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LT3456846T - Nukleorūgšties unikalios sekos įvedimo sistemos ir būdai - Google Patents

Nukleorūgšties unikalios sekos įvedimo sistemos ir būdai

Info

Publication number
LT3456846T
LT3456846T LTEP18201501.6T LT18201501T LT3456846T LT 3456846 T LT3456846 T LT 3456846T LT 18201501 T LT18201501 T LT 18201501T LT 3456846 T LT3456846 T LT 3456846T
Authority
LT
Lithuania
Prior art keywords
systems
methods
nucleic acid
barcoding nucleic
barcoding
Prior art date
Application number
LTEP18201501.6T
Other languages
English (en)
Inventor
David A. Weitz
Allon Moshe Klein
Ilke Akartuna
Linas Mazutis
Marc W. Kirschner
Original Assignee
President And Fellows Of Harvard College
Vilnius University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by President And Fellows Of Harvard College, Vilnius University filed Critical President And Fellows Of Harvard College
Publication of LT3456846T publication Critical patent/LT3456846T/lt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
LTEP18201501.6T 2014-04-21 2015-04-17 Nukleorūgšties unikalios sekos įvedimo sistemos ir būdai LT3456846T (lt)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201461982001P 2014-04-21 2014-04-21
US201462065348P 2014-10-17 2014-10-17
US201462066188P 2014-10-20 2014-10-20
US201462072944P 2014-10-30 2014-10-30

Publications (1)

Publication Number Publication Date
LT3456846T true LT3456846T (lt) 2022-09-12

Family

ID=54333034

Family Applications (3)

Application Number Title Priority Date Filing Date
LTEP17198030.3T LT3299469T (lt) 2014-04-21 2015-04-17 Sistemos ir būdai, skirti nukleorūgšties identifikavimo žymės įvedimui
LTEP15783629.7T LT3134536T (lt) 2014-04-21 2015-04-17 Sistemos ir būdai, skirti nukleorūgščių brūkšniniam kodavimui
LTEP18201501.6T LT3456846T (lt) 2014-04-21 2015-04-17 Nukleorūgšties unikalios sekos įvedimo sistemos ir būdai

Family Applications Before (2)

Application Number Title Priority Date Filing Date
LTEP17198030.3T LT3299469T (lt) 2014-04-21 2015-04-17 Sistemos ir būdai, skirti nukleorūgšties identifikavimo žymės įvedimui
LTEP15783629.7T LT3134536T (lt) 2014-04-21 2015-04-17 Sistemos ir būdai, skirti nukleorūgščių brūkšniniam kodavimui

Country Status (8)

Country Link
EP (3) EP3134536B1 (lt)
JP (1) JP6853667B2 (lt)
CN (1) CN107075543B (lt)
AU (1) AU2015250034B2 (lt)
CA (1) CA2946144C (lt)
DK (3) DK3299469T3 (lt)
LT (3) LT3299469T (lt)
WO (1) WO2015164212A1 (lt)

Families Citing this family (166)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1663497B2 (en) 2003-09-05 2020-03-25 Stokes Bio Limited A microfluidic analysis system
US9597644B2 (en) * 2003-09-05 2017-03-21 Stokes Bio Limited Methods for culturing and analyzing cells
EP1984738A2 (en) 2006-01-11 2008-10-29 Raindance Technologies, Inc. Microfluidic devices and methods of use in the formation and control of nanoreactors
WO2007091228A1 (en) 2006-02-07 2007-08-16 Stokes Bio Limited A liquid bridge and system
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
WO2007133710A2 (en) 2006-05-11 2007-11-22 Raindance Technologies, Inc. Microfluidic devices and methods of use thereof
US8772046B2 (en) 2007-02-06 2014-07-08 Brandeis University Manipulation of fluids and reactions in microfluidic systems
WO2008130623A1 (en) 2007-04-19 2008-10-30 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
WO2010009365A1 (en) 2008-07-18 2010-01-21 Raindance Technologies, Inc. Droplet libraries
US12038438B2 (en) 2008-07-18 2024-07-16 Bio-Rad Laboratories, Inc. Enzyme quantification
US8528589B2 (en) 2009-03-23 2013-09-10 Raindance Technologies, Inc. Manipulation of microfluidic droplets
EP4484577A3 (en) 2010-02-12 2025-03-26 Bio-Rad Laboratories, Inc. Digital analyte analysis
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
US9150852B2 (en) 2011-02-18 2015-10-06 Raindance Technologies, Inc. Compositions and methods for molecular labeling
EP3709018A1 (en) 2011-06-02 2020-09-16 Bio-Rad Laboratories, Inc. Microfluidic apparatus for identifying components of a chemical reaction
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
EP3495503A1 (en) 2012-03-05 2019-06-12 President and Fellows of Harvard College Systems and methods for epigenetic sequencing
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) * 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP4397767A3 (en) 2012-08-14 2024-07-31 10X Genomics, Inc. Microcapsule compositions and methods
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2014093676A1 (en) 2012-12-14 2014-06-19 10X Technologies, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
CN108753766A (zh) 2013-02-08 2018-11-06 10X基因组学有限公司 多核苷酸条形码生成
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
CA2943624A1 (en) 2014-04-10 2015-10-15 10X Genomics, Inc. Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
US20150298091A1 (en) 2014-04-21 2015-10-22 President And Fellows Of Harvard College Systems and methods for barcoding nucleic acids
US11155809B2 (en) 2014-06-24 2021-10-26 Bio-Rad Laboratories, Inc. Digital PCR barcoding
EP3161160B1 (en) 2014-06-26 2021-10-13 10X Genomics, Inc. Methods of analyzing nucleic acids from individual cells or cell populations
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
AU2015279617A1 (en) 2014-06-26 2017-01-12 10X Genomics, Inc. Analysis of nucleic acid sequences
EP4105337A1 (en) * 2014-09-09 2022-12-21 The Broad Institute, Inc. A droplet-based method and apparatus for composite single-cell nucleic acid analysis
MX2017005267A (es) 2014-10-29 2017-07-26 10X Genomics Inc Metodos y composiciones para la secuenciacion de acidos nucleicos seleccionados como diana.
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
CN107427808B (zh) 2015-01-12 2020-10-23 10X基因组学有限公司 用于制备核酸测序文库的方法和系统以及用其制备的文库
WO2016126871A2 (en) 2015-02-04 2016-08-11 The Regents Of The University Of California Sequencing of nucleic acids via barcoding in discrete entities
US10697000B2 (en) * 2015-02-24 2020-06-30 10X Genomics, Inc. Partition processing methods and systems
AU2016222719B2 (en) 2015-02-24 2022-03-31 10X Genomics, Inc. Methods for targeted nucleic acid sequence coverage
CN107614096A (zh) 2015-03-13 2018-01-19 哈佛学院院长及董事 使用扩增测定细胞
CN107614684A (zh) 2015-04-17 2018-01-19 哈佛学院院长及董事 用于基因测序和其它应用的条形编码系统及方法
SG11201803646YA (en) * 2015-11-04 2018-05-30 Atreca Inc Combinatorial sets of nucleic acid barcodes for analysis of nucleic acids associated with single cells
WO2017083375A1 (en) * 2015-11-10 2017-05-18 Illumina, Inc. Inertial droplet generation and particle encapsulation
US11371094B2 (en) 2015-11-19 2022-06-28 10X Genomics, Inc. Systems and methods for nucleic acid processing using degenerate nucleotides
KR20240161696A (ko) 2015-12-04 2024-11-12 10엑스 제노믹스, 인크. 핵산 분석을 위한 방법 및 조성물
WO2017100350A1 (en) * 2015-12-07 2017-06-15 Raindance Technologies, Inc. Multiplexing in partitions using microparticles
CN108779491B (zh) 2016-02-11 2021-03-09 10X基因组学有限公司 用于全基因组序列数据的从头组装的系统、方法和介质
US10633648B2 (en) 2016-02-12 2020-04-28 University Of Washington Combinatorial photo-controlled spatial sequencing and labeling
KR102379048B1 (ko) 2016-05-02 2022-03-28 엔코디아, 인코포레이티드 암호화 핵산을 사용한 거대분자 분석
WO2017197343A2 (en) 2016-05-12 2017-11-16 10X Genomics, Inc. Microfluidic on-chip filters
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
US20190329209A1 (en) * 2016-06-14 2019-10-31 Mission Bio, Inc. Methods and compositions for emulsification of solid supports in deformable beads
EP3260201A1 (en) 2016-06-21 2017-12-27 Hifibio Microfluidic filtration unit and related microfluidic system
LT3263715T (lt) 2016-06-28 2020-06-25 Hifibio Pavienių ląstelių transkriptomo analizės būdas
EP3545104B1 (en) * 2016-11-23 2021-02-24 Université de Strasbourg Tandem barcoding of target molecules for their absolute quantification at single-entity resolution
CA3047328A1 (en) * 2016-12-21 2018-06-28 The Regents Of The University Of California Single cell genomic sequencing using hydrogel based droplets
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US12264411B2 (en) 2017-01-30 2025-04-01 10X Genomics, Inc. Methods and systems for analysis
EP4029939B1 (en) 2017-01-30 2023-06-28 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
US10995333B2 (en) 2017-02-06 2021-05-04 10X Genomics, Inc. Systems and methods for nucleic acid preparation
EP4218738B1 (en) 2017-02-24 2024-10-16 The Regents of The University of California Particle-drop structures and methods for making and using the same
JP6916309B2 (ja) 2017-03-13 2021-08-11 ギガジェン，インコーポレイティッド 単一細胞の大規模並行コンビナトリアル分析のためのシステム及び方法
LT3375889T (lt) 2017-03-17 2020-06-25 Hifibio Sas Vienos ląstelės analizė
CN110678558B (zh) * 2017-05-02 2023-06-02 国立大学法人东京大学 整体性检测单个细胞的非破坏性测量信息和基因组相关信息的方法
US11072816B2 (en) 2017-05-03 2021-07-27 The Broad Institute, Inc. Single-cell proteomic assay using aptamers
WO2018213643A1 (en) 2017-05-18 2018-11-22 10X Genomics, Inc. Methods and systems for sorting droplets and beads
US10544413B2 (en) 2017-05-18 2020-01-28 10X Genomics, Inc. Methods and systems for sorting droplets and beads
WO2018218222A1 (en) 2017-05-26 2018-11-29 Goldfless Stephen Jacob High-throughput polynucleotide library sequencing and transcriptome analysis
EP3445876B1 (en) 2017-05-26 2023-07-05 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
US10844372B2 (en) 2017-05-26 2020-11-24 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
US20210246488A1 (en) * 2017-08-14 2021-08-12 Mission Bio, Inc. Method and compositions for evaluating emulsion uniformity
US10549279B2 (en) 2017-08-22 2020-02-04 10X Genomics, Inc. Devices having a plurality of droplet formation regions
EP3679370A1 (en) 2017-09-07 2020-07-15 Juno Therapeutics, Inc. Methods of identifying cellular attributes related to outcomes associated with cell therapy
RU2756306C1 (ru) * 2017-09-22 2021-09-29 федеральное государственное автономное образовательное учреждение высшего образования "Тюменский государственный университет" Система и способ для анализа фенотипа и полинуклеотидного секвенирования биологических частиц с использованием детерминированного штрихкодирования
WO2019060830A1 (en) * 2017-09-25 2019-03-28 Plexium, Inc. CHEMICAL LIBRARIES CODED BY OLIGONUCLEOTIDES
ES2998086T3 (en) * 2017-09-29 2025-02-19 Univ California Method of generating monodisperse emulsions
US10837047B2 (en) 2017-10-04 2020-11-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
JP7208230B2 (ja) * 2017-10-09 2023-01-18 プソマーゲン， インコーポレイテッド 核酸配列を特性決定するための単一分子シーケンシング及び固有の分子識別子
WO2019083852A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. MICROFLUIDIC CHANNEL NETWORKS FOR PARTITIONING
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS
WO2019084165A1 (en) 2017-10-27 2019-05-02 10X Genomics, Inc. METHODS AND SYSTEMS FOR SAMPLE PREPARATION AND ANALYSIS
CA3081441C (en) 2017-10-31 2023-08-29 Encodia, Inc. Kits for analysis using nucleic acid encoding and/or label
SG11201913654QA (en) 2017-11-15 2020-01-30 10X Genomics Inc Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
US11788120B2 (en) 2017-11-27 2023-10-17 The Trustees Of Columbia University In The City Of New York RNA printing and sequencing devices, methods, and systems
WO2019108851A1 (en) 2017-11-30 2019-06-06 10X Genomics, Inc. Systems and methods for nucleic acid preparation and analysis
WO2019118355A1 (en) 2017-12-12 2019-06-20 10X Genomics, Inc. Systems and methods for single cell processing
CN111712579B (zh) 2017-12-22 2024-10-15 10X基因组学有限公司 用于处理来自一个或多个细胞的核酸分子的系统和方法
EP3752832A1 (en) * 2018-02-12 2020-12-23 10X Genomics, Inc. Methods characterizing multiple analytes from individual cells or cell populations
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
WO2019169028A1 (en) 2018-02-28 2019-09-06 10X Genomics, Inc. Transcriptome sequencing through random ligation
US11841371B2 (en) 2018-03-13 2023-12-12 The Broad Institute, Inc. Proteomics and spatial patterning using antenna networks
WO2019191321A1 (en) * 2018-03-28 2019-10-03 10X Genomics, Inc. Nucleic acid enrichment within partitions
SG11202009889VA (en) 2018-04-06 2020-11-27 10X Genomics Inc Systems and methods for quality control in single cell processing
WO2019217758A1 (en) 2018-05-10 2019-11-14 10X Genomics, Inc. Methods and systems for molecular library generation
US11414701B2 (en) 2018-05-24 2022-08-16 The Broad Institute, Inc. Multimodal readouts for quantifying and sequencing nucleic acids in single cells
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
US11703427B2 (en) 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing
EP3821032A1 (en) * 2018-07-13 2021-05-19 HiFiBiO SAS Use of droplet single cell epigenome profiling for patient stratification
US20210310063A1 (en) * 2018-07-13 2021-10-07 Hifibio Sas Use of droplet single cell epigenome profiling for patient stratification
US12188014B1 (en) 2018-07-25 2025-01-07 10X Genomics, Inc. Compositions and methods for nucleic acid processing using blocking agents
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
EP3830289A1 (en) 2018-08-03 2021-06-09 10X Genomics, Inc. Methods and systems to minimize barcode exchange
WO2020041148A1 (en) 2018-08-20 2020-02-27 10X Genomics, Inc. Methods and systems for detection of protein-dna interactions using proximity ligation
US12065688B2 (en) 2018-08-20 2024-08-20 10X Genomics, Inc. Compositions and methods for cellular processing
WO2020131182A2 (en) * 2018-09-26 2020-06-25 The University Of North Carolina At Chapel Hill Compounds, compositions, and methods for improving assays
FI3856404T3 (fi) 2018-09-28 2024-10-29 Fluent Biosciences Inc Kohteen sieppaus monodispergoituihin pisaroihin
JP7279885B2 (ja) * 2018-11-07 2023-05-23 国立大学法人 東京大学 １種以上の被検物質と共存した細胞のゲノム関連情報を検出する方法
US11512356B2 (en) * 2018-11-08 2022-11-29 Tokitae Llc Systems and methods for particle multiplexing in droplets
US11459607B1 (en) 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
AU2019398307A1 (en) 2018-12-13 2021-06-10 Dna Script Direct oligonucleotide synthesis on cells and biomolecules
CN109851711B (zh) * 2018-12-29 2020-09-25 苏州绘真生物科技有限公司 可溶性水凝胶微球及其制备方法与在单细胞检测中的应用
US12169198B2 (en) 2019-01-08 2024-12-17 10X Genomics, Inc. Systems and methods for sample analysis
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
US12275993B2 (en) 2019-02-12 2025-04-15 10X Genomics, Inc. Analysis of nucleic acid sequences
US12305239B2 (en) 2019-02-12 2025-05-20 10X Genomics, Inc. Analysis of nucleic acid sequences
WO2020167862A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transfer of reagents between droplets
US11851683B1 (en) 2019-02-12 2023-12-26 10X Genomics, Inc. Methods and systems for selective analysis of cellular samples
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
SG11202108788TA (en) 2019-02-12 2021-09-29 10X Genomics Inc Methods for processing nucleic acid molecules
WO2020167866A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transposon loading
US11655499B1 (en) 2019-02-25 2023-05-23 10X Genomics, Inc. Detection of sequence elements in nucleic acid molecules
WO2020185791A1 (en) 2019-03-11 2020-09-17 10X Genomics, Inc. Systems and methods for processing optically tagged beads
EP3938539A4 (en) * 2019-03-12 2022-12-14 Universal Sequencing Technology METHOD OF INTRACELLULAR SINGLE CELL TRACTION AND ITS APPLICATIONS
WO2020206285A1 (en) * 2019-04-05 2020-10-08 Board Of Regents, The University Of Texas System Methods and applications for cell barcoding
WO2020223000A1 (en) 2019-04-30 2020-11-05 Encodia, Inc. Methods for preparing analytes and related kits
AU2020280104A1 (en) 2019-05-22 2022-01-20 Mission Bio, Inc. Method and apparatus for simultaneous targeted sequencing of DNA, RNA and protein
US11667954B2 (en) 2019-07-01 2023-06-06 Mission Bio, Inc. Method and apparatus to normalize quantitative readouts in single-cell experiments
EP4025709A1 (en) * 2019-09-06 2022-07-13 10X Genomics, Inc. Systems and methods for barcoding cells and cell beads
US12235262B1 (en) 2019-09-09 2025-02-25 10X Genomics, Inc. Methods and systems for single cell protein analysis
CA3157359A1 (en) 2019-10-10 2021-04-15 1859, Inc. Methods and systems for microfluidic screening
GB201918340D0 (en) * 2019-12-12 2020-01-29 Cambridge Entpr Ltd Spatial barcoding
CN111172257A (zh) * 2020-01-16 2020-05-19 南方科技大学 一种带编码的凝胶微粒及其制备方法和应用
EP4095256A4 (en) * 2020-01-23 2023-03-15 DGI Tech (Qing Dao) Co., Limited Droplet microfluidics-based single cell sequencing and applications
US12449419B1 (en) 2020-02-12 2025-10-21 10X Genomics, Inc. Methods for detecting binding of peptide-MHC monomers to T cells
CA3153296A1 (en) * 2020-03-24 2021-09-30 Alex Chenchik Single cell genetic analysis
US11851700B1 (en) 2020-05-13 2023-12-26 10X Genomics, Inc. Methods, kits, and compositions for processing extracellular molecules
US12480158B1 (en) 2020-11-05 2025-11-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US12084715B1 (en) 2020-11-05 2024-09-10 10X Genomics, Inc. Methods and systems for reducing artifactual antisense products
EP4267301A1 (en) * 2020-12-22 2023-11-01 HiFiBiO SAS Microfluidic methods and systems
JP2024502618A (ja) 2021-01-08 2024-01-22 セラノーム， インコーポレイテッド 生体試料を分析するためのデバイスおよび方法
US12398262B1 (en) 2021-01-22 2025-08-26 10X Genomics, Inc. Triblock copolymer-based cell stabilization and fixation system and methods of use thereof
EP4298244A1 (en) 2021-02-23 2024-01-03 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins
US20250034640A1 (en) * 2021-04-20 2025-01-30 Simsen Diagnostics Ab Compositions and methods for cell-free nucleic acid isolation
CN113215233A (zh) * 2021-05-15 2021-08-06 墨卓生物科技(浙江)有限公司 一种用于单细胞测序的具有特定寡核苷酸序列的微球
WO2022256612A1 (en) * 2021-06-04 2022-12-08 Enumerix, Inc. Compositions, methods, and systems for single cell barcoding and sequencing
EP4351788A4 (en) 2021-06-04 2025-04-09 Enumerix, Inc. COMPOSITIONS, METHODS AND SYSTEMS FOR SINGLE-CELL ENCODING AND SEQUENCING
CN113584598A (zh) * 2021-06-17 2021-11-02 南方科技大学 一种单细胞文库的构建方法
CA3223500A1 (en) * 2021-06-24 2022-12-29 Andrea MANZO Methods and compositions for combinatorial indexing of bead-based nucleic acids
WO2023025784A1 (en) * 2021-08-24 2023-03-02 Ecole Polytechnique Federale De Lausanne (Epfl) Optimised set of oligonucleotides for bulk rna barcoding and sequencing
CN113889192B (zh) * 2021-09-29 2024-02-27 西安热工研究院有限公司 一种基于深层降噪自编码器的单细胞RNA-seq数据聚类方法
EP4437128A1 (en) * 2021-12-01 2024-10-02 Vilnius University Methods for processing and barcoding nucleic acids
CN114177960B (zh) * 2021-12-15 2023-04-28 天津大学 基于超高频体声波谐振器的微液滴操纵方法以及装置
WO2023139272A1 (en) * 2022-01-24 2023-07-27 Scipio Bioscience Gelation device with piston
EP4536854A1 (en) * 2022-06-07 2025-04-16 Alithea Genomics SA Optimised set of oligonucleotides for bulk rna barcoding and sequencing
WO2024011226A1 (en) * 2022-07-07 2024-01-11 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Cell-barcode recorder devices and methods
EP4331724A1 (en) * 2022-08-29 2024-03-06 Institut Curie Method of extracting a bead from a droplet
EP4435114A1 (en) 2023-03-24 2024-09-25 Miltenyi Biotec B.V. & Co. KG Syntheis of highly multiplexed oligo-barcoded fluorescent beads for single cell seq applications

Family Cites Families (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006507921A (ja) 2002-06-28 2006-03-09 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 流体分散のための方法および装置
EP3023140B1 (en) 2003-04-10 2019-10-09 President and Fellows of Harvard College Formation and control of fluidic species
EP2662136A3 (en) 2003-08-27 2013-12-25 President and Fellows of Harvard College Method for handling and mixing droplets
WO2006096571A2 (en) 2005-03-04 2006-09-14 President And Fellows Of Harvard College Method and apparatus for forming multiple emulsions
JP2006349060A (ja) 2005-06-16 2006-12-28 Ntn Corp ボールねじ
ATE484335T1 (de) 2006-01-27 2010-10-15 Harvard College Koaleszenz fluider tröpfchen
EP2129803A4 (en) * 2007-02-27 2010-11-03 Univ Northwestern APPLICATION OF MOLECULES TO NANOPARTICLES
WO2008109176A2 (en) 2007-03-07 2008-09-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
US20090181375A1 (en) * 2008-01-11 2009-07-16 Peter Brian J Method for detection of nucleic acid barcodes
US20110218123A1 (en) 2008-09-19 2011-09-08 President And Fellows Of Harvard College Creation of libraries of droplets and related species
EP3290531B1 (en) 2008-12-19 2019-07-24 President and Fellows of Harvard College Particle-assisted nucleic acid sequencing
JP5457222B2 (ja) * 2009-02-25 2014-04-02 エフ．ホフマン−ラ ロシュ アーゲー 小型化ハイスループット核酸分析
SG174617A1 (en) * 2009-04-02 2011-10-28 Fluidigm Corp Multi-primer amplification method for barcoding of target nucleic acids
CN102483424B (zh) 2009-06-26 2014-11-05 哈佛学院院长等 用于流体操作的方法以及微流体装置
CA2778816C (en) 2009-10-27 2018-07-31 President And Fellows Of Harvard College Droplet creation techniques
WO2012003330A2 (en) * 2010-06-30 2012-01-05 Stratos Genomics, Inc Multiplexed identification of nucleic acid sequences
WO2012048341A1 (en) * 2010-10-08 2012-04-12 President And Fellows Of Harvard College High-throughput single cell barcoding
US20140057799A1 (en) * 2010-12-16 2014-02-27 Gigagen System and Methods for Massively Parallel Analysis of Nucleic Acids in Single Cells
US9150852B2 (en) * 2011-02-18 2015-10-06 Raindance Technologies, Inc. Compositions and methods for molecular labeling
JP5822929B2 (ja) * 2011-07-19 2015-11-25 株式会社日立ハイテクノロジーズ 核酸分析装置
WO2013116698A2 (en) * 2012-02-02 2013-08-08 Invenra, Inc. High throughput screen for biologically active polypeptides
EP3495503A1 (en) * 2012-03-05 2019-06-12 President and Fellows of Harvard College Systems and methods for epigenetic sequencing
US9708654B2 (en) * 2012-06-15 2017-07-18 Board Of Regents, The University Of Texas System High throughput sequencing of multiple transcripts
WO2014047561A1 (en) * 2012-09-21 2014-03-27 The Broad Institute Inc. Compositions and methods for labeling of agents
PT3089822T (pt) * 2013-12-30 2022-05-06 Atreca Inc Análise de ácidos nucleicos associados com células isoladas usando códigos de barras de ácido nucleico
US11155809B2 (en) * 2014-06-24 2021-10-26 Bio-Rad Laboratories, Inc. Digital PCR barcoding

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