[go: up one dir, main page]

EP1578951A4 - Adn polymerases a activite de detection analogique a base reduite - Google Patents

Adn polymerases a activite de detection analogique a base reduite

Info

Publication number
EP1578951A4
EP1578951A4 EP03809647A EP03809647A EP1578951A4 EP 1578951 A4 EP1578951 A4 EP 1578951A4 EP 03809647 A EP03809647 A EP 03809647A EP 03809647 A EP03809647 A EP 03809647A EP 1578951 A4 EP1578951 A4 EP 1578951A4
Authority
EP
European Patent Office
Prior art keywords
dna polymerases
analog detection
base analog
reduced base
detection activity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP03809647A
Other languages
German (de)
English (en)
Other versions
EP1578951A2 (fr
Inventor
Joseph A Sorge
Holly H Hogrefe
Madhushree Ghosh
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Stratagene California
Original Assignee
Stratagene California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US10/280,962 external-priority patent/US20040081965A1/en
Priority claimed from US10/298,680 external-priority patent/US20040091873A1/en
Priority claimed from US10/408,601 external-priority patent/US20040086890A1/en
Application filed by Stratagene California filed Critical Stratagene California
Publication of EP1578951A2 publication Critical patent/EP1578951A2/fr
Publication of EP1578951A4 publication Critical patent/EP1578951A4/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP03809647A 2002-10-25 2003-10-24 Adn polymerases a activite de detection analogique a base reduite Withdrawn EP1578951A4 (fr)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US10/280,962 US20040081965A1 (en) 2002-10-25 2002-10-25 DNA polymerases with reduced base analog detection activity
US280962 2002-10-25
US298680 2002-11-18
US10/298,680 US20040091873A1 (en) 2002-10-25 2002-11-18 DNA polymerases with reduced base analog detection activity
US10/408,601 US20040086890A1 (en) 2002-10-25 2003-04-07 DNA polymerases with reduced base analog detection activity
US408601 2003-04-07
PCT/US2003/033997 WO2004038007A2 (fr) 2002-10-25 2003-10-24 Adn polymerases a activite de detection analogique a base reduite

Publications (2)

Publication Number Publication Date
EP1578951A2 EP1578951A2 (fr) 2005-09-28
EP1578951A4 true EP1578951A4 (fr) 2005-11-16

Family

ID=46205001

Family Applications (1)

Application Number Title Priority Date Filing Date
EP03809647A Withdrawn EP1578951A4 (fr) 2002-10-25 2003-10-24 Adn polymerases a activite de detection analogique a base reduite

Country Status (5)

Country Link
EP (1) EP1578951A4 (fr)
JP (1) JP2006507012A (fr)
AU (1) AU2003301590A1 (fr)
CA (1) CA2503038A1 (fr)
WO (1) WO2004038007A2 (fr)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7960157B2 (en) 2002-12-20 2011-06-14 Agilent Technologies, Inc. DNA polymerase blends and uses thereof
KR100844358B1 (ko) * 2006-10-02 2008-07-07 한국해양연구원 돌연변이 ｄｎａ 중합효소들 및 그의 유전자들
JP5258512B2 (ja) * 2008-10-30 2013-08-07 株式会社日立製作所 エキソヌクレアーゼ活性増強ｄｎａポリメラーゼ変異体
CN102272319B (zh) * 2009-01-08 2014-08-27 伯乐实验室公司 用于提高核酸扩增反应效率的方法和组合物
JP6478445B2 (ja) * 2012-09-28 2019-03-06 東洋紡株式会社 改変された耐熱性ｄｎａポリメラーゼ
JP6493209B2 (ja) * 2013-08-06 2019-04-03 東洋紡株式会社 核酸増幅法
JP6428997B2 (ja) * 2013-08-06 2018-11-28 東洋紡株式会社 核酸増幅法
WO2016077795A1 (fr) * 2014-11-14 2016-05-19 Illumina, Inc. Polymérases
WO2016084879A1 (fr) * 2014-11-28 2016-06-02 東洋紡株式会社 Dispositif d'amplification d'acide nucléique
JP6950184B2 (ja) * 2014-12-25 2021-10-13 東洋紡株式会社 Ｐｃｒ方法
GB201802744D0 (en) 2018-02-20 2018-04-04 Longas Tech Pty Ltd Method
CN114891761B (zh) * 2022-03-25 2024-01-12 上海威高医疗技术发展有限公司 Tth DNA聚合酶突变体及其应用
CN116410952B (zh) * 2022-12-28 2024-10-29 郑州玛特瑞斯生物科技有限公司 突变型Taq DNA聚合酶、编码基因、重组表达载体、重组菌及其应用
CN117467640B (zh) * 2023-09-28 2024-12-13 郑州玛特瑞斯生物科技有限公司 突变型Taq DNA聚合酶及其应用
WO2025118178A1 (fr) * 2023-12-06 2025-06-12 深圳华大智造科技股份有限公司 Mutant d'adn polymérase et son utilisation

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001092501A1 (fr) * 2000-05-26 2001-12-06 Mj Bioworks, Inc. Amelioration d'enzymes modificatrices de l'acide nucleique
WO2003060144A2 (fr) * 2001-12-21 2003-07-24 Stratagene Compositions de polymerases d'adn de haute fidelite et utilisations leur convenant
WO2003089637A1 (fr) * 2002-04-17 2003-10-30 The University Of Newcastle_Upon Tyne Mutation d'adn polymerases extraites d'archeobacteries

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001032887A1 (fr) * 1999-10-29 2001-05-10 Stratagene Compositions et procedes utilisant des adn polymerases
AU1491401A (en) * 1999-11-23 2001-06-04 Amersham Pharmacia Biotech Inc. Improving dideoxynucleotide-triphosphate utilization by the hyper-thermophilic dna polymerase from the archaeon pyrococcus furiosus
JP2001269188A (ja) * 2000-03-11 2001-10-02 Roche Diagnostics Gmbh Ｐｃｒにおける性能が向上したｂ型ｄｎａポリメラーゼ変異体

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001092501A1 (fr) * 2000-05-26 2001-12-06 Mj Bioworks, Inc. Amelioration d'enzymes modificatrices de l'acide nucleique
WO2003060144A2 (fr) * 2001-12-21 2003-07-24 Stratagene Compositions de polymerases d'adn de haute fidelite et utilisations leur convenant
WO2003089637A1 (fr) * 2002-04-17 2003-10-30 The University Of Newcastle_Upon Tyne Mutation d'adn polymerases extraites d'archeobacteries

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CONNOLLY B A ET AL: "Uracil recognition by archaeal family B DNA polymerases.", BIOCHEMICAL SOCIETY TRANSACTIONS. ENGLAND JUN 2003, vol. 31, no. Pt 3, June 2003 (2003-06-01), pages 699 - 702, XP009015138, ISSN: 0300-5127 *
FOGG MARK J ET AL: "Structural basis for uracil recognition by archaeal family B DNA polymerases.", NATURE STRUCTURAL BIOLOGY. UNITED STATES DEC 2002, vol. 9, no. 12, December 2002 (2002-12-01), pages 922 - 927, XP002250141, ISSN: 1072-8368 *
GARDNER ANDREW F ET AL: "Determinants of nucleotide sugar recognition in an archaeon DNA polymerase", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 27, no. 12, 15 June 1999 (1999-06-15), pages 2545 - 2553, XP002163101, ISSN: 0305-1048 *

Also Published As

Publication number Publication date
CA2503038A1 (fr) 2004-05-06
AU2003301590A1 (en) 2004-05-13
WO2004038007A3 (fr) 2005-02-10
WO2004038007A2 (fr) 2004-05-06
JP2006507012A (ja) 2006-03-02
EP1578951A2 (fr) 2005-09-28

Similar Documents

Publication Publication Date Title
AU2003296896A8 (en) Dna polymerase mutants with reverse transcriptase activity
AU2003256703A8 (en) Biosensor arrays and methods
DE60222809D1 (en) Biosensor
EP1506310A4 (fr) Biocapteur
EP1578951A4 (fr) Adn polymerases a activite de detection analogique a base reduite
EP1731525A4 (fr) Derives nucleotidiques et puce adn
EP1652855A4 (fr) Polypeptides agissant sur des predispositions a des pathologies cerebrales et utilisation desdits polypeptides
AU2003234432A8 (en) Voice activity detection
IL174572A0 (en) Refractory period tracking and arrhythmia detection
GB0427709D0 (en) Waveform analysis
PT1633771E (pt) Conjugados de macrolidos com actividade anti-inflamatória
AU2003285489A8 (en) Nucleic acid ligands and uses therefor
IL176358A0 (en) 3-cyano-quinoline derivatives with antiproliferative activity
AU2003273778A8 (en) Oligonucleotide analogues for detecting and analyzing nucleic acids
AU2003290722A8 (en) Methods and compositions for detecting telomerase activity
AP2005003215A0 (en) Storing and detecting nucleic acid administered toa solid carrier.
AU2003254298A8 (en) Species specific dna detection
AU2003295458A8 (en) Nucleic acids and proteins with thioredoxin reductase activity
GB0208153D0 (en) Biosensor
AU2003293382A8 (en) Methods for identifying the activity of gene products
AU2003294287A8 (en) A continuous-read assay for the detection of de novo hcv rna polymerase activity
EP1590432A4 (fr) Composition d'adn et ses utilisations
GB2386099B (en) Support and dedicated pen assembly
AU2003268332A8 (en) Rna-cleaving dna enzymes with altered regio- or enantioselectivity
GB0218089D0 (en) Target DNA

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20050524

AK Designated contracting states

Kind code of ref document: A2

Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT RO SE SI SK TR

AX Request for extension of the european patent

Extension state: AL LT LV MK

RIC1 Information provided on ipc code assigned before grant

Ipc: 7C 07H 21/04 B

Ipc: 7C 12P 19/34 B

Ipc: 7C 12Q 1/68 B

Ipc: 7C 12N 9/00 B

Ipc: 7C 12N 9/12 A

A4 Supplementary search report drawn up and despatched

Effective date: 20050930

RIC1 Information provided on ipc code assigned before grant

Ipc: 7C 12N 15/03 B

Ipc: 7C 12N 9/12 A

DAX Request for extension of the european patent (deleted)
RIN1 Information on inventor provided before grant (corrected)

Inventor name: GHOSH, MADHUSHREE

Inventor name: HOGREFE, HOLLY, H.

Inventor name: SORGE, JOSEPH, A.

17Q First examination report despatched

Effective date: 20081229

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 20110729