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EP2001871A4 - Agents réactifs utiles pour synthétiser des oligonucléotides marqués à la rhodamine - Google Patents

Agents réactifs utiles pour synthétiser des oligonucléotides marqués à la rhodamine

Info

Publication number
EP2001871A4
EP2001871A4 EP07759970A EP07759970A EP2001871A4 EP 2001871 A4 EP2001871 A4 EP 2001871A4 EP 07759970 A EP07759970 A EP 07759970A EP 07759970 A EP07759970 A EP 07759970A EP 2001871 A4 EP2001871 A4 EP 2001871A4
Authority
EP
European Patent Office
Prior art keywords
rhodamine
marketed
agents useful
synthesizing oligonucleotides
reactive agents
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP07759970A
Other languages
German (de)
English (en)
Other versions
EP2001871A2 (fr
EP2001871B1 (fr
Inventor
Scott C Benson
Ruiming N Zou
Krishna G Upadhya
Paul M Kenney
Jonathan M Cassel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Applied Biosystems LLC
Original Assignee
Applied Biosystems Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Applied Biosystems Inc filed Critical Applied Biosystems Inc
Publication of EP2001871A2 publication Critical patent/EP2001871A2/fr
Publication of EP2001871A4 publication Critical patent/EP2001871A4/fr
Application granted granted Critical
Publication of EP2001871B1 publication Critical patent/EP2001871B1/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
    • C07F9/02—Phosphorus compounds
    • C07F9/547—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom
    • C07F9/6561—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom containing systems of two or more relevant hetero rings condensed among themselves or condensed with a common carbocyclic ring or ring system, with or without other non-condensed hetero rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
    • C07D311/96—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings spiro-condensed with carbocyclic rings or ring systems
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
    • C07F9/02—Phosphorus compounds
    • C07F9/06—Phosphorus compounds without P—C bonds
    • C07F9/22—Amides of acids of phosphorus
    • C07F9/24—Esteramides
    • C07F9/2404—Esteramides the ester moiety containing a substituent or a structure which is considered as characteristic
    • C07F9/2408—Esteramides the ester moiety containing a substituent or a structure which is considered as characteristic of hydroxyalkyl compounds
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
    • C07F9/02—Phosphorus compounds
    • C07F9/06—Phosphorus compounds without P—C bonds
    • C07F9/22—Amides of acids of phosphorus
    • C07F9/24—Esteramides
    • C07F9/2404—Esteramides the ester moiety containing a substituent or a structure which is considered as characteristic
    • C07F9/242—Esteramides the ester moiety containing a substituent or a structure which is considered as characteristic of hydroxyaryl compounds
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
    • C07F9/02—Phosphorus compounds
    • C07F9/547—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom
    • C07F9/645—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having two nitrogen atoms as the only ring hetero atoms
    • C07F9/6509—Six-membered rings
    • C07F9/650952—Six-membered rings having the nitrogen atoms in the positions 1 and 4
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
    • C07F9/02—Phosphorus compounds
    • C07F9/547—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom
    • C07F9/6527—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having nitrogen and oxygen atoms as the only ring hetero atoms
    • C07F9/653—Five-membered rings
    • C07F9/65306—Five-membered rings containing two nitrogen atoms
    • C07F9/65312—Five-membered rings containing two nitrogen atoms having the two nitrogen atoms in positions 1 and 2
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
    • C07F9/02—Phosphorus compounds
    • C07F9/547—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom
    • C07F9/655—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having oxygen atoms, with or without sulfur, selenium, or tellurium atoms, as the only ring hetero atoms
    • C07F9/65515—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having oxygen atoms, with or without sulfur, selenium, or tellurium atoms, as the only ring hetero atoms the oxygen atom being part of a five-membered ring
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B11/00—Diaryl- or thriarylmethane dyes
    • C09B11/04—Diaryl- or thriarylmethane dyes derived from triarylmethanes, i.e. central C-atom is substituted by amino, cyano, alkyl
    • C09B11/10—Amino derivatives of triarylmethanes
    • C09B11/24—Phthaleins containing amino groups ; Phthalanes; Fluoranes; Phthalides; Rhodamine dyes; Phthaleins having heterocyclic aryl rings; Lactone or lactame forms of triarylmethane dyes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
EP07759970.2A 2006-03-31 2007-04-02 Oligonucléotides marqués à la rhodamine Active EP2001871B1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US78777706P 2006-03-31 2006-03-31
PCT/US2007/065798 WO2007115265A2 (fr) 2006-03-31 2007-04-02 Agents réactifs utiles pour synthétiser des oligonucléotides marqués à la rhodamine

Publications (3)

Publication Number Publication Date
EP2001871A2 EP2001871A2 (fr) 2008-12-17
EP2001871A4 true EP2001871A4 (fr) 2010-10-20
EP2001871B1 EP2001871B1 (fr) 2014-07-16

Family

ID=38564295

Family Applications (1)

Application Number Title Priority Date Filing Date
EP07759970.2A Active EP2001871B1 (fr) 2006-03-31 2007-04-02 Oligonucléotides marqués à la rhodamine

Country Status (7)

Country Link
US (5) US9040674B2 (fr)
EP (1) EP2001871B1 (fr)
JP (3) JP2009533022A (fr)
CN (1) CN101454315B (fr)
AU (1) AU2007234451A1 (fr)
CA (1) CA2647827A1 (fr)
WO (1) WO2007115265A2 (fr)

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RU2015151857A (ru) 2008-12-02 2019-01-15 Уэйв Лайф Сайенсес Джапан, Инк. Способ синтеза модифицированных по атому фосфора нуклеиновых кислот
MX342945B (es) 2009-07-06 2016-10-18 Ontorii Inc * Profármacos de ácido nucleico novedosos y métodos de uso de los mismos.
US10428019B2 (en) 2010-09-24 2019-10-01 Wave Life Sciences Ltd. Chiral auxiliaries
EP2658998B1 (fr) 2010-12-29 2015-04-22 Life Technologies Corporation Composés ddao en tant qu'étalons de référence fluorescents
EP3248982A1 (fr) 2011-07-19 2017-11-29 Wave Life Sciences Ltd. Reactifs de type thiosulfonate pour la synthèse d'acides nucléiques fonctionnalisés
WO2013134686A1 (fr) * 2012-03-09 2013-09-12 Promega Corporation Détecteurs de ph
CA2879023C (fr) 2012-07-13 2017-03-28 Wave Life Sciences Japan Groupe auxiliaire asymetrique
CN104661664B (zh) 2012-07-13 2020-07-03 波涛生命科学有限公司 手性控制
CA2879066C (fr) 2012-07-13 2019-08-13 Shin Nippon Biomedical Laboratories, Ltd. Adjuvant d'acide nucleique chiral
CN103122379A (zh) * 2012-12-24 2013-05-29 深圳先进技术研究院 基因转染实时监测的方法
CN103232489B (zh) * 2013-02-18 2016-09-28 常州津坛生物科技有限公司 一种荧光探针化合物及其制备方法和用途
WO2014135223A1 (fr) * 2013-03-08 2014-09-12 Illumina Cambridge Ltd Composés de rhodamine et leur utilisation comme marqueurs fluorescents
US10322173B2 (en) 2014-01-15 2019-06-18 Shin Nippon Biomedical Laboratories, Ltd. Chiral nucleic acid adjuvant having anti-allergic activity, and anti-allergic agent
JPWO2015108048A1 (ja) 2014-01-15 2017-03-23 株式会社新日本科学 抗腫瘍作用を有するキラル核酸アジュバンド及び抗腫瘍剤
US10144933B2 (en) 2014-01-15 2018-12-04 Shin Nippon Biomedical Laboratories, Ltd. Chiral nucleic acid adjuvant having immunity induction activity, and immunity induction activator
MX2016009290A (es) 2014-01-16 2017-02-28 Wave Life Sciences Ltd Diseño quiral.
CN106281309B (zh) * 2016-08-03 2018-07-10 陕西师范大学 硼酸衍生物功能化荧光探针在检测5-羟甲基胞嘧啶中的应用
CN106350064B (zh) * 2016-08-30 2019-03-29 上海大学 利用微波加热法水热炭化造纸黑液制备水溶性荧光碳材料的方法
ES2967690T3 (es) * 2018-05-07 2024-05-03 Cepheid Colorantes de fosforamidita de sulforodamina
CN112566986A (zh) 2018-08-10 2021-03-26 生命技术公司 经硅取代的罗丹明染料和染料缀合物
JP2022514397A (ja) * 2018-12-20 2022-02-10 ライフ テクノロジーズ コーポレーション 変更されたローダミン染料及びバイオアッセイにおけるそれらの使用
CN115803333A (zh) 2020-07-02 2023-03-14 生命技术公司 三核苷酸帽类似物、其制备和用途
CN116323965A (zh) 2020-07-23 2023-06-23 生命技术公司 使用染料的用于生物分析的组合物、系统和方法
US20240060117A1 (en) 2020-07-23 2024-02-22 Life Technologies Corporation Energy transfer dye conjugates for use in biological assays
CN117897454A (zh) 2021-07-21 2024-04-16 生命技术公司 二苯并呫吨猝灭剂、用途和制备方法
CN116333006A (zh) * 2023-04-07 2023-06-27 苏州欧利生物医药科技有限公司 一种带有荧光标记物的寡核苷酸的固相合成方法

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WO2000075236A1 (fr) * 1999-06-03 2000-12-14 Pe Corporation (Ny) Composes experimentaux de rhodamine convenant comme marqueurs fluorescents
WO2006020947A2 (fr) * 2004-08-13 2006-02-23 Epoch Biosciences, Inc. Colorants et conjugues fluorescents phosphonate

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US4557862A (en) 1983-10-28 1985-12-10 University Patents, Inc. Rhodamine derivatives as fluorogenic substrates for proteinases
US4965349A (en) 1987-12-24 1990-10-23 Applied Biosystems, Inc. Method of synthesizing oligonucleotides labeled with ammonia-labile groups on solid phase supports
US5231191A (en) 1987-12-24 1993-07-27 Applied Biosystems, Inc. Rhodamine phosphoramidite compounds
US5451463A (en) * 1989-08-28 1995-09-19 Clontech Laboratories, Inc. Non-nucleoside 1,3-diol reagents for labeling synthetic oligonucleotides
US5366860A (en) * 1989-09-29 1994-11-22 Applied Biosystems, Inc. Spectrally resolvable rhodamine dyes for nucleic acid sequence determination
US6028190A (en) 1994-02-01 2000-02-22 The Regents Of The University Of California Probes labeled with energy transfer coupled dyes
US6020481A (en) * 1996-04-01 2000-02-01 The Perkin-Elmer Corporation Asymmetric benzoxanthene dyes
US6080852A (en) * 1996-06-27 2000-06-27 The Perkin-Elmer Corporation 4,7-dichlororhodamine dyes
US6583168B1 (en) * 1997-11-25 2003-06-24 Applera Corporation Sulfonated diarylrhodamine dyes
US6255476B1 (en) 1999-02-22 2001-07-03 Pe Corporation (Ny) Methods and compositions for synthesis of labelled oligonucleotides and analogs on solid-supports
US7183405B2 (en) 1999-06-25 2007-02-27 Syn Gen, Inc. Compositions and methods for labeling oligonucleotides
US6750357B1 (en) 1999-06-25 2004-06-15 Syngen, Inc. Rhodamine-based fluorophores useful as labeling reagents
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Publication number Priority date Publication date Assignee Title
WO2000075236A1 (fr) * 1999-06-03 2000-12-14 Pe Corporation (Ny) Composes experimentaux de rhodamine convenant comme marqueurs fluorescents
WO2006020947A2 (fr) * 2004-08-13 2006-02-23 Epoch Biosciences, Inc. Colorants et conjugues fluorescents phosphonate

Also Published As

Publication number Publication date
US20150232494A1 (en) 2015-08-20
US20080071070A1 (en) 2008-03-20
US9040674B2 (en) 2015-05-26
JP2013048635A (ja) 2013-03-14
WO2007115265A3 (fr) 2008-01-17
JP5698206B2 (ja) 2015-04-08
US9783560B2 (en) 2017-10-10
US11407773B2 (en) 2022-08-09
US20230096149A1 (en) 2023-03-30
WO2007115265A2 (fr) 2007-10-11
US20180057516A1 (en) 2018-03-01
JP2014079254A (ja) 2014-05-08
WO2007115265A9 (fr) 2007-11-29
JP2009533022A (ja) 2009-09-17
US20200270287A1 (en) 2020-08-27
AU2007234451A1 (en) 2007-10-11
EP2001871A2 (fr) 2008-12-17
CN101454315B (zh) 2016-08-17
EP2001871B1 (fr) 2014-07-16
CN101454315A (zh) 2009-06-10
CA2647827A1 (fr) 2007-10-11

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