[go: up one dir, main page]

ES2091224T3 - Subtilisinas con mutaciones multiples. - Google Patents

Subtilisinas con mutaciones multiples.

Info

Publication number
ES2091224T3
ES2091224T3 ES90304715T ES90304715T ES2091224T3 ES 2091224 T3 ES2091224 T3 ES 2091224T3 ES 90304715 T ES90304715 T ES 90304715T ES 90304715 T ES90304715 T ES 90304715T ES 2091224 T3 ES2091224 T3 ES 2091224T3
Authority
ES
Spain
Prior art keywords
subtilisins
multiple mutations
subtilisin
admixture
expressing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90304715T
Other languages
English (en)
Inventor
Mark M Zukowski
Linda O Narhi
Michael Levitt
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Amgen Inc
Original Assignee
Amgen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amgen Inc filed Critical Amgen Inc
Application granted granted Critical
Publication of ES2091224T3 publication Critical patent/ES2091224T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Detergent Compositions (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Cosmetics (AREA)
  • Tires In General (AREA)

Abstract

UNA CLASE DE ANALOGOS DE LA SUBTILISINA ADECUADA PARA SER MEZCLADA CON COMPOSICIONES DE LIMPIEZA Y CON UNA ESTABILIDAD MEJORADA SOBRE EL BACILO DE LA SUBTISILINA. ESTOS ANALOGOS SON PREPARADOS EXPRESANDO UN GEN MODIFICADO CODIFICADOR DEL ANALOGO DE LA SUBTILISINA EN EL BACILLUS SUBTILIS.
ES90304715T 1989-05-17 1990-05-01 Subtilisinas con mutaciones multiples. Expired - Lifetime ES2091224T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US35312489A 1989-05-17 1989-05-17

Publications (1)

Publication Number Publication Date
ES2091224T3 true ES2091224T3 (es) 1996-11-01

Family

ID=23387862

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90304715T Expired - Lifetime ES2091224T3 (es) 1989-05-17 1990-05-01 Subtilisinas con mutaciones multiples.

Country Status (13)

Country Link
US (1) US5397705A (es)
EP (1) EP0398539B1 (es)
JP (1) JPH03505976A (es)
KR (1) KR100188532B1 (es)
AT (1) ATE141324T1 (es)
AU (1) AU618675B2 (es)
CA (1) CA2016211C (es)
DE (1) DE69028040T2 (es)
DK (1) DK0398539T3 (es)
ES (1) ES2091224T3 (es)
GR (1) GR3021010T3 (es)
SG (1) SG47797A1 (es)
WO (1) WO1990014420A1 (es)

Families Citing this family (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE184316T1 (de) * 1987-04-06 1999-09-15 Novo Nordisk As Regelung von elektrostatischen interaktionen an metallionen-bindungsstellen zur stabilisierung von proteinen
US5665587A (en) * 1989-06-26 1997-09-09 Novo Nordisk A/S Modified subtilisins and detergent compositions containing same
DK271490D0 (da) * 1990-11-14 1990-11-14 Novo Nordisk As Detergentkomposition
US5863783A (en) * 1991-03-27 1999-01-26 Gist-Brocades, N.V. Cloning and expression of DNA molecules encoding arabinan-degrading enzymes of fungal origin
CA2119362A1 (en) * 1991-10-16 1993-04-29 Carlo J. Van Den Bergh Aqueous enzymatic detergent compositions
US5989887A (en) * 1992-11-25 1999-11-23 Dsm N.V. Cloning and expression of DNA molecules incoding arabinan-degrading enzymes of fungal origin
US5567601A (en) * 1993-06-01 1996-10-22 University Of Maryland Subtilisin mutants lacking a primary calcium binding site
US6436690B1 (en) 1993-09-15 2002-08-20 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted
US6440717B1 (en) 1993-09-15 2002-08-27 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis
CZ105296A3 (en) * 1993-10-14 1996-09-11 Procter & Gamble Bleaching agent containing protease enzymes, cleansing agents, agent for cleaning fabrics and fabric cleaning method
MA23346A1 (fr) * 1993-10-14 1995-04-01 Genencor Int Variantes de la subtilisine
ES2287931T3 (es) * 1993-10-14 2007-12-16 THE PROCTER & GAMBLE COMPANY Composiciones limpiadoras que contienen proteasa.
DE4411223A1 (de) * 1994-03-31 1995-10-05 Solvay Enzymes Gmbh & Co Kg Verwendung alkalischer Proteasen in gewerblichen Textilwaschverfahren
US6599730B1 (en) 1994-05-02 2003-07-29 Procter & Gamble Company Subtilisin 309 variants having decreased adsorption and increased hydrolysis
US6066611A (en) * 1994-10-13 2000-05-23 The Procter & Gamble Company Bleaching compositions comprising protease enzymes
US6455295B1 (en) 1995-03-08 2002-09-24 The Procter & Gamble Company Subtilisin Carlsberg variants having decreased adsorption and increased hydrolysis
US6475765B1 (en) 1995-03-09 2002-11-05 Procter & Gamble Company Subtilisin DY variants having decreased adsorption and increased hydrolysis
IL117350A0 (en) 1995-03-09 1996-07-23 Procter & Gamble Proteinase k variants having decreased adsorption and increased hydrolysis
WO1998028400A2 (en) * 1996-12-20 1998-07-02 Unilever N.V. Enzymatic bleach composition
US6204234B1 (en) * 1997-07-09 2001-03-20 The Proctor & Gamble Company Cleaning compositions comprising a specific oxygenase
ES2368718T3 (es) * 1997-10-23 2011-11-21 Danisco Us Inc. Variantes de subtilisina con múltiples sustituciones.
AR015977A1 (es) * 1997-10-23 2001-05-30 Genencor Int Variantes de proteasa multiplemente substituida con carga neta alterada para su empleo en detergentes
US6908757B1 (en) 1998-03-26 2005-06-21 The Procter & Gamble Company Serine protease variants having amino acid deletions and substitutions
US6569663B1 (en) 1998-03-26 2003-05-27 The Procter & Gamble Company Serine protease variants having amino acid substitutions
JP2003505070A (ja) 1999-07-22 2003-02-12 ザ、プロクター、エンド、ギャンブル、カンパニー 定められたエピトープ領域においてアミノ酸置換を有するサブチリシンプロテアーゼ変異体
US6946128B1 (en) 1999-07-22 2005-09-20 The Procter & Gamble Company Protease conjugates having sterically protected epitope regions
CA2379712A1 (en) 1999-07-22 2001-02-01 The Procter & Gamble Company Subtilisin protease variants having amino acid deletions and substitutions in defined epitope regions
CN1377406A (zh) 1999-07-22 2002-10-30 宝洁公司 具有空间保护的切割位点的蛋白酶缀合物
AU6820000A (en) * 1999-08-31 2001-03-26 Novozymes A/S Novel proteases and variants thereof
US7217554B2 (en) 1999-08-31 2007-05-15 Novozymes A/S Proteases and variants thereof
US6727085B2 (en) * 1999-12-15 2004-04-27 Fanoe Tina Sejersgaard Subtilase variants having an improved wash performance on egg stains
US7087415B2 (en) * 2000-07-31 2006-08-08 Athena Biotechnologies, Inc. Methods and compositions for directed gene assembly
EP1998266A3 (en) 2001-02-19 2009-02-11 Merck Patent GmbH Method for identification of T-cell epitopes and use for preparing molecules with reduced immunogenicity
PL210859B1 (pl) * 2001-03-23 2012-03-30 Genencor Int Odmiana subtylizyny zawierająca zmieniony epitop limfocytów T, kwas nukleinowy kodujący tę odmianę subtylizyny, wektor ekspresyjny i komórka gospodarza zawierająca kwas nukleinowy oraz kompozycje zawierające tę odmianę subtylizyny
US20050227229A1 (en) * 2001-07-09 2005-10-13 Lebo Roger V Multiple controls for molecular genetic analyses
DE10153792A1 (de) 2001-10-31 2003-05-22 Henkel Kgaa Neue Alkalische Protease-Varianten und Wasch- und Reinigungsmittel enthaltend diese neuen Alkalischen Protease-Varianten
BR0306956A (pt) * 2002-01-16 2006-04-11 Genencor Int variantes de protease multiplamente substituìdas
US7888093B2 (en) * 2002-11-06 2011-02-15 Novozymes A/S Subtilase variants
US7294499B2 (en) 2003-01-30 2007-11-13 Novozymes A/S Subtilases
US7727756B2 (en) * 2003-03-21 2010-06-01 Novozymes A/S Subtilases
CN1784490A (zh) * 2003-05-07 2006-06-07 诺和酶股份有限公司 变体枯草杆菌蛋白酶(枯草杆菌酶)
EP3887515A1 (en) * 2018-11-28 2021-10-06 Danisco US Inc. Subtilisin variants having improved stability
EP4525615A2 (en) 2022-05-14 2025-03-26 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4760025A (en) * 1984-05-29 1988-07-26 Genencor, Inc. Modified enzymes and methods for making same
US5155033A (en) * 1989-01-06 1992-10-13 Genencor, Inc. Subtilisins modified at position 225 resulting in a shift in catalytic activity
WO1987004461A1 (en) * 1986-01-15 1987-07-30 Amgen THERMALLY STABLE AND pH STABLE SUBTILISIN ANALOGS AND METHOD FOR PRODUCTION THEREOF
US5013657A (en) * 1988-04-12 1991-05-07 Bryan Philip N Subtilisin mutations
US4980288A (en) * 1986-02-12 1990-12-25 Genex Corporation Subtilisin with increased thermal stability
US4990452A (en) * 1986-02-12 1991-02-05 Genex Corporation Combining mutations for stabilization of subtilisin
ES2068181T3 (es) * 1986-04-30 1995-04-16 Genencor Int Mutantes de la carbonil hidrolasa no humana, secuencias de adn y vectores que codifican las mismas y huespedes transformados con dichos vectores.
NZ221627A (en) * 1986-09-09 1993-04-28 Genencor Inc Preparation of enzymes, modifications, catalytic triads to alter ratios or transesterification/hydrolysis ratios
EP0283075B1 (en) * 1987-02-27 1999-05-12 Genencor International, Inc. Molecular cloning and expression of genes encoding proteolytic enzymes
ATE184316T1 (de) * 1987-04-06 1999-09-15 Novo Nordisk As Regelung von elektrostatischen interaktionen an metallionen-bindungsstellen zur stabilisierung von proteinen
US4914031A (en) * 1987-04-10 1990-04-03 Amgen, Inc. Subtilisin analogs
FR2614467A1 (fr) * 1987-04-21 1988-10-28 Telemecanique Electrique Appareil interrupteur de protection a mecanisme de declenchement simplifie

Also Published As

Publication number Publication date
DE69028040D1 (de) 1996-09-19
DE69028040T2 (de) 1997-02-27
KR920701426A (ko) 1992-08-11
EP0398539B1 (en) 1996-08-14
AU618675B2 (en) 1992-01-02
WO1990014420A1 (en) 1990-11-29
US5397705A (en) 1995-03-14
KR100188532B1 (ko) 1999-06-01
AU5558090A (en) 1990-12-18
HK1006857A1 (en) 1999-03-19
EP0398539A1 (en) 1990-11-22
SG47797A1 (en) 1998-04-17
GR3021010T3 (en) 1996-12-31
CA2016211C (en) 1998-07-21
DK0398539T3 (da) 1996-12-09
ATE141324T1 (de) 1996-08-15
CA2016211A1 (en) 1990-11-17
JPH03505976A (ja) 1991-12-26

Similar Documents

Publication Publication Date Title
ES2091224T3 (es) Subtilisinas con mutaciones multiples.
DE3853328D1 (de) Subtilisin-analoge.
ES2125224T3 (es) Mutantes de subtilisina.
ATE82281T1 (de) Aryl-substituierte(n-piperidinyl)methyl und (npiperazinyl)methylazole mit antipsychotischer wirkung.
DE3469916D1 (en) Compositions containing odor purified proteolytic enzymes and perfumes
ES2090857T3 (es) Composiciones para el cuidado del cabello.
BR9407834A (pt) Composições de limpeza contendo protease
TR24655A (tr) Karbon isleri ve karbon islerini iceren kaucuk bilesimleri
IL99553A0 (en) Compositions containing oligonucleotides linked to expression elements,a kit for the preparation of vectors useful for the expression of a diverse population of random peptides and methods utilizing the same
DE3785570D1 (de) Zusammensetzungen enthaltend lhrh-analoga.
CA2050212A1 (en) Hair treatment composition
EP0441460A3 (en) Cyclopentenes, cyclopentadienes, their manufacture and lubricating compositions containing them
EP0428342A3 (en) Stable thiol-ene compositions
DE69312499D1 (de) Kautschukzusammensetzungen enthaltend epoxidierten naturkautschuk und naturkautschuk
ATE110772T1 (de) Mutierter t-pa mit kringle-ersatz.
MY100883A (en) The use of salicylic acid esters as perfumes, perfume compositions containing them and new salicylic esters.
FI863078A0 (fi) Kontinuerligt frigoerande peptidkompositioner.
GB8827466D0 (en) Organopolysiloxane composition curable to elastomer & use thereof
DE3888543D1 (de) Zusammensetzungen zur herstellung von gasdurchlässigen kontaktlinsen.
RO103459B1 (en) Rubber composition for garnitures of bellows type
AU4405689A (en) New limphokines, dna sequences coding for said limphokines and pharmaceutical compositions containing said limphokines
ES2027108A6 (es) Nuevo protector dental.

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 398539

Country of ref document: ES