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ES2177632T3 - Purificacion de anticuerpos. - Google Patents

Purificacion de anticuerpos.

Info

Publication number
ES2177632T3
ES2177632T3 ES95910250T ES95910250T ES2177632T3 ES 2177632 T3 ES2177632 T3 ES 2177632T3 ES 95910250 T ES95910250 T ES 95910250T ES 95910250 T ES95910250 T ES 95910250T ES 2177632 T3 ES2177632 T3 ES 2177632T3
Authority
ES
Spain
Prior art keywords
purification
antibodies
chromatography
application
relates
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES95910250T
Other languages
English (en)
Inventor
Paula J Shadle
John C Erickson
Robert G Scott
Thomas M Smith
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SmithKline Beecham Corp
Original Assignee
SmithKline Beecham Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=22740445&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2177632(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by SmithKline Beecham Corp filed Critical SmithKline Beecham Corp
Application granted granted Critical
Publication of ES2177632T3 publication Critical patent/ES2177632T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B01D15/325—Reversed phase
    • B01D15/327—Reversed phase with hydrophobic interaction
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G or L chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/08—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses
    • C07K16/10—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses from RNA viruses
    • C07K16/1027—Paramyxoviridae, e.g. respiratory syncytial virus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C07K16/2812—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against CD4

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Pulmonology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Detergent Compositions (AREA)

Abstract

ESTA INVENCION SE REFIERE A LA APLICACION DE LA CROMATOGRAFIA COMBINADA DE LA CROMATOGRAFIA DE INTERACCION HIDROFOBICA A LA PURIFICACION DE PROTEINAS DE MOLECULAS DE ANTICUERPOS. EL PROCESO PUEDE INCLUIR LOS PASOS SUCESIVOS DE CROMATOGRAFIA DE AFINIDAD A PROTEINA A, CROMATOGRAFIA DE INTERCAMBIO DE IONES Y CROMATOGRAFIA DE INTERACCION HIDROFOBICA. POR ESTE PROCESO SE PUEDE RECUPERAR EL ANTICUERPO MONOMERICO PURIFICADO IGG, LIBRE DE AGREGADOS DE INMUNOGLOBULINA, ESPECIES MAL ENLAZADAS, PROTEINA DE CELULA ANFITRIONA Y PROTEINA A.
ES95910250T 1994-02-22 1995-02-21 Purificacion de anticuerpos. Expired - Lifetime ES2177632T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/200,126 US5429746A (en) 1994-02-22 1994-02-22 Antibody purification

Publications (1)

Publication Number Publication Date
ES2177632T3 true ES2177632T3 (es) 2002-12-16

Family

ID=22740445

Family Applications (1)

Application Number Title Priority Date Filing Date
ES95910250T Expired - Lifetime ES2177632T3 (es) 1994-02-22 1995-02-21 Purificacion de anticuerpos.

Country Status (21)

Country Link
US (4) US5429746A (es)
EP (1) EP0746398B1 (es)
JP (1) JP4198751B2 (es)
KR (1) KR100372209B1 (es)
CN (1) CN1146730A (es)
AT (1) ATE218387T1 (es)
AU (1) AU689552B2 (es)
BR (1) BR9507100A (es)
CA (1) CA2183888C (es)
CZ (1) CZ292001B6 (es)
DE (1) DE69526929T2 (es)
DK (1) DK0746398T3 (es)
ES (1) ES2177632T3 (es)
HU (1) HU217850B (es)
MX (1) MX9603637A (es)
NO (1) NO319182B1 (es)
NZ (1) NZ281480A (es)
PT (1) PT746398E (es)
SI (1) SI0746398T1 (es)
WO (1) WO1995022389A1 (es)
ZA (1) ZA951372B (es)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2531459A1 (es) * 2013-06-10 2015-03-16 Antonio VILA SANJURJO Método para la separación física de "traductomas" convolucionados

Families Citing this family (169)

* Cited by examiner, † Cited by third party
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