EP4162035A4 - Enzymes indépendantes d'une matrice modifiée pour la synthèse de polydésoxynucléotides - Google Patents
Enzymes indépendantes d'une matrice modifiée pour la synthèse de polydésoxynucléotidesInfo
- Publication number
- EP4162035A4 EP4162035A4 EP21818476.0A EP21818476A EP4162035A4 EP 4162035 A4 EP4162035 A4 EP 4162035A4 EP 21818476 A EP21818476 A EP 21818476A EP 4162035 A4 EP4162035 A4 EP 4162035A4
- Authority
- EP
- European Patent Office
- Prior art keywords
- polydeoxynucleotides
- synthesis
- matrix
- modified
- independent enzymes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1264—DNA nucleotidylexotransferase (2.7.7.31), i.e. terminal nucleotidyl transferase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/07—Nucleotidyltransferases (2.7.7)
- C12Y207/07031—DNA nucleotidylexotransferase (2.7.7.31), i.e. terminal deoxynucleotidyl transferase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Enzymes And Modification Thereof (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US16/891,449 US11390858B2 (en) | 2014-10-20 | 2020-06-03 | Modified template-independent enzymes for polydeoxynucleotide synthesis |
| PCT/US2021/035685 WO2021247851A2 (fr) | 2020-06-03 | 2021-06-03 | Enzymes indépendantes d'une matrice modifiée pour la synthèse de polydésoxynucléotides |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| EP4162035A2 EP4162035A2 (fr) | 2023-04-12 |
| EP4162035A4 true EP4162035A4 (fr) | 2025-03-12 |
Family
ID=78831733
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP21818476.0A Pending EP4162035A4 (fr) | 2020-06-03 | 2021-06-03 | Enzymes indépendantes d'une matrice modifiée pour la synthèse de polydésoxynucléotides |
Country Status (7)
| Country | Link |
|---|---|
| EP (1) | EP4162035A4 (fr) |
| JP (1) | JP2023528477A (fr) |
| KR (1) | KR20240016237A (fr) |
| CN (1) | CN116075593A (fr) |
| AU (1) | AU2021285906A1 (fr) |
| CA (1) | CA3185851A1 (fr) |
| WO (1) | WO2021247851A2 (fr) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114410602B (zh) * | 2022-01-28 | 2024-01-19 | 赛纳生物科技(北京)有限公司 | 一种末端脱氧核苷酸转移酶的突变体及其应用 |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016064880A1 (fr) * | 2014-10-20 | 2016-04-28 | Molecular Assemblies, Inc. | Enzymes indépendantes de la matrice modifiées pour la synthèse de polydésoxynucléotides |
| WO2020081985A1 (fr) * | 2018-10-19 | 2020-04-23 | Molecular Assemblies, Inc. | Enzymes indépendantes à matrice modifiée pour la synthèse de polydésoxynucléotides |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10215035A1 (de) * | 2002-04-05 | 2003-10-23 | Roche Diagnostics Gmbh | Rekombinante terminale Deoxynukleotidyltransferase mit verbesserter Funktionalität |
| CN103667323B (zh) * | 2007-03-12 | 2016-02-03 | 丹尼斯科美国公司 | 经修饰的蛋白酶 |
| GB201502152D0 (en) * | 2015-02-10 | 2015-03-25 | Nuclera Nucleics Ltd | Novel use |
| FR3052462A1 (fr) * | 2016-06-14 | 2017-12-15 | Dna Script | Variants d'une adn polymerase de la famille polx |
| AU2019205606A1 (en) * | 2018-01-08 | 2020-07-30 | Centre National De La Recherche Scientifique | Variants of Terminal deoxynucleotidyl Transferase and uses thereof |
-
2021
- 2021-06-03 JP JP2022574577A patent/JP2023528477A/ja active Pending
- 2021-06-03 AU AU2021285906A patent/AU2021285906A1/en active Pending
- 2021-06-03 CN CN202180057936.6A patent/CN116075593A/zh active Pending
- 2021-06-03 WO PCT/US2021/035685 patent/WO2021247851A2/fr not_active Ceased
- 2021-06-03 CA CA3185851A patent/CA3185851A1/fr active Pending
- 2021-06-03 KR KR1020237000176A patent/KR20240016237A/ko active Pending
- 2021-06-03 EP EP21818476.0A patent/EP4162035A4/fr active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016064880A1 (fr) * | 2014-10-20 | 2016-04-28 | Molecular Assemblies, Inc. | Enzymes indépendantes de la matrice modifiées pour la synthèse de polydésoxynucléotides |
| WO2020081985A1 (fr) * | 2018-10-19 | 2020-04-23 | Molecular Assemblies, Inc. | Enzymes indépendantes à matrice modifiée pour la synthèse de polydésoxynucléotides |
Non-Patent Citations (1)
| Title |
|---|
| DATABASE UniProt [online] 1 November 1986 (1986-11-01), "DNA nucleotidylexotransferase (EC 2.7.7.31)", XP002812516, retrieved from EBI accession no. UNIPROT:P04053 Database accession no. P04053 * |
Also Published As
| Publication number | Publication date |
|---|---|
| AU2021285906A1 (en) | 2023-02-02 |
| WO2021247851A2 (fr) | 2021-12-09 |
| EP4162035A2 (fr) | 2023-04-12 |
| WO2021247851A3 (fr) | 2022-03-17 |
| CA3185851A1 (fr) | 2021-12-09 |
| JP2023528477A (ja) | 2023-07-04 |
| CN116075593A (zh) | 2023-05-05 |
| KR20240016237A (ko) | 2024-02-06 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE INTERNATIONAL PUBLICATION HAS BEEN MADE |
|
| PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
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| STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: REQUEST FOR EXAMINATION WAS MADE |
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| 17P | Request for examination filed |
Effective date: 20230102 |
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| AK | Designated contracting states |
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| DAV | Request for validation of the european patent (deleted) | ||
| DAX | Request for extension of the european patent (deleted) | ||
| RIC1 | Information provided on ipc code assigned before grant |
Ipc: C12Q 1/6806 20180101ALI20241114BHEP Ipc: C07H 21/04 20060101ALI20241114BHEP Ipc: C12P 19/34 20060101ALI20241114BHEP Ipc: C12N 15/70 20060101ALI20241114BHEP Ipc: C12N 15/10 20060101ALI20241114BHEP Ipc: C12N 9/12 20060101AFI20241114BHEP |
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| A4 | Supplementary search report drawn up and despatched |
Effective date: 20250211 |
|
| RIC1 | Information provided on ipc code assigned before grant |
Ipc: C12Q 1/6806 20180101ALI20250205BHEP Ipc: C07H 21/04 20060101ALI20250205BHEP Ipc: C12P 19/34 20060101ALI20250205BHEP Ipc: C12N 15/70 20060101ALI20250205BHEP Ipc: C12N 15/10 20060101ALI20250205BHEP Ipc: C12N 9/12 20060101AFI20250205BHEP |