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HK1006857A1 - Multiply mutated subtilisins - Google Patents

Multiply mutated subtilisins Download PDF

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Publication number
HK1006857A1
HK1006857A1 HK98106058A HK98106058A HK1006857A1 HK 1006857 A1 HK1006857 A1 HK 1006857A1 HK 98106058 A HK98106058 A HK 98106058A HK 98106058 A HK98106058 A HK 98106058A HK 1006857 A1 HK1006857 A1 HK 1006857A1
Authority
HK
Hong Kong
Prior art keywords
subtilisin
ser
dna
asp
subtilis
Prior art date
Application number
HK98106058A
Other languages
German (de)
English (en)
Chinese (zh)
Other versions
HK1006857B (en
Inventor
M. Zukowski Mark
O. Narhi Linda
Levitt Michael
Original Assignee
Amgen Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amgen Inc. filed Critical Amgen Inc.
Publication of HK1006857B publication Critical patent/HK1006857B/en
Publication of HK1006857A1 publication Critical patent/HK1006857A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Detergent Compositions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Cosmetics (AREA)
  • Tires In General (AREA)

Claims (4)

  1. Analogue de subtilisine [Asp76, Ser109, Ser218, Ala222] sensiblement pur, éventuellement avec une substitution supplémentaire choisie à partir de [Leu31] et [Leu124].
  2. Composition comprenant une quantité efficace d'un analogue de subtilisine de la revendication 1 dans une formulation détergente.
  3. Procédé pour la production d'analogues de subtilisine, comprenant la production, de façon recombinante, d'un analogue de subtilisine à partir d'une cellule hôte ayant un acide nucléique codant un analogue de subtilisine [Asp76, Ser109, Ser218, Ala222] éventuellement avec une substitution supplémentaire choisie à partir de [Leu31] et [Leu124], et la purification de l'analogue de subtilisine à partir du bouillon de fermentation pour obtenir un analogue de subtilisine [Asp76, Ser109, Ser218, Ala222] sensiblement pur, éventuellement avec une substitution supplémentaire choisie à partir de [Leu31] et [Leu124l
  4. Utilisation d'une quantité efficace d'un analogue de subtilisine [Asp76, Ser109, Ser218, Ala222] sensiblement pur, éventuellement avec une substitution supplémentaire choisie à partir de [Leu31] et [Leu124] dans la fabrication d'une composition détergente.
HK98106058A 1989-05-17 1998-06-23 Multiply mutated subtilisins HK1006857A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US35312489A 1989-05-17 1989-05-17
US353124 1989-05-17

Publications (2)

Publication Number Publication Date
HK1006857B HK1006857B (en) 1999-03-19
HK1006857A1 true HK1006857A1 (en) 1999-03-19

Family

ID=23387862

Family Applications (1)

Application Number Title Priority Date Filing Date
HK98106058A HK1006857A1 (en) 1989-05-17 1998-06-23 Multiply mutated subtilisins

Country Status (14)

Country Link
US (1) US5397705A (fr)
EP (1) EP0398539B1 (fr)
JP (1) JPH03505976A (fr)
KR (1) KR100188532B1 (fr)
AT (1) ATE141324T1 (fr)
AU (1) AU618675B2 (fr)
CA (1) CA2016211C (fr)
DE (1) DE69028040T2 (fr)
DK (1) DK0398539T3 (fr)
ES (1) ES2091224T3 (fr)
GR (1) GR3021010T3 (fr)
HK (1) HK1006857A1 (fr)
SG (1) SG47797A1 (fr)
WO (1) WO1990014420A1 (fr)

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US5665587A (en) * 1989-06-26 1997-09-09 Novo Nordisk A/S Modified subtilisins and detergent compositions containing same
DK271490D0 (da) * 1990-11-14 1990-11-14 Novo Nordisk As Detergentkomposition
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SK43094A3 (en) * 1991-10-16 1994-09-07 Unilever Nv Aqueous enzymatic detergent compositions
US5989887A (en) * 1992-11-25 1999-11-23 Dsm N.V. Cloning and expression of DNA molecules incoding arabinan-degrading enzymes of fungal origin
US5567601A (en) * 1993-06-01 1996-10-22 University Of Maryland Subtilisin mutants lacking a primary calcium binding site
US6436690B1 (en) 1993-09-15 2002-08-20 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted
US6440717B1 (en) 1993-09-15 2002-08-27 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis
ATE361355T1 (de) * 1993-10-14 2007-05-15 Procter & Gamble Proteasehaltige reinigungsmittel
MA23346A1 (fr) * 1993-10-14 1995-04-01 Genencor Int Variantes de la subtilisine
CN1177921C (zh) * 1993-10-14 2004-12-01 普罗格特-甘布尔公司 含有蛋白酶的漂白组合物
DE4411223A1 (de) * 1994-03-31 1995-10-05 Solvay Enzymes Gmbh & Co Kg Verwendung alkalischer Proteasen in gewerblichen Textilwaschverfahren
US6599730B1 (en) 1994-05-02 2003-07-29 Procter & Gamble Company Subtilisin 309 variants having decreased adsorption and increased hydrolysis
US6066611A (en) * 1994-10-13 2000-05-23 The Procter & Gamble Company Bleaching compositions comprising protease enzymes
US6455295B1 (en) 1995-03-08 2002-09-24 The Procter & Gamble Company Subtilisin Carlsberg variants having decreased adsorption and increased hydrolysis
IL117350A0 (en) 1995-03-09 1996-07-23 Procter & Gamble Proteinase k variants having decreased adsorption and increased hydrolysis
US6475765B1 (en) 1995-03-09 2002-11-05 Procter & Gamble Company Subtilisin DY variants having decreased adsorption and increased hydrolysis
TR199901358T2 (xx) * 1996-12-20 1999-10-21 Unilever N.V. Enzimli a�artma bile�imi.
US6204234B1 (en) * 1997-07-09 2001-03-20 The Proctor & Gamble Company Cleaning compositions comprising a specific oxygenase
ES2368718T3 (es) * 1997-10-23 2011-11-21 Danisco Us Inc. Variantes de subtilisina con múltiples sustituciones.
MA24811A1 (fr) * 1997-10-23 1999-12-31 Procter & Gamble Compositions de lavage contenant des variantes de proteases multisubstituees
KR20010034697A (ko) * 1998-03-26 2001-04-25 데이비드 엠 모이어 아미노산 치환을 갖는 세린 프로테아제 변이체
US6908757B1 (en) 1998-03-26 2005-06-21 The Procter & Gamble Company Serine protease variants having amino acid deletions and substitutions
MXPA02000842A (es) 1999-07-22 2002-07-30 Procter & Gamble Conjugados de proteasa que tienen sitios de corte protegidos estericamente.
US6946128B1 (en) 1999-07-22 2005-09-20 The Procter & Gamble Company Protease conjugates having sterically protected epitope regions
BR0012660A (pt) 1999-07-22 2002-04-09 Procter & Gamble Variante de protease tipo subtilisina; composição de limpeza; e composição de cuidado pessoal
CN1373802A (zh) 1999-07-22 2002-10-09 宝洁公司 在特定表位区域具有氨基酸缺失和取代的枯草杆菌蛋白酶变体
AU6820000A (en) 1999-08-31 2001-03-26 Novozymes A/S Novel proteases and variants thereof
US7217554B2 (en) 1999-08-31 2007-05-15 Novozymes A/S Proteases and variants thereof
US6727085B2 (en) * 1999-12-15 2004-04-27 Fanoe Tina Sejersgaard Subtilase variants having an improved wash performance on egg stains
AU2001280968A1 (en) * 2000-07-31 2002-02-13 Menzel, Rolf Compositions and methods for directed gene assembly
EP1998266A3 (fr) 2001-02-19 2009-02-11 Merck Patent GmbH Procédé d'identification d'épitopes de cellules t et utilisation pour préparer des molécules avec immunogénicité réduite
PL210859B1 (pl) * 2001-03-23 2012-03-30 Genencor Int Odmiana subtylizyny zawierająca zmieniony epitop limfocytów T, kwas nukleinowy kodujący tę odmianę subtylizyny, wektor ekspresyjny i komórka gospodarza zawierająca kwas nukleinowy oraz kompozycje zawierające tę odmianę subtylizyny
AU2002367465A1 (en) * 2001-07-09 2003-09-29 Children's Hospital Medical Center Of Akron Multiple controls for molecular genetic analyses
DE10153792A1 (de) 2001-10-31 2003-05-22 Henkel Kgaa Neue Alkalische Protease-Varianten und Wasch- und Reinigungsmittel enthaltend diese neuen Alkalischen Protease-Varianten
CA2472723C (fr) * 2002-01-16 2013-12-17 Genencor International, Inc. Variants de protease a substitutions multiples
US7888093B2 (en) * 2002-11-06 2011-02-15 Novozymes A/S Subtilase variants
US7294499B2 (en) 2003-01-30 2007-11-13 Novozymes A/S Subtilases
US7727756B2 (en) * 2003-03-21 2010-06-01 Novozymes A/S Subtilases
EP1623029B1 (fr) * 2003-05-07 2008-09-03 Novozymes A/S Enzymes de subtilisine variant (subtilases)
WO2020112599A1 (fr) * 2018-11-28 2020-06-04 Danisco Us Inc Variants de subtilisine à stabilité améliorée
PE20251535A1 (es) 2022-05-14 2025-06-05 Novozymes As Composiciones y metodos para prevenir, tratar, suprimir y/o eliminar infecciones e infestaciones fitopatogenas

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4760025A (en) * 1984-05-29 1988-07-26 Genencor, Inc. Modified enzymes and methods for making same
US5155033A (en) * 1989-01-06 1992-10-13 Genencor, Inc. Subtilisins modified at position 225 resulting in a shift in catalytic activity
EP0254735B2 (fr) * 1986-01-15 1998-06-17 Amgen Inc. PROCEDE DE PRODUCTION D'ANALOGUES DE SUBTILISINE THERMIQUEMENT STABLE ET STABLE AU pH
US5013657A (en) * 1988-04-12 1991-05-07 Bryan Philip N Subtilisin mutations
US4980288A (en) * 1986-02-12 1990-12-25 Genex Corporation Subtilisin with increased thermal stability
US4990452A (en) * 1986-02-12 1991-02-05 Genex Corporation Combining mutations for stabilization of subtilisin
DE3750916T2 (de) * 1986-04-30 1995-06-01 Genencor Int Mutante einer nicht menschlichen Carbonyl-Hydrolase, für diese kodierende DNS-Sequenzen und Vektoren und durch diese Vektoren transformierte Wirte.
NZ221627A (en) * 1986-09-09 1993-04-28 Genencor Inc Preparation of enzymes, modifications, catalytic triads to alter ratios or transesterification/hydrolysis ratios
ES2134186T3 (es) * 1987-02-27 1999-10-01 Genencor Int Clonaje molecular y expresion de genes que codifican enzimas proteoliticas.
EP0916732B1 (fr) * 1987-04-06 2007-08-22 Novozymes A/S Stabilisation de protéines par mise au point des interactions électrostatiques aux sites de liaison d'ions métalliques
US4914031A (en) * 1987-04-10 1990-04-03 Amgen, Inc. Subtilisin analogs
FR2614467A1 (fr) * 1987-04-21 1988-10-28 Telemecanique Electrique Appareil interrupteur de protection a mecanisme de declenchement simplifie

Also Published As

Publication number Publication date
ATE141324T1 (de) 1996-08-15
CA2016211A1 (fr) 1990-11-17
EP0398539A1 (fr) 1990-11-22
SG47797A1 (en) 1998-04-17
ES2091224T3 (es) 1996-11-01
AU5558090A (en) 1990-12-18
GR3021010T3 (en) 1996-12-31
CA2016211C (fr) 1998-07-21
DE69028040T2 (de) 1997-02-27
DK0398539T3 (da) 1996-12-09
DE69028040D1 (de) 1996-09-19
US5397705A (en) 1995-03-14
EP0398539B1 (fr) 1996-08-14
JPH03505976A (ja) 1991-12-26
WO1990014420A1 (fr) 1990-11-29
KR920701426A (ko) 1992-08-11
AU618675B2 (en) 1992-01-02
KR100188532B1 (ko) 1999-06-01

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