KR19980069516A - Extract and skin cosmetic containing same - Google Patents

Abstract

The present invention relates to a thin extract and a skin cosmetic containing the same, the water extract of water powder treatment, 60-95% ethanol extract of thin powder excellent in safety and prevention of skin damage and improvement effect, whitening effect, antioxidant effect, etc. As a thin extract extracted with a mixture of 1,3-butylene glycol and water, it is applicable as a component of skin cosmetics.

Description

Extract and skin cosmetic containing same

The present invention relates to a skin cosmetic containing the extract of the same, in particular, it is excellent in the skin damage prevention and improvement effect, whitening effect, antioxidant effect, and also excellent in terms of safety, water extract treatment water extract of gourd, which is applicable as a component of skin cosmetic, It relates to a skin extract extracted with a mixture of 60 ~ 95% ethanol extract, 1,3-butylene glycol (1,3-butylene glycol) and water and skin cosmetics containing the same.

General external skin preparations include cosmetics, medicines, and quasi-drugs. Especially, external skin preparations for the purpose of preventing and improving skin damage have been conventionally used in combination with various raw materials including raw materials extracted from natural products. For example, ginseng extract, yeast extract, extracts such as pine mushroom extract, natural polymers such as collagen, skin rejuvenating agents such as amino acids and vitamins, or moisturizing agents such as glycerin and 1,3-butylene glycol are used to prevent skin damage. And it has been used in skin cosmetics for the purpose of improvement.

Park is native to Africa or tropical Asia, and is commonly used in farms all over Korea. It is a vine grass that climbs on the roof. The fruit of this vine is called Park. Park's scientific name is Lagenaria leucantha RUSBY, and there are various genus names. Ho, Ho, Ho, Pho, Po, Horo, Hoseo, Hoja (포 子), poins (匏 瓜), bakahji, horobak, reference longbak, bakkoji, baksok, dongja (冬瓜 子), dongwa seeds are also called. It is characterized as a yearly vine of cucumber family, naturalized plant (Kim Tae-jung, Korea Resource Plant IV, Seoul National University Press, 1996).

The gourd generally extends about 5m long, with short hairs all over, and wrapped with tendrils. Flowers bloom in July-September. Flowers are white and bloom in the evening or at dusk. It is 5 ~ 10cm in diameter and divided into 5 pieces. In October, berry (漿果: flesh and water, fruit with a lot of seeds in the middle) is a berry is more than 30cm in diameter, and when it is ripe, the outer shell becomes hard. The inside of the gourd was eaten or thinly divided into medicine. In addition, the horo may be chopped into small pieces to make oil, and the ripe skin may be used as a container.In Korea and Manchuria, it is said to be grown around the house (Kim Tae-jung, Sanyacho of Korea, Kookil Media, 1996).

There are two kinds of gourds: small and large, long fruit and round. In Korea, it is said that the round ones are made with fresh or soft leaves and soft skins with fresh herbs or finely cut to make bakkoji and steamed in a pot to eat juice. In addition, he divided pieces into pieces and contained objects. These were called “bakes” or “bakes”. It is also used as a storage container for grains, and it is reported that the Chinese used the outer shell for food and were used for making beans or noodles.

The seeds were also called hoja or confectionery, western and southern families. Seeds were widely used as snacks by Chinese as well as Koreans. The gourd was cut in half and used as a device for drinking or containing water. It is introduced as a checker (水瓢 子). It is known that gourd is used for edible, ornamental and industrial purposes, and the endothelial of fruit is used for edible and is used for ornamental vinegar and crafts.

The efficacy of gourd has been known for many years, especially diuretic, whooping cough, poisoning, and freckles. In addition to folk remedies ① edible, industrial, ornamental, medicinal is used. Dried fruit bark mixed with licorice is good for pertussis or coughing. You can also add bamboo leaves to the fruit bark for sweetening. ② Dried or dried flesh of the seeds or dried flesh to eat is effective in diuresis (利尿) and can be good for species, each, hemorrhoids, irregular menstruation. ③ Fish, crabs, mushrooms addiction to the skin of the fruit may be drunk or drink the salty juice of the fruit. ④ chop the fruit into freckles, strain with water and alcohol with seeds to filter this. Scoop out the juice in half and apply it to your face or freckles over long periods of time. You can also finely chop the seeds, mix the same amount of peach blossom and knead it with honey. ⑤ in case of hemorrhoids, wash the affected part with a single juice. In addition, children's diarrhea may crush fruit and drink the juice.

However, despite the efficacy of folklore, there is virtually no research on Park in Korea.

It is an object of the present invention to provide a skin cosmetic containing the same extract that is excellent in safety and excellent in preventing and improving various skin damages. That is, to provide a skin extract that is applicable to skin cosmetics and exhibits excellent whitening effect, antioxidant effect, etc. when contained in cosmetics.

The extract of the present invention for achieving the above object is an extract of a powdered water-treated water extract, 60 ~ 95% ethanol extract or a mixture of 1,3-butylene glycol and water. In addition, the thin extract is applicable as a component of the skin cosmetic, wherein the content of the thin extract is preferably 0.05 to 20% by weight.

In the present invention, the effect that can be used in cosmetics in the water extract of the water-treated water extract of Park, cultivated in Korea, 60-95% ethanol extract, 1,3-butylene glycol and water mixture is proved and contained in cosmetics Excellent antioxidant effect, whitening effect, etc. have been found to complete the present invention.

First, the whitening effect and antioxidant effect were investigated to confirm the applicability of skin extracts to skin cosmetics. Park used field cultivation in Numseong-si, Chungbuk, Korea. To use it as a sample, it was taken and sliced a fully ripe gourd around September, and the seeds and other parts of the park fruit were separated and the remaining parts except seeds were used as samples. Part is called gourd). The foil was dried at room temperature until there was no change in weight, then placed in a nitrogen tank and frozen, and ground in a mortar to obtain a powder. The water extract of the powdered water, the water extract extracted with a mixture of 60-95% ethanol extract, 1,3-butylene glycol and water was used as a sample.

The effect of free radical scavenging was examined as an effect test for application to skin cosmetic. As a result, it showed the highest free radical scavenging effect at 3% by weight sample concentration. The sample concentration (SC ₅₀ ) required to reduce 50% of the 1,1-diphenyl-2-picrylhydrazyl (DPPH) concentration was 0.4% by weight and was superior to any other material in effect.

In the antioxidant effect test using linoleic acid automatic oxidation, Park ethanol extract showed superior antioxidant effect (44%) compared to vitamin E (42.3%), which was superior to the control group.

The whitening effect on the extracts was examined by experiments of tyrosinase activity inhibitory activity, melanin production inhibition of Strepthmyces bikiniensis, and melanin production inhibition of B16-F1 melanoma cells. As a result, the concentration (IC ₅₀ ) that inhibits tyrosinase activity by 50% was 0.2% or less, and the melanin-producing region was inhibited by 20 mm at a concentration of 0.005%. In addition, the minimum concentration of inhibiting melanin production of B16-F1 melanoma cells was 0.1%, showing an excellent effect as an external preparation for skin, especially skin cosmetics.

Examples of application as skin cosmetics containing the extract of the present invention have not been reported to date. The amount of this extract to the skin cosmetic was not limited, but 0.05 to 20% by weight was found to be suitable, and the effect was not expected at too small content, and the effect was not greatly increased even if blended more than too much.

In addition, the extract of the present invention is easily mixed with water and difficult to blend with fats and oils and organic solvents, but can be blended into the skin cosmetic even in a suspended state and has no effect on the effect. The external skin preparation of the present invention is suitable to be used as a skin cosmetic, and common ingredients such as oil, water, surfactants, moisturizers, lower alcohols, thickeners, chelating agents, pigments, preservatives, fragrances, etc. It is possible to combine them as necessary. In the present invention, the skin cosmetic is used for the skin, for example, a cosmetic, such as a lotion, milky lotion, cream, pack, and the application in particular to the whitening cosmetics.

Hereinafter, the present invention will be described in more detail with reference to Examples.

These examples are merely illustrative of the present invention and the scope of the present invention is not limited to these examples.

Example 1

Park (Lagenaria leucantha RUSBY) used the reference long bak, gourd, etc., which was cultivated in the voice of Chungbuk, Korea. The remaining part was used as a sample. The foil was dried at room temperature until there was no change in weight, and then placed in a liquid nitrogen tank to freeze and crumbled.

Three solvents were used for solvent extraction. The first is distilled water, the second is ethanol, and the third is a mixture of 1,3-butylene glycol and water.

Extraction by water was put into 10 ~ 30% by weight of the powder powder in water, and water-bathed at 40 ~ 80 ℃ 48 ~ 100 hours and filtered through a mesh mesh of 400 mesh size to separate the foil and filtrate. Subsequently, the separated filtrate was sterilized by 0.45 μm filter paper and used as a sample. When the powder powder is used in less than 10% by weight, the efficacy of the extract, the effect is reduced, and when used in excess of 30% by weight, there is a problem in extracting the powder powder is not submerged in the liquid. In addition, even if the water bath treatment is performed for 100 hours or more, no further effect is exhibited.

Extraction by ethanol is added in 10 ~ 30% by weight in 60%, 80%, 95% ethanol solution in water and soaked in 25 ~ 60 ℃ for 15 ~ 60 days, but the temperature is circulated every day. The effect of the extract was higher than fixation at temperature. After this process, the extract was filtered through a mesh of 400 mesh size to separate the gourd and the filtrate, and the separated filtrate was filtered with 0.45 μm filter paper and used as a sample.

In the extraction by using a mixture of 1,3-butylene glycol and water, the ratio of 1,3-butylene glycol and water is mixed in a weight ratio of 8: 2 to 4: 6, and the mixture is mixed with 10 to 30% by weight. After extraction, the mixture was extracted with a water bath at 40-80 ° C. for 8-48 hours, filtered through a mesh mesh of 400 mesh size to separate the gourd and the filtrate, and the filtrate was filtered with 0.45 μm filter paper to be used as a sample.

Example 2

In this example, the effect of free radical scavenging was observed.

0.2mM 1,1-diphenyl-2-picrylhydrazyl (1,1-diphenyl-2-picrylhydrazyl, DPPH) Methanol solution in 0.5 ml of various concentrations of water extract, ethanol extract, 1,3-butyl 1 ml of 10% ethanol solution of each extract extracted with a mixture of len glycol and water was added and mixed, and then left for 10 minutes at 37 ° C. and then the absorbance was decreased at 517 mm. As a result, the free radical scavenging ability and the concentration (SC ₅₀ ) which reduces the free radical 50% were calculated as follows. As a result, the maximum performance was shown in about 3% by weight of the sample and SC ₅₀ was 0.4% by weight (Table 1). This has a superior effect than any other material.

* Free radical inhibition rate (%) = [1- (E-B) / C] × 100

(B: control group, C: reference group, E: sample group)

* Scavenging ability (SC ₅₀ ): The size of the activity is expressed as the concentration of the sample required to reduce the 1,1-diphenyl-2-picrylhydrazyl (66.7㎛) concentration by 50%.

TABLE 1

Example 3

This embodiment compares the degree to which the automatic oxidation is suppressed by using linoleic acid, which is a good automatic oxidation material. 3 ml of 10 mM linoleic acid solution and 0.075 ml of the sample adjusted to 4 mg / ml are added to an 8 ml cap tube, and the mixture is left at 4 ° C. After 0.1 ml of the mixed solution was added to 4.7 ml of 75% aqueous ethanol solution after 24 hours, 0.1 ml of 30% ammonium thiocyanate solution and 0.1 ml of ferrous chloride reagent were added and left for 3 minutes, followed by color development. Absorption wavelength was measured at, and the antioxidant effect was measured as a percentage in comparison with the control group. In the antioxidant effect test using linoleic acid automatic oxidation, the extract extracted by Example 1 was found to have an excellent antioxidant effect (44%) compared to vitamin E (42.3%) because the antioxidant effect is superior to the control (Table 2).

TABLE 2

Example 4

This embodiment is to determine the whitening effect by looking at the degree of functional inhibition of the enzyme tyrosinase (tyrosinase) to determine the whitening effect of the extract extracted by Example 1.

Tyrosinase is an enzyme that accelerates the oxidation of tyrosine in vivo and helps melanin production. In this example, a method of inhibiting the function of this enzyme to inhibit the oxidation of tyrosine to form a black polymer called melanin (Pomerantz SH, J. Biochem., 24, 161-168 (1996)) Application was judged as a whitening effect.

Morus extract (stock solution, v / v% of distilled water of 10, 1, 0.1, 0.01), gourd extract (stock solution, v / v% of distilled water of 0.2, 0.05, 0.005, 0.0005), oil-soluble licorice extract, kojic acid, Arbutin and hydroquinone (v / v% of distilled water of 1, 0.1, 0.01, 0.0001, 0.0001) were used as samples to investigate the inhibitory effect of tyrosinase activity.

For inhibitory activity against tyrosinase, 15 μl of the sample was placed in a microplate (96 well), 150 μl of 0.1 M phosphate buffer (pH6.86) and 25 μl of 1.5 mM L-tyrosine solution were added, followed by 2,380 units / ml of mushroom tyrosinase ( Sigma, USA) (0.05M phosphate buffer, pH6.86) was added to the reaction for 10 minutes at 30 ℃ was measured at 490nm using a microplate. Inhibition rate for tyrosinase (%) was calculated by the following equation, IC ₅₀ value is the concentration of the inhibitor reaches 50% enzyme inhibition rate (Table 3).

% Inhibition = {[(D-C)-(B-A)] / (D-C)} × 100

A: absorbance before reaction of the inhibitor

B: absorbance after the reaction of the inhibitor

C: Absorbance before reaction of adding no inhibitor

D: Absorbance after reaction of not adding inhibitor

The test results of tyrosinase activity inhibition showed that the IC ₅₀ value of the extract was less than 0.2%. Numerically, the inhibitory activity was found to be weaker than other substances, but it is considered to have an excellent effect considering that the extract is not a purely purified substance such as kojic acid or hydroquinone.

TABLE 3

Example 5

This example was used to measure melanin production inhibitory action against actinomycetes (Strepthomyces bikiniensis NRRL B-1049) was used (KCTC 9172) received from the Korea Institute of Science and Technology (KCTC).

Actinomycetes were spores cultured in papavizas' VDYA agar slant medium (V-8 juice 200ml, glucose 2g, yeast extract 2g, CaCO ₃ 1g agar 20g, distilled water 800ml, pH7.2) at 28 ° C for 2 weeks. The spore suspension was then made with sterile water. 0.2% yeast extract was added to ISP No. 7 0.2 ml each of the spore suspension was applied to the plate medium, and then the amount of the sample was applied to a paper disk moistened with the sample on the surface of the medium with 30 µg / paper disk and incubated at 28 ° C. The inhibition of melanogenesis was observed using the 4-hydroxyanisole known as the melanogenesis inhibitor as a control for the size of the melanogenesis inhibitors produced after 48 hours of incubation (Table 4).

Melanogenesis inhibitory activity against actinomycetes of each extract of gourd showed melanin production inhibition rate of 20 mm in the case of gourd ethanol extract. This inhibitory activity showed very good activity similar to 4-hydroxyanisole, a potent melanogenesis inhibitor.

TABLE 4

Example 6

This example was observed at the laboratory level to determine the skin whitening effect of the extract extracted with water bath treatment water extract, ethanol extract, 1,3-butylene glycol and a mixture of water.

B16 melanoma cells used in this example are cell strains derived from mice and cells that secrete a black pigment called melanin. Samples were treated during the artificial culture of these cells to evaluate the degree of melanin black pigment reduction. The B16-F1 melanoma cells used in this example were used by being sold from the American Type Culture Collection (Accession No .: 6323).

The inhibition of melanin biosynthesis of B16-F1 melanoma cells was determined as follows. B16-F1 melanoma cells were suspended in DMEM (Dulbecco's Modified Eagle's Medium (Gibco, USA)) containing 10% fetal bovine serum at a concentration of 5 × 10 ³ cells / ml. 5 ml of suspension cells were added to a tissue culture flask, and 0.1% of the assay sample was added thereto, followed by incubation at 37 ° C under 5% carbon dioxide-95% air condition. After incubation for 4 days, the melanogenesis was first determined by the color of the culture medium, the cells were washed with phosphate buffer, and then trypsinized to separate the cells from the flask. The cells were collected in a tube, washed with phosphate buffer, and centrifuged to compare the amount of melanin produced by the whitening agent with the amount of melanin produced by the whitening agent. The evaluation method was evaluated by visual observation of the degree of color change according to the treatment of nothing in the cell culture and the degree of black melanin pigment secretion when each sample was treated. As a result, the extract was found to effectively inhibit melanin pigment secretion (Table 5).

TABLE 5

[Example 7, 8, 9]

In the present Example, the comparative experiment of the cosmetics containing each thin extract extracted by Example 1 was done.

The composition of the cream used in the comparative experiment is shown in Table 6. First, the phase b) recorded in Table 6 is heated and stored at 70 ° C. To this, a) phase was added, and after pre-emulsification, it was emulsified uniformly with a homomixer, and it cooled slowly, and the cream (Examples 7-9, a comparative example) was prepared.

Each cream of Table 6 was applied to the right side of five subjects (20-35 year old female) for 2 consecutive days for 1 month. After completion of the experiment, the skins of the applied areas on both sides of the face were compared by using an image analyzer, and the darkest color was set as 5 medium color and the brightest color as 1, and the intermediate degree was estimated. Table 7 compares the facial skin color of the experimenter using the cream of the composition of Example 8 with the facial use area of the experimenter using the cream of the composition of the comparative example. As shown in Table 7, it can be seen that the whitening effect appeared on the face of the experimenter applying the cream containing the thin extract.

TABLE 6

TABLE 7

Other examples are shown below. That is, as described above, it showed an excellent effect on skin improvement and a skin beauty effect.

Example 10

To 8 g of 95% ethanol, 0.05 g of polypyrrolidone, 0.1 g of oleyl alcohol, 0.2 g of polyoxyethylene monooleate, 0.2 g of fragrance, 0.1 g of paraoxybenzoic acid methyl ester, a small amount of antioxidant, and a small amount of pigment are dissolved. 0.05 g of the extract extract of Example 1 and 5 g of glycerin were dissolved in 85.33 g of purified water, and the mixed solution was stirred and added to obtain a lotion having a skin improvement effect.

Example 11

1.2 g of cetyl alcohol, 10 g of squalane, 2 g of vaseline, 0.2 g of paraoxybenzoic acid ethyl ester, 1 g of glycerin monoesteralide, 1 g of polyoxyethylene (20 mole added) monooleate and 0.1 g of fragrance were mixed and dissolved at 70 ° C. 0.5 g of the thin extract of Example 1, 5 g of dipropylene glycol, 2 g of polyethylene glycol 1500, 0.2 g of triethanolamine, and 76.2 g of purified water were heated to 75 DEG C and dissolved. Both of them are emulsified by mixing and then cooled to obtain an emulsion having a water / oil-based skin improvement effect.

Example 12

5 g of 95% ethyl alcohol, 1.2 g of polyoxyethylene sorbitan monooleate, 0.3 g of kitulose, 0.2 g of hyaluronic acid, 0.2 g of vitamin E acetate, 0.2 g of sodium licorice, 0.1 g of paraoxybenzoic acid ethyl ester, 1 g of the thin extract of Example 1 and a suitable amount of pigment are mixed to obtain a cosmetic liquid having an effect of improving the skin.

The extract according to the present invention is excellent in preventing and improving various skin damages and is very excellent in terms of safety. In addition, the above-mentioned thin extract is applicable to skin cosmetics and exhibits excellent whitening and antioxidant effects when contained in cosmetics.

Claims (6)

The extract of the extract extracted with powdered water. The extract of the powder extracted with 60-95% ethanol against water. The extract of the powder extracted with a mixture of 1,3-butylene glycol and water. The extract of claim 3, wherein the mixing ratio of 1,3-butylene glycol and water is 8: 2 to 4: 6, and the extract is heated and extracted at 40 to 80 ° C. A skin cosmetic containing the extract of any one of Claims 1-4. The skin cosmetic according to claim 5, wherein the skin extract contains 0.05 to 20% by weight.