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ES2041264T3 - Electrodos de enzima inmovilizada. - Google Patents

Electrodos de enzima inmovilizada.

Info

Publication number
ES2041264T3
ES2041264T3 ES198787304688T ES87304688T ES2041264T3 ES 2041264 T3 ES2041264 T3 ES 2041264T3 ES 198787304688 T ES198787304688 T ES 198787304688T ES 87304688 T ES87304688 T ES 87304688T ES 2041264 T3 ES2041264 T3 ES 2041264T3
Authority
ES
Spain
Prior art keywords
enzyme
adsorbed
carbon
immobilized
layer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES198787304688T
Other languages
English (en)
Inventor
Hugh Peter Bennetto
Gerard Michael Delaney
Jeremy Richard Mason
Christopher Frank Thurston
John Laing Stirling
David Robert De Keyzer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cambridge Life Sciences PLC
Original Assignee
Cambridge Life Sciences PLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cambridge Life Sciences PLC filed Critical Cambridge Life Sciences PLC
Application granted granted Critical
Publication of ES2041264T3 publication Critical patent/ES2041264T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001Enzyme electrodes
    • C12Q1/002Electrode membranes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001Enzyme electrodes
    • C12Q1/005Enzyme electrodes involving specific analytes or enzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001Enzyme electrodes
    • C12Q1/005Enzyme electrodes involving specific analytes or enzymes
    • C12Q1/006Enzyme electrodes involving specific analytes or enzymes for glucose
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00Chemistry: molecular biology and microbiology
    • Y10S435/817Enzyme or microbe electrode

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Emergency Medicine (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Inert Electrodes (AREA)
  • Catalysts (AREA)

Abstract

SE PRESENTAN ELECTRODOS DE ENZIMAS QUE SON CAPACES DE RESPONDER AMPERIMETRICAMENTE A LA ACTIVIDAD CATALITICA DE LA ENZIMA EN PRESENCIA DE SU RESPECTIVO SUSTRATO Y QUE COMPRENDEN LA ENZIMA INMOVILIZADA O ADSORBIDA EN LA SUPERFICIE DE UN ELEMENTO DE SOPORTE CONDUCTOR ELECTRICO QUE CONSTA DE, O COMPRENDE, UNA CAPA POROSA DE PARTICULAS DE CARBONO O DE GRAFITO UNIDAS A UNA RESINA, HABIENDO MEZCLADO INTIMAMENTE DICHAS PARTICULAS, O DEPOSITADO O ADSORBIDO EN LA SUPERFICIE DE LAS PARTICULAS INDIVIDUALES, ANTES DE UNIRLAS PARA FORMAR LA MENCIONADA CAPA, UN METAL DEL GRUPO DEL PLATINO, FINAMENTE DIVIDIDO, PARA FORMAR ASI UNA CAPA-SUSTRATO POROSA SOBRE LA CUAL SE ADSORBE O SE INMOVILIZA LA ENZIMA Y QUE COMPRENDE UNA CAPA SUSTANCIALMENTE HETEROGENEA DE PARTICULAS DE CARBONO O DE GRAFITO UNIDA A UNA RESINA, CON EL MENCIONADO METAL DEL GRUPO DEL PLATINO DISPERSADO DE MODO SUSTANCIALMENTE UNIFORME POR LA MENCIONADA CAPA. LOS MATERIALES DE SUSTRATO RECOMENDADOS SON ELECTRODOS DE PAPEL DE CARBONO PLATINADOUNIDO A UNA RESINA QUE COMPRENDE PARTICULAS DE POLVO DE CARBONO PLATINADO QUE TIENEN PLATINO COLOIDAL ADSORBIDO EN SU SUPERFICIE UNIDAS A UN SUSTRATO DE PAPEL DE CARBONO USANDO COMO LIGANTE UNA RESINA SINTETICA, PREFERIBLEMENTE POLITETRAFLUORETILENO. LOS ELECTRODOS DE ENZIMA RECOMENDADOS SON LOS DE GLUCOSAOXIDASA QUE COMPRENDEN GLUCOSAOXIDASA ADSORBIDA O INMOVILIZADA SOBRE LA SUPERFICIE DEL SUSTRATO.
ES198787304688T 1986-05-27 1987-05-27 Electrodos de enzima inmovilizada. Expired - Lifetime ES2041264T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB868612861A GB8612861D0 (en) 1986-05-27 1986-05-27 Immobilised enzyme biosensors

Publications (1)

Publication Number Publication Date
ES2041264T3 true ES2041264T3 (es) 1993-11-16

Family

ID=10598501

Family Applications (1)

Application Number Title Priority Date Filing Date
ES198787304688T Expired - Lifetime ES2041264T3 (es) 1986-05-27 1987-05-27 Electrodos de enzima inmovilizada.

Country Status (17)

Country Link
US (1) US4970145A (es)
EP (1) EP0247850B1 (es)
KR (1) KR950004906B1 (es)
AU (1) AU591565B2 (es)
CA (1) CA1303132C (es)
DE (1) DE3785485T2 (es)
DK (1) DK173722B1 (es)
ES (1) ES2041264T3 (es)
FI (1) FI88515C (es)
GB (2) GB8612861D0 (es)
HU (1) HU202577B (es)
IE (1) IE60371B1 (es)
IL (1) IL82601A (es)
MX (1) MX171340B (es)
NO (1) NO176920C (es)
RU (1) RU1801119C (es)
WO (1) WO1987007295A1 (es)

Families Citing this family (241)

* Cited by examiner, † Cited by third party
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FI88515C (fi) 1993-05-25
DK36188A (da) 1988-01-26
IL82601A (en) 1990-11-05
IE871360L (en) 1987-11-27
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MX171340B (es) 1993-10-20
IE60371B1 (en) 1994-07-13
AU591565B2 (en) 1989-12-07
EP0247850B1 (en) 1993-04-21
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DK36188D0 (da) 1988-01-26
DE3785485D1 (de) 1993-05-27
AU7436987A (en) 1987-12-22
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KR880701290A (ko) 1988-07-26
FI880300A7 (fi) 1988-01-22
US4970145A (en) 1990-11-13
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GB8612861D0 (en) 1986-07-02
FI880300A0 (fi) 1988-01-22
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WO1987007295A1 (en) 1987-12-03
RU1801119C (ru) 1993-03-07
HUT46056A (en) 1988-09-28
EP0247850A1 (en) 1987-12-02
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NO176920C (no) 1995-06-21
KR950004906B1 (ko) 1995-05-15
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CA1303132C (en) 1992-06-09
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IL82601A0 (en) 1987-11-30
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