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RU2003114571A - METHOD FOR PREDICTING THE COURSE OF ROTAVIRUS INFECTION IN CHILDREN - Google Patents

METHOD FOR PREDICTING THE COURSE OF ROTAVIRUS INFECTION IN CHILDREN

Info

Publication number
RU2003114571A
RU2003114571A RU2003114571/15A RU2003114571A RU2003114571A RU 2003114571 A RU2003114571 A RU 2003114571A RU 2003114571/15 A RU2003114571/15 A RU 2003114571/15A RU 2003114571 A RU2003114571 A RU 2003114571A RU 2003114571 A RU2003114571 A RU 2003114571A
Authority
RU
Russia
Prior art keywords
antigen
course
antibodies
infection
rel
Prior art date
Application number
RU2003114571/15A
Other languages
Russian (ru)
Other versions
RU2251695C2 (en
Inventor
Наталья Владимировна Сергеева
Олег Алексеевич Аксенов
Ольга Вячеславовна Тихомирова
Елена Александровна Мурина
Original Assignee
Научно-исследовательский институт детских инфекциий
Filing date
Publication date
Application filed by Научно-исследовательский институт детских инфекциий filed Critical Научно-исследовательский институт детских инфекциий
Priority to RU2003114571/15A priority Critical patent/RU2251695C2/en
Priority claimed from RU2003114571/15A external-priority patent/RU2251695C2/en
Publication of RU2003114571A publication Critical patent/RU2003114571A/en
Application granted granted Critical
Publication of RU2251695C2 publication Critical patent/RU2251695C2/en

Links

Claims (1)

Способ прогнозирования течения ротавирусной инфекции у детей, путем иммуноферментного определения наличия антигена в копрофильтратах больных, отличающийся тем, что используют один диагностикум, определяют одновременно ротавирусный антиген и антитела к ротавирусной инфекции, проводят сорбцию антична в копрофильтратах, соединяют надосадочную жидкость 50,0 мкл с пулом фекалий, содержащим ротавирусный антиген в количестве 0,400-0,500 отн. ед., выдерживают в термостате, затем вносят в панель с иммобилизированными антителами и по снижению уровня тест-антигена устанавливают наличие антител в копрофильтратах, а прогноз осуществляют по наличию антигена >1,3 отн.ед. и отсутствию антител -длительное течение инфекции, 6-8 дней, а при наличии антигена в той же концентрации (>1,3 отн.ед.) и антител в высокой концентрации 0,60-0,80 отн.ед. - течение инфекции 2-3 дня.A method for predicting the course of rotavirus infection in children by enzyme-linked immunosorbent assay for the presence of antigen in patient’s coprofiltrates, characterized in that they use one diagnosticum, rotavirus antigen and antibodies to rotavirus infection are determined simultaneously, sorption of antique in coprofiltrates is carried out, 50.0 μl of supernatant are combined with a pool feces containing rotavirus antigen in the amount of 0.400-0.500 rel. units, incubated in a thermostat, then introduced into the panel with immobilized antibodies and to reduce the level of test antigen, the presence of antibodies in the coprofiltrates is established, and the forecast is carried out by the presence of antigen> 1.3 rel. and the absence of antibodies, a prolonged course of infection, 6-8 days, and in the presence of antigen in the same concentration (> 1.3 rel. units) and antibodies in a high concentration of 0.60-0.80 rel. - the course of infection is 2-3 days.
RU2003114571/15A 2003-05-15 2003-05-15 Method for predicting the flow of erysipelas infection in children RU2251695C2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU2003114571/15A RU2251695C2 (en) 2003-05-15 2003-05-15 Method for predicting the flow of erysipelas infection in children

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU2003114571/15A RU2251695C2 (en) 2003-05-15 2003-05-15 Method for predicting the flow of erysipelas infection in children

Publications (2)

Publication Number Publication Date
RU2003114571A true RU2003114571A (en) 2004-11-20
RU2251695C2 RU2251695C2 (en) 2005-05-10

Family

ID=35747162

Family Applications (1)

Application Number Title Priority Date Filing Date
RU2003114571/15A RU2251695C2 (en) 2003-05-15 2003-05-15 Method for predicting the flow of erysipelas infection in children

Country Status (1)

Country Link
RU (1) RU2251695C2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2405411C2 (en) * 2009-01-11 2010-12-10 Государственное образовательное учреждение высшего профессионального образования "Красноярский государственный медицинский университет имени профессора В.Ф. Войно-Ясенецкого Министерства здравоохранения и социального развития Российской Федерации" Method for forecasting of rotavirus infection course in children
RU2665945C1 (en) * 2017-08-07 2018-09-05 Наталья Юрьевна Пшеничная Method for predicting the duration of the diarrhea and the selection of tactics of treatment

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