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ES2181775T3 - Nuevos complejos de acido nucleico y de polimero, su procedimiento de preparacion y su utilizacion para la transfeccion de celulas. - Google Patents

Nuevos complejos de acido nucleico y de polimero, su procedimiento de preparacion y su utilizacion para la transfeccion de celulas.

Info

Publication number
ES2181775T3
ES2181775T3 ES95918049T ES95918049T ES2181775T3 ES 2181775 T3 ES2181775 T3 ES 2181775T3 ES 95918049 T ES95918049 T ES 95918049T ES 95918049 T ES95918049 T ES 95918049T ES 2181775 T3 ES2181775 T3 ES 2181775T3
Authority
ES
Spain
Prior art keywords
nucleic acid
sup
sub
functions
free
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES95918049T
Other languages
English (en)
Inventor
Patrick Midoux
Patrick Erbacher
Annie-Claude Roche-Degremont
Michel Monsigny
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
IDM Immuno Designed Molecules
Original Assignee
IDM Immuno Designed Molecules
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by IDM Immuno Designed Molecules filed Critical IDM Immuno Designed Molecules
Application granted granted Critical
Publication of ES2181775T3 publication Critical patent/ES2181775T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/62—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being a protein, peptide or polyamino acid
    • A61K47/64—Drug-peptide, drug-protein or drug-polyamino acid conjugates, i.e. the modifying agent being a peptide, protein or polyamino acid which is covalently bonded or complexed to a therapeutically active agent
    • A61K47/645—Polycationic or polyanionic oligopeptides, polypeptides or polyamino acids, e.g. polylysine, polyarginine, polyglutamic acid or peptide TAT

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Public Health (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Microbiology (AREA)
  • Veterinary Medicine (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
  • Medicinal Preparation (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

LA INVENCION SE REFIERE A UN COMPLEJO ENTRE AL MENOS UN ACIDO NUCLEICO CARGADO NEGATIVAMENTE Y AL MENOS UN CONJUGADO POLIMERICO CARGADO POSITIVAMENTE, SIENDO LA CONEXION ENTRE EL ACIDO NUCLEICO Y EL CONJUGADO POLIMERICO DE NATURALEZA ELECTROSTATICA, CONTENIENDO EL CONJUGADO POLIMERICO UN POLIMERO FORMADO DE UNIDADES MOMOMERAS QUE LLEVAN FUNCIONES NH{SUB,3}{SUP,+} LIBRES DE DICHAS UNIDADES Y SIENDO TALES QUE: LAS FUNCIONES NH{SUB,3}{SUP,+} LIBRES DE DICHAS UNIDADES ESTAN SUSTITUIDAS EN UNA RELACION DE AL MENOS 10%, VENTAJOSAMENTE DE 45% A 70%, PARTICULARMENTE DE 60%, POR RESIDUOS NO CARGADOS QUE CONLLEVAN UNA REDUCCION DE LAS CARGAS POSITIVAS RESPECTO AL MISMO CONJUGADO POLIMERICO NO SUSTITUIDO, FACILITANDO LA LIBERACION DEL ACIDO NUCLEICO POR LA DISOCIACION DEL COMPLEJO, POSEYENDO DICHOS RESIDUOS ADEMAS LAS SIGUIENTES PROPIEDADES: LLEVAN AL MENOS UN GRUPO HIDROXILO, NO CORRESPONDEN A NINGUNA SEÑAL DE RECONOCIMIENTO RECONOCIDA POR UN RECEPTOR DE MEMBRANA CELULAR, PUDIENDO LAS FUNCIONES NH{SUB,3}{SUP,+} LIBRES DE DICHAS UNIDADES Y/O LOS GRUPOS HIDROXILOS DE DICHOS RESIDUOS TAMBIEN SER SUSTITUIDAS POR AL MENOS UNA MOLECULAS QUE CONSTITUYE UNA SEÑAL DE RECONOCIMIENTO RECONOCIDA POR UN RECEPTOR DE MEMBRANA CELULAR, SIEMPRE QUE EL CONJUGADO POLIMERICO CONTENGA AL MENOS 30% DE FUNCIONES NH{SUB,3}{SUP,+} LIBRES.
ES95918049T 1994-04-28 1995-04-24 Nuevos complejos de acido nucleico y de polimero, su procedimiento de preparacion y su utilizacion para la transfeccion de celulas. Expired - Lifetime ES2181775T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR9405174A FR2719316B1 (fr) 1994-04-28 1994-04-28 Nouveaux complexes d'acide nucléique et de polymère, leur procédé de préparation et leur utilisation pour la transfection de cellules.

Publications (1)

Publication Number Publication Date
ES2181775T3 true ES2181775T3 (es) 2003-03-01

Family

ID=9462633

Family Applications (1)

Application Number Title Priority Date Filing Date
ES95918049T Expired - Lifetime ES2181775T3 (es) 1994-04-28 1995-04-24 Nuevos complejos de acido nucleico y de polimero, su procedimiento de preparacion y su utilizacion para la transfeccion de celulas.

Country Status (9)

Country Link
US (1) US5595897A (es)
EP (1) EP0753070B1 (es)
AT (1) ATE224957T1 (es)
AU (1) AU695056B2 (es)
CA (1) CA2187629C (es)
DE (1) DE69528363T2 (es)
ES (1) ES2181775T3 (es)
FR (1) FR2719316B1 (es)
WO (1) WO1995030020A1 (es)

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US5674908A (en) * 1993-12-20 1997-10-07 Life Technologies, Inc. Highly packed polycationic ammonium, sulfonium and phosphonium lipids
US6989434B1 (en) * 1994-02-11 2006-01-24 Invitrogen Corporation Reagents for intracellular delivery of macromolecules
US5733762A (en) * 1994-04-28 1998-03-31 I.D.M. Immuno-Designed Molecules Complexes of nucleic acid and polymer, their process of preparation and their use for the transfection of cells
US5795587A (en) * 1995-01-23 1998-08-18 University Of Pittsburgh Stable lipid-comprising drug delivery complexes and methods for their production
US20030069173A1 (en) * 1998-03-16 2003-04-10 Life Technologies, Inc. Peptide-enhanced transfections
US5780448A (en) * 1995-11-07 1998-07-14 Ottawa Civic Hospital Loeb Research DNA-based vaccination of fish
EP0775751A3 (en) * 1995-11-22 1998-06-10 F. Hoffmann-La Roche Ag Cell transfection
US20010007771A1 (en) * 1996-05-29 2001-07-12 Sean M. Sullivan Cationic polymers and lipids for the delivery of nucleic acids
US5948681A (en) * 1996-08-14 1999-09-07 Children's Hospital Of Philadelphia Non-viral vehicles for use in gene transfer
ATE331030T1 (de) * 1996-11-04 2006-07-15 Ottawa Health Research Inst Verfahren zur expression von polypeptide in fishen durch wässerung in dns enthaltende lösung
FR2755976B1 (fr) * 1996-11-15 1999-01-15 Idm Immuno Designed Molecules Nouveaux complexes d'acides nucleiques et de polymere substitue par des residus entrainant la destabilisation des membranes cellulaires
US6919076B1 (en) 1998-01-20 2005-07-19 Pericor Science, Inc. Conjugates of agents and transglutaminase substrate linking molecules
US6958148B1 (en) 1998-01-20 2005-10-25 Pericor Science, Inc. Linkage of agents to body tissue using microparticles and transglutaminase
AU2868099A (en) 1998-02-13 1999-08-30 Selective Genetics, Inc. Concurrent flow mixing methods and apparatuses for the preparation of gene therapy vectors and compositions prepared thereby
EP1129064B1 (en) * 1998-11-12 2008-01-09 Invitrogen Corporation Transfection reagents
US6358697B2 (en) 1999-04-21 2002-03-19 Children's Hospital Medical Center Intracellular pharmaceutical targeting
FR2797402B1 (fr) 1999-07-15 2004-03-12 Biomerieux Stelhys Utilisation d'un polypeptide pour detecter, prevenir ou traiter un etat pathologique associe a une maladie degenerative, neurologique ou autoimmune
IL131074A0 (en) 1999-07-23 2001-03-19 Polygene Ltd A biodegradable polycation composition for delivery of an anionic macromolecule
US7713739B1 (en) * 2000-11-17 2010-05-11 Novartis Vaccines And Diagnostics, Inc. Microparticle-based transfection and activation of dendritic cells
WO2001036599A1 (en) * 1999-11-19 2001-05-25 Chiron Corporation Microparticle-based transfection and activation of dendritic cells
CA2363180A1 (en) 1999-12-28 2001-07-05 Transgene S.A. Use of lithium (li+) for the preparation of a composition for transfection of a polynucleotide into a cell and compositions useful in gene therapy
AU2001238485A1 (en) * 2000-02-18 2001-08-27 The Immune Response Corporation Methods and compositions for gene delivery
MXPA02008637A (es) 2000-03-02 2004-09-06 Ml Lab Plc Elemento de respuesta a factor de celulas t.
CA2421391A1 (en) * 2000-09-08 2002-03-14 Mekbib Astatke Compositions and methods for enhanced sensitivity and specificity of nucleic acid synthesis
CN1671400A (zh) * 2002-07-03 2005-09-21 派瑞克科学公司 透明质酸组合物以及使用方法
JP2006517790A (ja) * 2003-01-09 2006-08-03 インヴィトロジェン コーポレーション ポリペプチド−核酸複合体の細胞の送達および活性化
US20050031579A1 (en) * 2003-06-30 2005-02-10 Canji, Inc. Polymer encapsulation of adenoviruses
WO2008036929A2 (en) * 2006-09-21 2008-03-27 Alnylam Pharmaceuticals, Inc. Complex for transferring an anionic substance into a cell
JP5491396B2 (ja) * 2007-09-11 2014-05-14 モンドバイオテック ラボラトリーズ アクチエンゲゼルシャフト 治療剤としてのデフェンシンペプチドの使用
GB0724253D0 (en) * 2007-12-12 2008-01-30 Fermentas Uab Transfection reagent
FR2928373B1 (fr) * 2008-03-05 2010-12-31 Centre Nat Rech Scient Polymere derive de la polyethylenimine lineaire pour le transfert de gene.
US9856456B2 (en) 2009-10-12 2018-01-02 Thermo Fisher Scientific Baltics Uab Delivery agent
WO2011117352A1 (en) 2010-03-24 2011-09-29 Institut National De La Sante Et De La Recherche Medicale (Inserm) Primate model from the family cercopithecidae infected by a hbv strain of human genotype
US10195280B2 (en) 2014-07-15 2019-02-05 Life Technologies Corporation Compositions and methods for efficient delivery of molecules to cells
US11826423B2 (en) 2016-11-16 2023-11-28 Immunomic Therapeutics, Inc. Nucleic acids for treatment of allergies
WO2018195527A1 (en) 2017-04-22 2018-10-25 Immunomic Therapeutics, Inc. Improved lamp constructs
KR20200016224A (ko) 2017-05-02 2020-02-14 이뮤노믹 쎄라퓨틱스, 인크. 암 항원을 포함하는 lamp (리소솜 연관 막 단백질) 구축물
US12398195B2 (en) 2018-05-15 2025-08-26 Immunomic Therapeutics, Inc. Lamp constructs comprising allergens
TWI852977B (zh) 2019-01-10 2024-08-21 美商健生生物科技公司 前列腺新抗原及其用途
KR20220083773A (ko) 2019-10-18 2022-06-20 이뮤노믹 쎄라퓨틱스, 인크. 암 항원을 포함하는 개선된 lamp 구축물
EP4061405A1 (en) 2019-11-18 2022-09-28 Janssen Biotech, Inc. Vaccines based on mutant calr and jak2 and their uses
EP4175664A2 (en) 2020-07-06 2023-05-10 Janssen Biotech, Inc. Prostate neoantigens and their uses
US20230035403A1 (en) 2020-07-06 2023-02-02 Janssen Biotech, Inc. Method For Determining Responsiveness To Prostate Cancer Treatment
EP4175721A1 (en) 2020-07-06 2023-05-10 Janssen Biotech, Inc. Prostate neoantigens and their uses
EP4508071A1 (en) 2022-04-10 2025-02-19 Immunomic Therapeutics, Inc. Bicistronic lamp constructs comprising immune response enhancing genes and methods of use thereof

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DE4104186A1 (de) * 1991-02-12 1992-08-13 Genentech Inc Neue, ueber endozytose in hoehere eukaryotische zellen aufnehmbare, nukleinsaeure enthaltende komplexe

Also Published As

Publication number Publication date
CA2187629A1 (fr) 1995-11-09
EP0753070B1 (fr) 2002-09-25
US5595897A (en) 1997-01-21
CA2187629C (fr) 2004-09-21
DE69528363D1 (de) 2002-10-31
AU695056B2 (en) 1998-08-06
FR2719316A1 (fr) 1995-11-03
AU2412895A (en) 1995-11-29
ATE224957T1 (de) 2002-10-15
FR2719316B1 (fr) 1996-05-31
EP0753070A1 (fr) 1997-01-15
WO1995030020A1 (fr) 1995-11-09
DE69528363T2 (de) 2003-05-15

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