EP1578951A4 - Dna polymerases with reduced base analog detection activity - Google Patents

Dna polymerases with reduced base analog detection activity

Info

Publication number: EP1578951A4
Authority: EP; European Patent Office
Prior art keywords: dna polymerases; analog detection; base analog; reduced base; detection activity
Prior art date: 2002-10-25
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Withdrawn

Application number

EP03809647A

Other languages

German (de)

French (fr)

Other versions

EP1578951A2 (en

Inventor

Joseph A Sorge

Holly H Hogrefe

Madhushree Ghosh

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Stratagene California

Original Assignee

Stratagene California

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2002-10-25

Filing date

2003-10-24

Publication date

2005-11-16

2002-10-25 Priority claimed from US10/280,962 external-priority patent/US20040081965A1/en

2002-11-18 Priority claimed from US10/298,680 external-priority patent/US20040091873A1/en

2003-04-07 Priority claimed from US10/408,601 external-priority patent/US20040086890A1/en

2003-10-24 Application filed by Stratagene California filed Critical Stratagene California

2005-09-28 Publication of EP1578951A2 publication Critical patent/EP1578951A2/en

2005-11-16 Publication of EP1578951A4 publication Critical patent/EP1578951A4/en

Status Withdrawn legal-status Critical Current

Classifications

- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing

Landscapes

Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Organic Chemistry (AREA)
Molecular Biology (AREA)
Engineering & Computer Science (AREA)
Genetics & Genomics (AREA)
Biochemistry (AREA)
Wood Science & Technology (AREA)
Zoology (AREA)
Biotechnology (AREA)
General Health & Medical Sciences (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
Microbiology (AREA)
Biomedical Technology (AREA)
Medicinal Chemistry (AREA)
Physics & Mathematics (AREA)
Analytical Chemistry (AREA)
Biophysics (AREA)
Immunology (AREA)
Enzymes And Modification Thereof (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

EP03809647A 2002-10-25 2003-10-24 Dna polymerases with reduced base analog detection activity Withdrawn EP1578951A4 (en)

Applications Claiming Priority (7)

Application Number	Priority Date	Filing Date	Title
US408601		1982-08-16
US10/280,962 US20040081965A1 (en)	2002-10-25	2002-10-25	DNA polymerases with reduced base analog detection activity
US280962		2002-10-25
US298680		2002-11-18
US10/298,680 US20040091873A1 (en)	2002-10-25	2002-11-18	DNA polymerases with reduced base analog detection activity
US10/408,601 US20040086890A1 (en)	2002-10-25	2003-04-07	DNA polymerases with reduced base analog detection activity
PCT/US2003/033997 WO2004038007A2 (en)	2002-10-25	2003-10-24	Dna polymerases with reduced base analog detection activity

Publications (2)

Publication Number	Publication Date
EP1578951A2 EP1578951A2 (en)	2005-09-28
EP1578951A4 true EP1578951A4 (en)	2005-11-16

Family

ID=46205001

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
EP03809647A Withdrawn EP1578951A4 (en)	2002-10-25	2003-10-24	Dna polymerases with reduced base analog detection activity

Country Status (5)

Country	Link
EP (1)	EP1578951A4 (en)
JP (1)	JP2006507012A (en)
AU (1)	AU2003301590A1 (en)
CA (1)	CA2503038A1 (en)
WO (1)	WO2004038007A2 (en)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US7960157B2 (en)	2002-12-20	2011-06-14	Agilent Technologies, Inc.	DNA polymerase blends and uses thereof
KR100844358B1 (en) *	2006-10-02	2008-07-07	한국해양연구원	Mutant DNA polymerases and their genes
JP5258512B2 (en) *	2008-10-30	2013-08-07	株式会社日立製作所	DNA polymerase mutant with enhanced exonuclease activity
WO2010080910A1 (en) *	2009-01-08	2010-07-15	Bio-Rad Laboratories, Inc.	Methods and compositions for improving efficiency of nucleic acids amplification reactions
JP6478445B2 (en) *	2012-09-28	2019-03-06	東洋紡株式会社	Modified thermostable DNA polymerase
JP6428997B2 (en) *	2013-08-06	2018-11-28	東洋紡株式会社	Nucleic acid amplification method
WO2015019952A1 (en) *	2013-08-06	2015-02-12	東洋紡株式会社	Method of amplifying nucleic acid
US10329545B2 (en)	2014-11-14	2019-06-25	Illumina, Inc.	Polymerases
JP6968536B2 (en) *	2014-11-28	2021-11-17	東洋紡株式会社	Nucleic acid amplification reagent
WO2016104272A1 (en) *	2014-12-25	2016-06-30	東洋紡株式会社	Pcr method
GB201802744D0 (en) *	2018-02-20	2018-04-04	Longas Tech Pty Ltd	Method
CN114891761B (en) *	2022-03-25	2024-01-12	上海威高医疗技术发展有限公司	Tth DNA polymerase mutant and application thereof
CN116410952B (en) *	2022-12-28	2024-10-29	郑州玛特瑞斯生物科技有限公司	Mutant Taq DNA polymerase, coding gene, recombinant expression vector, recombinant bacterium and application thereof
CN117467640B (en) *	2023-09-28	2024-12-13	郑州玛特瑞斯生物科技有限公司	Mutant Taq DNA polymerase and its application
WO2025118178A1 (en) *	2023-12-06	2025-06-12	深圳华大智造科技股份有限公司	Dna polymerase mutant and use thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
WO2001092501A1 (en) *	2000-05-26	2001-12-06	Mj Bioworks, Inc.	Improved nucleic acid modifying enzymes
WO2003060144A2 (en) *	2001-12-21	2003-07-24	Stratagene	High fidelity dna polymerase compositions and uses therefor
WO2003089637A1 (en) *	2002-04-17	2003-10-30	The University Of Newcastle_Upon Tyne	Mutation of dna polymerases from archaeobacteria

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US6946273B1 (en) *	1999-10-29	2005-09-20	Stratagene California	Compositions and methods utilizing DNA polymerases
AU1491401A (en) *	1999-11-23	2001-06-04	Amersham Pharmacia Biotech Inc.	Improving dideoxynucleotide-triphosphate utilization by the hyper-thermophilic dna polymerase from the archaeon pyrococcus furiosus
JP2001269188A (en) *	2000-03-11	2001-10-02	Roche Diagnostics Gmbh	Dna polymerase b variant exhibiting enhanced performance in pcr

2003
- 2003-10-24 WO PCT/US2003/033997 patent/WO2004038007A2/en not_active Ceased
- 2003-10-24 EP EP03809647A patent/EP1578951A4/en not_active Withdrawn
- 2003-10-24 JP JP2005501697A patent/JP2006507012A/en active Pending
- 2003-10-24 CA CA002503038A patent/CA2503038A1/en not_active Abandoned
- 2003-10-24 AU AU2003301590A patent/AU2003301590A1/en not_active Abandoned

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
WO2001092501A1 (en) *	2000-05-26	2001-12-06	Mj Bioworks, Inc.	Improved nucleic acid modifying enzymes
WO2003060144A2 (en) *	2001-12-21	2003-07-24	Stratagene	High fidelity dna polymerase compositions and uses therefor
WO2003089637A1 (en) *	2002-04-17	2003-10-30	The University Of Newcastle_Upon Tyne	Mutation of dna polymerases from archaeobacteria

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CONNOLLY B A ET AL: "Uracil recognition by archaeal family B DNA polymerases.", BIOCHEMICAL SOCIETY TRANSACTIONS. ENGLAND JUN 2003, vol. 31, no. Pt 3, June 2003 (2003-06-01), pages 699 - 702, XP009015138, ISSN: 0300-5127 *
FOGG MARK J ET AL: "Structural basis for uracil recognition by archaeal family B DNA polymerases.", NATURE STRUCTURAL BIOLOGY. UNITED STATES DEC 2002, vol. 9, no. 12, December 2002 (2002-12-01), pages 922 - 927, XP002250141, ISSN: 1072-8368 *
GARDNER ANDREW F ET AL: "Determinants of nucleotide sugar recognition in an archaeon DNA polymerase", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 27, no. 12, 15 June 1999 (1999-06-15), pages 2545 - 2553, XP002163101, ISSN: 0305-1048 *

Also Published As

Publication number	Publication date
AU2003301590A1 (en)	2004-05-13
EP1578951A2 (en)	2005-09-28
CA2503038A1 (en)	2004-05-06
WO2004038007A3 (en)	2005-02-10
JP2006507012A (en)	2006-03-02
WO2004038007A2 (en)	2004-05-06

Publication	Publication Date	Title
AU2003296896A8 (en)	2004-05-25	Dna polymerase mutants with reverse transcriptase activity
AU2003256703A8 (en)	2004-02-09	Biosensor arrays and methods
EP1731525A4 (en)	2010-01-20	Nucleotide derivatives and dna microarray
EP1578951A4 (en)	2005-11-16	Dna polymerases with reduced base analog detection activity
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GB0427709D0 (en)	2005-01-19	Waveform analysis
ZA200508388B (en)	2006-07-26	Macrolide-Conjugates with anti-inflammatory activity
AU2003285489A8 (en)	2004-06-07	Nucleic acid ligands and uses therefor
IL176358A0 (en)	2006-10-05	3-cyano-quinoline derivatives with antiproliferative activity
AU2003273778A8 (en)	2004-05-04	Oligonucleotide analogues for detecting and analyzing nucleic acids
AU2003290722A8 (en)	2004-06-03	Methods and compositions for detecting telomerase activity
AP2005003215A0 (en)	2005-03-31	Storing and detecting nucleic acid administered toa solid carrier.
AU2003254298A8 (en)	2004-02-23	Species specific dna detection
AU2003295458A8 (en)	2004-06-03	Nucleic acids and proteins with thioredoxin reductase activity
GB0208153D0 (en)	2002-05-22	Biosensor
AU2003293382A8 (en)	2004-06-23	Methods for identifying the activity of gene products
AU2003294705A8 (en)	2004-06-07	Ntsm gene
AU2003294287A8 (en)	2009-07-30	A continuous-read assay for the detection of de novo hcv rna polymerase activity
EP1590432A4 (en)	2008-05-14	Dna compositon and uses thereof
GB2386099B (en)	2004-10-20	Support and dedicated pen assembly
AU2003268332A8 (en)	2004-04-30	Rna-cleaving dna enzymes with altered regio- or enantioselectivity
GB0202462D0 (en)	2002-03-20	Nucleic acid analysis
GB0223738D0 (en)	2002-11-20	Dna analysis
GB2398123B (en)	2005-03-02	Methods for measuring enzyme activity

Legal Events

Date	Code	Title	Description
2005-08-12	PUAI	Public reference made under article 153(3) epc to a published international application that has entered the european phase	Free format text: ORIGINAL CODE: 0009012
2005-09-28	17P	Request for examination filed	Effective date: 20050524
2005-09-28	AK	Designated contracting states	Kind code of ref document: A2 Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT RO SE SI SK TR
2005-09-28	AX	Request for extension of the european patent	Extension state: AL LT LV MK
2005-10-12	RIC1	Information provided on ipc code assigned before grant	Ipc: 7C 07H 21/04 B Ipc: 7C 12P 19/34 B Ipc: 7C 12Q 1/68 B Ipc: 7C 12N 9/00 B Ipc: 7C 12N 9/12 A
2005-11-16	A4	Supplementary search report drawn up and despatched	Effective date: 20050930
2005-11-16	RIC1	Information provided on ipc code assigned before grant	Ipc: 7C 12N 15/03 B Ipc: 7C 12N 9/12 A
2006-02-15	DAX	Request for extension of the european patent (deleted)
2007-01-10	RIN1	Information on inventor provided before grant (corrected)	Inventor name: GHOSH, MADHUSHREE Inventor name: HOGREFE, HOLLY, H. Inventor name: SORGE, JOSEPH, A.
2009-01-28	17Q	First examination report despatched	Effective date: 20081229
2011-12-23	STAA	Information on the status of an ep patent application or granted ep patent	Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN
2012-01-25	18D	Application deemed to be withdrawn	Effective date: 20110729