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KR940703926A - Dna 프로브 검정을 위한 개선된 전기화학발광 라벨(improved electrochemiluminescent label for dna probe assays) - Google Patents

Dna 프로브 검정을 위한 개선된 전기화학발광 라벨(improved electrochemiluminescent label for dna probe assays)

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KR940703926A
KR940703926A KR1019940702034A KR19940702034A KR940703926A KR 940703926 A KR940703926 A KR 940703926A KR 1019940702034 A KR1019940702034 A KR 1019940702034A KR 19940702034 A KR19940702034 A KR 19940702034A KR 940703926 A KR940703926 A KR 940703926A
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아아르 구디반데 사탸나라야나
에이취 켄튼 존
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리차아드 제이 마세이
아이젠 인코포레이팃드
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Abstract

본 발명은 포스포라미디트 화학약품을 사용하는 신규한 올리고뉴클레오티드용 전기화학발광 (ECL)라벨에 관한 것이다.

Description

DNA 프로브 검정을 위한 개선된 전기화학발광 라벨(IMPROVED ELECTROCHEMILUMINESCENT LABEL FOR DNA PROBE ASSAYS)
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (14)

  1. 하기 식의 화합물 :
    이때 M은 Ru, Os 및 Re로 구성된 군으로부터 선택되고 ; u·t=v,u는 1 또는 2이고 t는 1 또는 2이고 ; L1, L2및 L3는 동일하거나 상이하고 각각은
    이고, 이때 R1,R2,R3,R4,R5및 R6는 동일하거나 상이하고 각각은 H, 1-5 탄소 원자의 알킬기,
    R1,R2,R3,R4,R5 및 R6중 적어도 하나는
    n은 1-20 범위의 정수이고 ; X는 O,S,SO2,COO, 및 CONH로 구성된 군으로부터 선택되고 ; a 및 b는또는 모르폴리노이고, 단 a 및 b는 동일하지 않고 ; Z는 M과 관련된 반대 이온이고 이는 부가적으로, 리간드 L1,L2및 L3가 상기 화합물이 전기화학발광을 할 수 있도록 선택됨을 조건으로 한다.
  2. 제 1 항에 있어서, L1,L2및 L3가 모두 치환된 또는 비치환된 비피리딜이고 n이 4 내지 7인 화합물.
  3. 하기식의 화합물 ;
    이때 M은 Ru,Os 및 Re로 구성된 군으로부터 선택되고 ; L1,L2및 L3는 동일하거나 상이하고 각각은
    이때 R1,R2,R3,R4,R5및 R6는 동일하거나 상이하고 각각은 H 또는 1-5 탄소원자의 알칼기이나, 단 R1-R6중 하나는 하기식의 링커이고 :
    (이때 n은 1-20의 정수이고 X는 O,S,SO2,COO 및 CONH로 구성된 군으로부터 선택되고 ; y는 1-20의 정수이고 ; Z는 0 또는 1이고 ; B는 이분자 또는 그의 합성 동족체이고, 포스포디에스테르기는 동일한 이분자에 결합되며 ; 상기 화합물은 전기화학발광할 수 있다.
  4. 하기식의 착물
    이때 M은 루테늄, 오스뮴 또는 레늄이고 ; R1,R2,R3,R4및 R5는 동일하거나 상이하고 각각은 H 또는 1-4 탄소원자의 알킬이고 ; n은 1 내지 20의 정수이고 ; X는 O,S,SO2,COO 및 CONH로 구성된 군으로부터 선택되고 ; y는 1-20의 정수이고, z는 0 또는 1이고 n은 1-1000의 정수이고 ; NAB는 변형된 또는 변형되지 않은 핵산 염기이며 ; 상기 착물은 전기화학발광할 수 있다.
  5. 제 4 항에 있어서, 상기 핵산이 중합효소 증폭반응을 위한 프라이머인 착물.
  6. 제 4 항에 있어서, 상기 핵산이 혼성체화 프로브인 방법.
  7. 프로브가 관심있는 상기 분석물에 선택적으로 결합하여 관심있는 분석물-프로브 복합체를 형성하는 조건하에서 샘플을 하기식의 프로브와 접촉시키고 :
    (이때 M은 루테늄, 오스뮴 또는 레늄이고 ; R1,R2,R3,R4및 R5는 동일하거나 상이하고 각각은 H 또는 1-4 탄소원자의 알킬이고 ; n은 1 내지 20의 정수이고 ; X는 O,S,SO2,COO 및 CCNH로 구성된 군으로부터 선택되고 ; y는 1-20의 정수이고, z는 0 또는 1이고, m은 1-1000의 정수이고 ; NAB는 변형된 또는 변형되지 않은 핵산 염기임) ; 상기 전기화학발광을 측정하는 단계들로 구성되는, 샘플내에 존재하는 관심있는 핵산 분석물을 검출하는 방법.
  8. 제 7 항에 있어, 상기 관심있는 분석물이 핵산 서열이고 상기 프로브가 상기 관심있는 분석물에 상보적인 핵산 프로브인 방법.
  9. 하기 (a)-(c)로 구성되는, 중합효소 연쇄 반응 또는 기타 프라이머-의존성 반응의 증폭 생성물내의 관심있는 핵산을 검출하기 위해 중합효소 연쇄 반응 또는 기타 프라이머-의존성 반응을 수행하는 방법 : (a) 상기 중합효소 연쇄 반응 또는 기타 프라이머-의존성 반응에 하기식의 프라이머를 혼입시키고 :
    (이때 M은 루테늄, 오스뮴 또는 레늄이고 ;R1,R2,R3,R4및 R5는 동일하거나 상이하고 각각은 H 또는 1-4 탄소원자의 알킬이고 ; n은 1 내지 20의 정수이고, X는 O,S,SO2,COO 및 CCNH로 구성된 군으로부터 선택되고 y는 1-20의 정수이고, z는 0 또는 1이고, m은 1-1000의 정수이고 ; NAB는 변형된 또는 변형되지 않은 핵산 염기임) ; (b) 중합효소 연쇄 반응 또는 기타 프라이머-의존성 반응을 수행하고 ; (c) 관심있는 증폭된 핵산의 전기화학발광을 측정한다.
  10. 하기 단계들로 구성되는, 중합효소 연쇄 반응 또는 기타 프라이머-의존성 반응의 생성물내의 관심있는 핵산 검출 방법 : (a) 상기 반응에서의 적어도 하나의 핵산을 하기식의 화합물로써 라벨링하고 ;
    (이때 M은 루테늄, 오스뮴 또는 레늄이고 ; R1,R2,R3,R4및 R5는 동일하거나 상이하고 각각은 H 또는 1-4 탄소원자의 알킬이고 ; n은 1 내지 20의 정수이고, X는 O,S,SO2,COO 및 CCNH로 구성된 군으로부터 선택되고, y는 1-20의 정수이고, z는 0 또는 1이고, m은 1-1000의 정수이고 ; NAB는 변형된 또는 변형되지 않은 핵산 염기임) ; (b) 상기 핵산을 증폭 생성물내로 혼입시키는 중합효소 연쇄 반응 또는 기타 프라이머-의존성 반응을 수행하고 ; (c) 관심있는 상기 핵산의 전기화학발광을 측정한다.
  11. 제 1 항에 있어서, 화합물의 상대 ECL 능력대 그의 비피리딜 동족체의 상대 ECL 능력의 비율이 0.10 이상인 화합물.
  12. 제 3 항에 있어서, 화합물의 상대 ECL 능력대 그의 비피리딜 동족체의 상대 ECL 능력의 비율이 0.10 이상인 화합물.
  13. 제 7 항에 있어서, 관심있는 분석물-프로브 착물의 상대 ECL 능력대 그의 비피리딜 동족체의 상대 ECL 능력의 비율이 0.10 이상인 방법.
  14. 제 4 항에 있어서, 착물의 상대 ECL 능력대 그의 비피리딜 동족체의 상대 ECL 능력의 비율이 0.25 이상인 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019940702034A 1991-12-11 1992-12-07 Dna 프로브 검정을 위한 개선된 전기화학발광 라벨 Expired - Fee Related KR0169755B1 (ko)

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Families Citing this family (113)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6451225B1 (en) * 1986-04-30 2002-09-17 Igen International, Inc. Electrochemiluminescent reaction utilizing amine-derived reductant
US6890712B1 (en) * 1993-09-22 2005-05-10 Bioveris Corporation Cycling DNA/RNA amplification electrochemiluminescent probe assay
US6071699A (en) 1996-06-07 2000-06-06 California Institute Of Technology Nucleic acid mediated electron transfer
US6319670B1 (en) 1995-05-09 2001-11-20 Meso Scale Technology Llp Methods and apparatus for improved luminescence assays using microparticles
AU726047B2 (en) * 1995-11-15 2000-10-26 Gen-Probe Incorporated Nucleic acid probes complementary to human papillomavirus nucleic acid and related methods and kits
US6994958B2 (en) * 1995-11-29 2006-02-07 Anthony Nolan Bone Marrow Trust Methods for separating and/or identifying DNA molecules
DE19627290A1 (de) * 1996-07-06 1998-01-08 Boehringer Mannheim Gmbh Bestimmung von Analyten unter Verwendung zweier Markierungen
US7314711B2 (en) * 1997-05-23 2008-01-01 Bioveris Corporation Assays employing electrochemiluminescent labels and electrochemiluminescence quenchers
JP3785517B2 (ja) * 1997-06-05 2006-06-14 東ソー株式会社 核酸融解温度測定法
EP1029244A4 (en) * 1997-10-02 2003-07-23 Aclara Biosciences Inc CAPILLAR ASSAY WITH SEPARATION OF FREE AND TIED SPECIES
NZ504214A (en) * 1997-10-24 2003-06-30 Invitrogen Corp Recombination cloning using nucleic acids having recombination sites
WO1999037820A1 (en) * 1998-01-26 1999-07-29 Saigene Corporation An exponential nucleic acid amplification method using a single primer of opposing polarity
DE19811582A1 (de) * 1998-03-17 1999-09-23 Roche Diagnostics Gmbh Stabilisierung und Verstärkung von Elektrochemilumineszenz-Signalen
US6391544B1 (en) * 1998-05-15 2002-05-21 Abbott Laboratories Method for using unequal primer concentrations for generating nucleic acid amplification products
US8445664B2 (en) * 1998-06-24 2013-05-21 Enzo Diagnostics, Inc. Kits for amplifying and detecting nucleic acid sequences
US6743605B1 (en) * 1998-06-24 2004-06-01 Enzo Life Sciences, Inc. Linear amplification of specific nucleic acid sequences
US6288221B1 (en) 1998-08-20 2001-09-11 Duke University Methods of synthesis of halogen base-modified oligonucleotides and subsequent labeling with a metal-catalyzed reaction
US6312896B1 (en) * 1998-09-17 2001-11-06 Igen Inaternational, Inc. Assays for measuring nucleic acid binding proteins and enzyme activities
US6214552B1 (en) 1998-09-17 2001-04-10 Igen International, Inc. Assays for measuring nucleic acid damaging activities
US6232075B1 (en) * 1998-12-14 2001-05-15 Li-Cor, Inc. Heterogeneous assay for pyrophosphate detection
EP1035214B1 (en) * 1999-03-05 2005-12-21 Tosoh Corporation A 3'-end modified nucleic acid probe, suitable for hybridization and detection via fluorescence
US6410255B1 (en) * 1999-05-05 2002-06-25 Aurora Biosciences Corporation Optical probes and assays
US7141436B2 (en) * 1999-11-03 2006-11-28 Science And Technology Corp. Immunoassay and reagents and kits for performing the same
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US20030082549A1 (en) * 2000-08-30 2003-05-01 Xiangjun Liu Method for determining alleles
US7029919B2 (en) * 2001-05-04 2006-04-18 Agilent Technologies, Inc. Electro-optical device and methods for hybridization and detection
JP4628594B2 (ja) * 2001-06-25 2011-02-09 昭和電工株式会社 有機発光素子および発光材料
US7223483B2 (en) 2001-06-25 2007-05-29 Showa Denko K.K. Light-emitting material and organic light-emitting device
US7041280B2 (en) * 2001-06-29 2006-05-09 Genzyme Corporation Aryl boronate functionalized polymers for treating obesity
US6808939B2 (en) * 2001-06-29 2004-10-26 Igen International, Inc. ECL labels having improved non-specific binding properties, methods of using and kits containing the same
DK2461156T3 (da) 2001-06-29 2020-08-03 Meso Scale Technologies Llc Indretning til luminescenstestmålinger
EP3021106B8 (en) * 2001-07-30 2019-03-27 Meso Scale Technologies, LLC Methods of using assay electrode having immobilized lipid/protein layers for luminescence test measurements
ES2268076T3 (es) * 2001-08-10 2007-03-16 F. Hoffmann-La Roche Ag Metodo para la fabricacion de microparticulas cargadas de proteinas.
EP1436620B1 (en) 2001-09-10 2014-04-23 Meso Scale Technologies, LLC Methods and apparatus for conducting multiple measurements on a sample
US20030086165A1 (en) * 2001-11-05 2003-05-08 Cross John W. Illuminated reticle
US10022078B2 (en) 2004-07-13 2018-07-17 Dexcom, Inc. Analyte sensor
US20040241723A1 (en) * 2002-03-18 2004-12-02 Marquess Foley Leigh Shaw Systems and methods for improving protein and milk production of dairy herds
JP2006506644A (ja) * 2002-11-15 2006-02-23 アプレラ コーポレイション 核酸配列検出
US20040161737A1 (en) * 2003-02-14 2004-08-19 Yang Dan-Hui D. Novel luminescent metal chelates and methods for their detection
EP1606419A1 (en) 2003-03-18 2005-12-21 Quantum Genetics Ireland Limited Systems and methods for improving protein and milk production of dairy herds
US7619059B2 (en) * 2003-07-29 2009-11-17 Life Technologies Corporation Bimolecular optical probes
US7727752B2 (en) * 2003-07-29 2010-06-01 Life Technologies Corporation Kinase and phosphatase assays
CA2445420A1 (en) 2003-07-29 2005-01-29 Invitrogen Corporation Kinase and phosphatase assays
EP2484687A3 (en) 2003-08-08 2012-11-14 Life Technologies Corporation Methods and compositions for seamless cloning of nucleic acid molecules
US7920906B2 (en) 2005-03-10 2011-04-05 Dexcom, Inc. System and methods for processing analyte sensor data for sensor calibration
EP2224243A1 (en) * 2003-09-12 2010-09-01 Life Technologies Corporation Identifying modulators of enzymatic activity
US9247900B2 (en) 2004-07-13 2016-02-02 Dexcom, Inc. Analyte sensor
US8304189B2 (en) * 2003-12-01 2012-11-06 Life Technologies Corporation Nucleic acid molecules containing recombination sites and methods of using the same
MXPA06009452A (es) 2004-02-19 2007-03-15 Univ Alberta Polimorfismos del promotor de leptin y sus usos.
US9414777B2 (en) 2004-07-13 2016-08-16 Dexcom, Inc. Transcutaneous analyte sensor
US20080227219A1 (en) * 2004-11-17 2008-09-18 Frank Gamez Electrochemiluminescent assay
US7354719B2 (en) 2004-12-08 2008-04-08 Gen-Probe Incorporated Detection of nucleic acids from multiple types of human papillomaviruses
US20060275841A1 (en) * 2004-12-20 2006-12-07 Martin Blankfard Assay method and apparatus with reduced sample matrix effects
EP1854160B1 (en) * 2005-02-10 2017-06-21 Yeda Research And Development Co., Ltd. Redox-active structures and devices utilizing the same
BRPI0709282B8 (pt) 2006-03-29 2021-05-25 Merial Ltd vacina contra estreptococos
GB0701444D0 (en) * 2007-01-25 2007-03-07 Iti Scotland Ltd Detecting analytes
AU2009234422B2 (en) 2008-04-11 2014-05-08 Board Of Regents Of The University Of Texas System Method and apparatus for nanoparticle electrogenerated chemiluminescence amplification
EP2626693A3 (en) 2008-05-08 2014-10-01 Board of Regents of the University of Texas System Luminescent nanostructured materials for use in electrogenerated chemiluminescence
US20100261292A1 (en) 2009-04-10 2010-10-14 Meso Scale Technologies, Llc Methods for Conducting Assays
CA2769380C (en) 2009-07-27 2020-08-25 Meso Scale Technologies, Llc Assay apparatuses, consumables and methods
JP2011072222A (ja) * 2009-09-29 2011-04-14 Kitasato Otsuka Biomedical Assay Kenkyusho:Kk 標的核酸の検出方法
WO2012005588A2 (en) 2010-07-07 2012-01-12 Vereniging Voor Christelijk Hoger Onderwijs, Wetenschappelijk Onderzoek En Patiëntenzorg Novel biomarkers for detecting neuronal loss
JP5943927B2 (ja) 2010-10-14 2016-07-05 メソ スケール テクノロジーズ エルエルシー アッセイデバイスにおける試薬貯蔵
HUE029030T2 (en) 2010-10-25 2017-01-30 Hoffmann La Roche Use of signal repair compounds for electrochemiluminescence detection
ES2531960T3 (es) * 2011-02-11 2015-03-23 Fábrica Nacional De Moneda Y Timbre - Real Casa De La Moneda Pigmentos luminiscentes y su uso en aplicaciones de seguridad
DK3467500T3 (da) 2011-11-11 2021-08-16 Eli N Glezer Cobinder-understøttede assayfremgangsmåder
US9625465B2 (en) 2012-05-15 2017-04-18 Defined Diagnostics, Llc Clinical diagnostic systems
US9213043B2 (en) 2012-05-15 2015-12-15 Wellstat Diagnostics, Llc Clinical diagnostic system including instrument and cartridge
US9081001B2 (en) 2012-05-15 2015-07-14 Wellstat Diagnostics, Llc Diagnostic systems and instruments
WO2014107576A2 (en) 2013-01-04 2014-07-10 Meso Scale Technologies, Llc Assay apparatuses, methods and reagents
KR102275006B1 (ko) 2013-03-11 2021-07-09 메소 스케일 테크놀러지즈, 엘엘시 다중화 어세이를 수행하기 위한 개선된 방법
US9618510B2 (en) 2013-03-13 2017-04-11 Meso Scale Technologies, Llc. Assay methods
US10114015B2 (en) 2013-03-13 2018-10-30 Meso Scale Technologies, Llc. Assay methods
CN105209532B (zh) 2013-03-15 2017-11-28 路博润先进材料公司 不含重金属的cpvc组合物
EP3066219B1 (en) 2013-11-08 2018-12-26 Ionis Pharmaceuticals, Inc. Methods for detecting oligonucleotides
IL229525A0 (en) 2013-11-20 2014-01-30 Yeda Res & Dev Metal complexes of tris-bipyridyl and their uses in electrochromic applications
US10955412B2 (en) 2014-05-09 2021-03-23 Meso Scale Technologies, Llc. Graphene-modified electrodes
AU2015259048B2 (en) 2014-05-15 2021-09-09 Meso Scale Technologies, Llc. Improved assay methods
PL3338094T3 (pl) 2015-08-20 2019-09-30 F. Hoffmann-La Roche Ag Test immunologiczny oparty na cząstkach z zastosowaniem pegylowanego swoistego względem analitu czynnika wiążącego
KR102437995B1 (ko) 2016-03-07 2022-08-29 에프. 호프만-라 로슈 아게 항-p53 항체의 검출
WO2017153574A1 (en) 2016-03-11 2017-09-14 Roche Diagnostics Gmbh Branched-chain amines in electrochemiluminescence detection
CN110234995A (zh) 2017-02-02 2019-09-13 豪夫迈·罗氏有限公司 使用至少两种聚乙二醇化的分析物特异性结合试剂的免疫测定
FI3756009T3 (fi) 2018-02-23 2023-12-01 Meso Scale Technologies Llc Menetelmiä antigeeniä sitovien molekyylien seulontaan normalisoimalla antigeeniä sitovan molekyylin pitoisuus
CN111936522B (zh) 2018-04-18 2024-07-02 豪夫迈·罗氏有限公司 新型抗胸苷激酶抗体
CN112639475A (zh) 2018-08-31 2021-04-09 豪夫迈·罗氏有限公司 Dlbcl的预后指数中的胸苷激酶(tk-1)
CN109232663B (zh) * 2018-11-08 2020-09-25 云南大学 一种钌配合物的制备方法及其艾滋病毒逆转录酶抑制应用
AU2019418498A1 (en) 2019-01-03 2021-07-15 Meso Scale Technologies, Llc Compositions and methods for carrying out assay measurements
EP3931201A1 (en) 2019-03-01 2022-01-05 Meso Scale Technologies, LLC Electrochemiluminescent labeled probes for use in immunoassay methods, methods using such and kits comprising same
CN113939599B (zh) 2019-06-07 2025-06-06 豪夫迈·罗氏有限公司 杂交全lna寡核苷酸
US11896979B2 (en) 2019-07-16 2024-02-13 Meso Scale Technologies, Llc. Assay apparatuses, methods, and reagents
EP4004554A1 (en) 2019-07-22 2022-06-01 F. Hoffmann-La Roche AG S100a9 as blood biomarker for the non-invasive diagnosis of endometriosis
WO2021013784A1 (en) 2019-07-22 2021-01-28 F. Hoffmann-La Roche Ag S100a8 as blood biomarker for the non-invasive diagnosis of endometriosis
WO2021013786A1 (en) 2019-07-22 2021-01-28 F. Hoffmann-La Roche Ag S100a6 as blood biomarker for the non-invasive diagnosis of endometriosis
KR20220024699A (ko) 2019-07-22 2022-03-03 에프. 호프만-라 로슈 아게 자궁내막증의 비침습적 진단을 위한 혈액 바이오마커로서의 s100a12
WO2021013781A1 (en) 2019-07-22 2021-01-28 F. Hoffmann-La Roche Ag Substance p as blood biomarker for the non-invasive diagnosis of endometriosis
KR20220100883A (ko) 2019-11-15 2022-07-18 에프. 호프만-라 로슈 아게 환자 샘플의 질량분석법 측정을 위한 베타-락탐 항생제의 유도체화
US20240018571A1 (en) 2020-05-19 2024-01-18 Meso Scale Technologies, Llc. Methods, compositions, and kits for nucleic acid detection
CN116113830A (zh) 2020-07-01 2023-05-12 中尺度技术有限责任公司 用于测定测量的组合物和方法
JP2023547505A (ja) 2020-11-02 2023-11-10 エフ． ホフマン－ラ ロシュ アーゲー ＳＡＲＳ－ＣｏＶ－２ヌクレオカプシド抗体
JP2023554517A (ja) 2020-12-22 2023-12-27 エフ． ホフマン－ラ ロシュ アーゲー 試料中の目的の分析物を検出するための方法
WO2022207628A1 (en) 2021-03-30 2022-10-06 F. Hoffmann-La Roche Ag Scf as blood biomarker for the non-invasive diagnosis of endometriosis
WO2022207685A1 (en) 2021-04-01 2022-10-06 F. Hoffmann-La Roche Ag Psp94 as blood biomarker for the non-invasive diagnosis of endometriosis
EP4341700A1 (en) 2021-05-17 2024-03-27 F. Hoffmann-La Roche AG Sfrp4 as blood biomarker for the non-invasive diagnosis of adenomyosis
US20250042976A1 (en) 2021-10-26 2025-02-06 Roche Diagnostics Operations, Inc. Monoclonal antibodies specific for sars-cov-2 rbd
KR20240146115A (ko) 2021-12-30 2024-10-07 메소 스케일 테크놀러지즈, 엘엘시 전기화학발광 검출 방법
WO2023131594A1 (en) 2022-01-05 2023-07-13 F. Hoffmann-La Roche Ag Derivatization of compounds in patient samples for therapeutic drug monitoring (tdm)
CN118696234A (zh) 2022-02-18 2024-09-24 豪夫迈·罗氏有限公司 用于检测样品中的目标分析物的方法
CN119404103A (zh) 2022-06-23 2025-02-07 豪夫迈·罗氏有限公司 用于诊断子宫内膜异位症及用于对子宫内膜异位症的分期进行分类的方法
JP2025524025A (ja) 2022-07-22 2025-07-25 エフ． ホフマン－ラ ロシュ アーゲー 多嚢胞性卵巣症候群の診断のための（血液）バイオマーカーとしてのロイコトリエンａ４ヒドロラーゼ（ｌｔａ４ｈ）
CN119585618A (zh) 2022-07-22 2025-03-07 豪夫迈·罗氏有限公司 成纤维细胞生长因子结合蛋白1(fgfbp1)作为（血液）生物标志物用于诊断多囊卵巢综合征
WO2024017983A1 (en) 2022-07-22 2024-01-25 F. Hoffmann-La Roche Ag Meteorin-like protein (metrnl) as (blood) biomarker for the diagnosis of polycystic ovarian syndrome
WO2025017135A1 (en) 2023-07-20 2025-01-23 F. Hoffmann-La Roche Ag Screening methods for antibody compositions
WO2025026908A1 (en) 2023-07-28 2025-02-06 Roche Diagnostics Gmbh Serum epha1 as biomarker for endometriosis

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3329892A1 (de) * 1983-08-18 1985-03-07 Köster, Hubert, Prof. Dr., 2000 Hamburg Verfahren zur herstellung von oligonucleotiden
FR2557836B1 (fr) * 1983-11-17 1988-08-05 Legrand Pierre Equipement de vehicules de transport en commun et de lieux de transit, et application particuliere
DK365785A (da) * 1984-09-17 1986-03-18 Hoffmann La Roche Metalcomplexer
US5238808A (en) * 1984-10-31 1993-08-24 Igen, Inc. Luminescent metal chelate labels and means for detection
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
JPS6228549A (ja) * 1985-07-30 1987-02-06 Teijin Seiki Co Ltd 駆動源付き減速装置
KR960016340B1 (ko) * 1986-04-30 1996-12-09 이겐 인코오포레이팃드 전기화학발광 분석법
DK564086A (da) * 1986-11-25 1988-06-17 Novo Industri As Enzymatisk detergent-additiv
US5135717A (en) * 1986-12-24 1992-08-04 British Technology Group Usa Inc. Tetrabenztriazaporphyrin reagents and kits containing the same
DE3856567T2 (de) * 1987-11-04 2004-12-16 Igen International, Inc. Lumineszente Rhenium-Komplexe und Verfahren zur Herstellung
US4951878A (en) * 1987-11-16 1990-08-28 Casey Gary L Pico fuel injector valve
US4835214A (en) * 1987-11-23 1989-05-30 Eastman Kodak Company Polymer blends useful for hot fillable containers having improved gas barrier properties
AU622899B2 (en) * 1988-05-04 1992-04-30 F. Hoffmann-La Roche Ag Rutheniumcomplex-compounds
US5247243A (en) * 1988-11-03 1993-09-21 Igen, Inc. Method and apparatus for conducting electrochemiluminescent measurements
KR0143993B1 (ko) * 1988-11-03 1998-07-15 리차아드 제이 매씨이 전기화학 발광성 분석
US5061445A (en) * 1988-11-03 1991-10-29 Igen, Inc. Apparatus for conducting measurements of electrochemiluminescent phenomena
CA2033692A1 (en) * 1990-01-25 1991-07-26 Wilhelm Bannwarth Energy transfer systems
IL100867A (en) * 1991-02-06 1995-12-08 Igen Inc Method and apparatus for improved luminescence assays

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DE69232086D1 (de) 2001-10-31
IL103960A0 (en) 1993-05-13
ES2164069T3 (es) 2002-02-16
AU3238893A (en) 1993-07-19
EP0667919B1 (en) 2001-09-26
JPH07503947A (ja) 1995-04-27
KR0169755B1 (ko) 1999-01-15
US5597910A (en) 1997-01-28
EP0667919A1 (en) 1995-08-23
WO1993012256A1 (en) 1993-06-24
CA2123808A1 (en) 1993-06-24
AU661757B2 (en) 1995-08-03
DE69232086T2 (de) 2003-09-11
ZA929351B (en) 1993-06-04
JP3067030B2 (ja) 2000-07-17
CA2123808C (en) 2003-05-27
US5686244A (en) 1997-11-11
US5610017A (en) 1997-03-11
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ATE206170T1 (de) 2001-10-15
IL103960A (en) 2000-08-31

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