EP1185368B1 - Pipette avec ejecteur d'embouts - Google Patents
Pipette avec ejecteur d'embouts Download PDFInfo
- Publication number
- EP1185368B1 EP1185368B1 EP99917735A EP99917735A EP1185368B1 EP 1185368 B1 EP1185368 B1 EP 1185368B1 EP 99917735 A EP99917735 A EP 99917735A EP 99917735 A EP99917735 A EP 99917735A EP 1185368 B1 EP1185368 B1 EP 1185368B1
- Authority
- EP
- European Patent Office
- Prior art keywords
- cylinder
- ejection
- ejection member
- pipette
- tip
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000007788 liquid Substances 0.000 claims abstract description 16
- 230000000295 complement effect Effects 0.000 claims description 4
- 210000000078 claw Anatomy 0.000 claims description 3
- 238000006073 displacement reaction Methods 0.000 claims description 2
- 238000012986 modification Methods 0.000 claims 3
- 230000004048 modification Effects 0.000 claims 3
- 238000007599 discharging Methods 0.000 abstract 1
- 210000000056 organ Anatomy 0.000 description 8
- 230000008878 coupling Effects 0.000 description 6
- 238000010168 coupling process Methods 0.000 description 6
- 238000005859 coupling reaction Methods 0.000 description 6
- 230000000712 assembly Effects 0.000 description 1
- 238000000429 assembly Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 230000000994 depressogenic effect Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/0275—Interchangeable or disposable dispensing tips
- B01L3/0279—Interchangeable or disposable dispensing tips co-operating with positive ejection means
Definitions
- the invention relates to a pipette for transferring liquids comprising at least one minus a piston-cylinder assembly arranged so as to be provided with a transfer tip.
- Pipettes of this type are generally known, an example being described in US Patent 5,614,153.
- Such a pipette makes it possible to transfer small amounts of liquid in a replaceable tip mounted at the end free of the pipette cylinder. This transfer takes place without contamination of the pipette, the used tips being replaced by new tips.
- the pipette is equipped with a tip ejector that can be operated manually and comprises an ejection member sliding along the cylinder to abut against the tip and push it so as to separate it from the cylinder. In this pipette, the ejection organ travels an invariable distance and its final position is fixed.
- the tips used may come from different manufacturers or have different different shapes, so that their dimensions can vary from one to the other.
- the position of the tips on the cylinder may differ depending on the variants available on the market and according to the commitment force. It follows a variation of the position of the edge of the tip on which the organ abuts ejection. The variations of position being of the order of magnitude of the race of the ejection member, some tips are not ejected or can abut on the ejector before the creation of a seal between the tip and the cylinder of the pipette.
- US-A-4,965,050 discloses a pipette for the transfer of liquids relating to a tip ejector device on which an adapter ring is mounted so that when a piston actuator is fully depressed, an edge of the adapter ring comes into contact with a edge of a mouthpiece and ejects it.
- An object of the invention is to provide a pipette which allows to attach and to eject the tips in a reliable manner, in particular taking into account the variable position of the edge of the tip relative to the pipette.
- a pipette is preferably designed to be easy to use and robust.
- the present pipette for transferring liquids has at least one piston-cylinder assembly, the cylinder of which can be provided with a nozzle replaceable, a piston actuator to cause aspiration and the delivery of the liquid and a tip ejector device comprising a ejection member movable along the cylinder, has the particularity that the ejector device comprises a position adjustment device arranged way to change the limit position reached at the end of the race by the eytremity of the election member during an ejection operation of the mouthpiece by setting the starting position of a free edge of the ejection end or by a variation of the stroke of the ejection member relative to the cylinder.
- the adjustment will generally be through the adjustment of the starting position of the free edge of the organ ejection intended to abut on the upper edge of the tip.
- the present pipette makes it possible to attach and eject, reliably, tips of different shapes and sizes.
- the position adjusting device may comprise a socket arranged on the cylinder of the piston-cylinder assembly, the cooperation between this sleeve and the ejection member for adjusting the position of the end of the organ ejection with respect to the cylinder.
- the socket and the ejection member can be coupled by threaded parts or by the support of a pin or other element against a crown having an inclined surface, so that the adjustment of the position of the ejection member is effected by a relative rotation between the socket and this organ.
- the ejection member is rotatable and the socket is held fixed, and in one embodiment alternatively, the sleeve is rotatable and the ejection member is fixed.
- the socket and / or the ejection member may be provided with notches, a pin or means to define the angular position with respect to the counterpart.
- the socket which can be mounted on a part of the cylinder, and the ejection member are easily accessible and adjustable.
- Variations of the position adjustment device consist in using, for example, example, a socket whose position is adjustable relative to the organ of ejection and which is mounted on this member so as to form the end ejection of it.
- the socket and the ejection member may have parts respective threaded or other means of attachment to adjust the position of the socket according to the tip used.
- the ejection member can for example be coupled to its actuator through a variable length member so as to modify at least one limit position of the actuating member.
- FIG. 4 show, respectively, pipettes 1 and 1 ' whose like parts have been designated by the same reference numeral.
- a pipette comprises a pipette body 2 provided with a piston-cylinder assembly 3, 4, a piston actuator 5 and a device ejector 6, the piston actuator 5 comprises a pusher 7 acting on the piston 3 of the piston-cylinder assembly which makes it possible to suck and / or to discharge liquid through an exchangeable tip 8 mounted at the end 9 of the cylinder 4 of the piston-cylinder assembly.
- the cylinder 4 communicates with the tip 8 by an opening 10 of its end 9.
- the pusher 7 of the actuating device 5 of the piston comprises a button 11 and a rod 12.
- the rod 12 is coupled to the knob 11 via a threaded portion 13, so as to allow the adjustment of the position of the rod 12 and therefore of the piston 3 with respect to the cylinder to determine the volume of liquid aspirated.
- Other actuating devices of the piston 12 can be provided without departing from the scope of the invention, the actuation can be, for example, hydraulic or electric.
- the rod 12 is coupled at its end 14 in a separable manner to the piston 3.
- the piston 3 is provided with elastic arms 15 having depressions 56 on their surface interior so as to cooperate with the end 14 of the rod 12, which is ball-shaped.
- the coupling between the elastic arms 15 and the end 14 is therefore quickly separable by application of a certain traction force on the piston-cylinder assembly relative to the body 2. This allows, for example, example, to exchange the piston-cylinder assemblies with volumes different suction or shape and different dimensions to be comply with different standards, or exchange defective sets without have to replace the entire device.
- the connection between the elastic arms 15 and the ball 14 form a set of plug and prize advantageous because it is very quick and easy to disconnect and reconnect devices.
- the ejector device 6 comprises an ejection member 16 or 16 'having a tubular portion slidably disposed on the cylinder 4, and a actuating device 17 of this ejection member.
- the device actuator 17 comprises a pusher 18 and a rod 19 connecting the pusher to a coupling member 20 or 20 '.
- the coupling member makes a connection separable between the ejection member 16 or 16 'and the actuating device 17 by snapping, for example by the interaction of protuberances and grooves formed on complementary parts.
- the ejection member 16 or 16 ' is moved over a distance determined by the displacement of this pusher.
- the end 22 of the ejection member abuts on the upper edge 23 of the nozzle for to separate it from the cylinder 4.
- the pusher 18 is advantageously placed same side of the pipette body as the claw 21, which facilitates the actuation of the ejection pusher easily accessible to the operator.
- this provision makes the opposite side of the free pipette body to easily arrange a display device.
- the ejector device comprises a position adjusting device 24 comprising a threaded portion 25 to the upper end of the rod 19 which is engaged in a threaded portion complementary to a bore 26 of the pusher 18.
- the head of the threaded portion 25 is accessible from the outside to allow adjustment of the stem position 19 relative to the pusher 18, the coupling member 20 allowing the rotation of the rod 19.
- This rotation moves the ejection member 16 relative to the 4.
- the position of the end 22 of the ejection member 16 can therefore be adjusted to allow the ejection of tips 8 having upper edges located at different heights relative to the end 9 of the cylinder.
- the position of the pusher 18 is adjusted to vary the stroke of the actuating member determined by the distance between the limit position high of the pusher and the sleeve or claw 21.
- the variation of the stroke of the pusher 18 thus also modifies the limit position of the end 22 of the ejection member reached during the actuation of the ejector. We obtain reliable ejection of tips having positions and / or dimensions different.
- Figures 1 to 3 show a preferred embodiment of the invention.
- the position adjusting device 24 ' has a socket 27 mounted around the cylinder 4 near the upper end thereof.
- the bushing 27 has a peripheral groove 28 into which a protruding part of the coupling member 20 'connected to the actuating rod 19.
- the sleeve 27 can rotate about its axis, while being integral, in the axial direction, with the actuating device of the ejection member 16 '.
- An internal thread 29 of the sleeve 27 cooperates with a threading 30 on the upper part of the ejection member 16 ', so that rotation of the socket allows the end 22 to be moved away or the ejection member of the end 9 of the cylinder 4.
- the inner surface of the sleeve has a series of axial notches 31 in which a gadroon 32 the periphery of the upper part of the member 16 'can engage to define the angular position of the sleeve relative to this body.
- the member 16 ' is prevented from rotating around its axis and therefore moves in the axial direction relative to the cylinder 4 when adjusting its position by rotation of the sleeve 27.
- the sleeve 27 is fixed and the member 16 'is rotatably mounted so that rotation of the member of ejection makes it possible to move the end 22 of the end 9 away from cylinder 4.
- the ejector device has a 24 "position adjustment device having a fixed sleeve 27 'and an organ ejection nozzle 16 "mounted so as to allow its rotation on the cylinder 4.
- the organ ejection is provided with a piece 32 with a pin 32 "which engages in notches 31 'of a ring 29' having an inclined engagement surface 29 "The notches 31 'arranged at intervals around the periphery of the crown and thus define different positions in height for the adjustment the position of the end 22 of the ejection member 16 ". acting on the sleeve 27 'and on the member 16 "ensures the engagement of the pin 32" in one of the notches 31 '.
- the piston-cylinder device of FIG. 5 can also comprise a piston and means for enabling quick coupling to a pipette body similar to the means described above in connection with the embodiment of FIG. 2.
- a socket of adjustment can be mounted on an end portion of the ejection member, according to the variants shown in Figures 6 to 9, to form an ejection edge 22 'whose position can be adjusted relative to the cylinder 4 of the pipette.
- a bushing 33 or 33 ' is mounted on the part 34 or 34 'of the ejection member which has a thread 35 or 35' cooperating with a complementary thread of the sleeve.
- notch streaks 36 are formed on the bottom of the net to allow a good definition of the angular position of the socket 33.
- the notch is displaced on the cylindrical portion 37 of the ejection member and formed by a set of notches-gadroons 38 on this part and the socket 33.
- Fig. 7 shows a variant according to which a sleeve 39 is mounted on a end portion 40 of the ejection member via pins, such as 41, which are positioned in corresponding grooves arranged on the around the edge of the portion 40.
- These grooves, such as 42, 43, 44, have heights different that allow to define corresponding positions of the socket relative to the ejection member. The guiding of the socket is ensured by the cylinder 4 on which it slides.
- a sleeve 39 ' is provided with tabs longitudinal 50, provided at their free ends protuberances 51 arranged so as to be able to enter skylights, such as 52, 53, 54, located at different heights in the portion 40 'of the ejection member.
- the limit position of the end of the ejection member is adjustable at this end of to obtain effective ejection for tips whose edge superior is at different heights compared to the cylinder of the pipette.
Landscapes
- Health & Medical Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Devices For Use In Laboratory Experiments (AREA)
- Sampling And Sample Adjustment (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Feeding, Discharge, Calcimining, Fusing, And Gas-Generation Devices (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Description
Claims (13)
- Pipette pour le transfert de liquides comportant au moins un ensemble piston-cylindre (3, 4) dans lequel le cylindre (4) est agencé de façon à pouvoir être muni d'un embout (8) remplaçable, destiné à contenir le liquide à transférer, un dispositif d'actionnement de piston (5) pour effectuer l'aspiration et le refoulement de liquide, et un dispositif éjecteur d'embouts (6) ayant une extremité d'éjection, le dispositif éjecteur comportant un organe d'éjection (16, 16') déplaçable le long du cylindre pour éjecter un embout (8) de l'extrémité du cylindre, et un dispositif d'actionnement (17) de l'organe d'éjection, caractérisée en ce que le dispositif éjecteur (6) comporte un dispositif d'ajustement de position (24, 24', 24", 33, 33', 39, 39') agencé de façon à permettre de modifier la position limite d'un bord libre de l'extrémité d'éjection (22, 22') du dispositif éjecteur atteinte en fin de course par le réglage de la position de départ dudit bord libre.
- Pipette selon la revendication 1, caractérisée en ce que le dispositif d'ajustement de position (24,24',24") comporte une douille (27, 27', 33, 33', 39, 39') montée de façon à permettre un déplacement relatif entre une partie de l'organe d'éjection et ladite douille.
- Pipette selon la revendication 2, caractérisée en ce que la douille (27, 33, 33') et l'organe d'éjection comportent des parties filetées complémentaires (29, 30; 35, 35') coopérant pour permettre d'effectuer ledit déplacement relatif.
- Pipette selon la revendication 2, caractérisée en ce que le dispositif d'ajustement de position (24") comporte au moins un ergot (32") disposé sur l'organe d'éjection ou sur une contrepartie fixe et s'engageant dans des crans (31') disposés sur une surface inclinée (29") annulaire de la contrepartie fixe ou de l'organe d'éjection, respectivement.
- Pipette selon la revendication 4, caractérisée en ce que le dispositif d'ajustement de position comporte un ressort (55) appuyant sur l'organe d'éjection (16") pour assurer que l'ergot (32") s'engage dans un des crans (31').
- Pipette selon l'une des revendications 2 à 5, caractérisée en ce que la douille (27, 27', 33, 33') et l'organe d'éjection comportent un agencement (31, 32; 31', 32"; 36, 38) permettant de définir des positions angulaires relatives entre cet organe et la douille.
- Pipette selon l'une des revendications 2 à 6, caractérisée en ce que la douille (27) est disposée à proximité d'une extrémité (28) du cylindre (4) éloignée de l'extrémité (9) sur laquelle est placé l'embout (8).
- Pipette selon l'une des revendications précédentes, caractérisée en ce que le dispositif d'ajustement de position (24') comporte un agencement (20, 20') permettant une connexion séparable entre une tige d'actionnement d'éjection (19) et l'organe d'éjection (16, 16').
- Pipette selon la revendication 1, caractérisée en ce que le dispositif d'ajustement de position (24, 24') est agencé de façon à permettre de modifier la position de l'organe d'éjection (16, 16') ou d'une partie solidaire de celui-ci (33, 33', 39, 39') par rapport au cylindre (4) de la pipette.
- Pipette pour le transfert de liquides comportant au moins un ensemble piston-cylindre (3, 4) dans lequel le cylindre (4) est agencé de façon à pouvoir être muni d'un embout (8) remplaçable, destiné à contenir le liquide à transférer, un dispositif d'actionnement de piston (5) pour effectuer l'aspiration et le refoulement de liquide, et un dispositif éjecteur d'embouts (6) ayant une extremité d'éjection, le dispositif éjecteur comportant un organe d'éjection (16) déplaçable le long du cylindre pour éjecter un embout (8) d'e l'extrémité du cylindre, et un dispositif d'actionnement (17) de l'organe d'éjection, caractérisée en ce que le dispositif éjecteur (6) comporte un dispositif d'ajustement de position (24) agencé de façon, à permettre de modifier la position limite d'un bord libre de l'extrémité d'éjection (22) du dispositif éjecteur atteinte en fin de course par une variation de la course de l'organe d'éjection par rapport au cylindre.
- Pipette selon la revendication 9 ou la revendication 10, caractérisée en ce que le dispositif d'ajustement de position (24) comporte une tige d'actionnement (19) et un organe poussoir (18) couplés de façon à permettre de modifier la position de cette tige par rapport audit organe poussoir.
- Pipette selon l'une des revendications précédentes, caractérisée en ce que le dispositif éjecteur (6) comporte un poussoir (18) disposé du même côté du corps de pipette que la griffe (21), à proximité de celle-ci.
- Pipette selon l'une des revendications précédentes, caractérisée en ce que le dispositif d'actionnement (17) de l'organe d'éjection est connecté de manière séparable à l'organe d'éjection (16, 16') par des moyens d'encliquetage.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/CH1999/000210 WO2000069562A1 (fr) | 1999-05-18 | 1999-05-18 | Pipette avec ejecteur d'embouts |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| EP1185368A1 EP1185368A1 (fr) | 2002-03-13 |
| EP1185368B1 true EP1185368B1 (fr) | 2003-09-17 |
Family
ID=4551679
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP99917735A Expired - Lifetime EP1185368B1 (fr) | 1999-05-18 | 1999-05-18 | Pipette avec ejecteur d'embouts |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US6833114B1 (fr) |
| EP (1) | EP1185368B1 (fr) |
| AT (1) | ATE249885T1 (fr) |
| AU (1) | AU3593199A (fr) |
| DE (1) | DE69911474T2 (fr) |
| ES (1) | ES2207204T3 (fr) |
| WO (1) | WO2000069562A1 (fr) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2807345B1 (fr) * | 2000-04-07 | 2002-07-12 | Gilson Sa | Pipette de prelevement a demontage simplifie |
| FR2807340B1 (fr) | 2000-04-07 | 2002-12-06 | Gilson Sa | Pipette de prelevement d'echantillons liquides a mecanisme d'ejecteur de cone |
| FR2807341B1 (fr) | 2000-04-07 | 2002-07-12 | Gilson Sa | Pipette de prelevement d'echantillons liquides a ejecteur demontable |
| FR2807342B1 (fr) * | 2000-04-07 | 2002-07-12 | Gilson Sa | Pipette de prelevement a ejecteur de cone |
| EP1329262B1 (fr) | 2002-01-16 | 2009-08-19 | Brand Gmbh + Co Kg | Pipette et procédé pour sa production |
| FI20031683A0 (fi) * | 2003-11-19 | 2003-11-19 | Thermo Electron Oy | Pipetti jossa on kärjenpoistomekanismi |
| DE102004003433B4 (de) * | 2004-01-21 | 2006-03-23 | Eppendorf Ag | Pipettiervorrichtung mit einer Abwurfeinrichtung für Pipettenspitzen |
| US7641859B2 (en) * | 2004-02-11 | 2010-01-05 | Matrix Technologies Corporation | Pipette tip mounting and ejection assembly and associated pipette tip |
| DE602005016521D1 (de) * | 2005-02-04 | 2009-10-22 | Roche Diagnostics Gmbh | Pipette für Einwegspitzen unterschiedlicher Grösse |
| US20090071267A1 (en) * | 2007-09-17 | 2009-03-19 | Greg Mathus | Pipette tip ejection mechanism |
| PL233500B1 (pl) * | 2011-05-31 | 2019-10-31 | Pz Htl Spolka Akcyjna | Pipeta do używania z końcówką pipety |
| FI123652B (fi) * | 2011-10-20 | 2013-08-30 | Thermo Fisher Scientific Oy | Lukittava pipetti |
| US9815053B2 (en) * | 2013-01-15 | 2017-11-14 | Mettler-Toledo Rainin, LLC | Liquid end assembly for a multichannel air displacement pipette |
| CN103769254B (zh) * | 2014-01-10 | 2015-12-16 | 浙江大学 | 无损培养拾取转移装置及其方法 |
| USD963886S1 (en) * | 2021-03-12 | 2022-09-13 | Mettler-Toledo Rainin, LLC | Pipette body |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2287941A1 (fr) | 1974-10-15 | 1976-05-14 | Marteau D Autry Eric | Dispositif d'ejection de l'embout amovible d'une pipette |
| US4009611A (en) * | 1976-05-13 | 1977-03-01 | Oxford Laboratories Inc. | Hand-held micropipettor with improved pipette tip ejector |
| US4965050A (en) | 1989-01-18 | 1990-10-23 | Ballard Medical Products | Adaptation of pipetter |
| GB2249172A (en) | 1990-10-26 | 1992-04-29 | N Proizv Ob Biolog Priborostro | Multichannel pipette |
| US5320810A (en) * | 1992-05-13 | 1994-06-14 | Integrated Instrument Services, Inc. | Pipette with an axially stationary volume adjusting wheel |
| US5614153A (en) | 1995-05-26 | 1997-03-25 | Rainin Instrument Co., Inc. | Pipette tip ejector |
| DE19708151C2 (de) * | 1997-02-28 | 1999-05-27 | Eppendorf Geraetebau Netheler | Pipettiervorrichtung |
| FR2807342B1 (fr) * | 2000-04-07 | 2002-07-12 | Gilson Sa | Pipette de prelevement a ejecteur de cone |
| JP2004501745A (ja) * | 2000-06-26 | 2004-01-22 | ビスタラブ テクノロジーズ インク | 自動ピペット識別および先端部取外し |
| JP4148894B2 (ja) * | 2001-10-16 | 2008-09-10 | マトリックス・テクノロジイズ・コーポレーション | 手持ち式ピペット |
| EP1329262B1 (fr) * | 2002-01-16 | 2009-08-19 | Brand Gmbh + Co Kg | Pipette et procédé pour sa production |
-
1999
- 1999-05-18 AT AT99917735T patent/ATE249885T1/de active
- 1999-05-18 WO PCT/CH1999/000210 patent/WO2000069562A1/fr not_active Ceased
- 1999-05-18 AU AU35931/99A patent/AU3593199A/en not_active Abandoned
- 1999-05-18 EP EP99917735A patent/EP1185368B1/fr not_active Expired - Lifetime
- 1999-05-18 US US09/979,323 patent/US6833114B1/en not_active Expired - Lifetime
- 1999-05-18 ES ES99917735T patent/ES2207204T3/es not_active Expired - Lifetime
- 1999-05-18 DE DE69911474T patent/DE69911474T2/de not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| WO2000069562A1 (fr) | 2000-11-23 |
| ATE249885T1 (de) | 2003-10-15 |
| DE69911474D1 (de) | 2003-10-23 |
| EP1185368A1 (fr) | 2002-03-13 |
| AU3593199A (en) | 2000-12-05 |
| ES2207204T3 (es) | 2004-05-16 |
| DE69911474T2 (de) | 2004-07-22 |
| US6833114B1 (en) | 2004-12-21 |
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| PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
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| 17P | Request for examination filed |
Effective date: 20011129 |
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| 17Q | First examination report despatched |
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