EP4202058A4 - Élément de construction de banque compatible avec les plates-formes de double séquençage, kit et procédé de construction de banque - Google Patents
Élément de construction de banque compatible avec les plates-formes de double séquençage, kit et procédé de construction de banque Download PDFInfo
- Publication number
- EP4202058A4 EP4202058A4 EP21951111.0A EP21951111A EP4202058A4 EP 4202058 A4 EP4202058 A4 EP 4202058A4 EP 21951111 A EP21951111 A EP 21951111A EP 4202058 A4 EP4202058 A4 EP 4202058A4
- Authority
- EP
- European Patent Office
- Prior art keywords
- library construction
- kit
- method compatible
- construction method
- dual sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000010276 construction Methods 0.000 title 2
- 230000009977 dual effect Effects 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
- C12Q1/6855—Ligating adaptors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
- C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111322946.6A CN113999893B (zh) | 2021-11-09 | 2021-11-09 | 兼容双测序平台的建库元件、试剂盒及建库方法 |
| PCT/CN2021/131508 WO2023082305A1 (fr) | 2021-11-09 | 2021-11-18 | Élément de construction de banque compatible avec les plates-formes de double séquençage, kit et procédé de construction de banque |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| EP4202058A1 EP4202058A1 (fr) | 2023-06-28 |
| EP4202058A4 true EP4202058A4 (fr) | 2024-05-01 |
Family
ID=79928434
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP21951111.0A Pending EP4202058A4 (fr) | 2021-11-09 | 2021-11-18 | Élément de construction de banque compatible avec les plates-formes de double séquençage, kit et procédé de construction de banque |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US20240279647A1 (fr) |
| EP (1) | EP4202058A4 (fr) |
| CN (1) | CN113999893B (fr) |
| WO (1) | WO2023082305A1 (fr) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116004763B (zh) * | 2022-07-19 | 2024-02-09 | 纳昂达(南京)生物科技有限公司 | 一种组合型接头的选择验证和质控方法 |
| CN117551645A (zh) * | 2023-10-12 | 2024-02-13 | 江苏先声医学诊断有限公司 | 一种将Illumina文库快速转化为MGI测序平台文库的试剂及方法 |
Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018053070A1 (fr) * | 2016-09-14 | 2018-03-22 | Intellia Therapeutics, Inc. | Méthodes améliorées d'analyse d'adn édité |
| EP3382034A1 (fr) * | 2017-03-31 | 2018-10-03 | Rheinische Friedrich-Wilhelms-Universität Bonn | Analyse de l'expression génique par génération d'une librairie d'adnc circulaire simple |
| CN110114472A (zh) * | 2016-12-21 | 2019-08-09 | 深圳华大智造科技有限公司 | 将线性测序文库转换为环状测序文库的方法 |
| CN110511978A (zh) * | 2019-09-09 | 2019-11-29 | 北京优迅医学检验实验室有限公司 | Ffpe样本dna文库及其构建方法 |
| WO2020036991A1 (fr) * | 2018-08-15 | 2020-02-20 | Illumina, Inc. | Compositions et procédés pour améliorer l'enrichissement de bibliothèques |
| CN111118001A (zh) * | 2019-12-31 | 2020-05-08 | 苏州贝康医疗器械有限公司 | 一种多测序平台通用接头、适用于多测序平台的文库构建方法及试剂盒 |
| CN111748551A (zh) * | 2019-03-27 | 2020-10-09 | 纳昂达(南京)生物科技有限公司 | 封闭序列、捕获试剂盒、文库杂交捕获方法及建库方法 |
| WO2021016395A1 (fr) * | 2019-07-22 | 2021-01-28 | Igenomx International Genomics Corporation | Procédés et compositions pour une préparation d'échantillon à haut débit au moyen d'une double indexation double unique |
| CN112941147A (zh) * | 2021-03-02 | 2021-06-11 | 深圳市睿法生物科技有限公司 | 一种高保真靶标基因建库方法及其试剂盒 |
| CN113005121A (zh) * | 2021-04-25 | 2021-06-22 | 纳昂达(南京)生物科技有限公司 | 接头元件、试剂盒及其相关应用 |
| WO2021207267A1 (fr) * | 2020-04-07 | 2021-10-14 | Personal Genome Diagnostics Inc. | Codes à barres flottants |
| WO2021232023A2 (fr) * | 2020-05-15 | 2021-11-18 | Swift Biosciences, Inc. | Procédés de pcr couplée à une ligature |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070031865A1 (en) * | 2005-07-07 | 2007-02-08 | David Willoughby | Novel Process for Construction of a DNA Library |
| WO2018077847A1 (fr) * | 2016-10-31 | 2018-05-03 | F. Hoffmann-La Roche Ag | Construction de bibliothèque circulaire à code-barres pour l'identification de produits chimériques |
| CN109706219A (zh) * | 2018-12-20 | 2019-05-03 | 臻和（北京）科技有限公司 | 构建测序文库的方法、试剂盒、上机方法及测序数据的拆分方法 |
| CN112626189A (zh) * | 2020-04-24 | 2021-04-09 | 北京吉因加医学检验实验室有限公司 | 基因测序仪的短接头、双index接头引物和双index建库体系 |
| CN111910258B (zh) * | 2020-08-19 | 2021-06-15 | 纳昂达(南京)生物科技有限公司 | 双端文库标签组合物及其在mgi测序平台中的应用 |
-
2021
- 2021-11-09 CN CN202111322946.6A patent/CN113999893B/zh active Active
- 2021-11-18 US US18/016,857 patent/US20240279647A1/en active Pending
- 2021-11-18 EP EP21951111.0A patent/EP4202058A4/fr active Pending
- 2021-11-18 WO PCT/CN2021/131508 patent/WO2023082305A1/fr not_active Ceased
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018053070A1 (fr) * | 2016-09-14 | 2018-03-22 | Intellia Therapeutics, Inc. | Méthodes améliorées d'analyse d'adn édité |
| CN110114472A (zh) * | 2016-12-21 | 2019-08-09 | 深圳华大智造科技有限公司 | 将线性测序文库转换为环状测序文库的方法 |
| EP3382034A1 (fr) * | 2017-03-31 | 2018-10-03 | Rheinische Friedrich-Wilhelms-Universität Bonn | Analyse de l'expression génique par génération d'une librairie d'adnc circulaire simple |
| WO2020036991A1 (fr) * | 2018-08-15 | 2020-02-20 | Illumina, Inc. | Compositions et procédés pour améliorer l'enrichissement de bibliothèques |
| CN111748551A (zh) * | 2019-03-27 | 2020-10-09 | 纳昂达(南京)生物科技有限公司 | 封闭序列、捕获试剂盒、文库杂交捕获方法及建库方法 |
| WO2021016395A1 (fr) * | 2019-07-22 | 2021-01-28 | Igenomx International Genomics Corporation | Procédés et compositions pour une préparation d'échantillon à haut débit au moyen d'une double indexation double unique |
| CN110511978A (zh) * | 2019-09-09 | 2019-11-29 | 北京优迅医学检验实验室有限公司 | Ffpe样本dna文库及其构建方法 |
| CN111118001A (zh) * | 2019-12-31 | 2020-05-08 | 苏州贝康医疗器械有限公司 | 一种多测序平台通用接头、适用于多测序平台的文库构建方法及试剂盒 |
| WO2021207267A1 (fr) * | 2020-04-07 | 2021-10-14 | Personal Genome Diagnostics Inc. | Codes à barres flottants |
| WO2021232023A2 (fr) * | 2020-05-15 | 2021-11-18 | Swift Biosciences, Inc. | Procédés de pcr couplée à une ligature |
| CN112941147A (zh) * | 2021-03-02 | 2021-06-11 | 深圳市睿法生物科技有限公司 | 一种高保真靶标基因建库方法及其试剂盒 |
| CN113005121A (zh) * | 2021-04-25 | 2021-06-22 | 纳昂达(南京)生物科技有限公司 | 接头元件、试剂盒及其相关应用 |
Non-Patent Citations (2)
| Title |
|---|
| ILLUMINA: "Illumina Adapter Sequences", 1 October 2015 (2015-10-01), XP093139720, Retrieved from the Internet <URL:https://dnatech.genomecenter.ucdavis.edu/wp-content/uploads/2013/06/illumina-adapter-sequences_1000000002694-00.pdf> [retrieved on 20240311] * |
| See also references of WO2023082305A1 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN113999893B (zh) | 2022-11-01 |
| EP4202058A1 (fr) | 2023-06-28 |
| CN113999893A (zh) | 2022-02-01 |
| WO2023082305A1 (fr) | 2023-05-19 |
| US20240279647A1 (en) | 2024-08-22 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: UNKNOWN |
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| STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE INTERNATIONAL PUBLICATION HAS BEEN MADE |
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| PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
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| STAA | Information on the status of an ep patent application or granted ep patent |
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| 17P | Request for examination filed |
Effective date: 20230131 |
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| AK | Designated contracting states |
Kind code of ref document: A1 Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR |
|
| A4 | Supplementary search report drawn up and despatched |
Effective date: 20240404 |
|
| RIC1 | Information provided on ipc code assigned before grant |
Ipc: C12Q 1/6806 20180101ALI20240327BHEP Ipc: C12N 15/10 20060101ALI20240327BHEP Ipc: C40B 50/06 20060101ALI20240327BHEP Ipc: C12Q 1/6869 20180101AFI20240327BHEP |
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| DAV | Request for validation of the european patent (deleted) | ||
| DAX | Request for extension of the european patent (deleted) |