KR20160121636A - Cosmetic composition for skin moisturizing comprising the extract of Taraxacum platycarpum H. Dahlstedt, Heartleaf Houttuynia, Glycyrrhiza uralensis Fischer, root bark of Ulmus davidiana - Google Patents

Abstract

The present invention relates to a method for the treatment of Taraxacum platycarpum H. Dahlstedt, Heartleaf Houttuynia , Glycyrrhiza The present invention relates to a cosmetic composition for improving skin moisturization, which comprises a mixed extract of uralensis Fischer and root bark of Ulmus davidiana as an active ingredient. The cosmetic composition for improving skin moisturization is characterized in that a mixed extract of Pseudomonas aeruginosa, It was confirmed that the expression of involucrin and loricrin, which are proteins constituting the cell membrane, is increased, cell proliferation is promoted, and that water treatment of the skin is inhibited when treated with mouse, , Ginseng root, licorice root, and yugaekpyu were found to be useful as a cosmetic composition for skin moisturization.

Description

[0001] The present invention relates to a cosmetic composition for improving the skin moisturizing effect, which comprises a mixed extract of Pseudomonas aeruginosa, Pseudomonas aeruginosa, Lycopersicon esculentum, Licorice root, }

The present invention relates to the use of Taraxacum platycarpum H. dahlstedt), Heartleaf Houttuynia , licorice ( Glycyrrhiza The present invention relates to a cosmetic composition for skin moisturization improvement which contains, as an active ingredient, a mixed extract of uralensis Fischer and root bark of Ulmus davidiana .

Skin is the body tissue that is present on the outermost layer of the human body and acts as a barrier between the external environment and internal body tissues and protects tissues from external chemicals, pathogens, and UVs. The epidermis is a multilayer epithelial cell composed of various cells such as keratinocytes and melanocytes, and plays a key role in this protection. Keratin cells produce skin protective substances through differentiation, and are closely related to the process of inhibiting the cell cycle and the onset of expression of many genes. For example, when the expression of keratin 5 and keratin 14 is decreased, keratin 1, keratin 10, involucrin, loricrin, Expression of differentiation-related genes such as filaggrin increases with time. When an obstacle to the regulation of keratinocyte differentiation occurs, it causes the skin barrier function and the deterioration of the immune response, leading to skin diseases such as psoriasis and atopic dermatitis. Immunologic agents such as cyclosporine A, tacrolimus and pimecrolimus have now been marketed worldwide as agents for skin differentiation-related diseases, and skin moisturizers have a positive effect on related diseases and skin texture It is known. In the keratinocyte differentiation, the cell envelope pila green plays a role in maintaining skin health by generating a natural moisturizing factor. Therefore, the development of keratinocyte differentiation studies will increase the possibility of development of therapeutic agents and therapies for skin differentiation-related diseases.

To solve such skin diseases, many medicinal plants have recently been developed as pharmaceutical and cosmetic raw materials. There are many kinds of medicinal plants, but the medicinal plants which are being used and being studied as ingredients for cosmetics are still known as a part. Research on medicinal plants has a very high potential for the treatment of skin diseases and the development of raw materials for cosmetics, and it is necessary to carry out a systematic research using them.

In Korea, Poe is a dandelion of Taraxacum platycarpum H. Dahlstedt) or the outpost of an inbred plant, with little odor and taste. It is used for symptoms such as swelling, mastitis, sore throat, sore throat (appendicitis, lung abscess, peritonitis), eye congestion, acute hepatitis, jaundice, fever. In addition, pharmacological actions have been reported as insecticidal action, immune function enhancement, bile secretion action, liver function protection action, and diuretic action.

Heartleaf Houttuynia ) is a Saururus chinensis, a part of the perennial plant that grows in the shady and watery areas of the mountains of South Korea including Jeju and Ulleungdo in Korea. Its pharmacological action is antimicrobial action, inhibiting the development of Staphylococcus aureus, hemolytic streptococci, etc., and is effective for enhancing immune function, anti-inflammatory action, diuretic action, swallowing action and chronic bronchitis. As a cosmetic, it is known that suppression of sebum secretion and antibacterial, deodorant and inflammation inhibitory effects are known.

Glycyrrhiza is a soybean and perennial herbaceous plant that has excellent anti-inflammatory, anti-allergic and anti-bacterial effects and is highly effective in promoting collagen synthesis and synthesis of hyaluronic acid, which are closely related to skin elasticity, wrinkle and skin moisturizing effects. Glycyrrhizin, Flavonoid, and mannitol are the physiologically active ingredients of licorice.

Ulmus davidiana refers to the root bark of elm, and the shell of elm trunk is called as milky white bark. It grows frequently in mountain valleys and waterfronts in the central and northern parts of Korea. In Dong-bo-bo, the elm trees are well-balanced and tasteful and have no poison, so they can pass the feces easily, and they are effective in enteritis by eliminating the intestines and upper quadrats. They are effective for various swelling and nausea and are not addictive. have.

Accordingly, the present inventors have made efforts to develop a composition for skin moisturization improvement that is economical and has less side effects by using medicinal plants, and found that the mixed extracts of Pogongyoung, Hwasungsu, licorice, and yugaepi do not show cytotoxicity, The present inventors completed the present invention by confirming that it increases the expression of involucrin and loricrin, which are proteins which are proteins, and promotes cell proliferation and suppresses moisture loss of the skin when treated with a mouse.

It is an object of the present invention to provide a process for the preparation of a plant of the genus Taraxacum platycarpum H. Dahlstedt, Heartleaf Houttuynia , Glycyrrhiza uralensis Fischer and root bark of Ulmus The present invention also provides a cosmetic composition for skin moisturization improvement which comprises a mixture of at least one compound selected from the group consisting of < RTI ID = 0.0 >

In order to accomplish the above object, the present invention provides a method for producing a compound of the formula (I), wherein the compound of the present invention is Taraxacum platycarpum H. Dahlstedt, Heartleaf Houttuynia , Glycyrrhiza uralensis Fischer and root bark of Ulmus davidiana ) as an active ingredient.

Further, the present invention provides a pharmaceutical composition for preventing and treating skin diseases, which comprises a mixed extract of Pseudomonas sp.

In addition, the present invention provides a skin external preparation for skin moisturizing, which contains, as an active ingredient, a mixed extract of Pseudomonas sp.

The present invention relates to a method for the treatment of Taraxacum platycarpum H. Dahlstedt, Heartleaf Houttuynia , Glycyrrhiza The present invention relates to a cosmetic composition for improving skin moisturization, which comprises a mixed extract of uralensis Fischer and root bark of Ulmus davidiana as an active ingredient. The cosmetic composition for improving skin moisturization is characterized in that a mixed extract of Pseudomonas aeruginosa, It was confirmed that the expression of involucrin and loricrin, which are proteins constituting the cell membrane, is increased, cell proliferation is promoted, and that water treatment of the skin is inhibited when treated with mouse, , Hwasungcho, licorice, and yugaepi can be effectively used as a cosmetic composition for skin moisturization.

1 is a diagram showing cytotoxicity of a mixed herbal medicine extract:
CTL: control group; And
ECMP: Mixed herbal medicine extract.
FIG. 2 is a graph showing the expression of a skin cell membrane constituting protein of SV-HEKs cells treated with a mixed herbal medicine extract.
CTL: control group;
Ca ^{2 +:} calcium; And
ECMP: Mixed herbal medicine extract.
FIG. 3 is a graph showing Involucrin promoter activity of SV-HEKs cells treated with the mixed crude drug extract.
CTL: control group;
Ca ^{2 +:} calcium; And
ECMP: Mixed herbal medicine extract.
FIG. 4 is a graph showing the loricrin promoter activity of SV-HEKs cells treated with the mixed crude drug extract.
CTL: control group;
Ca ^{2 +:} calcium; And
ECMP: Mixed herbal medicine extract.
FIG. 5 shows cell proliferation of SV-HEKs cells treated with mixed crude drug extract. FIG.
CTL: control group;
Ca ^{2 +:} calcium; And
ECMP: Mixed herbal medicine extract.
FIG. 6 is a diagram showing measurement of skin function recovery after applying a mixed herbal medicine extract to the skin tissue of a mouse. FIG.
Vehicle: Control group; And
ECMP: Mixed herbal medicine extract.
FIG. 7 is a graph showing the expression of keratin-binding protein by immuno-staining after treating the skin tissue of a mouse with a mixed herbal medicine extract.
Vehicle: Control group; And
ECMP: Mixed herbal medicine extract.

Hereinafter, the present invention will be described in detail.

The present invention relates to a method for the treatment of Taraxacum platycarpum H. Dahlstedt, Heartleaf Houttuynia , Glycyrrhiza The present invention provides a cosmetic composition for skin moisturization, which contains, as an active ingredient, a mixed extract of uralensis Fischer and root bark of Ulmus davidiana .

In the above-mentioned extraction, water, alcohol or a mixture thereof is preferably used as an extraction solvent for the mixed extract of Porphyrae, Lycopersicon esculentum, Lycopersicon esculentum, and Rootgrass. The alcohol is preferably a C ₁ to C ₂ lower alcohol, and it is preferable to use a lower alcohol or ethanol or methanol. As the extraction method, it is preferable to use shaking extraction, Soxhlet extraction or reflux extraction, but it is not limited thereto. It is preferable that the extraction solvent is added by 1 to 10 times, preferably 4 to 6 times, more preferably by adding to the mixed amount of the above-mentioned extraction solvent, The extraction temperature is preferably 20 to 100 ° C, more preferably 20 to 40 ° C, most preferably room temperature, but is not limited thereto. The extraction time is preferably 10 to 48 hours, more preferably 15 to 30 hours, most preferably 24 hours, but is not limited thereto. The number of times of extraction is preferably 1 to 5 times, more preferably 3 to 4 times, more preferably 3 times, but is not limited thereto. In addition, the above-mentioned mixed extract is characterized in that it is extracted with a mixture of Pseudomonas aeruginosa, Pseudomonas sp., Lycopersicon esculentum, Lycopersicon esculentum, and Rootgrass. However, the respective extracts of Pseudomonas sp.

Further, in the above method, it is preferable to use a vacuum decompression concentrator or a vacuum rotary evaporator, but not limited thereto. The drying is preferably performed under reduced pressure, vacuum drying, boiling, spray drying or freeze drying, but not always limited thereto.

In addition, the mixture of Pseudomonas aeruginosa, Pseudomonas aeruginosa, Lycopersicon esculentum and Lycopersiconia fructus comprises 25 to 40 parts by weight of Pseudomonas aeruginosa, 25 to 40 parts by weight of Psoriasis, 10 to 20 parts by weight of licorice and 10 to 20 parts by weight of Rootini, More preferably 30 to 38 parts by weight of porky-yong, 30 to 38 parts by weight of psyllium seeds, 13 to 18 parts by weight of licorice and 13 to 18 parts by weight of rhizome, 32 to 35 parts by weight of glutinous rice, 15 to 17 parts by weight of licorice and 15 to 17 parts by weight of curl.

In addition, the composition is characterized by promoting keratinocyte differentiation.

In a specific embodiment of the present invention, the present inventors obtained an extract by grinding a mixture of Pseudomonas aeruginosa, Pseudomonas sp., Lycopersicon esculentum, Lycopersicon esculentum and Radix Pseudomonas aeruginosa by hot water extraction using a mixed solvent of water and alcohol and then freeze- It was confirmed that cytotoxicity was not observed by treatment with HEKx cells (see Fig. 1). In addition, it was confirmed that SV-HEKx cells were treated with the above mixed extract to increase protein expression of inbolucurin and lorikreen, and promoted cell differentiation by increasing promoter activity (see FIGS. 2, 3 and 4) It was confirmed that the growth of cells treated with the mixed extract was similar to that of calcium-treated positive control (see FIG. 5). In addition, it was confirmed that recovery of moisture loss was accelerated in the skin tissue of the mice treated with the mixed extract (see FIG. 6), and the expression of keratin-binding protein was increased (see FIG. 7). Accordingly, the mixed extract of Phellinus lachrymum, Pseudomonas sp., Lycopersicon esculentum, Lycopersicon esculentum and Lycopersicon esculentum of the present invention can be effectively used as a cosmetic composition for skin moisturization.

The cosmetic composition of the present invention includes, but is not limited to, a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids and seaweed extracts. Specific examples of the water-soluble vitamin include vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, nicotinic amide, folic acid, vitamin C and vitamin H And include salts thereof (thiamine hydrochloride, sodium ascorbate, etc.) or derivatives thereof (ascorbic acid-2-phosphate sodium salt, ascorbic acid-2-phosphate magnesium salt, and the like). Specific examples of the above-mentioned useful vitamins include vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol, d-alpha tocopherol) Alpha-tocopherol vitamin E, DL-pantothenyl alcohol, D-pantothenyl alcohol, pantothenyl ethyl ether and the like) But is not limited thereto. The polymeric peptide may be any compound as long as it can be compounded in cosmetics, but is not particularly limited to collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin or keratin. The polymeric polysaccharide may be any compound as long as it can be compounded in cosmetics. Specific examples include hydroxyethylcellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (such as sodium salt) and the like. The sphingolipids may be any of those which can be incorporated in cosmetics, but are not specifically limited to ceramides, phytosphingosine and sphingoglycolipids. The seaweed extract may be any of those which can be compounded in cosmetics, but specifically, it may be a brown algae extract, a red algae extract or a green algae extract, specifically, a color guanine purified from their seaweed extract, arginic acid, sodium alginate, It is not limited.

In the cosmetic preparation composition of the present invention, other ingredients generally added to cosmetics may be blended with the above essential ingredients, if necessary. Specific examples of the other components include a water-soluble organic solvent such as a water-soluble organic solvent such as a water-soluble organic solvent, a preservative, a moisturizing agent, an emollient agent, a surfactant, an organic and inorganic pigment, an organic powder, an ultraviolet absorber, an antiseptic, a bactericide, A blood circulation promoter, a cold agent, an antiperspirant agent, or purified water. Specific examples of the above-mentioned fat-soluble ingredients include, but are not limited to, ester-based fats, hydrocarbon-based fats, silicone based fats, fluorinated fats, animal fats or plant fats. Specific examples of the ester-based oil include glyceryl tri-2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isosertyl isostearate , Butyl stearate, ethyl linoleate, isopropyl linoleate, ethyl oleate, isosilyl myristate, isostearic acid isostearyl, isostearyl palmitate, octyldodecyl myristate, isosertyl isostearate, diethyl sebacinate , Diisopropyl adipate, isoalkyl neopentanoate, tri (capryl, capric acid) glyceryl, tri-2-ethylhexanoate trimethylol propane, triisostearic acid trimethylol propane, tetra 2-ethylhexanoic acid penta Lauric acid, hexyl laurate, decyl myristate, myristyl myristate, myristine acid ethyl myristate, stearyl stearate, decyl oleate, ricinoleate Octyldodecyl oleate, octyldodecyl oleate, octyldodecyl oleate, octyldodecyl oleate, octyldodecyl oleate, octyldodecyl oleate, octyldodecyl oleate, octyldodecyl oleate, octyldodecyl oleate, octyldodecyl oleate, isopropyl stearate, Ethylhexanoate, stearyl 2-ethylhexanoate, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicaprate, di (capryl, capric acid) propylene glycol , Propylene glycol dicaprylylate, dicapric acid neopentyl glycol, dioctanoic acid neopentyl glycol, tricarboxylic acid glyceryl, triunsaturated acid glyceryl, triisopalmitic acid glyceryl, triisostearic acid glyceryl, neopentanoic acid octyl Dodecylsuccinic acid, octadecanoic acid, octadecanoic acid, octadecanoic acid, octadecanoic acid, octadecanoic acid, octadecanoic acid, octadecanoic acid, Stearyl, octyldecyl isostearate, polyglycerin oleic acid ester, polyglycerin isostearic acid ester, triisocetyl citrate, triisolealkyl citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, cetyl lactate, octyldecyl lactate , Triethyl citrate, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, diisostearyl malate, 2-ethylhexyl hydroxystearate, di-2-ethylhexyl succinate, diisobutyl adipate, sebacic acid di But are not limited to, isopropyl myristate, isopropyl myristate, dioctyl sebacate, cholesteryl stearate, cholesteryl isostearate, cholesteryl hydroxystearate, cholesteryl oleate, dihydrocholesteryl oleate, Stearyl, 12-stearoylhydroxystearic acid, isostearyl, 12-stearoylhydroxystearic stearyl Or 12-stearoylhydroxystearic acid is ester-based, such as sostearyl, but is not limited thereto.

Examples of the hydrocarbonaceous oil-based oil include, but are not limited to, hydrocarbons such as squalene, liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, floating isoparaffin, polybutene, microcrystalline wax or vaseline . Specific examples of the silicone-based oil include polymethyl silicone, methylphenyl silicone, methyl cyclopolysiloxane, octamethyl polysiloxane, decamethyl polysiloxane, dodecamethyl cyclosiloxane, dimethylsiloxane-methyl cetyloxysiloxane copolymer, dimethylsiloxane-methyl stearoxysiloxane Copolymers, alkyl-modified silicone oils, or amino-modified silicone oils. Specific examples of the fluorine-based oil include perfluoropolyether, but are not limited thereto. Specific examples of the animal or plant oil include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, new flower oil, soybean oil, corn oil, rape oil, apricot kernel oil, palm kernel oil, palm oil, castor oil, , Grape seed oil, cottonseed oil, palm oil, cucumber nut oil, wheat germ oil, rice germ oil, shea butter, coltsfoot colostrum, marker daisy nut oil, meadow home oil, egg oil, beef fat, , Jojoba oil, canned wax, carnauba wax, liquid lanolin or hardened castor oil, or the like, but is not limited thereto.

Specific examples of the moisturizing agent include water-soluble low-molecular moisturizing agents, oil-soluble molecular moisturizers, water-soluble polymers or oil-soluble polymers. Specific examples of the water-soluble low-molecular moisturizing agent include serine, glutamine, sorbitol, mannitol, sodium pyrrolidone-carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B Polypropylene glycol (polymerization degree n = 2 or more), polyglycerin B (polymerization degree n = 2 or more), lactic acid or lactic acid salt. Specific examples of the lipid-soluble low-molecular moisturizing agent include, but are not limited to, cholesterol or cholesterol ester. Specific examples of the water-soluble polymer include carboxyvinyl polymer, polyaspartic acid, tragacanth, xanthan gum, methylcellulose, hydroxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, carboxymethylcellulose, water-soluble chitin, chitosan or dextrin But is not limited thereto. Specific examples of the oil-soluble polymer include, but are not limited to, polyvinylpyrrolidone / eicosene copolymer, polyvinylpyrrolidone / hexadecene copolymer, nitrocellulose, dextrin fatty acid ester or polymeric silicone. Specific examples of the emollients include, but are not limited to, long chain acyl glutamic acid cholesteryl ester, cholesteryl hydroxystearate, 12-hydroxystearic acid, stearic acid, rosin acid or lanolin fatty acid cholesteryl ester.

Specific examples of the surfactant include nonionic surfactants, anionic surfactants, cationic surfactants and amphoteric surfactants. Specific examples of the nonionic surfactant include self-emulsifying monostearic acid glycerin, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, sorbitan fatty acid ester, POE (polyoxyethylene) sorbitan fatty acid ester, POE sorbit fatty acid ester , POE glycerin fatty acid ester, POE alkyl ether, POE fatty acid ester, POE hardened castor oil, POE castor oil, POE.POP (polyoxyethylene.polyoxypropylene) copolymer, POE.POP alkyl ether, polyether- We are not limited to acid alkanolamides, alkylamine oxides or hydrogenated soybean phospholipids. Specific examples of the anionic surfactant include fatty acid soap, alpha-acylsulfonate, alkylsulfonate, alkylarylsulfonate, alkylnaphthalenesulfonate, alkylsulfate, POE alkyl ether sulfate, alkylamide sulfate, alkyl phosphate, POE alkyl ginseng, Alkylamido phosphate, alkyloyl taurine salt, N-acyl amino acid salt, POE alkyl ether carboxylate, alkylsulfosuccinate, sodium alkylsulfoacetate, acylated hydrolyzed collagen peptide salt or perfluoroalkyl phosphate ester or It is not limited. Specific examples of the cationic surfactant include alkyl trimethyl ammonium chloride, stearyl trimethyl ammonium chloride, stearyl trimethyl ammonium bromide, cetostearyl trimethyl ammonium chloride, distearyl dimethyl ammonium chloride, stearyl dimethyl benzyl ammonium chloride, behenyl trimethyl ammonium bromide , Benzalkonium chloride, diethylaminoethylamide stearate, dimethylaminopropylamide stearate or lanolin derivative quaternary ammonium salts. Specific examples of the amphoteric surfactant include carboxybetaine, amidebetaine, sulfobetaine, hydroxysulfobetaine, amidosulfobetaine, phosphobetaine, aminocarboxylate, imidazoline derivative or amideamine And the like, but are not limited thereto.

Specific examples of the organic and inorganic pigments include inorganic pigments such as silicic acid, silicic acid anhydride, magnesium silicate, talc, sericite, mica, kaolin, Bengala, clay, bentonite, titanium mica, titanium oxide, bismuth oxychloride, zirconium oxide, Inorganic pigments such as titanium, aluminum oxide, calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, chromium oxide, chromium oxide, chromium, and combinations thereof; But are not limited to, polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluororesin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, Silk powder, cellulose, CI Pigment Yellow, CI Pigment Orange, and composite pigments of these inorganic pigments and organic pigments.

Specific examples of the organic powder include metallic soaps such as calcium stearate; Metal salts of alkyl phosphates such as sodium zinc cetylate, zinc laurylate and calcium lauryl laurate; Acylamino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc and N-lauroylglycine calcium; Amidosulfonic acid multivalent metal salts such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; Such as N-epsilon-lauroyl-L-lysine, N-epsilon-palmitoylidene, N-alpha-paratyylnitin, N-alpha-lauroyl arginine, N- Acyl basic amino acids; N-acylpolypeptides such as N-lauroylglycylglycine; Alpha-amino fatty acids such as alpha-aminocaprylic acid, alpha-aminoaurauric acid, and the like; Or polyethylene, polypropylene, nylon, polymethylmethacrylate, polystyrene, divinylbenzene-styrene copolymer, and tetrafluoroethylene.

Specific examples of the ultraviolet absorber include para-aminobenzoic acid, ethyl paranobenzoate, amyl paranobenzoate, octyl para-aminobenzoate, ethylene glycol salicylate, phenyl salicylate, salicylate oxalate, benzyl salicylate, butylphenyl salicylate, Menthyl benzyl, cinnamic acid benzyl, para-methoxy cinnamic acid-2-ethoxyethyl, paramethoxy methacrylate octyl, dipyrromethoxycinnamic acid mono-2-ethylhexane glyceryl, paramethoxy cinnamate isopropyl, diisopropyl diisopropyl cinnamate Ester mixtures, urocaninic acid, ethyl urocanoate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenone sulfonic acid and salts thereof, dihydroxymethoxybenzophenone, sodium dihydroxymethoxybenzophenone disulfonate, di Hydroxybenzophenone, tetrahydroxybenzophenone, 4-tert-butyl-4'-methoxydibenzoylmethane, 2,4,6-trianylino-p- (carbo-2'-ethylhexyl- Oxy) -1,3,5-tri But are not limited to, lyazin or 2- (2-hydroxy-5-methylphenyl) benzotriazole.

Specific examples of the bactericides include hinokitiol, trichloroacid, trichlorohydroxydiphenyl ether, crohexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, Benzalkonium, photosensitizer 301, mononitro and eicol sodium, or undecylenic acid.

Specific examples of the antioxidant include, but are not limited to, butylhydroxyanisole, gallic acid propyl, or erosorbic acid.

Specific examples of the pH adjuster include, but are not limited to, citric acid, sodium citrate, malic acid, sodium malate, fumaric acid, sodium fumarate, succinic acid, sodium succinate, sodium hydroxide or sodium monohydrogenphosphate.

Specific examples of the alcohol include higher alcohols such as cetyl alcohol and the like.

Any of the above components may be blended to the extent that the object and effect of the present invention are not impaired. Specifically, the blending component may be added in an amount of 0.01 to 5% by weight based on the total weight , And more specifically 0.01 to 3% by weight. However, the present invention is not limited thereto.

The cosmetic composition of the present invention may take the form of a solution, an emulsion or a viscous mixture, but is not limited thereto. The ingredients contained in the cosmetic composition of the present invention may contain, as an active ingredient, the ingredients commonly used in cosmetic compositions in addition to the mixed extracts of the present invention, for example, But are not limited to, conventional adjuvants and carriers such as flavoring agents.

The cosmetic composition of the present invention may be prepared in any formulations conventionally produced in the art and may be formulated into emulsion, cream, lotion, pack, foundation, lotion, essence or hair cosmetic, but not limited thereto. Specifically, the cosmetic composition of the present invention can be used as a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, a nutrition cream, a moisturizing cream, a hand cream, Packs, soaps, cleansing foams, cleansing lotions, cleansing creams, body lotions or body cleansers.

When the formulation of the present invention is a paste, a cream or a gel, animal fibers, plant fibers, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component But are not limited thereto.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether, but is not limited thereto.

When the formulation of the present invention is a solution or an emulsion, a solvent, a solvent or an emulsifier is used as a carrier component, and water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid esters of sorbitan can be used, but are not limited thereto.

When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, But are not limited to, cellulose, aluminum metahydroxide, bentonite, agar or tracant.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide But are not limited to, ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolenic derivatives or ethoxylated glycerol fatty acid esters.

The present invention provides a pharmaceutical composition for the prevention and treatment of skin diseases, which comprises a mixed extract of Pseudomonas sp.

In the above-mentioned extraction, water, alcohol or a mixture thereof is preferably used as an extraction solvent for the mixed extract of Porphyrae, Lycopersicon esculentum, Lycopersicon esculentum, and Rootgrass. The alcohol is preferably a C ₁ to C ₂ lower alcohol, and it is preferable to use a lower alcohol or ethanol or methanol. As the extraction method, it is preferable to use shaking extraction, Soxhlet extraction or reflux extraction, but it is not limited thereto. It is preferable that the extraction solvent is added by 1 to 10 times, preferably 4 to 6 times, more preferably by adding to the mixed amount of the above-mentioned extraction solvent, The extraction temperature is preferably 20 to 100 ° C, more preferably 20 to 40 ° C, most preferably room temperature, but is not limited thereto. The extraction time is preferably 10 to 48 hours, more preferably 15 to 30 hours, most preferably 24 hours, but is not limited thereto. The number of times of extraction is preferably 1 to 5 times, more preferably 3 to 4 times, more preferably 3 times, but is not limited thereto. In addition, the above-mentioned mixed extract is characterized in that it is extracted with a mixture of Pseudomonas aeruginosa, Pseudomonas sp., Lycopersicon esculentum, Lycopersicon esculentum, and Rootgrass. However, the respective extracts of Pseudomonas sp.

Further, in the above method, it is preferable to use a vacuum decompression concentrator or a vacuum rotary evaporator, but not limited thereto. The drying is preferably performed under reduced pressure, vacuum drying, boiling, spray drying or freeze drying, but not always limited thereto.

In addition, the mixture of Pseudomonas aeruginosa, Pseudomonas aeruginosa, Lycopersicon esculentum and Lycopersiconia fructus comprises 25 to 40 parts by weight of Pseudomonas aeruginosa, 25 to 40 parts by weight of Psoriasis, 10 to 20 parts by weight of licorice and 10 to 20 parts by weight of Rootini, More preferably 30 to 38 parts by weight of porky-yong, 30 to 38 parts by weight of psyllium seeds, 13 to 18 parts by weight of licorice and 13 to 18 parts by weight of rhizome, 32 to 35 parts by weight of glutinous rice, 15 to 17 parts by weight of licorice and 15 to 17 parts by weight of curl.

The skin disease is preferably, but not limited to, psoriasis, atopic dermatitis, systemic lupus erythematosis, contact dermatitis, allergic skin disease, acne, acne and urticaria.

In a specific embodiment of the present invention, the present inventors obtained an extract by pulverizing a mixture of Pseudomonas aeruginosa, Pseudomonas sp., Lycopersicon esculentum, Lycopersicon esculentum and Rootgrass, followed by hot water extraction using a mixed solvent of water and alcohol and lyophilization. When the obtained mixed extract was treated with SV-HEKx cells, cytotoxicity was not observed (see FIG. 1), and protein expression and promoter activity of inolluclease and lorikreen constituting keratinocyte membrane were increased to promote cell differentiation (See FIGS. 2, 3 and 4), it was confirmed that the growth of cells treated with the mixed extract was similar to that of calcium-treated positive control (see FIG. 5). In addition, when the mixed extract was treated with a mouse, it was confirmed that skin water loss was suppressed (see FIG. 6), and the expression of keratin-binding protein was increased (see FIG. 7). Accordingly, the mixed extract of Phellinus lachrymum, Pseudomonas sp., Lycopersicon esculentum, Lycopersicon esculentum and Lycopersicon esculentum of the present invention can be effectively used as a cosmetic composition for skin moisturization.

The pharmaceutical composition of the present invention may further comprise a pharmaceutically acceptable carrier. The term " pharmaceutically acceptable "as used herein means that the composition is free of toxicity to cells or humans exposed to the composition. Compositions comprising a pharmaceutically acceptable carrier can be of various oral or parenteral formulations. In the case of formulation, it can be prepared using diluents or excipients such as fillers, extenders, binders, humectants, disintegrants, surfactants and the like which are usually used. The carriers, excipients and diluents include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methylcellulose, microcrystalline cellulose, But may be at least one selected from the group consisting of polyvinylpyrrolidone, physiological saline, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil, dextrin, calcium carbonate, propylene glycol and liquid paraffin, But are not limited to, ordinary carriers, excipients or diluents. These components may be added to the mixed extracts of the active ingredients of Porphyra, Phyllostachys, Licorice, and Rootgrass, either independently or in combination.

Solid formulations for oral administration may include tablet pills, powders, granules, capsules and the like, which may contain one or more excipients, such as starch, calcium carbonate, sucrose or lactose, lactose, gelatin, and the like. In addition to simple excipients, lubricants such as magnesium stearate, talc, and the like may also be used. Liquid preparations for oral administration include suspensions, solutions, emulsions, syrups and the like. Various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included in addition to water and liquid paraffin, which are simple diluents commonly used. have. Formulations for parenteral administration may include sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Examples of the non-aqueous solvent and the suspending agent include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, and injectable ester such as ethyl oleate. As the base of the suppository, witepsol, macrogol, tween 61, cacao paper, laurin, glycerogelatin and the like can be used.

The pharmaceutical composition of the present invention may also be in the form of tablets, pills, powders, granules, capsules, suspensions, solutions, emulsions, syrups, sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, Or a pharmaceutically acceptable salt thereof.

The pharmaceutical composition of the present invention can be administered in a pharmaceutically effective amount. There is no particular restriction on the dosage, and it may vary depending on the body's absorption, body weight, age, sex, health condition, diet, administration time, administration method, excretion rate, severity of disease and the like. The pharmaceutical composition of the present invention is prepared in consideration of an effective dose range, and the unit dosage formulations thus formulated are classified according to the judgment of the expert who monitors or observes the administration of the drug, if necessary, Or may be administered several times at a predetermined time interval. Preferably, the pharmaceutical composition of the present invention is administered at a dose of 0.5 to 5000 mg / kg, preferably 50 to 500 mg / kg, more preferably 50 to 500 mg / kg, per day, based on the amount of the mixed extract of Porphyrae, mg / kg, which may be administered once a day, or divided into several doses.

In addition, the present invention provides a skin external preparation for skin moisturizing, which contains, as an active ingredient, a mixed extract of Pseudomonas sp.

It is preferable that the mixed extract of Phellodendron, Phellinus linteus, Lycopersicon esculentum, Lycopersicon esculentum and Lycopersicon esculentum of the present invention is used as a raw material for cosmetics, bath products, soaps, pharmaceutical ointments, veterinary medicines or packaged products. That is, the therapeutic agent for a skin disease containing the mixed extract of the present invention as an active ingredient is a solution, a suspension, a spray, a patch, a pad, a cream, an ointment, a gel, a tablet or a pill .

In addition to the mixed extracts of Corynebacterium glutinosa, Lactobacillus syrup, Lycopersicum ganoderma, and Lycopersicum, the solution may be prepared by mixing conventional excipients such as solvents, solubilizing agents and emulsifiers such as water, ethyl alcohol, isopropyl alcohol, ethyl carbonate, ethyl acetate, Soybean oil, aloe vera, glycerin, natural corn stover oil, olive oil, castor oil, almond oil and sesame oil, glycerol, glycerol, , Glycerol formal alcohol, tetrahydrofurfuryl alcohol, polyethylene glycol and fatty acid esters of sorbitan, or mixtures of these substances and include methyl or propyl-p-hydroxybenzoate or sorbic acid as preservative can do.

The suspensions may contain conventional excipients such as liquid diluents (e.g., water, ethyl alcohol and propylene glycol, polyethylene glycol), and suspending agents (e.g., ethoxylated isostearyl alcohol , Polyoxyethylene sorbitol, sorbitan esters, cellulose derivatives and preservatives for hydrogenation), microcrystalline cellulose, aluminum metahydroxide, bentonite, agar and tragacanth, injectable esters such as ethyl oleate, or mixtures of these substances ≪ / RTI >

The ointments, creams and gels may contain conventional excipients, such as animal and vegetable fats, wax paraffin, starch, targakan, cellulose derivatives, polyethylene glycol, silicone, bentonite, silicic acid , Talc, and zinc oxide, or a mixture of these materials.

In addition, the administration method can be generally applied to the affected part, and the preferable administration method can be selected according to the formulation. The daily dose of the composition of the present invention is determined depending on the subject to be administered, the method of administration, and symptoms, but generally, the mixed extract of Poeffopoulos, Rhodiola, Licorice, and Rootini, which are contained in the composition, is administered in an amount of 0.01 to 100 占 퐂 / . The number of times of administration is preferably two times a day or more, but the number of times of administration can also be adjusted depending on the symptom level. However, the dosage needs to be varied and can be varied, in particular considering the sperm specificity and weight of the object to be treated, the type and depth of the disease, the nature of the formulation, the nature of the drug administration, and the duration or interval of administration.

In order to effectively treat skin diseases, it is important to select a suitable external preparation. In order to treat a skin disease, a mixed extract of Poeffea, Persimmon, Lycopersicum, and Rootgrass may be appropriately diluted and applied directly to the skin. In the present invention, an ointment agent can be effectively applied to the skin treatment agent. The ointment of the present invention is prepared by combining the above-described skin disease therapeutic agent with an inorganic substance and then coating it with a liposoluble base. The inorganic material is preferably a material having excellent antimicrobial activity, anti-inflammatory effect, epidermal regeneration effect, etc. Specific examples thereof include zinc oxide, zinc carbonate, iron oxide and the like.

In addition, it is preferable to further use a ceramic carrier capable of safely impregnating the skin disease therapeutic agent of the present invention. As the ceramic carrier, zeolite, talc, gypsum, mortar and mixtures thereof are preferably used. Such a ceramic carrier is excellent in the impregnation property of the water-soluble component, so that the water-soluble component can be smoothly supplied to the skin.

Hereinafter, the present invention will be described in detail with reference to Examples and Experimental Examples.

However, the following Examples and Experimental Examples are merely illustrative of the present invention, and the content of the present invention is not limited by the following Examples and Experimental Examples.

< Example  1> Preparation of mixed crude drug extract

The species of Taraxacum platycarpum H. Dahlstedt, Heartleaf Houttuynia , Glycyrrhiza uralensis Fischer and root bark of Ulmus davidiana ) was prepared by the following method.

Specifically, all medicinal plants were purchased from Omniherb (Daegu, Korea). 80 g of Porphyry, 80 g of Rhus incense, 40 g of licorice and 40 g of Root skin of Rootgrass were pulverized and extracted with 8 times of ethanol. The mixed extract was filtered using filter paper (Whatman paper), distilled under reduced pressure and freeze-dried (Eyela, Irvine, CA).

< Experimental Example  1> Cytotoxicity test of mixed herbal medicine extract

To examine the cytotoxicity of the crude herbal medicine extract prepared in Example 1, SV-HEKs cells were cultured and MTT assay was performed.

Specifically, SV-HEKs (Simian virus 10 large T antigen-transformed human epidermal keratinocytes) cells were cultured in K-SFM medium supplemented with bovine pituitary extract (BPE) and recombinant human epidermal growth factor (EGF) -serum free media. The cells were cultured in a 12-well culture plate at a concentration of 2 × 10 ⁵ for 12 hours. Then, the mixed herbal medicine extract prepared in Example 1 was dissolved in DMSO, Lt; / RTI &gt; Then, 2 mg / ml MTT solution was added and reacted for 2 hours, and the absorbance was measured at 540 nm with an ELISA reader.

As a result, as shown in Fig. 1, it was confirmed that the mixed crude herbal medicine extract including Pseudomonas aeruginosa, Pseudomonas aeruginosa, Lycopersicon esculentum, and Rootgrass did not show cytotoxicity even at a concentration of 10 μg / ml (FIG. 1).

< Experimental Example  2> Measurement of cell differentiation by mixed crude drug extract

<2-1> Confirmation of protein expression by mixed herbal medicine extract

In order to confirm the effect of the mixed herbal medicine extract of the present invention on the keratinocyte differentiation, the cell differentiation of the proteins involucrin keratinocyte (involucrin protein and loricrin protein) constituting keratinocytes was measured. At this time, calcium (Ca ^{2 +} ), which is known as a cell differentiation promoter, was used as a positive control.

Specifically, the mixed herbal medicine extract prepared in Example 1 was dissolved in DMSO and treated with 10 μg / ml of SV-HEKs cells and cultured for 3 days or 7 days. After the cultured cells were dissolved in propre solution (Intron, Korea), the total protein amount was quantified using BCA protein assay reagent (Pierce Biotechnology, Rockford, IL). SDS-PAGE was developed to perform western blotting, and after development, the cells were transferred to nitrocellulose membranes and the following primary antibodies were reacted at a dilution of 1: 1000: Inbolucurin (Cat # sc-21748 (Cat # sc-133757, Santa Cruz Biotechnologies, Santa Cruz, CA), filaggrin (Cat # sc-30229, Santa Cruz Biotechnologies, Santa Cruz, Calif.), And actin (Cat # A2668, Sigma, St Louis, MO). Secondary antibody conjugated with peroxidase was used as the secondary antibody.

As a result, as shown in Fig. 2, the expression of inolluclease and lorikreen increased significantly in the cells treated with the mixed herbal medicine extract for 3 days. In addition, the expression of pilagrin as a late differentiation marker in the cells treated with the mixed herbal medicine extract for 7 days was greatly increased (FIG. 2), confirming that the mixed herbal medicine extract improved the keratinocyte differentiation.

<2-2> Expression of Promoter by Mixed Herb Extract

As a result of the above <Example 4>, it was confirmed that the expressions of the proteins constituting the keratinocytes of the skin, borculin and loricrin, were increased in the cells treated with the mixed herbal medicine. Thus, a luciferase reporter assay was performed to confirm whether the protein expression was carried out in the DNA transcription step.

Specifically, inulin-luc reporter adenovirus (Ad / lnv-luc) and loricrin-luc reporter adenovirus (Ad / lor-luc) GDNA was isolated from keratinocytes and used as a PCR template. The sequences of the primers are shown in Table 1 below.

SEQ ID NO: designation The sequence (5'-3 ') One The phosphorylation promoter F CTCCATGTGTCATGGGATATG 2 Inboluclene promoter R TCAACCTGAAAGACAGAAGAG 3 Lorocreen promoter F TACCAAGCAATCCTCTCACCTTGG 4 Rorikreen promoter R TGAGGAGAGAAGATGCTGGC

Each PCR product contained +1,239 bp (base pair) from inbolucurin transcription site -2,467 and +12 bp from lorikreen transcription site -2,033. The above-mentioned promoter PCR product was cloned into pGL3 vector, pENTR vector to complete the reporter adenovirus.

SV-HEKs cells were grown on a 12-well culture plate to about 50%, and then the reporter adenovirus was transduced. After the transfection, the cell culture medium was filled with a new culture medium, and the mixed herbal medicine extract prepared in Example 1 was dissolved in DMSO and treated with 10 μg / ml for 48 hours. After 48 hours, cell extracts were obtained using a cell lysis buffer and luciferase reporter assay Promega, Medison, WI) according to the protocol of the manufacturer.

As a result, as shown in FIG. 3 and FIG. 4, it was confirmed that the promoter activity of inbolucurin and lorikreen increased in cells treated with the mixed herbal medicine extract (FIGS. 3 and 4). These results indicate that the mixed herbal extract enhances the differentiation of keratinocytes.

< Experimental Example  3> Measurement of cell proliferation by mixed herbal medicine extract

The keratinocyte differentiation accelerates the arrest of the cell cycle. In order to examine the effect of the herbal extract of the present invention on the cell growth, one of the precursors of DNA, thymidine ) thymidine uptake assays ^{([3 H] thymidine uptake assay} ) by adding the tagged isotopes ⁽³ H) as a marker for cells that measure the amount of isotope in the absorption was conducted.

Specifically, SV-HEKs cells were cultured on a 60 mm culture plate, and then 1 μCi of [ ³ H] thymidine (Amersham, Buckinghamshire, UK) and the mixed herbal extract prepared in Example 1 were dissolved in DMSO to give 10 Mu] g / ml. After 1, 3, and 5 days of culture, the cells were washed twice with PBS, and the cells were incubated at room temperature with 0.1 N sodium hydroxide (NaOH). After cell lysis, radioactivity was measured using a liquid scintillation counter.

As a result, as shown in FIG. 5, it was confirmed that the group treated with the crude herbal medicine extract increased the differentiation of the cells by keeping the cell cycle of the keratinocytes at a resting state similarly to the group treated with calcium (FIG. 5).

< Experimental Example  4> Measurement of skin function recovery by mixed herbal medicine extract

Measurement of transepidermal water loss is well known as a measure of the degree of damage to skin barrier function. To examine the effect of the mixed herbal medicine extract of the present invention on the skin barrier function, skin moisture loss measurement and skin cross-section observation using immunostaining were performed.

Specifically, 7-week-old mice (hairless mouse, Orient Bio Inc., Seongnam, Korea) were divided into skin surface sections using a tape. The mixed herbal medicine extract prepared in Example 1 was dissolved in 70% polyethylene glycol and 30% ethanol, and 100 쨉 g / ml was applied to the skin surface according to the divided skin surface sections, (Tewameter TM210, Germany) was used to measure skin moisture loss.

For immunological staining, a mouse model of skin moisture loss was dissected, a skin tissue sample was obtained, fixed in 10% formalin, and cut into paraffin. The sectioned paraffin section was again waxed, rehydrated, washed three times with PBS, and then proteinase K was added thereto for reaction at 37 ° C for 5 minutes. After the proteinase K reaction, the paraffin sections were treated with hydrogen peroxide (H ₂ O ₂ ) at room temperature for 10 minutes, and then incubated with 0.1% tween-20 and 1% bovine serum albumin Pretreated with PBS for 30 minutes, and then reacted with anti-mouse-pillar green antibody for 1 hour. The paraffin section was then reacted with a secondary antibody conjugated with peroxidase and then confirmed using a Chemmate envision (Dako, Carpinteria, CA) detection kit.

As a result, as shown in FIG. 6, the group treated with the mixed herbal extract had a faster recovery ability against moisture loss of the skin than the control group (FIG. 6). In addition, when the expression of the keratin-binding protein filaggrin in the skin tissue was confirmed using the immunostaining method, the expression of filla greens in the skin epidermis of the mouse was increased when the mixed herbal extract was applied (Fig. 7).

<110> Chungnam National University - Industry Collaboration Foundation <120> Cosmetic composition for skin moisturizing comprising the extract          of Taraxacum platycarpum H. Dahlstedt, Heartleaf Houttuynia,          Glycyrrhiza uralensis Fischer, root back of Ulmus davidiana <130> 2015P-03-017 <160> 4 <170> Kopatentin 2.0 <210> 1 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> IC_F <400> 1 ctccatgtgt catgggatat g 21 <210> 2 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> IC_R <400> 2 tcaacctgaa agacagaaga g 21 <210> 3 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> LC_F <400> 3 taccaagcaa tcctctcacc ttgg 24 <210> 4 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> LC_R <400> 4 tgaggagaga agatgctggc 20

Claims (8)

Taraxacum platycarpum H. dahlstedt), Heartleaf Houttuynia , licorice ( Glycyrrhiza The present invention relates to a cosmetic composition for skin moisturisation, which comprises as an active ingredient, a mixed extract of uralensis Fischer and root bark of Ulmus davidiana .
The cosmetic composition for improving skin moisturization according to claim 1, wherein the mixed extract is extracted with water or a C ₁ to C ₂ lower alcohol or a mixture thereof as a solvent.
[5] The composition according to claim 1, wherein the mixture comprises 25 to 40 parts by weight of perfluoro-olefins, 25 to 40 parts by weight of perlite, 10 to 20 parts by weight of licorice, and 10 to 20 parts by weight of rhizome, A cosmetic composition for skin moisturization improvement.
The cosmetic composition for improving skin moisturization according to claim 1, wherein the composition promotes keratinocyte differentiation.
A pharmaceutical composition for the prevention and treatment of skin diseases, which comprises as an active ingredient, a mixed extract of Pseudomonas sp.
[Claim 6] The composition according to claim 5, wherein the mixture comprises 25-40 parts by weight of perfluoro-olefins, 25-40 parts by weight of rhizome, 10-20 parts by weight of licorice and 10-20 parts by weight of rhizome A pharmaceutical composition for prevention and treatment of skin diseases.
[Claim 7] The pharmaceutical composition according to claim 5, wherein the skin disease is psoriasis or atopic dermatitis.
A skin external preparation for skin moisturizing, which contains a mixed extract of Pongyeonggong, Hwasungcho, Licorice, and Rootbeop as effective ingredients.

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