RU165361U1 - METHOD FOR MODELING INTRAABDOMINAL HYPERTENSION - Google Patents

Abstract

A method for modeling intra-abdominal hypertension by creating pressure in the abdominal cavity by injection of gas and controlling the pressure level in the abdominal cavity using an open urethral catheter, characterized in that a silicone balloon is installed in the abdominal cavity of the laboratory animal, hermetically connected to a tube with a diameter of 4-5 mm, the other end which is brought out through a separate puncture along the left lateral surface of the abdominal wall, then a silicone tube is inserted into the bladder through a puncture along the front surface 3-4 mm in diameter, the other end of which is fed through a separate puncture of the right side surface of the abdomen, and the bladder silicone tube attached via purse-string suture, and then laparotomy wound sutured tightly.

Description

The utility model relates to experimental medicine and can be used to study the effect of increased intra-abdominal pressure on the organs and systems of a laboratory animal.

As a prototype, a method for modeling intra-abdominal hypertension was selected (Zabelin M.V. Syndrome of intra-abdominal hypertension in emergency abdominal surgery: abstract of thesis for the degree of candidate of medical sciences. - M., 2010. - 22 p.) consists in increasing intra-abdominal pressure (VBD) in laboratory animals as follows: a rigid corset is installed on the abdomen, which is a bandage made of dense cotton fabric with lacing; lacing is tightened, and in the abdominal cavity, through a catheter, using an infusomat, a carboxyperitoneum is created to the required level of intra-abdominal pressure, and the IOP level is monitored through a catheter installed in the bladder according to method I.

Signs that coincide with the essential features of the claimed method are: the creation of pressure in the abdominal cavity by gas injection

and with control of the level of pressure in the abdominal cavity by the method of an open urethral catheter.

The reasons that impede the achievement of the expected technical result are: the complexity, the need for special equipment and insufficient adaptation for a long experiment.

The basis of the invention is the task of improving the prototype method by optimizing the simulation of increased VBD in laboratory animals.

The problem is solved in that in the method for modeling intra-abdominal hypertension by creating pressure in the abdominal cavity by injection of gas and controlling the level of pressure in the abdominal cavity by the method of an open urethral catheter, according to a utility model, a silicone balloon is installed in the abdominal cavity of a laboratory animal, hermetically connected to a tube with a diameter of 4 -5 mm, the other end of which is led out through a separate puncture along the left lateral surface of the abdominal wall, then into the bladder through a puncture in front of s surface is introduced silicone tube 3-4 mm in diameter, the other end of which is fed through a separate puncture of the right side surface of the abdomen, and the bladder silicone tube attached via purse-string suture, and then laparotomy wound sutured tightly.

The method is illustrated in the drawing, which shows a simulation scheme for increased intra-abdominal pressure in laboratory animals, where 1 is a silicone balloon, 2 is a tube, 3 is a puncture of the abdominal wall, 4 is the left side surface of the abdominal wall, 5 is the bladder, 6 is the front wall of the urinary a bubble, 7 - a silicone tube, 8 - a puncture along the right lateral surface of the abdomen, 9 - a purse string suture, 10 - a Janet syringe, an 11-centimeter ruler.

The method is as follows.

Under general anesthesia, the animal undergoes a median laparotomy. A silicone balloon 1 is installed in the abdominal cavity (subphrenic space), hermetically connected to a tube 2 with a diameter of 5 mm, the opposite end of the tube 2 is brought out through a separate puncture 3 along the left side surface of the abdominal wall 4. Then into the bladder 5 through a puncture along the front surface 6 a silicone tube 7 is inserted with a diameter of 3 mm, the opposite end of which is led out through a separate puncture 8 along the right lateral surface of the abdomen. A silicone tube 7 is attached to the bladder 5 with a purse string suture 9. After which the laparotomy wound is sutured tightly. Subsequently, increased VBD is modeled by injecting air into a silicone balloon 1 through a tube 2 using a Janet syringe 10. The level of VBD is controlled by the method of an open urethral catheter through a silicone tube 7 using a centimeter ruler 11.

The proposed method for modeling intra-abdominal hypertension in laboratory animals is technically simple to implement, does not require additional equipment, and can be used with a long experiment.

Using this technique, we simulated intra-abdominal hypertension in 28 laboratory animals (rabbits), which allowed us to study the isolated effect of increased IOP on the organs and systems of the animal’s body.

Claims (1)

A method for modeling intra-abdominal hypertension by creating pressure in the abdominal cavity by injection of gas and controlling the pressure level in the abdominal cavity using an open urethral catheter, characterized in that a silicone balloon is installed in the abdominal cavity of the laboratory animal, hermetically connected to a tube with a diameter of 4-5 mm, the other end which is brought out through a separate puncture along the left lateral surface of the abdominal wall, then a silicone tube is inserted into the bladder through a puncture along the front surface 3-4 mm in diameter, the other end of which is fed through a separate puncture of the right side surface of the abdomen, and the bladder silicone tube attached via purse-string suture, and then laparotomy wound sutured tightly.

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