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ES2082787T3 - Deacetoxicefalosporina c sintasa purificada. - Google Patents

Deacetoxicefalosporina c sintasa purificada.

Info

Publication number
ES2082787T3
ES2082787T3 ES89310744T ES89310744T ES2082787T3 ES 2082787 T3 ES2082787 T3 ES 2082787T3 ES 89310744 T ES89310744 T ES 89310744T ES 89310744 T ES89310744 T ES 89310744T ES 2082787 T3 ES2082787 T3 ES 2082787T3
Authority
ES
Spain
Prior art keywords
deacetoxicefalosporina
purificada
sintasa
recombinantly
sintae
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES89310744T
Other languages
English (en)
Inventor
Joe Edward Dotzlaf
Wu-Kuang Yeh
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eli Lilly and Co
Original Assignee
Eli Lilly and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eli Lilly and Co filed Critical Eli Lilly and Co
Application granted granted Critical
Publication of ES2082787T3 publication Critical patent/ES2082787T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/886—Streptomyces

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

EL SINTASE-C DE ACETOXICEFALOSPORIN OBTENIDO DE CELULAS LIBRES EXTRAIDAS DE STREPTOMYCES CLAVULIGERUS ES PURIFICADO POR HOMEGENIDAD DIRECTA Y CARACTERIZADO EN FORMA INACTIVA. LA SECUENCIA DE ACIDO AMINO ES DETERMINADA Y LA SINTASE RECOMBINANTEMENTE REPRODUCIDA EN FORMA ACTIVA EN E. COLI. LA SINTASE PRODUCIDA RECOMBINANTEMENTE ES PROVISTA EN APROXIMADAMENTE EL 97 % DE FORMA PURA POR UN PROCESO CROMATOGRAFICO. LA FUNCION HIDROXILASE DAOC CARECE DE SINTAE; SIN EMBARGO, POSEE LA CAPACIDAD PARA TRANSFORMAR EL C EXOMETILENECEFALOSPORIN-3 A C DEACETILCEFALOSPORIN.
ES89310744T 1988-10-24 1989-10-19 Deacetoxicefalosporina c sintasa purificada. Expired - Lifetime ES2082787T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/261,437 US5082772A (en) 1988-10-24 1988-10-24 Process for preparing deacetylcephalosporin c

Publications (1)

Publication Number Publication Date
ES2082787T3 true ES2082787T3 (es) 1996-04-01

Family

ID=22993294

Family Applications (1)

Application Number Title Priority Date Filing Date
ES89310744T Expired - Lifetime ES2082787T3 (es) 1988-10-24 1989-10-19 Deacetoxicefalosporina c sintasa purificada.

Country Status (8)

Country Link
US (1) US5082772A (es)
EP (1) EP0366354B1 (es)
JP (1) JPH02242675A (es)
DE (1) DE68925338T2 (es)
DK (1) DK521489A (es)
ES (1) ES2082787T3 (es)
GR (1) GR3018596T3 (es)
IL (1) IL92079A (es)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0233715B1 (en) * 1986-01-31 1994-05-25 BEECHAM GROUP plc Isolation and expression of genes involved in the biosynthesis of beta-lactams
US6709859B1 (en) 1986-01-31 2004-03-23 Beecham Group P.L.C. Chromosomal DNA Fragments Encoding Enzymes for Encoding B-Lactam Biosynthesis Enzymes, and Vector and Transformants for Their Expression
UA47385C2 (uk) * 1991-10-15 2002-07-15 Гіст-Брокейдс Б.В. Спосіб одержання 7-амінодеацетилцефалоспоранової кислоти, спосіб одержання 7-аміноцефалоспоранової кислоти, вектор експресії рекомбінантної днк (варіанти), клітина-хазяїн penicillium chrysogenum (варіанти), спосіб культивування рекомбінантної клітини-хазяїна penicillium chrysogenum (варіанти)
HU219259B (en) * 1993-07-30 2001-03-28 Gist Brocades Bv Process for the efficient production of 7-adca via 2-(carboxyethylthio)acetyl-7-adca and 3-(carboxymethylthio)propionyl-7-adca, recombinant dna vectrors and transformed host cells
BR9407104A (pt) * 1993-07-30 1996-08-27 Gist Brocades Nv Processo para a preparação e recuperação de ácido 7-amino desacetoxi cefalosporânico (7-adca) vetor de ADN recombinante e célula hospedeira transformada com um vetor definido
RU2139349C1 (ru) * 1993-07-30 1999-10-10 Гист-Брокейдс Б.В. Способ эффективного производства 7-адцк через 2-(карбоксиэтилтио) ацетил-7-адцк и 3-(карбоксиметилтио)пропионил-7-адцк
US5571910A (en) * 1994-12-09 1996-11-05 Schering Corporation Process for the preparation of intermediates useful in the synthesis of cephalosporins
US5731165A (en) * 1995-06-02 1998-03-24 Gist-Brocades, B.V. Process for the production of 7-ADCA via expandase activity on penicillin G
US5919680A (en) * 1995-11-27 1999-07-06 Isis Innovation Limited Process for the production of SSC's via expandase activity on penicillin G
WO1998002551A2 (en) * 1996-07-16 1998-01-22 Gist-Brocades B.V. Process for the production of adipoyl cephalosporins
AU1772099A (en) * 1997-12-24 1999-07-19 Isis Innovation Limited Modified deacetoxycephalosporin c synthase (daocs) and x-ray structure
US6383773B2 (en) * 1998-04-23 2002-05-07 Massachusetts Institute Of Technology Penicillin conversion
KR100648480B1 (ko) 1998-05-19 2006-11-24 디에스엠 아이피 어셋츠 비.브이. 세팔로스포린의 개선된 생체내 생산
DE10051628B4 (de) * 2000-10-18 2007-06-06 Fresenius Hemocare Beteiligungs Gmbh Einzelsträngiges Oligonukleotid und dessen Verwendung
US6699699B2 (en) 2002-03-26 2004-03-02 Synmax Biochemical Co., Ltd. Mutated penicillin expandases
CN113234093A (zh) * 2021-04-13 2021-08-10 伊犁川宁生物技术股份有限公司 一种3-去乙酰氧基头孢菌素c钠盐及其制备方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3847742A (en) * 1972-04-26 1974-11-12 C Higgens Process for deacetoxycephalosporin c
JPS5539735A (en) * 1978-09-11 1980-03-19 Takeda Chem Ind Ltd Preparation of deacetyl cephalosporin c
US4414328A (en) * 1980-07-21 1983-11-08 Fujisawa Pharmaceutical Co., Ltd. Process for the preparation of deacetylcephalosporin C
US4510246A (en) * 1982-08-23 1985-04-09 Queen's University At Kingston Isolation of cyclase, epimerase and a ring expansion enzyme for producing unnatural cephalosporins
US4536476A (en) * 1982-08-23 1985-08-20 Queen's University At Kingston Stable epimerase reagent, cyclase reagent and ring expansion reagent for cell-free production of cephalosporins
US4693977A (en) * 1982-08-23 1987-09-15 Queen's University At Kingston Enzyme immobilization for producing cephalosporin antibiotics
EP0233715B1 (en) * 1986-01-31 1994-05-25 BEECHAM GROUP plc Isolation and expression of genes involved in the biosynthesis of beta-lactams

Also Published As

Publication number Publication date
US5082772A (en) 1992-01-21
IL92079A (en) 1994-08-26
EP0366354A3 (en) 1991-03-20
GR3018596T3 (en) 1996-04-30
EP0366354B1 (en) 1996-01-03
DK521489A (da) 1990-04-25
DK521489D0 (da) 1989-10-20
JPH02242675A (ja) 1990-09-27
EP0366354A2 (en) 1990-05-02
DE68925338D1 (de) 1996-02-15
DE68925338T2 (de) 1996-06-05

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