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ES2173812A1 - Dermis artificial y metodo de obtencion. - Google Patents

Dermis artificial y metodo de obtencion.

Info

Publication number
ES2173812A1
ES2173812A1 ES200100494A ES200100494A ES2173812A1 ES 2173812 A1 ES2173812 A1 ES 2173812A1 ES 200100494 A ES200100494 A ES 200100494A ES 200100494 A ES200100494 A ES 200100494A ES 2173812 A1 ES2173812 A1 ES 2173812A1
Authority
ES
Spain
Prior art keywords
artificial
artificial dermis
skin
plasma
dermis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES200100494A
Other languages
English (en)
Other versions
ES2173812B1 (es
Inventor
Noval Jose Luis Jorcado
Laguzzi Fernando Larcher
Infiesta Alvaro Meana
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
CENTYRO COMUNITARIO DE TRANSFU
MEDIOAMBIENTALES Y TECNOLOGICA
Centro de Investigaciones Energeticas Medioambientales y Tecnologicas CIEMAT
Original Assignee
CENTYRO COMUNITARIO DE TRANSFU
MEDIOAMBIENTALES Y TECNOLOGICA
Centro de Investigaciones Energeticas Medioambientales y Tecnologicas CIEMAT
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by CENTYRO COMUNITARIO DE TRANSFU, MEDIOAMBIENTALES Y TECNOLOGICA, Centro de Investigaciones Energeticas Medioambientales y Tecnologicas CIEMAT filed Critical CENTYRO COMUNITARIO DE TRANSFU
Priority to ES200100494A priority Critical patent/ES2173812B1/es
Priority to TW091103519A priority patent/TWI258506B/zh
Priority to EP09014041A priority patent/EP2165678A1/en
Priority to ARP020100722A priority patent/AR032916A1/es
Priority to CA2439387A priority patent/CA2439387C/en
Priority to JP2002571856A priority patent/JP4235452B2/ja
Priority to EP02702424A priority patent/EP1375647B1/en
Priority to DE60234323T priority patent/DE60234323D1/de
Priority to PT02702424T priority patent/PT1375647E/pt
Priority to AU2002235958A priority patent/AU2002235958B2/en
Priority to US10/469,554 priority patent/US7244552B2/en
Priority to PCT/ES2002/000087 priority patent/WO2002072800A1/es
Priority to ES02702424T priority patent/ES2336430T3/es
Priority to AT02702424T priority patent/ATE448295T1/de
Priority to DK02702424.9T priority patent/DK1375647T3/da
Priority to EG20020229A priority patent/EG23321A/xx
Publication of ES2173812A1 publication Critical patent/ES2173812A1/es
Application granted granted Critical
Publication of ES2173812B1 publication Critical patent/ES2173812B1/es
Priority to US11/809,665 priority patent/US20070275461A1/en
Priority to JP2008118300A priority patent/JP5047047B2/ja
Priority to US13/367,548 priority patent/US9168125B2/en
Priority to JP2012107526A priority patent/JP5529918B2/ja
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
    • C12N5/0698—Skin equivalents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
    • A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
    • A61F2/02—Prostheses implantable into the body
    • A61F2/10—Hair or skin implants
    • A61F2/105—Skin implants, e.g. artificial skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/33—Fibroblasts
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/36—Skin; Hair; Nails; Sebaceous glands; Cerumen; Epidermis; Epithelial cells; Keratinocytes; Langerhans cells; Ectodermal cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/60—Materials for use in artificial skin

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Epidemiology (AREA)
  • Transplantation (AREA)
  • Medicinal Chemistry (AREA)
  • Dermatology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Cardiology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Vascular Medicine (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Materials For Medical Uses (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

Dermis artificial y método de obtención. Dermis artificial (1) obtenida a partir de plasma con plaquetas (2) y fibroblastos (3) humanos. El plasma con plaquetas (2) se obtiene por fraccionamiento de la sangre total (4) del paciente (8) mediante centrifugación ligera, y los fibroblastos (3) humanos a partir de una biopsia de piel (5). La gelificación se consigue mediante la adición de calcio. Esta dermis artificial (1) permite un crecimiento rápido de los queratinocitos (6) sembrados en su superficie para constituir una piel artificial (7) que es fácilmente trasplantable. Se consiguen grandes superficies de dermis artificial (1) a partir de una pequeña biopsia de piel (5) y de cantidades mínimas de plasma con plaquetas (2), que al estar enriquecido en citoquinas y factores de crecimiento plaquetario potencia la proliferación de las células sembradas, tanto en su interior como en su superficie. La piel artificial (7) así obtenida puede servir para el tratamiento de grandes quemados, úlceras cutáneas crónicas... o utilizarse, mediante el empleo de células genéticamente modificadas, como base para terapia génica.
ES200100494A 2001-03-01 2001-03-01 Dermis artificial y metodo de obtencion. Expired - Fee Related ES2173812B1 (es)

Priority Applications (20)

Application Number Priority Date Filing Date Title
ES200100494A ES2173812B1 (es) 2001-03-01 2001-03-01 Dermis artificial y metodo de obtencion.
TW091103519A TWI258506B (en) 2001-03-01 2002-02-27 Artificial dermis and method of preparation
AT02702424T ATE448295T1 (de) 2001-03-01 2002-02-28 Künstliche dermis und herstellungsverfahren dafür
CA2439387A CA2439387C (en) 2001-03-01 2002-02-28 Artificial dermis and production method therefor
JP2002571856A JP4235452B2 (ja) 2001-03-01 2002-02-28 人工真皮及びその製造方法
EP02702424A EP1375647B1 (en) 2001-03-01 2002-02-28 Artificial dermis and production method therefor
DE60234323T DE60234323D1 (de) 2001-03-01 2002-02-28 Künstliche dermis und herstellungsverfahren dafür
PT02702424T PT1375647E (pt) 2001-03-01 2002-02-28 Derme artificial e respectivo método de produção
AU2002235958A AU2002235958B2 (en) 2001-03-01 2002-02-28 Artificial dermis and production method therefor
US10/469,554 US7244552B2 (en) 2001-03-01 2002-02-28 Artificial dermis and production method therefor
EP09014041A EP2165678A1 (en) 2001-03-01 2002-02-28 Artificial dermis and production method therefor
ES02702424T ES2336430T3 (es) 2001-03-01 2002-02-28 Dermis artificial y metodo de obtecion.
ARP020100722A AR032916A1 (es) 2001-03-01 2002-02-28 Dermis artificial y metodo de obtencion.
DK02702424.9T DK1375647T3 (da) 2001-03-01 2002-02-28 Kunstig dermis og fremstillingsfremgangsmåde dertil
PCT/ES2002/000087 WO2002072800A1 (es) 2001-03-01 2002-02-28 Dermis artificial y metodo de obtencion
EG20020229A EG23321A (en) 2001-03-01 2002-03-02 Artificial dermis and method of preparation.
US11/809,665 US20070275461A1 (en) 2001-03-01 2007-05-31 Artificial dermis and method of preparation
JP2008118300A JP5047047B2 (ja) 2001-03-01 2008-04-30 人工真皮
US13/367,548 US9168125B2 (en) 2001-03-01 2012-02-07 Artificial dermis and method of preparation
JP2012107526A JP5529918B2 (ja) 2001-03-01 2012-05-09 人工真皮

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES200100494A ES2173812B1 (es) 2001-03-01 2001-03-01 Dermis artificial y metodo de obtencion.

Publications (2)

Publication Number Publication Date
ES2173812A1 true ES2173812A1 (es) 2002-10-16
ES2173812B1 ES2173812B1 (es) 2003-12-16

Family

ID=8496947

Family Applications (2)

Application Number Title Priority Date Filing Date
ES200100494A Expired - Fee Related ES2173812B1 (es) 2001-03-01 2001-03-01 Dermis artificial y metodo de obtencion.
ES02702424T Expired - Lifetime ES2336430T3 (es) 2001-03-01 2002-02-28 Dermis artificial y metodo de obtecion.

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES02702424T Expired - Lifetime ES2336430T3 (es) 2001-03-01 2002-02-28 Dermis artificial y metodo de obtecion.

Country Status (14)

Country Link
US (3) US7244552B2 (es)
EP (2) EP2165678A1 (es)
JP (3) JP4235452B2 (es)
AR (1) AR032916A1 (es)
AT (1) ATE448295T1 (es)
AU (1) AU2002235958B2 (es)
CA (1) CA2439387C (es)
DE (1) DE60234323D1 (es)
DK (1) DK1375647T3 (es)
EG (1) EG23321A (es)
ES (2) ES2173812B1 (es)
PT (1) PT1375647E (es)
TW (1) TWI258506B (es)
WO (1) WO2002072800A1 (es)

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* Cited by examiner, † Cited by third party
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US7144729B2 (en) 2000-09-01 2006-12-05 Dfb Pharmaceuticals, Inc. Methods and compositions for tissue regeneration
ES2173812B1 (es) * 2001-03-01 2003-12-16 Ct Investig Energeticas Ciemat Dermis artificial y metodo de obtencion.
JP2004075661A (ja) * 2002-06-18 2004-03-11 Shiseido Co Ltd 表皮基底膜ケアを特徴とする皮膚外用剤、皮膚基底膜構造形成促進剤および人工皮膚の製造方法
GB0224986D0 (en) 2002-10-28 2002-12-04 Smith & Nephew Apparatus
US11298453B2 (en) 2003-10-28 2022-04-12 Smith & Nephew Plc Apparatus and method for wound cleansing with actives
AU2005215213C1 (en) * 2004-02-13 2011-04-28 Smith & Nephew Orthopaedics Ag Pharmaceutical composition comprising a fibrin matrix and dermal fibroblasts for the treatment of wounds
WO2005084223A2 (en) * 2004-02-27 2005-09-15 The General Hospital Corporation Methods and compositions for hair growth
US7753894B2 (en) 2004-04-27 2010-07-13 Smith & Nephew Plc Wound cleansing apparatus with stress
GB0505202D0 (en) 2005-03-14 2005-04-20 Intercytex Ltd Skin equivalent culture
ES2263382B1 (es) * 2005-05-16 2007-11-16 Fundacion Para La Investigacion Biomedica Del Hospital Gregorio Marañon Matriz artificial de gel de fibrina endotelizada superproductora de factores proangiogenicos.
CA2609507C (en) 2005-05-26 2014-10-21 Intercytex Limited Tissue repair using allogenic dermal fibroblasts
WO2007098954A1 (en) * 2006-03-03 2007-09-07 Ciemat A mouse model comprising an engrafted human skin having hypersensitivity to uv-light
US8709081B2 (en) 2006-10-23 2014-04-29 Stemedica Cell Technologies, Inc. Cellular scaffold
US8105380B2 (en) 2006-10-23 2012-01-31 Stemedica Cell Technologies, Inc. Cellular scaffold
US20100303770A1 (en) * 2006-12-28 2010-12-02 John Maslowski Methods for culturing dermal cells for treatment of skin injuries such as burns
JP5253749B2 (ja) * 2007-03-19 2013-07-31 肇 井上 表皮シートの作製方法および初代培養時における培養容器への上皮幹細胞の接着性付与方法
CA2701884A1 (en) * 2007-10-15 2009-04-23 Wake Forest University Health Sciences Methods and compositions for printing biologically compatible nanotube composites of autologous tissue
WO2009107266A1 (ja) * 2008-02-29 2009-09-03 学校法人昭和大学 人工皮膚の製造方法
US20110318404A1 (en) 2009-01-27 2011-12-29 Alon Kushnir Wound Dressing, Methods and Apparatus For Making Same and Storage and Use Thereof
WO2011023843A2 (es) * 2009-08-25 2011-03-03 Servicio Andaluz De Salud Elaboración de tejidos artificiales mediante ingeniería tisular utilizando biomateriales de fibrina y agarosa
JP5737860B2 (ja) * 2010-04-30 2015-06-17 テルモ株式会社 ゲル状細胞組成物およびその製造方法
CL2011001870A1 (es) * 2011-08-03 2012-01-27 U Tecnica Federico Santa Maria 28 71% Proceso de preparacion de un gel de fibrina autologo o desde sangre compatible para proliferacion y vehiculizacion celular; gel de fibrina o de sangre compatible; uso del gel de fibrina o de sangre compatible para uso quirurgico.
TWI448310B (zh) * 2012-05-23 2014-08-11 Medical dressings and their coating methods for frozen freeze - dried platelets
TWI448312B (zh) * 2012-05-23 2014-08-11 An implant of freeze - dried platelets and its coating method
GB201303485D0 (en) 2013-02-27 2013-04-10 Alcyomics Ltd Skin model
MX342233B (es) * 2014-02-04 2016-08-24 Alvaro Galue Eduardo Proceso de obtencion de un compuesto de aspersion celular de fibroblastos y queratinocitos humanos en solucion y su uso como agente regenerativo de lesiones cutáneas.
ES2551143B1 (es) * 2014-05-13 2016-07-01 Fundación Tekniker Dermis artificial, piel artificial, métodos para su preparación y sus usos
CN105505855A (zh) * 2016-02-04 2016-04-20 关志广 一种具备增殖能力的自体表皮细胞apg凝胶的制作方法
MX2018014889A (es) * 2016-05-31 2019-04-08 Octapharma Ag Peliculas a base de plasma y metodos para elaborar y usar las mismas.

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0373044A1 (fr) * 1988-12-06 1990-06-13 Centre Regional De Transfusion Sanguine De Lille Support biologique pour cultures cellulaires constitué de protéines plasmatiques coagulées par la thrombine, son utilisation pour la culture des kératinocytes, leur récupération et leur transport à des fins d'utilisation thérapeutique
ES2117573A1 (es) * 1996-07-19 1998-08-01 Comunitario De Transfusion Del Base dermica especialmente diseñada para el cultivo de queratinocitos.
JPH10277143A (ja) * 1997-04-08 1998-10-20 Tokai Univ 移植片およびその製造方法
ES2132027A1 (es) * 1997-07-04 1999-08-01 Comunitario De Transfusion Del Desarrollo de una piel artificial mediante cultivo de queratinocitos sobre una base de fibrina y fibroblastos humanos y metodo de preparacion de esta piel para trasplante.

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JPS6379588A (ja) * 1986-09-25 1988-04-09 Yasuo Moriya 細胞培養用基材およびその製造方法
DK475386D0 (da) * 1986-10-03 1986-10-03 Weis Fogh Ulla Sivertsen Fremgangsmaade og apparat til fremstilling af biologiske stoffer
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JP3091113B2 (ja) * 1994-11-22 2000-09-25 科学技術振興事業団 生理活性物質分泌性のハイブリッド型ゲル
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WO1997001533A1 (en) 1995-06-27 1997-01-16 The Green Cross Corporation Thiocarbamic acid derivatives
WO2000062828A1 (en) * 1996-04-30 2000-10-26 Medtronic, Inc. Autologous fibrin sealant and method for making the same
JPH11239609A (ja) * 1998-02-26 1999-09-07 Sekisui Chem Co Ltd 血小板含有生体組織用接着剤
JPH11246420A (ja) * 1998-02-26 1999-09-14 Sekisui Chem Co Ltd 創傷治癒促進剤
ES2173812B1 (es) * 2001-03-01 2003-12-16 Ct Investig Energeticas Ciemat Dermis artificial y metodo de obtencion.
ES2184623B1 (es) * 2001-06-29 2004-09-16 Centro De Investigaciones Energeticas, Medioambientales Y Tecnologicas (C.I.E.M.A.T.) Piel artificial autologa secretora de leptina y metodo de obtencion.
US20030166274A1 (en) * 2001-11-15 2003-09-04 Hewitt Charles W. Three-dimensional matrix for producing living tissue equivalents
US20040126881A1 (en) * 2002-09-06 2004-07-01 Vincent Ronfard Fibrin cell supports and methods of use thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0373044A1 (fr) * 1988-12-06 1990-06-13 Centre Regional De Transfusion Sanguine De Lille Support biologique pour cultures cellulaires constitué de protéines plasmatiques coagulées par la thrombine, son utilisation pour la culture des kératinocytes, leur récupération et leur transport à des fins d'utilisation thérapeutique
ES2117573A1 (es) * 1996-07-19 1998-08-01 Comunitario De Transfusion Del Base dermica especialmente diseñada para el cultivo de queratinocitos.
JPH10277143A (ja) * 1997-04-08 1998-10-20 Tokai Univ 移植片およびその製造方法
ES2132027A1 (es) * 1997-07-04 1999-08-01 Comunitario De Transfusion Del Desarrollo de una piel artificial mediante cultivo de queratinocitos sobre una base de fibrina y fibroblastos humanos y metodo de preparacion de esta piel para trasplante.

Also Published As

Publication number Publication date
EP2165678A1 (en) 2010-03-24
DK1375647T3 (da) 2010-03-15
EP1375647A1 (en) 2004-01-02
JP2008253788A (ja) 2008-10-23
TWI258506B (en) 2006-07-21
US9168125B2 (en) 2015-10-27
DE60234323D1 (de) 2009-12-24
JP4235452B2 (ja) 2009-03-11
CA2439387A1 (en) 2002-09-19
JP5529918B2 (ja) 2014-06-25
US7244552B2 (en) 2007-07-17
ES2336430T3 (es) 2010-04-13
US20120183505A1 (en) 2012-07-19
ES2173812B1 (es) 2003-12-16
EP1375647B1 (en) 2009-11-11
JP2012187412A (ja) 2012-10-04
US20040171145A1 (en) 2004-09-02
WO2002072800A1 (es) 2002-09-19
JP2004522545A (ja) 2004-07-29
AU2002235958B2 (en) 2007-05-10
ATE448295T1 (de) 2009-11-15
US20070275461A1 (en) 2007-11-29
JP5047047B2 (ja) 2012-10-10
AR032916A1 (es) 2003-12-03
EG23321A (en) 2004-12-29
PT1375647E (pt) 2010-02-18
CA2439387C (en) 2012-04-24

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