RO93651B - Process for obtaining recombinated lymphotoxin - Google Patents
Process for obtaining recombinated lymphotoxinInfo
- Publication number
- RO93651B RO93651B RO118983A RO11898385A RO93651B RO 93651 B RO93651 B RO 93651B RO 118983 A RO118983 A RO 118983A RO 11898385 A RO11898385 A RO 11898385A RO 93651 B RO93651 B RO 93651B
- Authority
- RO
- Romania
- Prior art keywords
- lymphotoxin
- dna
- encoding
- obtaining
- recombinated
- Prior art date
Links
- 102000004083 Lymphotoxin-alpha Human genes 0.000 title abstract 5
- 108090000542 Lymphotoxin-alpha Proteins 0.000 title abstract 5
- 238000000034 method Methods 0.000 title abstract 3
- 206010028980 Neoplasm Diseases 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 210000004962 mammalian cell Anatomy 0.000 abstract 1
- 244000005700 microbiome Species 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 230000009466 transformation Effects 0.000 abstract 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Inventia se refera la un procedeu de obtinere a limfotoxinei recombinate, care cuprinde construirea cu ajutorul unui DNA care codifica limfotoxina, ales dintre DNA liber de secventele netranslate, DNA liber de DNA care codifica alte proteine ale organismului din care provine, sau DNA lipsit de secventa nucleotidica de DNA natural sau RNA care codifica limfotoxina, capabil de hibridare pentru obtinerea de DNA care codifica limfotoxina, a unui vector replicabil, transformarea cu ajutorul acestuia a unei celule de microorganism sau de mamifer, cultivarea celulei transformate pentru obtinerea limfotoxinei si recuperarea acesteia din cultura în mod cunoscut. Procedeul, conform inventiei, permite obtinerea într-un mod avantajos a unei cantitati utile de limfotoxina, utilizabila în tratamentul tumorilor.The invention relates to a process for obtaining recombinant lymphotoxin which comprises constructing with a DNA encoding lymphotoxin selected from free DNA from untranslated sequences, DNA free DNA encoding other proteins of the organism from which it originates or DNA lacking sequence nucleotide of natural DNA or lymphotoxin-encoding RNA capable of hybridizing to obtain lymphotoxin-encoding DNA, a replicable vector, transformation thereof with a microorganism or mammalian cell, culturing the transformed cell to obtain lymphotoxin and recovering it from the culture in a known way. The process according to the invention allows to advantageously obtain a useful amount of lymphotoxin, useful in the treatment of tumors.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US73231285A | 1985-05-09 | 1985-05-09 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RO93651A RO93651A (en) | 1989-01-30 |
| RO93651B true RO93651B (en) | 1989-01-31 |
Family
ID=24943042
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RO118983A RO93651B (en) | 1985-05-09 | 1985-05-30 | Process for obtaining recombinated lymphotoxin |
Country Status (1)
| Country | Link |
|---|---|
| RO (1) | RO93651B (en) |
-
1985
- 1985-05-30 RO RO118983A patent/RO93651B/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| RO93651A (en) | 1989-01-30 |
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