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ES2570633T3 - Método para detectar células - Google Patents

Método para detectar células

Info

Publication number
ES2570633T3
ES2570633T3 ES08792087T ES08792087T ES2570633T3 ES 2570633 T3 ES2570633 T3 ES 2570633T3 ES 08792087 T ES08792087 T ES 08792087T ES 08792087 T ES08792087 T ES 08792087T ES 2570633 T3 ES2570633 T3 ES 2570633T3
Authority
ES
Spain
Prior art keywords
wells
substance
coated
layer
main surface
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES08792087T
Other languages
English (en)
Inventor
Aishun Jin
Hiroyuki Kishi
Atsushi Muraguchi
Tsutomu Obata
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Valneva SE
Toyama Prefecture
Original Assignee
Valneva SE
Toyama Prefecture
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Valneva SE, Toyama Prefecture filed Critical Valneva SE
Application granted granted Critical
Publication of ES2570633T3 publication Critical patent/ES2570633T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5047—Cells of the immune system
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6863—Cytokines, i.e. immune system proteins modifying a biological response such as cell growth proliferation or differentiation, e.g. TNF, CNF, GM-CSF, lymphotoxin, MIF or their receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/52—Assays involving cytokines
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/715—Assays involving receptors, cell surface antigens or cell surface determinants for cytokines; for lymphokines; for interferons

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • General Engineering & Computer Science (AREA)
  • Sustainable Development (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Una matriz de micropocillos para detectar células diana que secretan una sustancia, que comprende múltiples pocillos en una de las superficies principales de un miembro de base, siendo los pocillos de un tamaño que permita la entrada de una única célula en cada pocillo, caracterizada por que una capa de una sustancia de unión capaz de unirse a al menos parte de una sustancia secretada por una célula diana que reviste la superficie principal alrededor de los pocillos, y no dentro de los pocillos, en donde la porción de la superficie principal que no está revestida por dicha capa está revestida con un agente bloqueante.
ES08792087T 2007-08-02 2008-08-01 Método para detectar células Active ES2570633T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007201493A JP4148367B1 (ja) 2007-08-02 2007-08-02 細胞のスクリーニング方法
PCT/JP2008/063874 WO2009017226A1 (ja) 2007-08-02 2008-08-01 細胞のスクリーニング方法

Publications (1)

Publication Number Publication Date
ES2570633T3 true ES2570633T3 (es) 2016-05-19

Family

ID=39787854

Family Applications (1)

Application Number Title Priority Date Filing Date
ES08792087T Active ES2570633T3 (es) 2007-08-02 2008-08-01 Método para detectar células

Country Status (9)

Country Link
US (2) US9494575B2 (es)
EP (1) EP2184345B1 (es)
JP (1) JP4148367B1 (es)
KR (1) KR101371840B1 (es)
CN (1) CN101861383B (es)
AU (1) AU2008283301B2 (es)
EA (2) EA201691174A1 (es)
ES (1) ES2570633T3 (es)
WO (1) WO2009017226A1 (es)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0923933A (ja) * 1995-07-13 1997-01-28 Roasu Kk パソコン用ラック等における補助テーブル
US7776553B2 (en) 2005-09-16 2010-08-17 Presidents And Fellows Of Harvard College Screening assays and methods
JP2010213649A (ja) * 2009-03-18 2010-09-30 Seiko Epson Corp 生体試料反応容器及び生体試料反応方法
US20130115606A1 (en) 2010-07-07 2013-05-09 The University Of British Columbia System and method for microfluidic cell culture
US10087408B2 (en) 2010-07-07 2018-10-02 The University Of British Columbia System and method for microfluidic cell culture
US9188593B2 (en) 2010-07-16 2015-11-17 The University Of British Columbia Methods for assaying cellular binding interactions
US9487583B2 (en) 2011-03-30 2016-11-08 National University Corporation University Of Toyama Method for selecting plasma cells or plasmablasts, method for producing target antigen specific antibodies, and novel monoclonal antibodies
WO2013000982A1 (en) 2011-06-27 2013-01-03 Vivalis Method for screening cells
JP6327734B2 (ja) * 2012-03-07 2018-05-23 国立大学法人富山大学 Ｔ細胞の刺激方法およびその利用
WO2014001459A1 (en) 2012-06-29 2014-01-03 Danmarks Tekniske Universitet A method of charging a test carrier and a test carrier
EP2708890B1 (en) * 2012-09-14 2015-11-18 Molecular Devices (New Milton) Ltd Method of selecting a monoclonal cell colony
JP6293409B2 (ja) * 2012-10-04 2018-03-14 国立大学法人富山大学 抗原特異的ウサギ抗体産生細胞の迅速な特定方法およびその利用
JP2014110785A (ja) * 2012-11-12 2014-06-19 Nagoya Univ 生理活性物質を分泌及び／又は表面提示する細胞の１細胞スクリーニング方法及びそれに用いられるイムノチャンバー
EP4079760A3 (en) * 2013-03-15 2023-01-25 Sanofi Pasteur Inc. Antibodies against clostridium difficile toxins and methods of using the same
EP3063543B1 (en) 2013-10-31 2020-03-11 Regeneron Pharmaceuticals, Inc. Competitive ligand binding assay for detecting neutralizing antibodies
WO2016149639A1 (en) 2015-03-19 2016-09-22 The Board Of Trustees Of The Leland Stanford Junior University Devices and methods for high-throughput single cell and biomolecule analysis and retrieval in a microfluidic chip
PL413909A1 (pl) 2015-09-15 2017-03-27 Wrocławskie Centrum Badań Eit + Spółka Z Ograniczoną Odpowiedzialnością Sposób detekcji i selekcji komórek hybrydomalnych produkujących pożądane przeciwciała
KR102713290B1 (ko) 2015-09-29 2024-10-04 도쿄 오카 고교 가부시키가이샤 기판, 구조체, 구조체의 제조 방법, 세포의 선별 방법, 세포의 제조 방법, 및 분비물의 생산 방법
EP3378488A4 (en) 2015-11-18 2019-10-30 Chugai Seiyaku Kabushiki Kaisha METHOD FOR IMPROVING THE HUMORAL IMMUNE REACTION
EA038880B1 (ru) 2016-01-11 2021-11-01 Форти Севен, Инк. Гуманизированные моноклональные антитела, моноклональные антитела мыши или химерные моноклональные антитела против cd47
WO2017170994A1 (ja) * 2016-03-31 2017-10-05 古河電気工業株式会社 細胞収容チップ
US11513076B2 (en) 2016-06-15 2022-11-29 Ludwig-Maximilians-Universität München Single molecule detection or quantification using DNA nanotechnology
EP3553158B1 (en) 2016-12-07 2024-11-20 Tokyo Ohka Kogyo Co., Ltd. Particle capture device
CN110799654A (zh) 2017-03-21 2020-02-14 穆韦尔斯有限公司 密封微孔测定法
US20230192845A1 (en) 2017-08-11 2023-06-22 Blink Biomedical Sas Cd96-binding agents as immunomodulators
JP7045924B2 (ja) 2018-05-11 2022-04-01 東京応化工業株式会社 細胞捕捉用フィルター膜及びその使用
DE102018214872A1 (de) * 2018-08-31 2020-03-05 Genome Identification Diagnostics Gmbh Verfahren zur in vitro Diagnose und/oder in vitro Therapieverfolgung einer Infektion und/oder Krankheit, insbesondere Infektionskrankheit, und/oder Tumorerkrankung und/oder Autoimmunerkrankung und/oder Allergie und/oder zur Impfkontrolle und/oder zum Nachweis einer Kreuzprotektivität sowie Verwendung eines Kits in einem solchen Verfahren
JP7019618B2 (ja) 2019-02-13 2022-02-15 古河電気工業株式会社 格納容器
CA3130051A1 (en) * 2019-02-18 2020-08-27 Nb Health Laboratory Co., Ltd. Method for selecting cells, method for producing nucleic acid, method for producing recombinant cells, method for producing target substance, method for producing pharmaceutical composition, and reagent
US11866685B2 (en) 2019-09-27 2024-01-09 University Of South Carolina Temperature responsive device for mechanobiological manipulation
KR20220070220A (ko) * 2019-09-30 2022-05-30 도오꾜오까고오교 가부시끼가이샤 분비물 산생 세포의 스크리닝 방법, 및 분비물 산생 세포의 스크리닝 키트
JP2021073888A (ja) * 2019-11-07 2021-05-20 国立大学法人富山大学 Ｔｃｒ様抗体の製造方法およびその利用
CN112626063B (zh) * 2020-12-18 2023-07-14 上海药明生物技术有限公司 一种利用cd138+生物标志物富集小鼠浆细胞制备杂交瘤的方法及应用
WO2022244852A1 (ja) 2021-05-19 2022-11-24 国立大学法人富山大学 癌の予防または治療のための医薬組成物
WO2024071374A1 (ja) * 2022-09-30 2024-04-04 富士フイルム株式会社 目的物質を産生する細胞のスクリーニング方法、核酸の製造方法、及び、目的物質の製造方法

Family Cites Families (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4529712A (en) * 1981-09-18 1985-07-16 Research Corporation Coated cells and their use
US5310674A (en) 1982-05-10 1994-05-10 Bar-Ilan University Apertured cell carrier
US5272081A (en) 1982-05-10 1993-12-21 Bar-Ilan University System and methods for cell selection
IL68507A (en) 1982-05-10 1986-01-31 Univ Bar Ilan System and methods for cell selection
EP0258565A3 (en) 1986-07-02 1989-05-24 E.I. Du Pont De Nemours And Company Location identifying means
JPH05240869A (ja) 1992-02-28 1993-09-21 Suzuki Motor Corp 血液等の凝集パターン出力装置
JPH0621509A (ja) 1992-07-06 1994-01-28 Fujitsu Ltd ポーラスシリコンの製造方法
US6410252B1 (en) 1995-12-22 2002-06-25 Case Western Reserve University Methods for measuring T cell cytokines
ATE347103T1 (de) 1996-09-06 2006-12-15 Ortho Mcneil Pharm Inc Reinigung von antigenspezifischen t-zellen
US6156389A (en) 1997-02-03 2000-12-05 Cytonix Corporation Hydrophobic coating compositions, articles coated with said compositions, and processes for manufacturing same
US6548263B1 (en) 1997-05-29 2003-04-15 Cellomics, Inc. Miniaturized cell array methods and apparatus for cell-based screening
US6232066B1 (en) 1997-12-19 2001-05-15 Neogen, Inc. High throughput assay system
WO1999039829A1 (en) 1998-02-04 1999-08-12 Merck & Co., Inc. Virtual wells for use in high throughput screening assays
US6210910B1 (en) 1998-03-02 2001-04-03 Trustees Of Tufts College Optical fiber biosensor array comprising cell populations confined to microcavities
US6087103A (en) 1998-03-04 2000-07-11 Lifespan Biosciences, Inc. Tagged ligand arrays for identifying target-ligand interactions
ATE227338T1 (de) 1998-03-18 2002-11-15 Massachusetts Inst Technology Vaskularisierte perfundierte anordnungen für mikrogewebe und mikroorgane
US20030027214A1 (en) 1999-02-17 2003-02-06 Kamb Carl Alexander Methods for substrate-ligand interaction screening
US7148058B2 (en) * 2000-06-05 2006-12-12 Chiron Corporation Protein microarrays on mirrored surfaces for performing proteomic analyses
DE10042947A1 (de) 2000-08-31 2002-03-21 Osram Opto Semiconductors Gmbh Strahlungsemittierendes Halbleiterbauelement auf GaN-Basis
US6734000B2 (en) 2000-10-12 2004-05-11 Regents Of The University Of California Nanoporous silicon support containing macropores for use as a bioreactor
US7264973B2 (en) * 2000-10-30 2007-09-04 Sru Biosystems, Inc. Label-free methods for performing assays using a colorimetric resonant optical biosensor
US7211209B2 (en) 2000-11-08 2007-05-01 Surface Logix, Inc. Method of making device for arraying biomolecules and for monitoring cell motility in real-time
EP1352952A1 (en) 2001-01-09 2003-10-15 Matsushita Electric Industrial Co., Ltd. Device for measuring extracellular potential, method of measuring extracellular potential by using the same and apparatus for quickly screening drug provided therewith
CN2478109Y (zh) * 2001-03-09 2002-02-20 赵翀 免疫检测板
US20020141906A1 (en) 2001-03-30 2002-10-03 American Type Culture Collection Microtiter plate sealing cover with well identifiers
JP4755368B2 (ja) 2001-07-24 2011-08-24 株式会社セルシード 高密度細胞アレイ用基板、製造法、及びその利用方法
US7169577B2 (en) 2001-07-27 2007-01-30 Surface Logix, Inc. Cell isolation and screening device and method of using same
JP4754746B2 (ja) 2001-09-28 2011-08-24 オリンパス株式会社 棒状担体およびこれを具備するシリンダー反応容器
US20050014201A1 (en) * 2001-10-25 2005-01-20 Mordechai Deuthsch Interactive transparent individual cells biochip processor
EP2093565B1 (en) 2002-05-22 2014-11-19 Platypus Technologies, Inc. Uses of devices and methods for cellular assays
WO2004008791A2 (de) 2002-07-10 2004-01-22 Siemens Aktiengesellschaft Übermittlung einer notruf-meldung an einen notrufempfänger
JP3723882B2 (ja) 2002-11-14 2005-12-07 篤 村口 抗原特異的リンパ球検出用マイクロウェルアレイチップ、抗原特異的リンパ球の検出法及び製造方法
US20060134704A1 (en) 2002-11-14 2006-06-22 Atsushi Muraguchi Microwell array chip for detecting antigen-specific lymphocytes, method of detecting and method of manufacturing antigen-specific lymphocytes, and method of cloning antigen-specific lymphocyte antigen receptor genes
JP3738308B2 (ja) 2002-11-29 2006-01-25 篤 村口 抗原特異的リンパ球抗原受容体遺伝子のクローニング方法
EP1614749A4 (en) 2003-03-28 2006-10-25 Toyama New Industry Organizaio METHOD OF CONSTRUCTING A HYBRIDOMA CELL PRODUCING AN ANTIGEN-SPECIFIC ANTIBODY USING A SINGLE ANTIGEN-SPECIFIC B-LYMPHOCYTE AND METHOD FOR THE PRODUCTION OF A MONOCLONAL ANTIBODY
WO2005025749A1 (en) 2003-09-08 2005-03-24 Irm, Llc Multi-well containers, systems, and methods of using the same
US20050220675A1 (en) 2003-09-19 2005-10-06 Reed Mark T High density plate filler
EP2381255A1 (en) 2003-09-25 2011-10-26 Toyama Prefecture Microwell array chip and its manufacturing method
JP2005253412A (ja) * 2004-03-15 2005-09-22 Masayasu Suzuki マイクロウェルアレイチップ、その製造方法及び被検体の活性検定方法
EP1977252B1 (en) * 2005-12-22 2012-12-05 Baxter International Inc. Improved monocyte activation test better able to detect non-endotoxin pyrogenic contaminants in medical products

Also Published As

Publication number Publication date
JP2009034047A (ja) 2009-02-19
AU2008283301A1 (en) 2009-02-05
JP4148367B1 (ja) 2008-09-10
HK1140789A1 (en) 2010-10-22
EA201691174A1 (ru) 2016-10-31
CN101861383B (zh) 2015-11-25
KR20100106294A (ko) 2010-10-01
US9494575B2 (en) 2016-11-15
EP2184345A1 (en) 2010-05-12
EA201070220A1 (ru) 2010-12-30
EP2184345A4 (en) 2010-10-27
CN101861383A (zh) 2010-10-13
EP2184345B1 (en) 2016-03-23
US9977017B2 (en) 2018-05-22
KR101371840B1 (ko) 2014-03-07
AU2008283301B2 (en) 2013-11-07
US20170023562A1 (en) 2017-01-26
US20110294678A1 (en) 2011-12-01
EA025219B1 (ru) 2016-12-30
WO2009017226A1 (ja) 2009-02-05

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