[go: up one dir, main page]

ES2192945B1 - Un metodo para interferir con la infeccion de virus en plantas. - Google Patents

Un metodo para interferir con la infeccion de virus en plantas.

Info

Publication number
ES2192945B1
ES2192945B1 ES200101593A ES200101593A ES2192945B1 ES 2192945 B1 ES2192945 B1 ES 2192945B1 ES 200101593 A ES200101593 A ES 200101593A ES 200101593 A ES200101593 A ES 200101593A ES 2192945 B1 ES2192945 B1 ES 2192945B1
Authority
ES
Spain
Prior art keywords
whose
plant
plants
virus infection
ssrna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES200101593A
Other languages
English (en)
Other versions
ES2192945A1 (es
Inventor
Francisco Tenllado Peralo
Jose Ramon Diaz Ruiz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Consejo Superior de Investigaciones Cientificas CSIC
Original Assignee
Consejo Superior de Investigaciones Cientificas CSIC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Consejo Superior de Investigaciones Cientificas CSIC filed Critical Consejo Superior de Investigaciones Cientificas CSIC
Priority to ES200101593A priority Critical patent/ES2192945B1/es
Priority to EP02743278A priority patent/EP1416049A1/en
Priority to PCT/ES2002/000319 priority patent/WO2003004649A1/es
Publication of ES2192945A1 publication Critical patent/ES2192945A1/es
Application granted granted Critical
Publication of ES2192945B1 publication Critical patent/ES2192945B1/es
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

Un método para interferir con la infección de virus en plantas. Se revela un método para interferir o degradar de forma especifica (preferentemente mediante silenciamiento génico) cualquier secuencia nucleotídica de RNA de cadena sencilla (ssRNA) (preferentemente RNA mensajeros de virus, de genes endógenos o de transgenes) en las células de cualquier planta, cuyo método consiste en la aplicación directa en cualquier tejido de la planta (preferentemente hojas) y por cualquier método de aplicación (preferentemente inoculación o infiltración) de moléculas de RNA de doble cadena (dsRNA) producidas por cualquier procedimiento natural o sintético (preferentemente mediante transcripción in vitro) cuyas moléculas de dsRNA tienen suficiente similitud de secuencia con cualquier fragmento del ssRNA que se quiere degradar, o cuya expresión se quiere silenciar, en la planta.
ES200101593A 2001-07-06 2001-07-06 Un metodo para interferir con la infeccion de virus en plantas. Expired - Fee Related ES2192945B1 (es)

Priority Applications (3)

Application Number Priority Date Filing Date Title
ES200101593A ES2192945B1 (es) 2001-07-06 2001-07-06 Un metodo para interferir con la infeccion de virus en plantas.
EP02743278A EP1416049A1 (en) 2001-07-06 2002-06-28 Method of interfering with a virus infection in plants
PCT/ES2002/000319 WO2003004649A1 (es) 2001-07-06 2002-06-28 Un método para interferir con la infección de virus en plantas

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES200101593A ES2192945B1 (es) 2001-07-06 2001-07-06 Un metodo para interferir con la infeccion de virus en plantas.

Publications (2)

Publication Number Publication Date
ES2192945A1 ES2192945A1 (es) 2003-10-16
ES2192945B1 true ES2192945B1 (es) 2005-03-01

Family

ID=8498334

Family Applications (1)

Application Number Title Priority Date Filing Date
ES200101593A Expired - Fee Related ES2192945B1 (es) 2001-07-06 2001-07-06 Un metodo para interferir con la infeccion de virus en plantas.

Country Status (3)

Country Link
EP (1) EP1416049A1 (es)
ES (1) ES2192945B1 (es)
WO (1) WO2003004649A1 (es)

Families Citing this family (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4505626B2 (ja) * 2003-12-15 2010-07-21 独立行政法人農業生物資源研究所 花粉特異的発現活性を有するプロモーター
JP4505627B2 (ja) * 2003-12-15 2010-07-21 独立行政法人農業生物資源研究所 緑色組織特異的発現活性を有するプロモーター
JP4505628B2 (ja) * 2003-12-15 2010-07-21 独立行政法人農業生物資源研究所 葉特異的発現活性を有するプロモーター
ES2444001T3 (es) * 2004-10-21 2014-02-21 Venganza Inc. Procedimientos y materiales para conferir resistencia a plagas y patógenos de plantas
US8097712B2 (en) * 2007-11-07 2012-01-17 Beelogics Inc. Compositions for conferring tolerance to viral disease in social insects, and the use thereof
US8822426B2 (en) 2009-05-05 2014-09-02 Beeologics Inc. Prevention and treatment of nosema disease in bees
CN102481311A (zh) 2009-08-21 2012-05-30 比欧洛吉科斯公司 经由植物转录的分子预防和治疗有益昆虫的疾病
US8962584B2 (en) 2009-10-14 2015-02-24 Yissum Research Development Company Of The Hebrew University Of Jerusalem, Ltd. Compositions for controlling Varroa mites in bees
DK3231872T3 (da) * 2010-03-08 2020-07-20 Monsanto Technology Llc Polynukleotidmolekyler til genregulering i planter
US10760086B2 (en) 2011-09-13 2020-09-01 Monsanto Technology Llc Methods and compositions for weed control
BR112014005958A2 (pt) 2011-09-13 2020-10-13 Monsanto Technology Llc métodos e composições químicas agrícolas para controle de planta, método de redução de expressão de um gene accase em uma planta, cassete de expressão microbiana, método para fazer um polinucleotídeo, método de identificação de polinucleotídeos úteis na modulação de expressão do gene accase e composição herbicida
US9840715B1 (en) 2011-09-13 2017-12-12 Monsanto Technology Llc Methods and compositions for delaying senescence and improving disease tolerance and yield in plants
WO2013040049A1 (en) 2011-09-13 2013-03-21 Monsanto Technology Llc Methods and compositions for weed control
US10806146B2 (en) 2011-09-13 2020-10-20 Monsanto Technology Llc Methods and compositions for weed control
US10829828B2 (en) 2011-09-13 2020-11-10 Monsanto Technology Llc Methods and compositions for weed control
CA2848695A1 (en) 2011-09-13 2013-03-21 Monsanto Technology Llc Methods and composition for weed control comprising inhibiting ppg oxidase
MX350771B (es) 2011-09-13 2017-09-15 Monsanto Technology Llc Métodos y composiciones para el control de malezas.
US9920326B1 (en) 2011-09-14 2018-03-20 Monsanto Technology Llc Methods and compositions for increasing invertase activity in plants
IN2014MN02404A (es) 2012-05-24 2015-08-21 Seeds Ltd Ab
WO2014062989A2 (en) 2012-10-18 2014-04-24 Monsanto Technology Llc Methods and compositions for plant pest control
US10683505B2 (en) 2013-01-01 2020-06-16 Monsanto Technology Llc Methods of introducing dsRNA to plant seeds for modulating gene expression
UY35251A (es) * 2013-01-01 2014-07-31 Seeds Ltd Ab MOLÉCULAS DE dsRNA AISLADAS Y MÉTODOS PARA USARLAS PARA SILENCIAR MOLÉCULAS DIANA DE INTERÉS
US10000767B2 (en) 2013-01-28 2018-06-19 Monsanto Technology Llc Methods and compositions for plant pest control
MX2015012334A (es) 2013-03-13 2016-02-05 Monsanto Technology Llc Metodos y composiciones para el control de malezas.
CA2905027A1 (en) 2013-03-13 2014-10-09 Monsanto Technology Llc Methods and compositions for weed control
US20140283211A1 (en) 2013-03-14 2014-09-18 Monsanto Technology Llc Methods and Compositions for Plant Pest Control
US10568328B2 (en) 2013-03-15 2020-02-25 Monsanto Technology Llc Methods and compositions for weed control
JP6668236B2 (ja) 2013-07-19 2020-03-18 モンサント テクノロジー エルエルシー Ｌｅｐｔｉｎｏｔａｒｓａ防除用組成物及びその方法
US9850496B2 (en) 2013-07-19 2017-12-26 Monsanto Technology Llc Compositions and methods for controlling Leptinotarsa
US9540642B2 (en) 2013-11-04 2017-01-10 The United States Of America, As Represented By The Secretary Of Agriculture Compositions and methods for controlling arthropod parasite and pest infestations
UA119253C2 (uk) 2013-12-10 2019-05-27 Біолоджикс, Інк. Спосіб боротьби із вірусом у кліща varroa та у бджіл
BR112016014487B1 (pt) 2013-12-20 2021-06-22 The University Of Queensland Composição de rna de interferência de proteção de planta compreendendo rna de fita dupla de proteção de planta adsorvido sobre partículas de hidróxido duplo lamelar, seu processo de preparação, e processo para proteção de uma planta
CN105979770B (zh) 2014-01-15 2019-07-05 孟山都技术公司 用于使用epsps多核苷酸的杂草控制的方法和组合物
CN106413390B (zh) 2014-04-01 2019-09-27 孟山都技术公司 用于控制虫害的组合物和方法
EP3158067B1 (en) 2014-06-23 2020-08-12 Monsanto Technology LLC Compositions and methods for regulating gene expression via rna interference
US11807857B2 (en) 2014-06-25 2023-11-07 Monsanto Technology Llc Methods and compositions for delivering nucleic acids to plant cells and regulating gene expression
AR101348A1 (es) 2014-07-29 2016-12-14 Monsanto Technology Llc Composiciones y métodos para el control de pestes por insectos
US10968449B2 (en) 2015-01-22 2021-04-06 Monsanto Technology Llc Compositions and methods for controlling Leptinotarsa
CN107750125A (zh) 2015-06-02 2018-03-02 孟山都技术有限公司 用于将多核苷酸递送至植物中的组合物和方法
AU2016270913A1 (en) 2015-06-03 2018-01-04 Monsanto Technology Llc Methods and compositions for introducing nucleic acids into plants
MX370604B (es) 2015-06-19 2019-12-18 Univ Queensland Composicion.
WO2019051232A1 (en) 2017-09-11 2019-03-14 Altria Client Services Llc COMPOSITIONS AND METHODS FOR PRODUCING TOBACCO PLANTS AND PRODUCTS HAVING REDUCED OR ELIMINATED GOURMETS

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999032619A1 (en) * 1997-12-23 1999-07-01 The Carnegie Institution Of Washington Genetic inhibition by double-stranded rna
WO1999061631A1 (en) * 1998-05-26 1999-12-02 Novartis Ag Dsrna-mediated regulation of gene expression in plants
WO2000044914A1 (en) * 1999-01-28 2000-08-03 Medical College Of Georgia Research Institute, Inc. Composition and method for in vivo and in vitro attenuation of gene expression using double stranded rna

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001037654A2 (en) * 1999-11-24 2001-05-31 Dna Plant Technology Corporation METHOD OF EXPRESSING dsRNA IN PLANTS TO INHIBIT INSECT PESTS

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999032619A1 (en) * 1997-12-23 1999-07-01 The Carnegie Institution Of Washington Genetic inhibition by double-stranded rna
WO1999061631A1 (en) * 1998-05-26 1999-12-02 Novartis Ag Dsrna-mediated regulation of gene expression in plants
WO2000044914A1 (en) * 1999-01-28 2000-08-03 Medical College Of Georgia Research Institute, Inc. Composition and method for in vivo and in vitro attenuation of gene expression using double stranded rna

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
WATERHOUSE et al. "Virus resistance and gene silencing in plants can be induced by simultaneous expression of sense and antisense RNA" Proceedings of the National Academy of USA, (1998), Vol. 95, páginas 13959-13964. *

Also Published As

Publication number Publication date
ES2192945A1 (es) 2003-10-16
WO2003004649A1 (es) 2003-01-16
EP1416049A1 (en) 2004-05-06

Similar Documents

Publication Publication Date Title
ES2192945B1 (es) Un metodo para interferir con la infeccion de virus en plantas.
Gatignol et al. Dual role of TRBP in HIV replication and RNA interference: viral diversion of a cellular pathway or evasion from antiviral immunity?
PA8813901A1 (es) Paramixovirus oncolíticos atenuados que codifican citoquinas de aves
WO2020198509A3 (en) Modified oligonucleotides with increased stability
ATE526407T1 (de) Insektenresistenz durch inhibierung von genexpression
EA201200217A1 (ru) Рнк с комбинацией из немодифицированных и модифицированных нуклеотидов для экспрессии белков
BR0109530A (pt) Promotores de vìrus de encrespamento de folha amarela de cestrum
CY1119062T1 (el) Μικρα rna μορια που προκαλουν παρεμβαση rna
BRPI0516874A (pt) micrornas
AU2003261231A8 (en) Modified small interfering rna molecules and methods of use
HUP0103556A2 (hu) Kukorica cellulóz szintázok és alkalmazásaik
HUP0102103A2 (hu) A génexpresszió szabályozása növényekben dsRNS segítségével
AR039717A1 (es) Construcciones de arn de intrones cadena doble y usos de las mismas
Sun et al. Mitochondrial DNA damage and oxidative damage in HL-60 cells exposed to 900 MHz radiofrequency fields
MX348226B (es) Uso de v1p3ab en combinacion con cry1ca para el manejo de insectos resistentes.
MX2025006880A (es) Metodos para generar celulas madre hematopoyeticas
Mills et al. Inducing RNA interference in the arbovirus vector, C ulicoides sonorensis
AR079813A1 (es) Metodos para controlar el nematodo de las lesiones de las raices
MX2020010802A (es) Moleculas inhibidoras de acidos nucleicos de caracter bicatenario modificadas con nucleotidos que aumentan la tm.
CL2021002842A1 (es) Moléculas inhibidoras de ácido nucleico bicatenario con cadenas de sentido acortadas
RU2016131028A (ru) Композиции, содержащие асимметричную интерферирующую рнк, которые подвергают сайленсингу k-ras, и способы их применения
WO2004022782A3 (en) Compositions and methods for tissue specific or inducible inhibition of gene expression
DE60124526D1 (de) Methode zur identifizierung von helicobacter antigenen
ATE356204T1 (de) Antisense-zusammensetzungen und verfahren zur behandlung von krebs
Kang et al. Optimization of double‐stranded RNAi intrathoracic injection method in Aedes aegypti

Legal Events

Date Code Title Description
EC2A Search report published

Date of ref document: 20031016

Kind code of ref document: A1

FG2A Definitive protection

Ref document number: 2192945B1

Country of ref document: ES

FD2A Announcement of lapse in spain

Effective date: 20180807