[go: up one dir, main page]

FR2682965A1 - PRODUCTION OF BONE AND PEARL FROM CULTURE OF BONE FORMING CELLS. - Google Patents

PRODUCTION OF BONE AND PEARL FROM CULTURE OF BONE FORMING CELLS. Download PDF

Info

Publication number
FR2682965A1
FR2682965A1 FR9113083A FR9113083A FR2682965A1 FR 2682965 A1 FR2682965 A1 FR 2682965A1 FR 9113083 A FR9113083 A FR 9113083A FR 9113083 A FR9113083 A FR 9113083A FR 2682965 A1 FR2682965 A1 FR 2682965A1
Authority
FR
France
Prior art keywords
bone
pearl
forming cells
fragments
nacre
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
FR9113083A
Other languages
French (fr)
Inventor
Camprasse Georges
Camprasse Serge
Lopez-Rabereau Evelyne
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to FR9113083A priority Critical patent/FR2682965A1/en
Priority to PCT/FR1992/001008 priority patent/WO1993008265A2/en
Priority to AU29487/92A priority patent/AU2948792A/en
Publication of FR2682965A1 publication Critical patent/FR2682965A1/en
Withdrawn legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A44—HABERDASHERY; JEWELLERY
    • A44C—PERSONAL ADORNMENTS, e.g. JEWELLERY; COINS
    • A44C17/00—Gems or the like
    • A44C17/007—Special types of gems
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0654—Osteocytes, Osteoblasts, Odontocytes; Bones, Teeth
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/10—Mineral substrates
    • C12N2533/18—Calcium salts, e.g. apatite, Mineral components from bones, teeth, shells

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Transplantation (AREA)
  • Botany (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Dermatology (AREA)
  • Medicinal Chemistry (AREA)
  • Rheumatology (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Farming Of Fish And Shellfish (AREA)
  • Materials For Medical Uses (AREA)

Abstract

According to the invention the artificial fabrication method for fabricating simultaneously or separately bone and nacre is implemented by the activity of osteonacrigenic factors contained in nacre. The method according to the invention consists in using fragments of nacre or powder of nacre resulting from the test of bivalve aquatic Mollusca Gasteropoda or Cephalopoda in the "in vitro" or "in vivo" presence of bone cells or bone-forming cells or cells susceptible of becoming bone-forming cells, associated to one or a plurality of bone fragments. The method according to the invention may be implemented in molds of various shapes and sizes, whereafter the bone parts thus obtained may be used as grafts in bone surgery and the nacre may be used to produce pearls, jewels or objects.

Description

PRODUCTION D'OS ET DE PERLE A PARTIR DE CULTURE DE
CELLULES FORMATRICES D'OS
La présente invention concerne un procédé de production d'os naturel et de nacre sous forme de perle à partir de cellules formatrices d'os et de biocristaux provenant du test nacré de mollusques aquatiques.BONE AND PEARL PRODUCTION FROM CULTURE OF
BONE FORMAT CELLS
The present invention relates to a process for producing natural bone and pearl in the form of a pearl from bone-forming cells and biocrystals originating from the pearly test for aquatic molluscs.

Jusqutà ce jour on n' a jamais pu produire de manière artificielle en quantité suffisante du tissu osseux d'origine animale ou humaine destiné à servir de greffon. Les circonstances chirurgicales pouvant donner lieu à la nécessité d'effectuer des greffes osseuses sont nombreuses : chirurgie orthopédique - chirurgie réparatrice - chirurgie plastique - carcinologique etc...Dans tous les cas les greffons proviennent soit du patient lui-même et dans ce cas on pratique une autogreffe, soit d'une banque d'os constituée de pièces osseuses traitées de façon à les débarrasser de leur partie organique pouvant provoquer des réactions immunitaires, stérilisées par rayonnement ionisant et congelées, on pratique alors une hétérogreffe. Until now, it has never been possible to artificially produce a sufficient quantity of bone tissue of animal or human origin intended to serve as a graft. The surgical circumstances which can give rise to the need to perform bone grafts are numerous: orthopedic surgery - restorative surgery - plastic surgery - carcinology etc ... In all cases the grafts come either from the patient himself and in this case we performs an autograft, that is to say a bone bank made up of bone pieces treated so as to rid them of their organic part which can cause immune reactions, sterilized by ionizing radiation and frozen, a heterograft is then practiced.

Ces pièces osseuses, une fois greffées constituent une trame minérale dans laquelle se développera un processus de néoformation osseuse.These bone pieces, once grafted, constitute a mineral framework in which a process of bone neoformation will develop.

De la-3nêne façon l'obtention de perle de culture se fait à partir de fragments de nacre que l'on introduit dans un organe spécifique du mollusque vivant. L'inclusion d'un tel fragment produit un phénomène irritatif qui lui même induit un' processus de minéralisation par apposition de biocristaux autour du fragment de nacre, les perlés ainsi obtenues étant obligatoirement de forme arrondie.In this way, the cultured pearl is obtained from fragments of mother-of-pearl which are introduced into a specific organ of the living mollusk. The inclusion of such a fragment produces an irritant phenomenon which itself induces a mineralization process by affixing biocrystals around the fragment of mother-of-pearl, the pearls thus obtained are necessarily of rounded shape.

Les inconvénients liés aux greffes osseuses sont de plusieurs ordres, toutefois les plus importantes sont les difficultés d'approvisionnement et de stockage ainsi que les réactions immunitaires de rejet dans les cas d'hétérogreffes. Dans le cas d'autogreffes l'inconvénient majeur réside dans le fait que l'on ne peut disposer de fragment osseux de volume suffisant lorsqu'il sagit de remplacer une grande perte de substance.The disadvantages linked to bone grafts are of several orders, however the most important are the difficulties of supply and storage as well as the immune reactions of rejection in cases of heterografts. In the case of autografts the major drawback lies in the fact that one cannot have a bone fragment of sufficient volume when it comes to replacing a great loss of substance.

C'est la raison pour laquelle certains inventeurs ont proposé des pièces osseuses réalisées dans des biomatériaux naturels biologiquement compatibles tel que le corail. Ces biomatériaux ne sont en fait que des réserves de calcium occupant le volume laissé vide par la perte de substance et qui peu à peu sera remplacé par de l'os néoformé.This is the reason why certain inventors have proposed bone pieces produced in biologically compatible natural biomaterials such as coral. These biomaterials are in fact only calcium reserves occupying the volume left empty by the loss of substance and which little by little will be replaced by newly formed bone.

Le problème majeur vient du fait que le pouvoir ostéogénique des cellules osseuses entrant dans le processus de minéralisation s'épuise, limitant de ce fait la colonisation totale du greffon de biomatériau. The major problem comes from the fact that the osteogenic power of the bone cells entering the mineralization process is exhausted, thereby limiting the total colonization of the biomaterial graft.

D'autre inventeurs ont proposé comme substitut de pièces osseuses des éléments usinés à partir du test nacré de certains mollusques mais là aussi la taille et le volume limités de ces pièces usinées les rend impropres à une utilisation dans le remplacement des pertes de substances de la plupart des os longs du squelette.Other inventors have proposed, as a substitute for bone parts, elements machined from the pearly test of certain molluscs, but here again the limited size and volume of these machined parts makes them unsuitable for use in replacing losses of substances from the most of the long bones of the skeleton.

C'est pour ces raisons que nous avons expérimenté un procédé artificiel simple de production simultannée ou séparée de tissu osseux et de perle de culture.It is for these reasons that we have experimented with a simple artificial process for the simultaneous or separate production of bone tissue and cultured pearl.

Le procédé selon l'invention consiste à mettre en oeuvre un processus histochimique destiné à produire artificiellement dans le même temps ou séparémment d'une part de l'os d'origine animale ou humaine et d'autre part de la perle de culture.The method according to the invention consists in implementing a histochemical process intended to produce artificially at the same time or separately on the one hand from bone of animal or human origin and on the other hand from cultured pearl.

Le procédé selon l'invention consiste à mettre en présence dans un milieu de culture approprié une couche de cellules formatrices d'os (ostéoblastes), des particules ou de la poudre de biocristaux de nacre. Les cellules formatrices d'os seront de préférence issues d'explants de jeunes mamifères ou de fragments d'os humains sans que cet exemple soit limitatif. Les biocristaux proviennent du test nacré de mollusques aquatiques bivalves, gasté ropodes, ou cephalopodes.The method according to the invention consists in bringing into a suitable culture medium a layer of bone-forming cells (osteoblasts), particles or powder of mother-of-pearl biocrystals. The bone-forming cells will preferably be derived from explants of young mammals or from fragments of human bone without this example being limiting. The biocrystals come from the pearly test of bivalve aquatic molluscs, gastropods, or cephalopods.

Le procédé selon l'invention est mis en oeuvre dans des modules de taille et de forme variables, ces modules serviront de moules dans lesquels se développera le procédé selon l'invention afin d'en obtenir des répliques en os ou en perle.The method according to the invention is implemented in modules of variable size and shape, these modules will serve as molds in which the method according to the invention will develop in order to obtain replicas of bone or pearl.

Selon un mode de réalisation préférentielle le procédé selon l'invention peut servir à la fabrication-de pièces osseuses destinées à servir de greffon en chirurgie osseuse ; et à la réali sation d'objets ou de bijoux en perle de forme ét de dimension variables sans que cela soit limitatif.According to a preferred embodiment, the method according to the invention can be used for the manufacture of bone pieces intended to serve as grafts in bone surgery; and the production of objects or pearl jewelry of variable shape and dimension without this being limiting.

Ce mode de réalisation étant présenté à titre d'exemple non exhaustif. I1 revient à l'homme de métier de mettre en oeuvre les cultures de cellules formatrices d'os issues d'explants osseux et de biocristaux de test nacré de mollusque aquatique toutes les fois ou il aura besoin de produire artificiellement ou de l'os ou de la perle de culture. This embodiment being presented by way of non-exhaustive example. It is up to the person skilled in the art to implement the cultures of bone-forming cells derived from bone explants and of biocrystals of nacreous test of aquatic mollusk whenever it will need to produce artificially or bone or cultured pearl.

Claims (5)

REVENDICATIONS 1) Procédé de fabrication d'os ou de perle de culture caractérisé en ce qu'il est réalisé à partir des interactions histochimiques produites par la mise en culture de cellules formatrices d'os et de fragments ou de poudre de biocristaux issus de la couche nacré du test de mollusque aquatique.1) Method for manufacturing bone or cultured pearl, characterized in that it is produced from histochemical interactions produced by the cultivation of bone-forming cells and fragments or powder of biocrystals from the layer pearl of the aquatic mollusc test. 2) Procédé selon la revendication 1 caractérisé en ce que les fragments ou la poudre de biocristaux proviennent du test nacré de mollusques bivalves gastropodes ou céphalopodes.2) Method according to claim 1 characterized in that the fragments or the powder of biocrystals come from the pearly test of bivalve gastropod or cephalopod molluscs. 3) Procédé selon les revendications 1,2 caractérisé en ce que I'explant producteur de cellules formatrices d'os est issu d'os de mamifères. 3) Method according to claims 1,2 characterized in that the producer producing bone-forming cells comes from mammalian bones. 4) Procédé selon les revendications 1,2 et 3 caractérisé en ce que les cultures de cellules formatrices d'os et de biocristaux sont contenus dans des moules de formes et de dimensions variables destinés à en obtenir des répliques d'os ou de perle de culture.4) Method according to claims 1,2 and 3 characterized in that the cultures of bone-forming cells and biocrystals are contained in molds of variable shape and size intended to obtain replicas of bone or pearl of culture. 5) Utilisation de cellules formatrices d'os et de fragments ou de poudre de nacre pour la fabrication d'os artificiel animal ou humain.5) Use of bone-forming cells and fragments or pearl powder for the manufacture of artificial animal or human bone. sJ Ytilisation de cellules formatrices d'os et de fragments ou de poudre de nacre pour la fabrication de perle de culture.  sJ The use of bone-forming cells and fragments or pearl powder for the manufacture of cultured pearl.
FR9113083A 1991-10-23 1991-10-23 PRODUCTION OF BONE AND PEARL FROM CULTURE OF BONE FORMING CELLS. Withdrawn FR2682965A1 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
FR9113083A FR2682965A1 (en) 1991-10-23 1991-10-23 PRODUCTION OF BONE AND PEARL FROM CULTURE OF BONE FORMING CELLS.
PCT/FR1992/001008 WO1993008265A2 (en) 1991-10-23 1992-10-23 Production of bone and nacre from bone-forming cells in the presence of nacre
AU29487/92A AU2948792A (en) 1991-10-23 1992-10-26 Production of bone and nacre from bone-forming cells in the presence of nacre

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR9113083A FR2682965A1 (en) 1991-10-23 1991-10-23 PRODUCTION OF BONE AND PEARL FROM CULTURE OF BONE FORMING CELLS.

Publications (1)

Publication Number Publication Date
FR2682965A1 true FR2682965A1 (en) 1993-04-30

Family

ID=9418231

Family Applications (1)

Application Number Title Priority Date Filing Date
FR9113083A Withdrawn FR2682965A1 (en) 1991-10-23 1991-10-23 PRODUCTION OF BONE AND PEARL FROM CULTURE OF BONE FORMING CELLS.

Country Status (3)

Country Link
AU (1) AU2948792A (en)
FR (1) FR2682965A1 (en)
WO (1) WO1993008265A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5807554A (en) * 1997-04-11 1998-09-15 Yng-Wong; Quing Non Herbal formulations with nacre

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989002919A1 (en) * 1987-09-28 1989-04-06 Lee Dosuk Method for culturing pearls
DE3810803A1 (en) * 1988-03-30 1989-10-12 Battelle Institut E V Process for the production of a synthetic bone material with properties intrinsic to the body
JPH02142469A (en) * 1988-11-25 1990-05-31 Advance Co Ltd Zooblast culture in compound matrix composed of collagen and non-collagen protein
WO1990014111A1 (en) * 1989-05-23 1990-11-29 Serge Camprasse Prosthetic bones and dental roots made from mother of pearl

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4485096A (en) * 1982-02-26 1984-11-27 Massachusetts Institute Of Technology Tissue-equivalent and method for preparation thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989002919A1 (en) * 1987-09-28 1989-04-06 Lee Dosuk Method for culturing pearls
DE3810803A1 (en) * 1988-03-30 1989-10-12 Battelle Institut E V Process for the production of a synthetic bone material with properties intrinsic to the body
JPH02142469A (en) * 1988-11-25 1990-05-31 Advance Co Ltd Zooblast culture in compound matrix composed of collagen and non-collagen protein
WO1990014111A1 (en) * 1989-05-23 1990-11-29 Serge Camprasse Prosthetic bones and dental roots made from mother of pearl

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
WORLD PATENTS INDEX LATEST Week 9028, Derwent Publications Ltd., London, GB; AN 90-213058 & JP-A-2 142 469 (ADVANCE KK) 31 Mai 1990 *

Also Published As

Publication number Publication date
WO1993008265A3 (en) 1993-09-16
WO1993008265A2 (en) 1993-04-29
AU2948792A (en) 1993-05-21

Similar Documents

Publication Publication Date Title
Clarke et al. Designs from the deep: Marine organisms for bone tissue engineering
CA1152254A (en) Biodegradable implant used as bone prothesis
AU596508B2 (en) Skin substitute
DE69227103T2 (en) IMPLANTABLE TISSUE BASE AND METHOD FOR REDUCING IMMUNOGENITY
US5639654A (en) Process for creating a skin substitute and the resulting skin substitute
CN101020082B (en) Bone repairing material and its prepn process and use
WO1986002273A1 (en) Epidermalizing the surface of dermal-equivalent
CN1308434C (en) Device for active phase cell or tissue sampling and uses thereof
Ben-Nissan et al. Marine derived biomaterials for bone regeneration and tissue engineering: Learning from nature
KR101133644B1 (en) Method of preparing bioactive cellulose membrane from corneum of sea squirt, and Bioactive cellulose membrane prepared by the same
Viitala et al. Towards a synthetic osteo-odonto-keratoprosthesis
WO2003052084A2 (en) Cell constructs cultured in vitro, preparation and uses
Stricker et al. The ontogeny of shell secrtion in Terebratalia transversa (brachiopoda, articulata) II. Formation of the protegulum and juvenile shell
JPH06503735A (en) Wound dressing and its manufacturing method
WO2006000071A1 (en) Use of stem cells, method of tissue engineering, use of dental tissues and tooth biological substitute
FR2682965A1 (en) PRODUCTION OF BONE AND PEARL FROM CULTURE OF BONE FORMING CELLS.
RU2353397C2 (en) Bioabsorbable collagen matrix, method of production and application
CA2285161A1 (en) In vitro cultured ligament tissue and method of making same
EP0049341B1 (en) Process for conditioning alloplastic implantations and transplantations
EP1960011B1 (en) Use of a polysaccharide which is excreted by the vibrio diabolicus species for the engineering of non-mineralised connective tissue
Ersek et al. Processed bovine cartilage: an improved biosynthetic implant for contour defects
Ferranti et al. Biodegradable scaffolds based on plant stems for application in regenerative medicine
US20220112267A1 (en) Produce and isolate and/or extract collagen and/or gelatin from animal cell lines and/or tissue explants
Wilson Jr Biobased Materials for Medical Applications
DE19651992A1 (en) Skin cell culture medium comprising serum

Legal Events

Date Code Title Description
ST Notification of lapse