pSVH0106-продуцент Gl7ACA-ацилазы.2. Recombinant strain of Escherichia coli BL21 (DE3) / pSVH0106-producer of Gl7ACA acylase.
RU2005120603/13A 2005-07-04 2005-07-04 RECOMBINANT PLASMID pSVH0106 PROVIDING SYNTHESIS OF Gl7ACA ACYLASE IN Escherichia coli CELLS, RECOMBINANT STRAIN OF Escherichia coli BL21(DE3)/pSVH0106 AS PRODUCER OF Gl7ACA ACYLASE RU2300566C2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU2005120603/13A RU2300566C2 (en) 2005-07-04 2005-07-04 RECOMBINANT PLASMID pSVH0106 PROVIDING SYNTHESIS OF Gl7ACA ACYLASE IN Escherichia coli CELLS, RECOMBINANT STRAIN OF Escherichia coli BL21(DE3)/pSVH0106 AS PRODUCER OF Gl7ACA ACYLASE

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU2005120603/13A RU2300566C2 (en) 2005-07-04 2005-07-04 RECOMBINANT PLASMID pSVH0106 PROVIDING SYNTHESIS OF Gl7ACA ACYLASE IN Escherichia coli CELLS, RECOMBINANT STRAIN OF Escherichia coli BL21(DE3)/pSVH0106 AS PRODUCER OF Gl7ACA ACYLASE

Publications (2)

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RU2005120603A true RU2005120603A (en) 2007-02-27
RU2300566C2 RU2300566C2 (en) 2007-06-10

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RU2005120603/13A RU2300566C2 (en) 2005-07-04 2005-07-04 RECOMBINANT PLASMID pSVH0106 PROVIDING SYNTHESIS OF Gl7ACA ACYLASE IN Escherichia coli CELLS, RECOMBINANT STRAIN OF Escherichia coli BL21(DE3)/pSVH0106 AS PRODUCER OF Gl7ACA ACYLASE

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2642323C2 (en) * 2016-06-09 2018-01-24 Федеральное государственное учреждение "Федеральный исследовательский центр "Фундаментальные основы биотехнологии" Российской академии наук" (ФИЦ Биотехнологии РАН) RECOMBINANT PRODUCER OF HUMAN OMEGA-AMIDASE Nit2 BASED ON ESCHERICHIA COLI

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Effective date: 20140705