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FR3032719B1 - DIAGNOSTIC DEVICE BY ISOTHERMAL DNA AMPLIFICATION - Google Patents

DIAGNOSTIC DEVICE BY ISOTHERMAL DNA AMPLIFICATION Download PDF

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Publication number
FR3032719B1
FR3032719B1 FR1551189A FR1551189A FR3032719B1 FR 3032719 B1 FR3032719 B1 FR 3032719B1 FR 1551189 A FR1551189 A FR 1551189A FR 1551189 A FR1551189 A FR 1551189A FR 3032719 B1 FR3032719 B1 FR 3032719B1
Authority
FR
France
Prior art keywords
zone
diagnostic
sample
dna amplification
diagnostic device
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
FR1551189A
Other languages
French (fr)
Other versions
FR3032719A1 (en
Inventor
Laura Magro
Fabrice Monti
Patrick Tabeling
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centre National de la Recherche Scientifique CNRS
Ecole Superieure de Physique et Chimie Industrielles de Ville de Paris ESPCI
Original Assignee
Centre National de la Recherche Scientifique CNRS
Ecole Superieure de Physique et Chimie Industrielles de Ville de Paris ESPCI
ESPCI Innov SAS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centre National de la Recherche Scientifique CNRS, Ecole Superieure de Physique et Chimie Industrielles de Ville de Paris ESPCI, ESPCI Innov SAS filed Critical Centre National de la Recherche Scientifique CNRS
Priority to FR1551189A priority Critical patent/FR3032719B1/en
Priority to US15/550,716 priority patent/US20180245144A1/en
Priority to EP16705099.6A priority patent/EP3256253A1/en
Priority to PCT/EP2016/053077 priority patent/WO2016128570A1/en
Publication of FR3032719A1 publication Critical patent/FR3032719A1/en
Application granted granted Critical
Publication of FR3032719B1 publication Critical patent/FR3032719B1/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0636—Integrated biosensor, microarrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/12—Specific details about materials
    • B01L2300/126—Paper
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1827—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using resistive heater
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Dispersion Chemistry (AREA)
  • Hematology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Le dispositif (10) de diagnostic par amplification isotherme d'ADN comporte : - un substrat (105) poreux comportant : - une zone (110) comportant au moins un composé (115) réactif réagissant avec un composé (120) cible d'un échantillon, dite « zone de diagnostic », - une zone (125) de dépôt de fluide transporté, par capillarité, dans la zone de dépôt d'échantillon et dans la zone de diagnostic, - la zone (130) de dépôt d'échantillon positionnée entre la zone de dépôt de fluide et la zone de diagnostic, et - deux canaux (135, 140), positionnés dans l'épaisseur du substrat poreux, respectivement entre, d'une part, la zone de dépôt de fluide et la zone de dépôt d'échantillon, d'autre part, la zone de dépôt d'échantillon et la zone de diagnostic ; et - un moyen (205) d'échauffement isotherme local la zone de diagnostic.The isothermal DNA amplification diagnostic device (10) comprises: - a porous substrate (105) comprising: - a zone (110) comprising at least one reactive compound (115) reacting with a target compound (120) of a sample, known as the “diagnostic zone”, - a zone (125) for depositing transported fluid, by capillary action, in the sample deposit zone and in the diagnostic zone, - the sample deposit zone (130) positioned between the fluid deposition zone and the diagnostic zone, and - two channels (135, 140), positioned in the thickness of the porous substrate, respectively between, on the one hand, the fluid deposition zone and the zone on the other hand, the sample deposition area and the diagnostic area; and - a means (205) of local isothermal heating of the diagnostic zone.

FR1551189A 2015-02-13 2015-02-13 DIAGNOSTIC DEVICE BY ISOTHERMAL DNA AMPLIFICATION Expired - Fee Related FR3032719B1 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
FR1551189A FR3032719B1 (en) 2015-02-13 2015-02-13 DIAGNOSTIC DEVICE BY ISOTHERMAL DNA AMPLIFICATION
US15/550,716 US20180245144A1 (en) 2015-02-13 2016-02-12 Paper device for genetic diagnosis
EP16705099.6A EP3256253A1 (en) 2015-02-13 2016-02-12 Paper device for genetic diagnosis
PCT/EP2016/053077 WO2016128570A1 (en) 2015-02-13 2016-02-12 Paper device for genetic diagnosis

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR1551189A FR3032719B1 (en) 2015-02-13 2015-02-13 DIAGNOSTIC DEVICE BY ISOTHERMAL DNA AMPLIFICATION
FR1551189 2015-02-13

Publications (2)

Publication Number Publication Date
FR3032719A1 FR3032719A1 (en) 2016-08-19
FR3032719B1 true FR3032719B1 (en) 2020-05-15

Family

ID=52829148

Family Applications (1)

Application Number Title Priority Date Filing Date
FR1551189A Expired - Fee Related FR3032719B1 (en) 2015-02-13 2015-02-13 DIAGNOSTIC DEVICE BY ISOTHERMAL DNA AMPLIFICATION

Country Status (1)

Country Link
FR (1) FR3032719B1 (en)

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6379929B1 (en) * 1996-11-20 2002-04-30 The Regents Of The University Of Michigan Chip-based isothermal amplification devices and methods
GB0919159D0 (en) * 2009-11-02 2009-12-16 Sec Dep For Environment Food A Device and apparatus
US20140295415A1 (en) * 2011-11-04 2014-10-02 Diagnostics For All, Inc. Low cost, disposable molecular diagnostic devices

Also Published As

Publication number Publication date
FR3032719A1 (en) 2016-08-19

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