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CN110114472A - 将线性测序文库转换为环状测序文库的方法 - Google Patents

将线性测序文库转换为环状测序文库的方法 Download PDF

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Publication number
CN110114472A
CN110114472A CN201680091797.8A CN201680091797A CN110114472A CN 110114472 A CN110114472 A CN 110114472A CN 201680091797 A CN201680091797 A CN 201680091797A CN 110114472 A CN110114472 A CN 110114472A
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sequencing library
linear
primer
circular
converted
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周清
王磊
邱思
李贵波
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MGI Tech Co Ltd
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MGI Tech Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms

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  • Chemical & Material Sciences (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
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  • Wood Science & Technology (AREA)
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  • General Chemical & Material Sciences (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
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  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

提供了将线性测序文库转换为环状测序文库的方法。其中，该方法包括以下步骤：利用正向接头引物和反向接头引物将线性测序文库进行PCR扩增，以便获得扩增产物；将所述扩增产物进行热变性处理，以便获得单链DNA混合物；将所述单链DNA混合物进行环化处理，以便获得环状DNA产物，所述环状DNA产物构成所述环状测序文库，其中，正向接头引物与所述线性测序文库的正向接头的至少部分序列互补配对，反向接头引物与所述线性测序文库的反向接头的至少部分序列互补配对，正向接头引物和所述反向接头引物的至少之一的5’端核苷酸经过磷酸化修饰。

Description

PCT国内申请，说明书已公开。

Claims (11)

  1. PCT国内申请，权利要求书已公开。
CN201680091797.8A 2016-12-21 2016-12-21 将线性测序文库转换为环状测序文库的方法 Pending CN110114472A (zh)

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PCT/CN2016/111365 WO2018112806A1 (zh) 2016-12-21 2016-12-21 将线性测序文库转换为环状测序文库的方法

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CN114540473A (zh) * 2021-08-27 2022-05-27 四川大学华西第二医院 一种新型核酸测序系统
WO2022222101A1 (zh) * 2021-04-22 2022-10-27 深圳华大生命科学研究院 Rna测序文库的构建方法、测序方法及试剂盒
WO2023082305A1 (zh) * 2021-11-09 2023-05-19 纳昂达(南京)生物科技有限公司 兼容双测序平台的建库元件、试剂盒及建库方法
CN117551645A (zh) * 2023-10-12 2024-02-13 江苏先声医学诊断有限公司 一种将Illumina文库快速转化为MGI测序平台文库的试剂及方法
WO2024119413A1 (zh) * 2022-12-07 2024-06-13 深圳华大智造科技股份有限公司 基于一步法的双核酸纳米球制备方法

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CN111315895A (zh) * 2017-09-14 2020-06-19 豪夫迈·罗氏有限公司 用于产生环状单链dna文库的新型方法
CN110760936B (zh) * 2018-07-26 2023-04-28 深圳华大生命科学研究院 构建dna甲基化文库的方法及其应用
CA3122127C (en) 2018-12-05 2023-05-16 Egi Tech (Shen Zhen) Co, Limited Rolling circle amplification method, method for preparing sequencing library, and dna nanosphere prepared therefrom
KR102872602B1 (ko) * 2019-06-26 2025-10-16 엠쥐아이 테크 컴퍼니 엘티디. 네스티드 다중 pcr 고처리량 시퀀싱 라이브러리의 제조 방법 및 키트
CN113646471A (zh) * 2019-09-20 2021-11-12 武汉华大医学检验所有限公司 测序文库的构建方法、测序方法及试剂盒和应用
CN113046835A (zh) * 2019-12-27 2021-06-29 深圳华大生命科学研究院 检测慢病毒插入位点的测序文库构建方法和慢病毒插入位点检测方法
CN115198003B (zh) * 2020-11-18 2023-07-25 鲲羽生物科技(江门)有限公司 适用于barcode测序的转录组空间位置信息的检测方法及其应用
CN113862261B (zh) * 2021-11-15 2022-11-15 纳昂达(南京)生物科技有限公司 一组应用于不同高通量测序平台文库的通用环化陪伴序列、其试剂盒以及环化方法
CN118414425A (zh) * 2022-01-20 2024-07-30 深圳华大智造科技股份有限公司 一种接头及其在构建dnb文库中的应用

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022222101A1 (zh) * 2021-04-22 2022-10-27 深圳华大生命科学研究院 Rna测序文库的构建方法、测序方法及试剂盒
CN114540473A (zh) * 2021-08-27 2022-05-27 四川大学华西第二医院 一种新型核酸测序系统
CN114540473B (zh) * 2021-08-27 2024-03-01 四川大学华西第二医院 一种新型核酸测序系统
WO2023082305A1 (zh) * 2021-11-09 2023-05-19 纳昂达(南京)生物科技有限公司 兼容双测序平台的建库元件、试剂盒及建库方法
EP4202058A4 (en) * 2021-11-09 2024-05-01 Nanodigmbio (Nanjing) Biotechnology Co., Ltd BANK BUILDING ELEMENT COMPATIBLE WITH DOUBLE SEQUENCING PLATFORMS, BANK BUILDING KIT AND METHOD
WO2024119413A1 (zh) * 2022-12-07 2024-06-13 深圳华大智造科技股份有限公司 基于一步法的双核酸纳米球制备方法
CN117551645A (zh) * 2023-10-12 2024-02-13 江苏先声医学诊断有限公司 一种将Illumina文库快速转化为MGI测序平台文库的试剂及方法

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Application publication date: 20190809