EP4010361A4 - Procédés et compositions pour la reconstitution de la microglie - Google Patents

Procédés et compositions pour la reconstitution de la microglie Download PDF

Info

Publication number: EP4010361A4
Authority: EP; European Patent Office
Prior art keywords: microglia; restoring; compositions; methods; restoring microglia
Prior art date: 2019-08-06
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Pending

Application number

EP20849655.4A

Other languages

German (de)

English (en)

Other versions

EP4010361A1 (fr

Inventor

Alessandra Biffi

Annita MONTEPELOSO

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Boston Childrens Hospital

Dana Farber Cancer Institute Inc

Original Assignee

Boston Childrens Hospital

Dana Farber Cancer Institute Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2019-08-06

Filing date

2020-08-06

Publication date

2023-08-09

2020-08-06 Application filed by Boston Childrens Hospital, Dana Farber Cancer Institute Inc filed Critical Boston Childrens Hospital

2022-06-15 Publication of EP4010361A1 publication Critical patent/EP4010361A1/fr

2023-08-09 Publication of EP4010361A4 publication Critical patent/EP4010361A4/fr

Status Pending legal-status Critical Current

Classifications

- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/715—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons
- C07K14/7158—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons for chemokines
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/21—Esters, e.g. nitroglycerine, selenocyanates
- A61K31/255—Esters, e.g. nitroglycerine, selenocyanates of sulfoxy acids or sulfur analogues thereof
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/30—Nerves; Brain; Eyes; Corneal cells; Cerebrospinal fluid; Neuronal stem cells; Neuronal precursor cells; Glial cells; Oligodendrocytes; Schwann cells; Astroglia; Astrocytes; Choroid plexus; Spinal cord tissue
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0618—Cells of the nervous system
- C12N5/0622—Glial cells, e.g. astrocytes, oligodendrocytes; Schwann cells
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/11—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from blood or immune system cells
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Engineering & Computer Science (AREA)
Biomedical Technology (AREA)
General Health & Medical Sciences (AREA)
Organic Chemistry (AREA)
Genetics & Genomics (AREA)
Medicinal Chemistry (AREA)
Zoology (AREA)
Cell Biology (AREA)
Pharmacology & Pharmacy (AREA)
Veterinary Medicine (AREA)
Public Health (AREA)
Animal Behavior & Ethology (AREA)
Biotechnology (AREA)
Bioinformatics & Cheminformatics (AREA)
Immunology (AREA)
Developmental Biology & Embryology (AREA)
Epidemiology (AREA)
Biochemistry (AREA)
Molecular Biology (AREA)
Neurosurgery (AREA)
Neurology (AREA)
Wood Science & Technology (AREA)
Biophysics (AREA)
General Engineering & Computer Science (AREA)
Gastroenterology & Hepatology (AREA)
Emergency Medicine (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Toxicology (AREA)
Chemical Kinetics & Catalysis (AREA)
General Chemical & Material Sciences (AREA)
Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
Virology (AREA)
Microbiology (AREA)
Hematology (AREA)
Plant Pathology (AREA)
Physics & Mathematics (AREA)
Ophthalmology & Optometry (AREA)

EP20849655.4A 2019-08-06 2020-08-06 Procédés et compositions pour la reconstitution de la microglie Pending EP4010361A4 (fr)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US201962883428P	2019-08-06	2019-08-06
PCT/US2020/045106 WO2021026294A1 (fr)	2019-08-06	2020-08-06	Procédés et compositions pour la reconstitution de la microglie

Publications (2)

Publication Number	Publication Date
EP4010361A1 EP4010361A1 (fr)	2022-06-15
EP4010361A4 true EP4010361A4 (fr)	2023-08-09

Family

ID=74502650

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
EP20849655.4A Pending EP4010361A4 (fr)	2019-08-06	2020-08-06	Procédés et compositions pour la reconstitution de la microglie

Country Status (4)

Country	Link
US (1)	US20220323503A1 (fr)
EP (1)	EP4010361A4 (fr)
CN (1)	CN114245803A (fr)
WO (1)	WO2021026294A1 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US12209948B2 (en) *	2020-06-26	2025-01-28	Cytek Biosciences, Inc.	Methods of forming multi-color fluorescence-based flow cytometry panel
GB2612467B (en)	2021-04-01	2025-11-19	Univ California	Genetic modification of mammalian cells to confer resistance to CSF1R antagonists
AU2022340720A1 (en) *	2021-08-30	2025-03-06	Carrygenes Bioengineering, Llc	Use of growth factors for t cell activation
WO2023220364A2 (fr) *	2022-05-13	2023-11-16	The Children's Medical Center Corporation	Méthodes et compositions améliorées pour la distribution de transgène et/ou la reconstitution de microglie
EP4549556A1 (fr)	2023-10-31	2025-05-07	Vilnius University	Procédé de génération de cellules microgliales knock-out du récepteur d'endocannabinoïde

Citations (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
WO2018071898A1 (fr) *	2016-10-14	2018-04-19	Children's Medical Center Corporation	Compositions et procédés pour le traitement de maladies ou de troubles du système nerveux central

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US20120192298A1 (en) *	2009-07-24	2012-07-26	Sigma Aldrich Co. Llc	Method for genome editing
SG10201912901YA (en) *	2013-04-17	2020-02-27	Genzyme Corp	Compositions and methods for treating and preventing macular degeneration
CN110114461A (zh) *	2016-08-17	2019-08-09	博德研究所	新型crispr酶和系统
AU2018329741B2 (en) *	2017-09-08	2025-02-20	Poseida Therapeutics, Inc.	Compositions and methods for chimeric ligand receptor (CLR)-mediated conditional gene expression

2020
- 2020-08-06 CN CN202080055641.0A patent/CN114245803A/zh active Pending
- 2020-08-06 WO PCT/US2020/045106 patent/WO2021026294A1/fr not_active Ceased
- 2020-08-06 EP EP20849655.4A patent/EP4010361A4/fr active Pending
- 2020-08-06 US US17/632,752 patent/US20220323503A1/en active Pending

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
WO2018071898A1 (fr) *	2016-10-14	2018-04-19	Children's Medical Center Corporation	Compositions et procédés pour le traitement de maladies ou de troubles du système nerveux central

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
ASHEUER M ET AL: "Human CD34+ cells differentiate into microglia and express recombinant therapeutic protein", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, vol. 101, no. 10, 9 March 2004 (2004-03-09), pages 3557 - 3562, XP002370121, ISSN: 0027-8424, DOI: 10.1073/PNAS.0306431101 *
CAPOTONDO ALESSIA ET AL: "Intracerebroventricular delivery of hematopoietic progenitors results in rapid and robust engraftment of microglia-like cells", SCIENCE ADVANCES, vol. 3, no. 12, 1 December 2017 (2017-12-01), XP093058259, Retrieved from the Internet <URL:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5721728/pdf/1701211.pdf> [retrieved on 20230628], DOI: 10.1126/sciadv.1701211 *
CORAL-ANN B LEWIS: "Thesis", 1 January 2003 (2003-01-01), pages 212pp, XP009187651, Retrieved from the Internet <URL:http://summit.sfu.ca/item/11818> *
D. N. COOK ET AL: "Generation and Analysis of Mice Lacking the Chemokine Fractalkine", MOLECULAR AND CELLULAR BIOLOGY, vol. 21, no. 9, 1 May 2001 (2001-05-01), US, pages 3159 - 3165, XP055239285, ISSN: 0270-7306, DOI: 10.1128/MCB.21.9.3159-3165.2001 *
FUHRMANN MARTIN ET AL: "Microglial Cx3cr1 knockout prevents neuron loss in a mouse model of Alzheimer's disease", NATURE NEUROSCIENCE, vol. 13, no. 4, 1 April 2010 (2010-04-01), New York, pages 411 - 413, XP093058258, ISSN: 1097-6256, DOI: 10.1038/nn.2511 *
JUNG S ET AL: "Analysis of fractalkine receptor CX(3)CR1 function by targeted deletion and green fluorescent protein reporter gene insertion", MOLECULAR AND CELLULAR BIOLOGY, AMERICAN SOCIETY FOR PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS, US, vol. 20, no. 11, 1 June 2000 (2000-06-01), pages 4106 - 4114, XP002272343, ISSN: 0270-7306, DOI: 10.1128/MCB.20.11.4106-4114.2000 *
LEE SUNGHO ET AL: "CX3CR1 Deficiency Alters Microglial Activation and Reduces Beta-Amyloid Deposition in Two Alzheimer's Disease Mouse Models", THE AMERICAN JOURNAL OF PATHOLOGY, vol. 177, no. 5, 1 November 2010 (2010-11-01), US, pages 2549 - 2562, XP093058368, ISSN: 0002-9440, DOI: 10.2353/ajpath.2010.100265 *
See also references of WO2021026294A1 *

Also Published As

Publication number	Publication date
US20220323503A1 (en)	2022-10-13
EP4010361A1 (fr)	2022-06-15
WO2021026294A1 (fr)	2021-02-11
CN114245803A (zh)	2022-03-25

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Legal Events

Date	Code	Title	Description
2021-02-12	STAA	Information on the status of an ep patent application or granted ep patent	Free format text: STATUS: THE INTERNATIONAL PUBLICATION HAS BEEN MADE
2022-05-13	PUAI	Public reference made under article 153(3) epc to a published international application that has entered the european phase	Free format text: ORIGINAL CODE: 0009012
2022-05-13	STAA	Information on the status of an ep patent application or granted ep patent	Free format text: STATUS: REQUEST FOR EXAMINATION WAS MADE
2022-06-15	17P	Request for examination filed	Effective date: 20220304
2022-06-15	AK	Designated contracting states	Kind code of ref document: A1 Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
2022-11-09	DAV	Request for validation of the european patent (deleted)
2022-11-09	DAX	Request for extension of the european patent (deleted)
2023-08-09	A4	Supplementary search report drawn up and despatched	Effective date: 20230710
2023-08-09	RIC1	Information provided on ipc code assigned before grant	Ipc: C12N 5/079 20100101ALI20230704BHEP Ipc: A61P 25/00 20060101ALI20230704BHEP Ipc: A61K 38/00 20060101ALI20230704BHEP Ipc: A61K 35/30 20150101ALI20230704BHEP Ipc: A61K 31/255 20060101ALI20230704BHEP Ipc: C12N 15/90 20060101ALI20230704BHEP Ipc: C12N 15/10 20060101ALI20230704BHEP Ipc: C07K 14/715 20060101AFI20230704BHEP