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EP4162035A4 - MODIFIED, MATRIX-INDEPENDENT ENZYMES FOR THE SYNTHESIS OF POLYDEOXYNUCLEOTIDES - Google Patents

MODIFIED, MATRIX-INDEPENDENT ENZYMES FOR THE SYNTHESIS OF POLYDEOXYNUCLEOTIDES

Info

Publication number
EP4162035A4
EP4162035A4 EP21818476.0A EP21818476A EP4162035A4 EP 4162035 A4 EP4162035 A4 EP 4162035A4 EP 21818476 A EP21818476 A EP 21818476A EP 4162035 A4 EP4162035 A4 EP 4162035A4
Authority
EP
European Patent Office
Prior art keywords
polydeoxynucleotides
synthesis
matrix
modified
independent enzymes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP21818476.0A
Other languages
German (de)
French (fr)
Other versions
EP4162035A2 (en
Inventor
Julie L. TUBBS
Prem SINHA
Boguslaw STEC
Matthew T. HOLDEN
Christopher Wilson
J. William Efcavitch
Deanne W. SAMMOND
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Molecular Assemblies Inc
Original Assignee
Molecular Assemblies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US16/891,449 external-priority patent/US11390858B2/en
Application filed by Molecular Assemblies Inc filed Critical Molecular Assemblies Inc
Publication of EP4162035A2 publication Critical patent/EP4162035A2/en
Publication of EP4162035A4 publication Critical patent/EP4162035A4/en
Pending legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241Nucleotidyltransferases (2.7.7)
    • C12N9/1264DNA nucleotidylexotransferase (2.7.7.31), i.e. terminal nucleotidyl transferase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/26Preparation of nitrogen-containing carbohydrates
    • C12P19/28N-glycosides
    • C12P19/30Nucleotides
    • C12P19/34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y207/00Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07Nucleotidyltransferases (2.7.7)
    • C12Y207/07031DNA nucleotidylexotransferase (2.7.7.31), i.e. terminal deoxynucleotidyl transferase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP21818476.0A 2020-06-03 2021-06-03 MODIFIED, MATRIX-INDEPENDENT ENZYMES FOR THE SYNTHESIS OF POLYDEOXYNUCLEOTIDES Pending EP4162035A4 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US16/891,449 US11390858B2 (en) 2014-10-20 2020-06-03 Modified template-independent enzymes for polydeoxynucleotide synthesis
PCT/US2021/035685 WO2021247851A2 (en) 2020-06-03 2021-06-03 Modified template-independent enzymes for polydeoxynucleotide synthesis

Publications (2)

Publication Number Publication Date
EP4162035A2 EP4162035A2 (en) 2023-04-12
EP4162035A4 true EP4162035A4 (en) 2025-03-12

Family

ID=78831733

Family Applications (1)

Application Number Title Priority Date Filing Date
EP21818476.0A Pending EP4162035A4 (en) 2020-06-03 2021-06-03 MODIFIED, MATRIX-INDEPENDENT ENZYMES FOR THE SYNTHESIS OF POLYDEOXYNUCLEOTIDES

Country Status (7)

Country Link
EP (1) EP4162035A4 (en)
JP (1) JP2023528477A (en)
KR (1) KR20240016237A (en)
CN (1) CN116075593A (en)
AU (1) AU2021285906A1 (en)
CA (1) CA3185851A1 (en)
WO (1) WO2021247851A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114410602B (en) * 2022-01-28 2024-01-19 赛纳生物科技(北京)有限公司 Mutant of terminal deoxynucleotidyl transferase and application thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016064880A1 (en) * 2014-10-20 2016-04-28 Molecular Assemblies, Inc. Modified template-independent enzymes for polydeoxynucleotide systhesis
WO2020081985A1 (en) * 2018-10-19 2020-04-23 Molecular Assemblies, Inc. Modified template-independent enzymes for polydeoxynucleotide synthesis

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10215035A1 (en) * 2002-04-05 2003-10-23 Roche Diagnostics Gmbh Recombinant terminal deoxynucleotide transferase with improved functionality
CN103667323B (en) * 2007-03-12 2016-02-03 丹尼斯科美国公司 Modified proteolytic enzyme
GB201502152D0 (en) * 2015-02-10 2015-03-25 Nuclera Nucleics Ltd Novel use
FR3052462A1 (en) * 2016-06-14 2017-12-15 Dna Script POLYMERASE DNA VARIANTS OF THE POLX FAMILY
AU2019205606A1 (en) * 2018-01-08 2020-07-30 Centre National De La Recherche Scientifique Variants of Terminal deoxynucleotidyl Transferase and uses thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016064880A1 (en) * 2014-10-20 2016-04-28 Molecular Assemblies, Inc. Modified template-independent enzymes for polydeoxynucleotide systhesis
WO2020081985A1 (en) * 2018-10-19 2020-04-23 Molecular Assemblies, Inc. Modified template-independent enzymes for polydeoxynucleotide synthesis

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
DATABASE UniProt [online] 1 November 1986 (1986-11-01), "DNA nucleotidylexotransferase (EC 2.7.7.31)", XP002812516, retrieved from EBI accession no. UNIPROT:P04053 Database accession no. P04053 *

Also Published As

Publication number Publication date
AU2021285906A1 (en) 2023-02-02
WO2021247851A2 (en) 2021-12-09
EP4162035A2 (en) 2023-04-12
WO2021247851A3 (en) 2022-03-17
CA3185851A1 (en) 2021-12-09
JP2023528477A (en) 2023-07-04
CN116075593A (en) 2023-05-05
KR20240016237A (en) 2024-02-06

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RIC1 Information provided on ipc code assigned before grant

Ipc: C12Q 1/6806 20180101ALI20241114BHEP

Ipc: C07H 21/04 20060101ALI20241114BHEP

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