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ES2570591T3 - Detección de Staphylococcus aureus resistente a meticilina - Google Patents

Detección de Staphylococcus aureus resistente a meticilina

Info

Publication number
ES2570591T3
ES2570591T3 ES14179255T ES14179255T ES2570591T3 ES 2570591 T3 ES2570591 T3 ES 2570591T3 ES 14179255 T ES14179255 T ES 14179255T ES 14179255 T ES14179255 T ES 14179255T ES 2570591 T3 ES2570591 T3 ES 2570591T3
Authority
ES
Spain
Prior art keywords
methicillin
staphylococcus aureus
resistant staphylococcus
nucleic acids
aureus detection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES14179255T
Other languages
English (en)
Inventor
Irina A Afonina
Yevgeniy S Belousov
Walt Mahoney
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Elitechgroup Benelux BV
Original Assignee
Elitechgroup Benelux BV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Elitechgroup Benelux BV filed Critical Elitechgroup Benelux BV
Application granted granted Critical
Publication of ES2570591T3 publication Critical patent/ES2570591T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/156Polymorphic or mutational markers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/158Expression markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Un método para detectar un Staphylococcus aureus resistente a meticilina ("MRSA") en una muestra que contiene ácidos nucleicos que comprende: amplificar los ácidos nucleicos en la muestra; y detectar en la muestra ácidos nucleicos que comprenden los genes mecA, ldh1 y mecALGA251 amplificados, en el que la presencia de los genes mecA y ldh1 amplificados a una relación de aproximadamente 1:1 o los genes mecALGA251 y ldh1 a una relación de aproximadamente 1:1 indica la presencia de Staphylococcus aureus resistente a meticilina.
ES14179255T 2011-05-24 2012-05-24 Detección de Staphylococcus aureus resistente a meticilina Active ES2570591T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161489614P 2011-05-24 2011-05-24
US201161532454P 2011-09-08 2011-09-08
US201261614381P 2012-03-22 2012-03-22

Publications (1)

Publication Number Publication Date
ES2570591T3 true ES2570591T3 (es) 2016-05-19

Family

ID=47559639

Family Applications (2)

Application Number Title Priority Date Filing Date
ES14179255T Active ES2570591T3 (es) 2011-05-24 2012-05-24 Detección de Staphylococcus aureus resistente a meticilina
ES12815869.8T Active ES2537189T3 (es) 2011-05-24 2012-05-24 Detección de estafilococos áureos resistentes a la meticilina

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES12815869.8T Active ES2537189T3 (es) 2011-05-24 2012-05-24 Detección de estafilococos áureos resistentes a la meticilina

Country Status (7)

Country Link
US (2) US9677142B2 (es)
EP (2) EP2801626B1 (es)
JP (2) JP6196968B2 (es)
CA (1) CA2835283C (es)
DK (2) DK2801626T3 (es)
ES (2) ES2570591T3 (es)
WO (1) WO2013048583A2 (es)

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US10619219B2 (en) 2014-02-07 2020-04-14 University Of Iowa Research Foundation Oligonucleotide-based probes and methods for detection of microbes
US9920381B2 (en) * 2014-12-02 2018-03-20 Roche Molecular Systems, Inc. Compositions and methods for detecting MECC-containing methicillin-resistant Staphylococcus aureus
EP3230468B1 (en) 2014-12-12 2020-09-16 ELITechGroup, Inc. Methods and kits for detecting antibiotic resistant bacteria
WO2016140070A1 (ja) * 2015-03-03 2016-09-09 株式会社ダナフォーム 鋳型核酸の分析方法
JPWO2017038682A1 (ja) * 2015-08-28 2018-06-14 国立研究開発法人理化学研究所 鋳型核酸の分析方法、標的物質の分析方法、鋳型核酸または標的物質の分析用キット、および鋳型核酸または標的物質の分析用装置
US12378591B2 (en) 2017-09-29 2025-08-05 University Of Iowa Research Foundation Digital nuclease detection compositions and methods
CN111893169A (zh) * 2020-08-12 2020-11-06 济南国益生物科技有限公司 一种用于核酸扩增的冻干粉试剂及其制备方法和使用方法

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Also Published As

Publication number Publication date
EP2714939B1 (en) 2015-04-15
US9932643B2 (en) 2018-04-03
US20170183717A1 (en) 2017-06-29
CA2835283C (en) 2019-08-06
JP2014515268A (ja) 2014-06-30
JP6196968B2 (ja) 2017-09-13
WO2013048583A2 (en) 2013-04-04
EP2714939A2 (en) 2014-04-09
ES2537189T3 (es) 2015-06-03
DK2801626T3 (en) 2016-06-20
EP2801626A1 (en) 2014-11-12
US20150275274A1 (en) 2015-10-01
DK2714939T3 (en) 2015-06-29
CA2835283A1 (en) 2013-04-04
EP2801626B1 (en) 2016-04-06
JP2017158576A (ja) 2017-09-14
WO2013048583A3 (en) 2013-08-29
US9677142B2 (en) 2017-06-13

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