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ES2126576T3 - Composiciones y procedimientos con luciferasa - Google Patents

Composiciones y procedimientos con luciferasa

Info

Publication number
ES2126576T3
ES2126576T3 ES91919421T ES91919421T ES2126576T3 ES 2126576 T3 ES2126576 T3 ES 2126576T3 ES 91919421 T ES91919421 T ES 91919421T ES 91919421 T ES91919421 T ES 91919421T ES 2126576 T3 ES2126576 T3 ES 2126576T3
Authority
ES
Spain
Prior art keywords
luciferasa
coa
test
beetle
luciferin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES91919421T
Other languages
English (en)
Other versions
ES2126576T5 (es
Inventor
Keith V Wood
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Promega Corp
Original Assignee
Promega Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=24320802&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2126576(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Promega Corp filed Critical Promega Corp
Application granted granted Critical
Publication of ES2126576T3 publication Critical patent/ES2126576T3/es
Publication of ES2126576T5 publication Critical patent/ES2126576T5/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0069Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y113/00Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/12Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
    • C12Y113/12007Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) (1.13.12.7), i.e. firefly-luciferase
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00Chemistry: molecular biology and microbiology
    • Y10S435/81Packaged device or kit
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00Chemistry: molecular biology and microbiology
    • Y10S435/968High energy substrates, e.g. fluorescent, chemiluminescent, radioactive
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00Chemistry: molecular biology and microbiology
    • Y10S435/975Kit
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00Chemistry: analytical and immunological testing
    • Y10S436/807Apparatus included in process claim, e.g. physical support structures
    • Y10S436/808Automated or kit

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

SE DESCRIBEN METODOS Y COMPOSICIONES PARA PRODUCCION LIGERA DE CINETICA MEJORADA DE ACTIVIDAD LUCIFERASA EN REACCIONES DEL ESCARABAJO LUCIFERASA-LUCIFERIN. DE AQUI, LA INVENCION PROVEE, METODOS, COMPOSICIONES Y KITS DE PRUEBA PARA MUESTRAS DE INVESTIGACION PARA LA PRESENCIA DE ESCARABAJO LUCIFERASA O, UTILIZANDO REACCION DE ESCARABAJO LUCIFERASA-LUCIFERIN, PARA LA DETERMINACION DE LA PRESENCIA DE ATP. LA INVENCION SE REFIERE TAMBIEN AL COMPUESTO COMPLEJO DE COENZIMA, COENZIMA A, UN ESCARABAJO LUCIFERASA Y OXILUCIFERIN EN SU ESTADO DE SALIDA EN LA REACCION DE LUCIFERASA-LUCIFERIN Y EL LUCIFERIL-COA, EL TIOESTER DE COENZIMA A Y LUCIFERIN (D ENZOTIAZOLIL)-TIAZOLINA PRESENTA LAS CURVAS DE LUMINISCENCIA EN DONDE SE HACE PRESENTE UNA COMBINACION DE COA, DTT Y BSA EN EL ENSAYO REALIZADO, ESTANDO PRESENTE SOLAMENTE COA EN EL ENSAYO, Y DONDE NO EXISTEN ADICIONES AL ENSAYO O PRUEBA CONVENCIONAL. EL EFECTO COMBINADO DE COA, DTT Y BSA RESULTA EN UNA PRODUCCION TOTAL DE LUZ MAYOR QUE 15 VECES EN LA SITUACION CON NO ADICIONES.
ES91919421T 1990-09-10 1991-09-09 Composiciones y procedimientos con luciferasa. Expired - Lifetime ES2126576T5 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/580,371 US5283179A (en) 1990-09-10 1990-09-10 Luciferase assay method
US580371 2009-10-16

Publications (2)

Publication Number Publication Date
ES2126576T3 true ES2126576T3 (es) 1999-04-01
ES2126576T5 ES2126576T5 (es) 2005-12-01

Family

ID=24320802

Family Applications (1)

Application Number Title Priority Date Filing Date
ES91919421T Expired - Lifetime ES2126576T5 (es) 1990-09-10 1991-09-09 Composiciones y procedimientos con luciferasa.

Country Status (10)

Country Link
US (4) US5283179A (es)
EP (1) EP0553234B2 (es)
JP (2) JP3171595B2 (es)
AT (1) ATE173298T1 (es)
AU (1) AU649289B2 (es)
DE (1) DE69130482T3 (es)
DK (1) DK0553234T4 (es)
ES (1) ES2126576T5 (es)
GR (1) GR3029352T3 (es)
WO (1) WO1992004468A1 (es)

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JPS6163855A (ja) * 1984-09-05 1986-04-02 Fuji Photo Film Co Ltd 静電写真用液体現像剤
DK258787D0 (da) * 1987-05-21 1987-05-21 Foss Electric As N Fremgangsmaade til bestemmelse af bakteriekoncentration i en proeve
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JP2001224398A (ja) 2001-08-21
US5650289A (en) 1997-07-22
DE69130482T2 (de) 1999-05-27
EP0553234A1 (en) 1993-08-04
EP0553234A4 (es) 1995-08-02
ES2126576T5 (es) 2005-12-01
US5283179A (en) 1994-02-01
EP0553234B1 (en) 1998-11-11
JP3171595B2 (ja) 2001-05-28
DK0553234T4 (da) 2005-08-01
DK0553234T3 (da) 1999-07-26
DE69130482T3 (de) 2006-05-04
EP0553234B2 (en) 2005-07-13
ATE173298T1 (de) 1998-11-15
AU8858391A (en) 1992-03-30
GR3029352T3 (en) 1999-05-28
JPH06500921A (ja) 1994-01-27
WO1992004468A1 (en) 1992-03-19
AU649289B2 (en) 1994-05-19
US5814471A (en) 1998-09-29
US5641641A (en) 1997-06-24
DE69130482D1 (de) 1998-12-17

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