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FR3128231B1 - Procédé et dispositif de dessalage et de concentration ou d’analyse d’un échantillon d’acides nucléiques - Google Patents

Procédé et dispositif de dessalage et de concentration ou d’analyse d’un échantillon d’acides nucléiques Download PDF

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Publication number
FR3128231B1
FR3128231B1 FR2110921A FR2110921A FR3128231B1 FR 3128231 B1 FR3128231 B1 FR 3128231B1 FR 2110921 A FR2110921 A FR 2110921A FR 2110921 A FR2110921 A FR 2110921A FR 3128231 B1 FR3128231 B1 FR 3128231B1
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France
Prior art keywords
sample
flow
nucleic acid
analysis
desalination
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FR2110921A
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English (en)
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FR3128231A1 (fr
Inventor
Frédéric Ginot
Audrey Boutonnet
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Adelis
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Adelis
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Publication date
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Priority to FR2110921A priority Critical patent/FR3128231B1/fr
Priority to EP22803197.7A priority patent/EP4392575A1/fr
Priority to PCT/EP2022/078580 priority patent/WO2023062162A1/fr
Priority to US18/697,742 priority patent/US20240401115A1/en
Publication of FR3128231A1 publication Critical patent/FR3128231A1/fr
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Publication of FR3128231B1 publication Critical patent/FR3128231B1/fr
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0654—Lenses; Optical fibres
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0421—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic electrophoretic flow
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G01N2030/8827—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving nucleic acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Genetics & Genomics (AREA)
  • Fluid Mechanics (AREA)
  • Dispersion Chemistry (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

TITRE DE L’INVENTION : PROCÉDÉ ET DISPOSITIF DE DESSALAGE ET DE CONCENTRATION OU D’ANALYSE D’UN ÉCHANTILLON D’ACIDES NUCLÉIQUES Le procédé de dessalage et de concentration d’un échantillon d’acides nucléiques plus conducteur qu’un tampon d’analyse, comporte, au moins une itération d’une alternance :- d’une étape d’écoulement laminaire de l’échantillon dans un capillaire muni d’une restriction locale de sa section, dans un premier sens d’écoulement, pendant laquelle l’échantillon est soumis à une première différence de potentiel électrique dont l’action sur les molécules d’acides nucléiques est opposée au premier sens d’écoulement et provoque la retenue de molécules d’acides nucléiques dans le capillaire,- d’une étape d’écoulement laminaire de l’échantillon, dans un deuxième sens d’écoulement opposé au premier sens d’écoulement. Figure pour l’abrégé : Figure 10.
FR2110921A 2021-10-14 2021-10-14 Procédé et dispositif de dessalage et de concentration ou d’analyse d’un échantillon d’acides nucléiques Active FR3128231B1 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
FR2110921A FR3128231B1 (fr) 2021-10-14 2021-10-14 Procédé et dispositif de dessalage et de concentration ou d’analyse d’un échantillon d’acides nucléiques
EP22803197.7A EP4392575A1 (fr) 2021-10-14 2022-10-13 Procédé et dispositif de dessalage et de concentration ou d'analyse d'un échantillon d'acides nucléiques
PCT/EP2022/078580 WO2023062162A1 (fr) 2021-10-14 2022-10-13 Procédé et dispositif de dessalage et de concentration ou d'analyse d'un échantillon d'acides nucléiques
US18/697,742 US20240401115A1 (en) 2021-10-14 2022-10-13 Method and device for desalting and concentrating or analysing a sample of nucleic acids

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR2110921A FR3128231B1 (fr) 2021-10-14 2021-10-14 Procédé et dispositif de dessalage et de concentration ou d’analyse d’un échantillon d’acides nucléiques
FR2110921 2021-10-14

Publications (2)

Publication Number Publication Date
FR3128231A1 FR3128231A1 (fr) 2023-04-21
FR3128231B1 true FR3128231B1 (fr) 2024-10-25

Family

ID=80595394

Family Applications (1)

Application Number Title Priority Date Filing Date
FR2110921A Active FR3128231B1 (fr) 2021-10-14 2021-10-14 Procédé et dispositif de dessalage et de concentration ou d’analyse d’un échantillon d’acides nucléiques

Country Status (4)

Country Link
US (1) US20240401115A1 (fr)
EP (1) EP4392575A1 (fr)
FR (1) FR3128231B1 (fr)
WO (1) WO2023062162A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR3158968A1 (fr) 2024-02-01 2025-08-08 Adelis Procédé de prédiction de la réponse d’un patient atteint de cancer à un traitement d’immunothérapie

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2994103B1 (fr) 2012-08-03 2016-05-27 Centre Nat Rech Scient Procede de separation de molecules en solution
FR3038718B1 (fr) 2015-07-10 2022-04-29 Picometrics Tech Systeme de concentration, preconcentration par empilement d'echantillon et/ou purification pour analyse

Also Published As

Publication number Publication date
US20240401115A1 (en) 2024-12-05
WO2023062162A1 (fr) 2023-04-20
FR3128231A1 (fr) 2023-04-21
EP4392575A1 (fr) 2024-07-03

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