RU2082169C1 - Method of animal organism biostimulation - Google Patents

Abstract

FIELD: biology, immunology, biotechnology, agriculture. SUBSTANCE: method involves the use of the purified immunoglobulins isolated from serum blood of animals immunized with S-100 proteins by their intraperitoneal or intramuscular administration to animals. Method provides the stimulation of total viability, fertility, growth and weight of animals. EFFECT: increased productivity of production. 4 tbl

Description

 The invention relates to biology, immunology, biotechnology, agriculture, animal husbandry, livestock. The feasibility of introducing into agricultural practice new, economically viable ways to increase the productivity of domesticated animals does not require special justification. Potential success can be expected here, in particular, from developments in the field of genetic engineering. However, at present, such methods are at the very early stages of development, the roads are complex, time-consuming, unstable according to the results and, therefore, cannot yet be considered as suitable for quantitative use.

 At the same time, the development of molecular biology and immunology makes it possible today to propose and experimentally test much simpler and more affordable and at the same time potentially effective ways of influencing the growth, development and fertility of animals. Some of them may be economically feasible and suitable for practical use.

 The results of an experimental verification of one of this kind of theoretically substantiated hypotheses were the basis of the invention. Its essence was as follows.

 Data on the presence of the closest functional-metabolic relationships between the nervous and immune systems, as well as the presence of common antigens specific only for nerve and immunocompetent cells (Abramov V.V. Interaction of the nervous and immune systems, Novosibirsk, Nauka, 1988), determine the potential for simultaneous modulating (in particular, stimulating) effects on the functional state of both leading body homeostasis control systems. A similar effect can be exerted with the help of antibodies (antisera) of a specific specificity. At a certain intensity, depending on the concentration of introduced antisera, such an effect can positively affect the general condition of the body, contribute to its better adaptation to the environment and increase resistance to adverse factors.

 The invention was based on the results of an experimental verification of the assumption that the weak (stimulating) effects of antiserum on proteins of the S100 group (these proteins are synthesized by brain astrocytes and a number of cells of the immune system Carbone et al. Human Pathol. 1985, v.16, No. 11, 1157 -1164), introduced into the body of minks, can favorably affect the protective and homeostatic potentials of the animal organism and, therefore, reduce their mortality and increase fertility. Single intraperitoneal or intramuscular injections of adult mink fractions of immunoglobulins isolated from S100 antiserum prevented the death of adult animals in the autumn-winter period and promoted the appearance of a larger and more viable offspring.

 To date, antibodies or antisera (immunoglobulins) to S100 have been used exclusively for research purposes. Including, for studying the participation of S100 proteins in mechanisms of behavior (Yurkevich S.O. Poletaev A. B. Zh. Higher nervous activity. 1982, 31, 1, 79-85); for immunohistochemical studies (K. Shefasson et al. Brain Res. 1982, 234, 2, 309-317); to study the participation of S100 in the electrogenic functions of nerve cells (Filipovic D. et al. Acta Biol. Yugosl. 1987, C23, 1, 31-38), which does not allow their use as prototypes or analogues.

 As a prototype of the present invention, it is possible to consider the works analyzed in the Gelfand review (Clin. Immunol. A. Immunopathol. 1989, 53, 2, pt. 2, S1-S6), which essentially examines the adaptogenic properties of non-immune donor gamma globulins tested initially in animals, and effectively improving the general condition of patients with various forms of pathology (tumor, infectious, allergic diseases, toxicosis, etc.) as a result of the administration of large doses (up to 2 g / kg / day) of donor gamma globulin. The reasons for the positive effects and normalization of the overall homeostasis of the organism of mammals and humans under the influence of gamma globulin preparations remain unclear. It is assumed that the observed adaptive homeostatic effects may be based on the presence of antibodies of the desired orientation in the immunoglobulin preparations in the prepared drugs (the presence of antibodies to S100 proteins in the preparations cannot be ruled out). The high cost of gamma globulin preparations obtained from several thousand healthy donors limits the possibility of widespread use of such effects even in a clinic and makes it almost impossible to use such drugs in animal husbandry and animal farming. This disadvantage is eliminated by the proposed method of using preparations of enriched anti-S100 immunoglobulins as a bioadaptogen of general action, the main purpose of which is used on farm animals is to increase the efficiency and reduce the cost of achieving a bioadaptogenic effect.

 The method used to obtain the antigen for immunization (S100 proteins) is well known (see, for example, J. Neurochem. 1983, 40, 1, 145-152). Rabbits were immunized with S100 proteins as described previously (Belyaev S.V. et al. Biochemistry, 1982, v. 46, No. 12, 2193-2201).

 The method used to use the fraction of immunoglobulins from the serum of immunized S100 animals, that is, the use of passive anti-S100 immunization of animal recipients to stimulate their viability, fertility, growth and weight, have no analogues.

The method is as follows. Obtaining the drug BIOSTIMULATOR-PS, that is, fractions of immunoglobulins from the antiserum to S100 proteins, was carried out according to generally accepted methods. In 5 adult rabbits immunized with S100 proteins, 50-60 ml of blood was collected from the marginal ear vein, serum was separated from the formed elements, the immunoglobulin fraction was reprecipitated (enriched) from it in a semi-saturated solution of ammonium sulfate three times, the obtained fraction was dialyzed against 0.14 M sodium chloride solution, the protein concentration in the solution was determined spectrophotometrically, the preparation was sterilized by filtration through 0.2 μm Millipore filters, poured into ampoules and sealed. The drug was stored until use at 4 ^o C.

 The drug was tested on 2 groups of dark brown minks: on young six-month-old animals (n = 50), injecting them once per 10 mg / kg of the drug intraperitoneally; on sexually mature one and a half to two year old pregnant female minks (n = 15) with a single intraperitoneal injection of 10 mg / kg of the drug at the beginning of the last third of pregnancy.

 To confirm the absence of species-specific effects, the drug was also tested in small groups (10 pcs.) Of pregnant females of other animal foxes, arctic foxes and raccoons with a single intraperitoneal injection of the drug at the rate of 7 mg / kg weight.

 The experiments were carried out in the Kotkozersky breeding farm of the Olonets district of the Karelian Autonomous Soviet Socialist Republic.

 Evaluation of the effectiveness of the use of the drug biostimulant-PS.

The effect of the drug on the state of young minks was evaluated taking into account:
the death rate of animals during the autumn-winter period (from November to March) among animals that received a bioadaptogen, received an injection of non-immune gamma globulins, or did not receive drugs (passive control);
the ratio of animals retaining or gaining weight among these groups;
the amount of food consumed in the autumn-winter period among the named groups of animals (according to the balance of the average daily diet).

The effect of the drug administered to pregnant minks on the state of young minks was evaluated taking into account:
the average number of offspring in females of different groups;
the frequency of occurrence of weak litters;
the incidence of dyspeptic disorders requiring antibiotic therapy in puppies from the litter of females of different groups;
the death rate of puppies for the first 3 months of life from the litter of females of different groups.

 Example 1. The effect of the drug biostimulant-PS on the state of young minks is given in table 1.

 Example 2. The effect of the drug biostimulant-PS, administered to pregnant minks, on the state of young minks is given in table.2.

 The comparative effectiveness of the administration of the lowest (1 mg / kg) and high (10 mg / kg) doses of the biostimulant-PS preparation for changing the body weight of young minks during the autumn-winter period (November-March) as an integral indicator of the general physiological state of animals is given in Table. 3.

 The effect of the biostimulant-PS preparation, administered to pregnant females: on the number of litters in different species is given in Table 4.

 The use of the biostimulant-PS preparation (a single intraperitoneal injection of mink immunoglobulins isolated from the antiserum to S100 proteins) prevented the death of adult animals in the autumn-winter period and helped to improve the general physical condition of the animals.

 A single administration of the biostimulant-PS preparation to pregnant female minks, as well as other animals, made it possible to obtain a larger number of puppies and a more viable offspring.

 Obtaining the drug biostimulant-PS does not require complex equipment and expensive reagents.

 The species non-specificity of S100 proteins allows the use of antisera, antibodies or immunoglobulins to them (biostimulant-PS) to increase the vitality and productivity of different types of farm animals, which is why the biostimulant-PS preparation can be widely used in various sectors of animal husbandry and animal farming.

Claims (1)

 A method of biostimulating an animal’s body, comprising introducing immunoglobulin into the body, characterized in that a PS biostimulator containing immunoglobulin to S100 proteins is introduced into the body, the administration being carried out once at a dose of 10 mg / kg animal weight.

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