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IN2012DN02396A - - Google Patents

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Publication number
IN2012DN02396A
IN2012DN02396A IN2396DEN2012A IN2012DN02396A IN 2012DN02396 A IN2012DN02396 A IN 2012DN02396A IN 2396DEN2012 A IN2396DEN2012 A IN 2396DEN2012A IN 2012DN02396 A IN2012DN02396 A IN 2012DN02396A
Authority
IN
India
Prior art keywords
isobutanol
cells
isolation
observed
developed
Prior art date
Application number
Inventor
Brian James Paul
Wonchul Suh
Original Assignee
Butamax Tm Advanced Biofuels
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Butamax Tm Advanced Biofuels filed Critical Butamax Tm Advanced Biofuels
Publication of IN2012DN02396A publication Critical patent/IN2012DN02396A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/16—Butanols
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/746—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for lactic acid bacteria (Streptococcus; Lactococcus; Lactobacillus; Pediococcus; Enterococcus; Leuconostoc; Propionibacterium; Bifidobacterium; Sporolactobacillus)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01027—L-Lactate dehydrogenase (1.1.1.27)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01054—Formate C-acetyltransferase (2.3.1.54), i.e. pyruvate formate-lyase or PFL
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/01005—Acetolactate decarboxylase (4.1.1.5)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

An engineering method was developed to allow genetic modification and isolation of lactic acid bacteria cells that lack lactate dehydrogenase.and acetolactate decarboxylase activities. In cells with these modifications and an isobutanol biosynthetic pathway, improved production of isobutanol was observed.
IN2396DEN2012 2009-09-29 2010-09-29 IN2012DN02396A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US24671709P 2009-09-29 2009-09-29
PCT/US2010/050705 WO2011041402A1 (en) 2009-09-29 2010-09-29 Improved flux to acetolactate-derived products in lactic acid bacteria

Publications (1)

Publication Number Publication Date
IN2012DN02396A true IN2012DN02396A (en) 2015-08-21

Family

ID=43093547

Family Applications (1)

Application Number Title Priority Date Filing Date
IN2396DEN2012 IN2012DN02396A (en) 2009-09-29 2010-09-29

Country Status (9)

Country Link
US (1) US9169499B2 (en)
EP (1) EP2483400A1 (en)
JP (1) JP2013505739A (en)
CN (1) CN102712912A (en)
AU (1) AU2010300722A1 (en)
BR (1) BR112012006941A2 (en)
CA (1) CA2776151A1 (en)
IN (1) IN2012DN02396A (en)
WO (1) WO2011041402A1 (en)

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US8188250B2 (en) 2008-04-28 2012-05-29 Butamax(Tm) Advanced Biofuels Llc Butanol dehydrogenase enzyme from the bacterium Achromobacter xylosoxidans
MX2011003313A (en) 2008-09-29 2011-06-16 Butamax Tm Advanced Biofuels INCREASED HETEROLOGOUS Fe-S ENZYME ACTIIVTY IN YEAST.
WO2011041426A1 (en) * 2009-09-29 2011-04-07 Butamax(Tm) Advanced Biofuels Llc Improved yeast production host cells
NZ600509A (en) 2009-12-29 2014-08-29 Butamax Tm Advanced Biofuels Alcohol dehydrogenases (adh) useful for fermentive production of lower alkyl alcohols
CA2785660A1 (en) 2009-12-29 2011-07-07 Butamax(Tm) Advanced Biofuels Llc Expression of hexose kinase in recombinant host cells
MX348605B (en) 2010-02-17 2017-06-21 Butamax(Tm) Advanced Biofuels Llc Improving activity of fe-s cluster requiring proteins.
CA2810244C (en) 2010-09-07 2020-10-06 Butamax(Tm) Advanced Biofuels Llc Integration of a polynucleotide encoding a polypeptide that catalyzes pyruvate to acetolactate conversion
WO2012129555A2 (en) 2011-03-24 2012-09-27 Butamax (Tm) Advanced Biofuels Llc Host cells and methods for production of isobutanol
AU2012249389A1 (en) 2011-04-29 2013-11-14 Danisco Us Inc. Production of mevalonate, isoprene, and isoprenoids using genes encoding polypeptides having thiolase, HMG-CoA synthase and HMG-CoA reductase enzymatic activities
EP2737073B1 (en) 2011-07-28 2021-10-20 Butamax Advanced Biofuels LLC Keto-isovalerate decarboxylase enzymes and methods of use thereof
CA2861613A1 (en) 2011-12-30 2013-07-04 Butamax Advanced Biofuels Llc Fermentative production of alcohols
CN102559736B (en) * 2012-02-24 2014-01-08 常茂生物化学工程股份有限公司 Gene engineering bacterial strain capable of improving yield of fumaric acid and establishing method and application thereof
JP2015510774A (en) 2012-03-23 2015-04-13 ビュータマックス・アドバンスド・バイオフューエルズ・エルエルシー Acetic acid supplementation of medium for butanol-producing bacteria
EP2847326A2 (en) 2012-05-11 2015-03-18 Butamax Advanced Biofuels LLC Ketol-acid reductoisomerase enzymes and methods of use
CN102816787B (en) * 2012-06-20 2015-05-06 江南大学 Highly expressed Staphyloccocus aureus alpha-acetolacetate decearboxylase by utilization of recombinant escherichia coli
WO2014025604A2 (en) * 2012-08-07 2014-02-13 Gevo, Inc. Microorganisms for improved production of fuels, chemicals, and amino acids
EP2888350A1 (en) 2012-08-22 2015-07-01 Butamax Advanced Biofuels LLC Production of fermentation products
CN102876704A (en) * 2012-09-20 2013-01-16 江南大学 Method for expressing staphyloccocus aureus alpha-acetolactate decarboxylase by utilizing recombinant bacillus subtilis efficiently
US9840724B2 (en) 2012-09-21 2017-12-12 Butamax Advanced Biofuels Llc Production of renewable hydrocarbon compositions
JP6407869B2 (en) 2012-09-26 2018-10-17 ビュータマックス・アドバンスド・バイオフューエルズ・エルエルシー Polypeptide having ketol acid reductoisomerase activity
US9273330B2 (en) 2012-10-03 2016-03-01 Butamax Advanced Biofuels Llc Butanol tolerance in microorganisms
WO2014106107A2 (en) 2012-12-28 2014-07-03 Butamax (Tm) Advanced Biofuels Llc Dhad variants for butanol production
ES2735024T3 (en) 2013-03-14 2019-12-13 Du Pont Glycerol-3-phosphate dehydrogenase for butanol production
US9771602B2 (en) 2013-03-15 2017-09-26 Butamax Advanced Biofuels Llc Competitive growth and/or production advantage for butanologen microorganism
US9580705B2 (en) 2013-03-15 2017-02-28 Butamax Advanced Biofuels Llc DHAD variants and methods of screening
US20150122558A1 (en) 2013-09-09 2015-05-07 Columbia Trailer Co., Inc. Lifting jack assembly with rotatable hydraulic cylinder for steering and method of operation
EP3072955B1 (en) * 2013-11-22 2019-01-09 JMTC Enzyme Corporation Transformant and process for production thereof, and process for production of lactic acid
EP3180440A4 (en) 2014-08-11 2018-01-10 Butamax Advanced Biofuels LLC Yeast preparations and methods of making the same
EP3309247A4 (en) * 2015-06-12 2019-01-09 Cj Cheiljedang Corporation Microorganism having improved production of lactic acid and method for producing lactic acid by using same
WO2017060455A1 (en) * 2015-10-09 2017-04-13 Danmarks Tekniske Universitet High-level production of diacetyl in a metabolically engineered lactic acid bacterium
CN110312792B (en) 2016-12-14 2024-04-09 瓦赫宁根大学 Thermostable Cas9 nuclease
CN108570438B (en) * 2017-03-08 2020-06-30 中国科学院上海高等研究院 Method and modified bacteria for enhancing the production of α-ketoisovalerate by Klebsiella pneumoniae
CN108486176B (en) * 2018-05-18 2021-08-03 天津科技大学 A kind of Saccharomyces cerevisiae producing ethyl lactate and its construction method and application
IT201800007846A1 (en) * 2018-08-03 2020-02-03 Università Degli Studi Di Milano - Bicocca PROCEDURE FOR THE CELLULAR BIOSYNTHESIS OF POLY-LACTIC ACID AND POLY-LACTIC ACID
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Also Published As

Publication number Publication date
JP2013505739A (en) 2013-02-21
AU2010300722A1 (en) 2012-04-12
EP2483400A1 (en) 2012-08-08
US20110136192A1 (en) 2011-06-09
WO2011041402A1 (en) 2011-04-07
CA2776151A1 (en) 2011-04-07
CN102712912A (en) 2012-10-03
BR112012006941A2 (en) 2015-09-08
US9169499B2 (en) 2015-10-27

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