ES2570591T3 - Detección de Staphylococcus aureus resistente a meticilina - Google Patents
Detección de Staphylococcus aureus resistente a meticilinaInfo
- Publication number
- ES2570591T3 ES2570591T3 ES14179255T ES14179255T ES2570591T3 ES 2570591 T3 ES2570591 T3 ES 2570591T3 ES 14179255 T ES14179255 T ES 14179255T ES 14179255 T ES14179255 T ES 14179255T ES 2570591 T3 ES2570591 T3 ES 2570591T3
- Authority
- ES
- Spain
- Prior art keywords
- methicillin
- staphylococcus aureus
- resistant staphylococcus
- nucleic acids
- aureus detection
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- RJQXTJLFIWVMTO-TYNCELHUSA-N Methicillin Chemical compound COC1=CC=CC(OC)=C1C(=O)N[C@@H]1C(=O)N2[C@@H](C(O)=O)C(C)(C)S[C@@H]21 RJQXTJLFIWVMTO-TYNCELHUSA-N 0.000 title abstract 3
- 241000191967 Staphylococcus aureus Species 0.000 title abstract 3
- 229960003085 meticillin Drugs 0.000 title abstract 3
- 238000001514 detection method Methods 0.000 title 1
- 101150026107 ldh1 gene Proteins 0.000 abstract 3
- 108020004707 nucleic acids Proteins 0.000 abstract 3
- 150000007523 nucleic acids Chemical class 0.000 abstract 3
- 102000039446 nucleic acids Human genes 0.000 abstract 3
- 206010041925 Staphylococcal infections Diseases 0.000 abstract 1
- 208000015688 methicillin-resistant staphylococcus aureus infectious disease Diseases 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Un método para detectar un Staphylococcus aureus resistente a meticilina ("MRSA") en una muestra que contiene ácidos nucleicos que comprende: amplificar los ácidos nucleicos en la muestra; y detectar en la muestra ácidos nucleicos que comprenden los genes mecA, ldh1 y mecALGA251 amplificados, en el que la presencia de los genes mecA y ldh1 amplificados a una relación de aproximadamente 1:1 o los genes mecALGA251 y ldh1 a una relación de aproximadamente 1:1 indica la presencia de Staphylococcus aureus resistente a meticilina.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161489614P | 2011-05-24 | 2011-05-24 | |
| US201161532454P | 2011-09-08 | 2011-09-08 | |
| US201261614381P | 2012-03-22 | 2012-03-22 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2570591T3 true ES2570591T3 (es) | 2016-05-19 |
Family
ID=47559639
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES14179255T Active ES2570591T3 (es) | 2011-05-24 | 2012-05-24 | Detección de Staphylococcus aureus resistente a meticilina |
| ES12815869.8T Active ES2537189T3 (es) | 2011-05-24 | 2012-05-24 | Detección de estafilococos áureos resistentes a la meticilina |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES12815869.8T Active ES2537189T3 (es) | 2011-05-24 | 2012-05-24 | Detección de estafilococos áureos resistentes a la meticilina |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US9677142B2 (es) |
| EP (2) | EP2801626B1 (es) |
| JP (2) | JP6196968B2 (es) |
| CA (1) | CA2835283C (es) |
| DK (2) | DK2801626T3 (es) |
| ES (2) | ES2570591T3 (es) |
| WO (1) | WO2013048583A2 (es) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013093106A1 (en) * | 2011-12-23 | 2013-06-27 | Biomerieux | Detection of meca variant strains of methicillin-resistant staphylococcus aureus |
| US10407739B2 (en) | 2013-09-23 | 2019-09-10 | Quest Diagnostics Investments Incorporated | Detection of methicillin-resistant Staphylococcus aureus in biological samples |
| US10619219B2 (en) | 2014-02-07 | 2020-04-14 | University Of Iowa Research Foundation | Oligonucleotide-based probes and methods for detection of microbes |
| US9920381B2 (en) * | 2014-12-02 | 2018-03-20 | Roche Molecular Systems, Inc. | Compositions and methods for detecting MECC-containing methicillin-resistant Staphylococcus aureus |
| EP3230468B1 (en) | 2014-12-12 | 2020-09-16 | ELITechGroup, Inc. | Methods and kits for detecting antibiotic resistant bacteria |
| WO2016140070A1 (ja) * | 2015-03-03 | 2016-09-09 | 株式会社ダナフォーム | 鋳型核酸の分析方法 |
| JPWO2017038682A1 (ja) * | 2015-08-28 | 2018-06-14 | 国立研究開発法人理化学研究所 | 鋳型核酸の分析方法、標的物質の分析方法、鋳型核酸または標的物質の分析用キット、および鋳型核酸または標的物質の分析用装置 |
| US12378591B2 (en) | 2017-09-29 | 2025-08-05 | University Of Iowa Research Foundation | Digital nuclease detection compositions and methods |
| CN111893169A (zh) * | 2020-08-12 | 2020-11-06 | 济南国益生物科技有限公司 | 一种用于核酸扩增的冻干粉试剂及其制备方法和使用方法 |
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-
2012
- 2012-05-24 ES ES14179255T patent/ES2570591T3/es active Active
- 2012-05-24 EP EP14179255.6A patent/EP2801626B1/en active Active
- 2012-05-24 ES ES12815869.8T patent/ES2537189T3/es active Active
- 2012-05-24 CA CA2835283A patent/CA2835283C/en active Active
- 2012-05-24 US US13/479,557 patent/US9677142B2/en active Active
- 2012-05-24 JP JP2014512092A patent/JP6196968B2/ja not_active Expired - Fee Related
- 2012-05-24 DK DK14179255.6T patent/DK2801626T3/en active
- 2012-05-24 WO PCT/US2012/039275 patent/WO2013048583A2/en not_active Ceased
- 2012-05-24 EP EP12815869.8A patent/EP2714939B1/en active Active
- 2012-05-24 DK DK12815869.8T patent/DK2714939T3/en active
-
2017
- 2017-03-09 US US15/454,794 patent/US9932643B2/en active Active
- 2017-05-08 JP JP2017092369A patent/JP2017158576A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP2714939B1 (en) | 2015-04-15 |
| US9932643B2 (en) | 2018-04-03 |
| US20170183717A1 (en) | 2017-06-29 |
| CA2835283C (en) | 2019-08-06 |
| JP2014515268A (ja) | 2014-06-30 |
| JP6196968B2 (ja) | 2017-09-13 |
| WO2013048583A2 (en) | 2013-04-04 |
| EP2714939A2 (en) | 2014-04-09 |
| ES2537189T3 (es) | 2015-06-03 |
| DK2801626T3 (en) | 2016-06-20 |
| EP2801626A1 (en) | 2014-11-12 |
| US20150275274A1 (en) | 2015-10-01 |
| DK2714939T3 (en) | 2015-06-29 |
| CA2835283A1 (en) | 2013-04-04 |
| EP2801626B1 (en) | 2016-04-06 |
| JP2017158576A (ja) | 2017-09-14 |
| WO2013048583A3 (en) | 2013-08-29 |
| US9677142B2 (en) | 2017-06-13 |
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