KR19990081226A - Skin cosmetic using natural mushrooms - Google Patents

Abstract

The present invention relates to a skin cosmetic using natural situation mushroom, hot water extract of natural situation mushroom, 20-40% ethanol aqueous extract, 1,3-butyl skin improvement effect, particularly skin whitening and antioxidant effect, excellent safety Natural situation mushroom extract extracted with a mixture of len glycol, propylene glycol and water is applicable as a component of skin cosmetics.

Description

Skin cosmetic using natural mushrooms

The present invention relates to skin cosmetics using natural situation mushrooms, in particular, the skin improvement effect such as skin whitening effect, antioxidant effect, as a component of skin cosmetics, hot water extract of natural situation mushrooms, ethanol extract, 1,3-butylene glycol, It relates to a skin cosmetic containing an extract extracted with a mixture of propylene glycol and water.

In general external skin preparations, there are cosmetics, medicines, quasi-drugs, etc. In particular, external skin preparations for the purpose of preventing and improving skin damage have conventionally used various raw materials starting from raw materials extracted from natural products. For example, ginseng extract, licorice extract, extracts such as pine mushroom extract, natural polymers such as collagen, skin cell activators such as amino acids and vitamins, or moisturizing agents such as glycerin and 1,3-butylene glycol are used to prevent skin damage. And it has been used in skin cosmetics for the purpose of improvement. However, in the use of materials that have been used for preventing and improving such conventional skin damage, it is collected in nature and artificially cultivated. It is well known that the same substance has different efficacy effects depending on the time of collection and the place of collection. (Proc, Jpn, Assoc, Mycotoxical, No. 30,1989). In particular, in the case of plants, in order to obtain specific effects such as skin whitening effect, immune resiliency effect, and antioxidant effect, there was a problem that the effects vary greatly depending on the harvesting site, collection time, and collection location of the plant.

Phellinus linteus (Ikekawa, T.,: Antitumor action of some basidiemycetes, especially Phellinus Linteus. GANN, 59: 155, l968) has been introduced since ancient times as a parasitic to mulberry trees in China. It is also found to be wild in hardwoods (Cha Dong-ryul, Monthly subp. P34-37,95.12). In Korea, the situation mushroom refers to the woody mud mushroom (phellinus linteus) and belongs to the pine scale Hymenochaetaceae. About 48 species of mud mushrooms belonging to this situation are reported around the world, but in Korea, rust mud mushrooms (P. ferruginosus), dried mud mushrooms (P. gilvus), worm mud mushrooms (P. igniarius), and branch mud mushrooms ( Eight species were collected from P. laevigatus, P. pomaceus, P. robustus, fir mud, and deciduous mushroom (P. pini). It has been used for the treatment of gynecological diseases such as uterine bleeding and menstrual irregularities under the names of differentiation, cotyledon, and Hawthorne. In the experiment, tumor suppression rate is 60-97% and is known to have an excellent effect.

As such, it is well known that a situation that grows naturally in nature has anti-tumor, but it is difficult to breed in nature and grows in an old tree of mulberry, which is called a real situation, and it is difficult to obtain with the decrease of mulberry itself. I grow it. The stems, leaves, and roots of mulberry trees have been used in medicine and cosmetics fields for a long time. Recently, only studies on the identification of anticancer effects with artificially cultivated situation mushrooms have been conducted at home and abroad.

The present invention is to provide a skin cosmetic excellent in safety and excellent in the prevention and improvement effect of various skin damage by taking advantage of the above-mentioned problems of the prior art. Natural situation mushroom extract of the present invention for achieving the above object has a situation mushroom (P. pini and P. linteus) that has been grown in the natural state of the North Korean peninsula, hot water extract, 20-30% ethanol extract, 1 It is an extract extracted with a mixture of, 3-butylene glycol, propylene glycol and water. In addition, the above-mentioned natural situation mushroom extract is applicable as a component of the skin cosmetic, and the content is preferably 0.05-30%.

In the present invention, the effect that can be used in cosmetics from the extract of hot water extracted from natural mushrooms grown in the natural state of North Korea, ethanol extract, butylene glycol extract, propylene glycol and a mixed solvent thereof, not artificially grown situation mushrooms It has been proved and excellent antioxidant effect, whitening effect, etc. when contained in cosmetics is to make the present invention. First, the whitening effect and antioxidant effect were investigated to confirm the availability of the skin extracts of situational mushroom extracts. For use as a sample, natural mushrooms were dried at room temperature until there was no change in weight, and then pulverized into a general mechanical powder. 20 g of this powder was added with 80 g of 30% aqueous propylene glycol solution and extracted by boiling in water at 60 ° C for 48 hours. The solution was filtered with a 0.45 µm filter paper and 80 g of 30% ethanol aqueous solution using 20 g of ethanol for cosmetics. After sterile filtration to 0.45㎛ (㎛), 20g of the situation mushroom was added to 80g of distilled water and treated with water bath at 80 ℃ for 48 hours, and then the mushroom and filtrate were separated with a mesh mesh of 400 mesh size and then continued. The sample was filtered through a 45.5-micron sterile filter. As a result of UV absorption spectrum analysis, the situation mushroom extracts had strong UV absorption ability. In addition, in the antioxidant test using linoleic acid as an effect test for application to skin cosmetics, ethanol extract of the situation mushroom was superior to the artificial mushroom cultivated mushroom extract (45%) and vitamin I (42.3%), which were antioxidant controls. It was found to have antioxidant effect (58%).

The effect of inhibiting tyrosinase activity, inhibiting melanogenesis of microorganisms (Streptomyces bikiniensis), and inhibiting melanogenesis of non-16-F1 melanoma cells were investigated. As a result, the concentration (IC ₅₀ ) that inhibits tyrosinase activity by 50% was less than 0.3%, and the melanin-producing region of the microorganism was inhibited by 25 mm at the concentration of 0,005%. In addition, the minimum concentration of inhibiting melanin production of B16-F1 melanoma cells was 0.05%, showing an excellent effect as a skin external preparation, especially a cosmetic. There is currently no example of application as a skin cosmetic containing the natural situation mushroom extract of the present invention. Although the amount of this natural mushroom extract to skin cosmetics is not limited, it is found that 0.05 to 30% by weight is appropriate, and the effect is not expected at too small content, and the effect is greatly increased even if it is mixed at too much content. Did not do it. In addition, the extract of the present invention is easily mixed with water and difficult to blend with fats and oils and organic solvents, but can be blended into the skin cosmetic even in a suspended state and does not have any influence on the effect. Skin external preparation of the present invention is suitable to be used as a skin cosmetic, and common ingredients such as oil, water, surfactants, moisturizers, lower alcohols, thickeners, chelating agents, pigments, preservatives, fragrances, etc. It is possible to combine them as necessary. Also in the present invention, the skin cosmetics are widely known for use on the skin, for example, the application of cosmetics such as lotion, latex, creams, packs and especially whitening cosmetics.

Hereinafter, the present invention will be described in more detail with reference to Examples.

These examples are merely illustrative of the present invention and the scope of the present invention is not limited to these examples.

Example 1

Situation mushrooms were imported P. pini and P. linteus native to North Korea on the Korean Peninsula. Situation mushrooms were dried at room temperature until there was no change in weight for sample use. After that, it was pulverized by a general mechanical method, and the two kinds of mushrooms were used in equal amounts. A variety of solvents were selected for solvent extraction, based on distilled water, ethanol, 1,3-butylene glycol, propylene glycol and combinations thereof. Extraction by water was added to the situation mushroom powder in water to 20% in a water bath at 60 ℃ 48 hours and then filtered through a mesh mesh of 400 mesh to isolate the mushroom and the filtered liquid. Subsequently, the separated filtrate was sterilized by 0.45 μm filter paper and used as a sample. The extraction by ethanol was carried out by leaving the ethane for water in a 30% solution for 15 days at room temperature with 20% of situation mushrooms, followed by a mesh of 400 mesh. The filtrate was separated from the mushroom and the filtrate. The separated filtrate was filtered with 0.45 μm filter paper to prepare a sample, and the same effect test as in the following example was performed. In the extraction by the mixture of 1,3-butylene, propylene glycol and water, the ratio of 1,3-butylene, propylene glycol and water is mixed in a weight ratio of 8 to 2 and 4 to 6, and 20% by weight of the situation mushroom The mixture was heated at 60 ° C. for 48 hours, filtered through a mesh mesh of 400 mesh, mushrooms and filtrates were separated, and the filtrate was filtered with 0.45 μm filter paper to prepare a sample.

Example 2

In this example, the degree of inhibition of this automatic oxidation was compared using linoleic acid, which is a well-oxidized material. In an 8 ml captube, 3 ml of 10 mM linoleic acid solution and 0.075 ml of sample adjusted to 4 mg / ml were prepared. After addition, it is left at 4 ° C. 0.1 ml of the mixed solution was added to a test tube containing 4.7 ml of 75% ethanol aqueous solution after 24 hours, followed by stirring. Then, 0.1 ml of 30% ammonium thiocyanate solution and 0.1 ml of ferrous chloride reagent were added thereto for 3 minutes. After leaving, color was absorbed and the absorption wavelength was measured at 500 nm. The antioxidant effect of the substance was measured in% compared to the control group without the sample. In the antioxidant effect test using linoleic acid automatic oxidation, the natural situation mushroom extract extracted in Example 1 was superior to the control group and showed superior antioxidant effect (58%) compared to vitamin E (42.3%). One).

Table 1

Example 3

This experiment was to determine the whitening effect by looking at the degree of functional inhibition of the enzyme tyrosinase to determine the whitening effect of the situation mushroom extract extracted in Example 1. Tyrosinase is an enzyme that helps melanin production by promoting the oxidation of a substance called tyrosine in vivo. In this embodiment, the method of inhibiting the function of this enzyme (Sigma (USA)), to inhibit the tyrosine oxidized to form a black polymer called melanin (Pomerantz SH, The tyrosine hydroxylase activity of mammalian tyrosinase, J) Biochem., 24, 16l-l68 (1966)) was applied as a whitening effect. Morus extract (volume / volume% for distilled water of stock solution, 10, 1, 0.1, 0.01), natural acid, artificial acid situation mushroom extract (volume / volume for distilled water of stock solution, 0.5, 0.05, 0.005, 00005) Inhibition of tyrosinase activity was investigated by using a sample of Gojic Acid, Arbutin, Hydroquinone, and P-methoxyphenol (volume / volume% of distilled water of 1, 0.1, 0.01, 0.001, 0.0001) as a sample.

For inhibitory activity against tyrosinase, 15 μl of the sample was placed in a microplate (96 wells), 150 μl of 0.1 mol phosphate buffer (pH6.86) and 25 μl of 1.5 mM L-tyrosine solution were added, followed by 2,380 unit / ml mushroom tyrosinase (0.05). mol phosphate buffer, pH6.86) 7 μl was added and reacted at 30 ° C. for 10 minutes, and measured at 490 nm using a microplate. Inhibition rate (%) for tyrosinase was calculated by the following equation, IC ₅₀ value was determined by the concentration of inhibitors reaching 50% enzyme inhibition rate (Table 2).

% Inhibition = {[(D-C)-(B-A)] / (D-C)} × 100

A: absorbance before reaction of the inhibitor

B: absorbance after the reaction of the inhibitor

C: Absorbance before reaction of without inhibitor

D: absorbance after the reaction without the inhibitor

As a result of tyrosinase activity inhibition test, the IC ₅₀ value of each extract of wild situation mushroom was less than 0.2%. This effect was higher than artificial cultivated situation mushrooms and had better effects than arbutin, which is known to have excellent whitening effect.

Table 2

Example 4

This experiment was used to measure the melanin inhibitory activity against actinomycetes (Streptomyces bikiniensis NRRL B-1049) was used from KCTC (Korean Collection for Type Culture, Accession No. 9172).

Incubate Streptomyces bikiniensis NRRL B-1049 at 28 ° C for 2 weeks in papavizas ′ VDYA agar slant medium (V-8 juice 2O0ml, glucose 2g, yeast extract 2g, CaCO ₃ 1g, agar 20g, distilled water 800ml, pH7.2) Spores were produced to produce spores with sterile water. 0.2 ml each of the spore suspension was applied to ISP No. 7 plate medium to which 0.2% yeast extract was added, and then the amount of the sample was applied to the paper disc soaked on the surface of the medium with 30 ㎍ / paper disc and incubated at 28 ° C. The inhibition of melanogenesis was observed using the 4-hydroxyanisole known as the melanogenesis inhibitor as a control for the size of the melanogenesis inhibitors produced after 48 hours of incubation (Table 3).

The inhibitory activity of melanin-producing activity against Streptomyces bikiniensis NRRL B-1049 of wild-state extracts showed 30 mm melanin-inhibiting activity in natural ethanol extracts. This inhibitory activity was higher than that of 4-hydroxyanisole, a potent melanin-inhibiting agent Excellent activity was shown.

Table 3

Example 4

This experiment was observed at the cellular level to investigate the skin whitening effect of the extract extracted with hot water extract, ethanol extract, 1,3-butylene glycol, propylene glycol and water mixture of citric acid situation mushroom.

The B16-Fl melanoma cells used in this experiment are cell strains derived from mice and are cells that distribute a melanin called melanin. The cells were treated with material during artificial culture and the degree of melanin black pigment reduction was compared.

B16-F1 melanoma cells used in this experiment were used from the American Type Culture Collection, Accession No .: 6323 (ATCC).

The inhibition of melanin biosynthesis of B16 melanoma cells was determined as follows. B16 melanoma cells were suspended in Dulbecco's Modified Eagle's Medium (Gibco, USA) containing 10% fetal bovine serum at a concentration of 5 × 10 ³ cels / ml. Suspension cells (5 ml) were placed in a tissue culture flask, and 0.134% of the assay sample was added, followed by incubation at 37 ° C under 5% carbon dioxide-95% air. After incubation for 4 days, the melanogenesis level was first determined by the color of the culture medium, and the cells were washed with phosphate buffer and trypsinized to separate the cells from the flask. The cells were collected in a tube, washed with phosphate buffer, and centrifuged to compare melanin secretion levels between several samples known as whitening agents and situation mushroom extracts. The evaluation method was evaluated by visual observation of the degree of color change according to the treatment of nothing in the cell culture and the degree of black melanin pigment secretion when each sample was treated. As a result, natural situation mushroom extract effectively inhibited melanin pigment secretion (Table 4).

Table 4.

Examples 5,6,7

In this example, a comparative experiment of the cosmetics containing the natural situation mushroom extract extracted in Example 1 was conducted. The composition of the cream used in the comparative experiment is shown in Table 5. First, the phase b) described in Table 5 is heated and stored at 70 ° C. To this, a) phase is added, and after pre-emulsification, it is emulsified uniformly with a homomixer, and it cools gradually, and prepares a cream (Example 5-7, a comparative example). Each cream of Table 5 was applied to the right side of five subjects (20-35 year old female) twice a day for 1 consecutive month. After completion of the experiment, the skins of the applied areas on the left and right sides of the face were compared by using an image analyzer, and the darkest color was set as 5 medium color and 3 as the brightest color. Table 6 compares the facial skin color of the experimenter using the cream made in Example 5 with the facial use area of the experimenter using the cream made of the comparative example. As shown in Table 6, it can be seen that the whitening effect appeared on the face of the experimenter who applied the cream containing the situation extract.

Table 5

i

Table 6

Other examples are shown below. That is, as shown above, it showed an excellent effect on skin improvement and a skin beauty effect.

Example 8

To 8 g of 95% ethanol, 0.05 g of polypyrrolidone, 0.1 g of oleyl alcohol, 0.2 g of polyoxyethylene monooleate, 0.2 g of fragrance, 0.1 g of paraoxybenzoic acid meryl ester, a small amount of antioxidant, and a small amount of pigment are dissolved. . This was added with stirring in a solution of 0.05 g of natural situation mushroom extract and 5 g of glycerin in 85.33 g of purified water to obtain a lotion having a skin-improving effect.

Example 9

1.2 g of cetyl alcohol, 10 g of squalane, 2 g of vaseline, 0.2 g of paraoxybenzoic acid ethyl ester, 1 g of glycerin monoesteralide, 1 g of polyoxyethylene (20 mol added) monooleate and 0.1 g of fragrance at 70 ° C. 0.5 g of a situation mushroom extract, 5 g of dipropylene glycol, 2 g of polyethylene glycol 1500, 0.2 g of triethanolamine, and 76.2 g of purified water are heated to 75 ° C. to dissolve it by heating. Both are emulsified by mixing, followed by cooling to obtain an emulsion having a water / oil-based skin improvement effect.

Example 10

5 g of 95% ethyl alcohol, 1.2 g of polyoxyethylene sorbitan monooleate, 0.3 g of seaweed extract, 0.2 g of sodium hyaluronate, 0.2 g of vitamin acetate, 0.2 g of sodium licorice, 0.1 g of paraoxybenzoate, 0.1 g of natural products 1g of mushroom extract and a suitable amount of pigment are mixed to obtain a cosmetic liquid having an effect of skin improvement.

Natural situation mushroom extract according to the present invention is excellent in various skin improvement effect and very excellent in terms of safety. In addition, the above-mentioned natural situation mushroom extract can be applied to skin cosmetics and has excellent whitening and antioxidant effects when contained in cosmetics.

Claims (6)

Characterized by skin cosmetics containing natural P. pini and P. linteus extracts The dried and pulverized natural situation mushroom is extracted with a mixed solution of 1,3-butylene glycol, propylene glycol and distilled water. The dried situation mushroom, characterized in that extracted with 30% to 95% ethanol aqueous solution. It is characterized by the extraction of hot natural dry mushrooms with distilled water. The mixing ratio of 1,3-butylene glycol, propylene glycol and distilled water in the mixed solution of 1,3-butylene glycol, propylene glycol and distilled water of claim 2 is characterized in that the temperature at the time of extraction After sterilizing filtration after heating to room temperature to 60 ℃. Skin cosmetics, characterized in that it contains 0.05 to 30% of the natural situation mushroom extract of claim 1