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GB1248764A - Antibodies against reagin-immunoglobulins (reagin-ig), and their use - Google Patents

Antibodies against reagin-immunoglobulins (reagin-ig), and their use

Info

Publication number
GB1248764A
GB1248764A GB4180168A GB4180168A GB1248764A GB 1248764 A GB1248764 A GB 1248764A GB 4180168 A GB4180168 A GB 4180168A GB 4180168 A GB4180168 A GB 4180168A GB 1248764 A GB1248764 A GB 1248764A
Authority
GB
United Kingdom
Prior art keywords
reagin
reagent
labelled
antibodies
allergen
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB4180168A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pfizer Health AB
Original Assignee
Pharmacia AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pharmacia AB filed Critical Pharmacia AB
Priority to GB345771A priority Critical patent/GB1248765A/en
Publication of GB1248764A publication Critical patent/GB1248764A/en
Expired legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/001—Preparation for luminescence or biological staining
    • A61K49/0013—Luminescence
    • A61K49/0017—Fluorescence in vivo
    • A61K49/005—Fluorescence in vivo characterised by the carrier molecule carrying the fluorescent agent
    • A61K49/0058—Antibodies
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/001—Preparation for luminescence or biological staining
    • A61K49/0013—Luminescence
    • A61K49/0017—Fluorescence in vivo
    • A61K49/0019—Fluorescence in vivo characterised by the fluorescent group, e.g. oligomeric, polymeric or dendritic molecules
    • A61K49/0021—Fluorescence in vivo characterised by the fluorescent group, e.g. oligomeric, polymeric or dendritic molecules the fluorescent group being a small organic molecule
    • A61K49/0041—Xanthene dyes, used in vivo, e.g. administered to a mice, e.g. rhodamines, rose Bengal
    • A61K49/0043—Fluorescein, used in vivo
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K51/00—Preparations containing radioactive substances for use in therapy or testing in vivo
    • A61K51/02—Preparations containing radioactive substances for use in therapy or testing in vivo characterised by the carrier, i.e. characterised by the agent or material covalently linked or complexing the radioactive nucleus
    • A61K51/04—Organic compounds
    • A61K51/08—Peptides, e.g. proteins, carriers being peptides, polyamino acids, proteins
    • A61K51/10—Antibodies or immunoglobulins; Fragments thereof, the carrier being an antibody, an immunoglobulin or a fragment thereof, e.g. a camelised human single domain antibody or the Fc fragment of an antibody
    • A61K51/1015—Antibodies or immunoglobulins; Fragments thereof, the carrier being an antibody, an immunoglobulin or a fragment thereof, e.g. a camelised human single domain antibody or the Fc fragment of an antibody against material from plants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K51/00—Preparations containing radioactive substances for use in therapy or testing in vivo
    • A61K51/02—Preparations containing radioactive substances for use in therapy or testing in vivo characterised by the carrier, i.e. characterised by the agent or material covalently linked or complexing the radioactive nucleus
    • A61K51/04—Organic compounds
    • A61K51/08—Peptides, e.g. proteins, carriers being peptides, polyamino acids, proteins
    • A61K51/10—Antibodies or immunoglobulins; Fragments thereof, the carrier being an antibody, an immunoglobulin or a fragment thereof, e.g. a camelised human single domain antibody or the Fc fragment of an antibody
    • A61K51/1018—Antibodies or immunoglobulins; Fragments thereof, the carrier being an antibody, an immunoglobulin or a fragment thereof, e.g. a camelised human single domain antibody or the Fc fragment of an antibody against material from animals or humans
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K51/00—Preparations containing radioactive substances for use in therapy or testing in vivo
    • A61K51/02—Preparations containing radioactive substances for use in therapy or testing in vivo characterised by the carrier, i.e. characterised by the agent or material covalently linked or complexing the radioactive nucleus
    • A61K51/04—Organic compounds
    • A61K51/08—Peptides, e.g. proteins, carriers being peptides, polyamino acids, proteins
    • A61K51/10—Antibodies or immunoglobulins; Fragments thereof, the carrier being an antibody, an immunoglobulin or a fragment thereof, e.g. a camelised human single domain antibody or the Fc fragment of an antibody
    • A61K51/1078—Antibodies or immunoglobulins; Fragments thereof, the carrier being an antibody, an immunoglobulin or a fragment thereof, e.g. a camelised human single domain antibody or the Fc fragment of an antibody the antibody being against an immunoglobulin, i.e. being an (anti)-anti-idiotypic antibody
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/16—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from plants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/42—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against immunoglobulins
    • C07K16/4283—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against immunoglobulins against an allotypic or isotypic determinant on Ig
    • C07K16/4291—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against immunoglobulins against an allotypic or isotypic determinant on Ig against IgE
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2121/00—Preparations for use in therapy
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2123/00—Preparations for testing in vivo

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Biochemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Optics & Photonics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Botany (AREA)
  • Endocrinology (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Peptides Or Proteins (AREA)

Abstract

1,248,764. Determination of reagin-Ig using labelled antibodies. PHARMACIA AB. 3 Sept., 1968 [6 Sept., 1967], No. 41801/68. Heading B1X. Labelled antibodies against reagin Ig or against fragments of reagin Ig are used to determine reagin Ig (reagins are antibodies against allergens). The antibodies may be labelled with a radioactive isotope, for example I<SP>125</SP>, or with a fluorescent group using for example fluorescein isothiocyanate. Means for determining reagins comprises a first reagent comprising a water-insoluble polymer having bound to it allergen against which the reagin to be determined is directed and a second reagent comprising the labelled antibodies as above. The sample is incubated with the first reagent when any reagin present in the sample becomes attached to the polymer particles; the polymer particles are separated from the liquid phase, washed and then incubated with the second reagent. The solid and liquid phases are separated again and the radiation in either phase determined and compared with that in a control. The water-insoluble polymer is preferably particulate but may be in the form of discs, strips or a test tube wall and may be a copolymer of a polyhydroxy compound for example dextran, starch, dextrins, polyvinylalcohol with a bifunctional substance. The allergen may be physically or chemically bound to the polymer. Examples relate to Sephadex (RTM) as carrier, birch pollen as allergen and I<SP>125</SP>-labelled rabbit antibody.
GB4180168A 1967-09-06 1968-09-03 Antibodies against reagin-immunoglobulins (reagin-ig), and their use Expired GB1248764A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB345771A GB1248765A (en) 1967-09-06 1968-09-03 Reagin-immunoglobulins (reagin-ig) and their use

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE1229767A SE341239B (en) 1967-09-06 1967-09-06

Publications (1)

Publication Number Publication Date
GB1248764A true GB1248764A (en) 1971-10-06

Family

ID=20295495

Family Applications (1)

Application Number Title Priority Date Filing Date
GB4180168A Expired GB1248764A (en) 1967-09-06 1968-09-03 Antibodies against reagin-immunoglobulins (reagin-ig), and their use

Country Status (13)

Country Link
JP (1) JPS50892B1 (en)
AT (2) AT288593B (en)
BE (1) BE720341A (en)
CH (1) CH534878A (en)
CS (1) CS191857B2 (en)
DK (1) DK131400B (en)
ES (1) ES357863A1 (en)
FI (1) FI48785C (en)
FR (1) FR1588874A (en)
GB (1) GB1248764A (en)
NL (1) NL162209C (en)
NO (2) NO127685B (en)
SE (1) SE341239B (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3966897A (en) * 1973-04-02 1976-06-29 Marine Colloids, Inc. Medium for use in bioassay and method of using same
US4054646A (en) 1973-07-30 1977-10-18 General Electric Method and apparatus for detection of antibodies and antigens
US4289748A (en) * 1979-05-31 1981-09-15 United States Of America Ultrasensitive enzymatic radioimmunoassay method
US4481298A (en) * 1981-04-13 1984-11-06 Amf Incorporated Pre-precipitated double antibody immunoassay method

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USRE31006E (en) * 1968-09-24 1982-08-03 Akzona Incorporated Process for the demonstration and determination of reaction components having specific binding affinity for each other
NL154600B (en) * 1971-02-10 1977-09-15 Organon Nv METHOD FOR THE DETERMINATION AND DETERMINATION OF SPECIFIC BINDING PROTEINS AND THEIR CORRESPONDING BINDABLE SUBSTANCES.
US4041146A (en) * 1975-05-01 1977-08-09 General Electric Company Method for detection of biological particles
GB1555142A (en) * 1975-08-14 1979-11-07 Sinai School Medicine Blood cell typing and compatibility test procedure
AT343822B (en) * 1976-08-20 1978-06-26 Immuno Ag RADIOIMMUNOLOGICAL METHOD AND EQUIPMENT FOR DETERMINING ANTIGENES
FR2502786B1 (en) * 1981-03-24 1985-06-21 Stallergenes Laboratoire METHOD FOR FIXING ANTIGENS AND ANTIBODIES TO POLYSACCHARIDE SUPPORT, AND USE OF THE PRODUCT OBTAINED THEREFOR FOR IMMUNOASSAYS
NL8102178A (en) * 1981-05-02 1982-12-01 Stichting Centraal Lab METHOD FOR DEMONSTRATING ANTI-ANTIGENS AGAINST CERTAIN ANTIGENS, EQUIPMENT AND REAGENTS FOR CARRYING OUT THIS METHOD
JPS6199195U (en) * 1984-11-30 1986-06-25

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3966897A (en) * 1973-04-02 1976-06-29 Marine Colloids, Inc. Medium for use in bioassay and method of using same
US4054646A (en) 1973-07-30 1977-10-18 General Electric Method and apparatus for detection of antibodies and antigens
US4289748A (en) * 1979-05-31 1981-09-15 United States Of America Ultrasensitive enzymatic radioimmunoassay method
US4481298A (en) * 1981-04-13 1984-11-06 Amf Incorporated Pre-precipitated double antibody immunoassay method

Also Published As

Publication number Publication date
FI48785C (en) 1974-12-10
CS191857B2 (en) 1979-07-31
NL162209B (en) 1979-11-15
NO138182B (en) 1978-04-10
DK131400B (en) 1975-07-07
FI48785B (en) 1974-09-02
AT288593B (en) 1971-03-10
JPS50892B1 (en) 1975-01-13
ES357863A1 (en) 1970-04-01
CH534878A (en) 1973-03-15
NO138182C (en) 1978-07-26
NL162209C (en) 1980-04-15
DK131400C (en) 1975-11-24
FR1588874A (en) 1970-03-16
SE341239B (en) 1971-12-20
NO127685B (en) 1973-07-30
NL6812559A (en) 1969-03-10
BE720341A (en) 1969-02-17
DE1798170B1 (en) 1972-04-27
AT295744B (en) 1972-01-10

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