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FR3116065B1 - Detection of nucleic acids in a biological sample - Google Patents

Detection of nucleic acids in a biological sample Download PDF

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Publication number
FR3116065B1
FR3116065B1 FR2011611A FR2011611A FR3116065B1 FR 3116065 B1 FR3116065 B1 FR 3116065B1 FR 2011611 A FR2011611 A FR 2011611A FR 2011611 A FR2011611 A FR 2011611A FR 3116065 B1 FR3116065 B1 FR 3116065B1
Authority
FR
France
Prior art keywords
nucleic acids
detection
biological sample
protease
quantification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
FR2011611A
Other languages
French (fr)
Other versions
FR3116065A1 (en
Inventor
Philippe Pourquier
Benjamin Sauldé
Anaïs Bresson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Innovative Diagnostics Genetics
Original Assignee
Innovative Diagnostics Genetics
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Innovative Diagnostics Genetics filed Critical Innovative Diagnostics Genetics
Priority to FR2011611A priority Critical patent/FR3116065B1/en
Priority to EP21819923.0A priority patent/EP4244378A1/en
Priority to PCT/FR2021/051994 priority patent/WO2022101583A1/en
Publication of FR3116065A1 publication Critical patent/FR3116065A1/en
Application granted granted Critical
Publication of FR3116065B1 publication Critical patent/FR3116065B1/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne un procédé in vitro de libération, de détection et/ou quantification d’acides nucléiques d’un échantillon biologique, comprenant les étapes suivantes : a) lyse et dénaturation protéique de l’échantillon par une protéase, b) inactivation de la protéase par un inhibiteur de ladite protéase, c) optionnellement amplification des acides nucléiques présents dans le lysat, et d) éventuellement détection et/ou quantification desdits acides nucléiques.The present invention relates to an in vitro method for the release, detection and/or quantification of nucleic acids from a biological sample, comprising the following steps: a) lysis and protein denaturation of the sample by a protease, b) inactivation of the protease by an inhibitor of said protease, c) optionally amplification of the nucleic acids present in the lysate, and d) optionally detection and/or quantification of said nucleic acids.

FR2011611A 2020-11-12 2020-11-12 Detection of nucleic acids in a biological sample Active FR3116065B1 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
FR2011611A FR3116065B1 (en) 2020-11-12 2020-11-12 Detection of nucleic acids in a biological sample
EP21819923.0A EP4244378A1 (en) 2020-11-12 2021-11-10 Method for detecting nucleic acids in a biological sample
PCT/FR2021/051994 WO2022101583A1 (en) 2020-11-12 2021-11-10 Method for detecting nucleic acids in a biological sample

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR2011611 2020-11-12
FR2011611A FR3116065B1 (en) 2020-11-12 2020-11-12 Detection of nucleic acids in a biological sample

Publications (2)

Publication Number Publication Date
FR3116065A1 FR3116065A1 (en) 2022-05-13
FR3116065B1 true FR3116065B1 (en) 2024-11-29

Family

ID=75339810

Family Applications (1)

Application Number Title Priority Date Filing Date
FR2011611A Active FR3116065B1 (en) 2020-11-12 2020-11-12 Detection of nucleic acids in a biological sample

Country Status (3)

Country Link
EP (1) EP4244378A1 (en)
FR (1) FR3116065B1 (en)
WO (1) WO2022101583A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116334182A (en) * 2023-03-29 2023-06-27 南京健邦锦源医疗科技有限公司 An inactivated virus preservation solution that can be stored at room temperature

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2163652B2 (en) * 2002-01-28 2023-05-31 Life Technologies Corporation Preparing crude biological extracts using protease, suitable for preparing cDNA
US8211637B2 (en) * 2008-12-19 2012-07-03 Life Technologies Corporation Proteinase K inhibitors, methods and compositions therefor
WO2018217912A1 (en) * 2017-05-23 2018-11-29 President And Fellows Of Harvard College Multiplex end-tagging amplification of nucleic acids

Also Published As

Publication number Publication date
FR3116065A1 (en) 2022-05-13
EP4244378A1 (en) 2023-09-20
WO2022101583A1 (en) 2022-05-19

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