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EP1047940A1 - Procede pour detecter l'origine d'animaux d'elevage ainsi que des produits qui en sont derives - Google Patents

Procede pour detecter l'origine d'animaux d'elevage ainsi que des produits qui en sont derives

Info

Publication number
EP1047940A1
EP1047940A1 EP98905259A EP98905259A EP1047940A1 EP 1047940 A1 EP1047940 A1 EP 1047940A1 EP 98905259 A EP98905259 A EP 98905259A EP 98905259 A EP98905259 A EP 98905259A EP 1047940 A1 EP1047940 A1 EP 1047940A1
Authority
EP
European Patent Office
Prior art keywords
immunogen
livestock
farm animal
enzyme
use according
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP98905259A
Other languages
German (de)
English (en)
Inventor
Wolf Bertling
Manfred Gareis
Martin Groschup
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
November AG Novus Medicatus Bertling Gesellschaft fuer Molekular Medizin
Original Assignee
November AG Novus Medicatus Bertling Gesellschaft fuer Molekular Medizin
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by November AG Novus Medicatus Bertling Gesellschaft fuer Molekular Medizin filed Critical November AG Novus Medicatus Bertling Gesellschaft fuer Molekular Medizin
Publication of EP1047940A1 publication Critical patent/EP1047940A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K11/00—Marking of animals

Definitions

  • the invention relates to a method and a use for verifying the origin of farm animals, in particular cattle, pigs and the like. the like , as well as products from it.
  • livestock such as cattle and pigs
  • an identifier for proof of origin which is stamped on a tag attached to the ear of the livestock (tattoo, transponder, etc. systems).
  • the farm animals are grouped according to their origin.
  • the meat of the slaughtered animals is then given a group-specific stamp.
  • the company has started to produce accompanying documents for every farm animal.
  • the proof of origin used according to the prior art is insufficient.
  • the object of the invention is to eliminate the disadvantages of the prior art.
  • forgery-proof proof is to be provided, with which the origin of both the live farm animal and products derived from it, such as meat, meat products and food of animal origin, can be reliably verified.
  • the method according to the invention has the advantage that the biological marking is inextricably linked to the farm animal and therefore cannot be exchanged or counterfeited. It is also possible to prove the origin of meat without a stamp of origin, even of meat used in the manufacture of meat products.
  • a protein and / or peptide is / are advantageously used as the immunogen (s), which / s neither as a vaccine nor via the food chain, nor via the environment in which usual livestock farming gets into the organism of the livestock and against which no antibodies are naturally formed in the livestock.
  • a protein and / or peptide coupled with a carrier is expediently used. - This enables a specific marking to be achieved in a simple manner, which is easily detectable and completely harmless for human consumption.
  • the immunogen is designed in such a way that the specific antibodies formed remain permanently in the organism of the livestock and can be detected by means of an enzyme-immunological or immunochemical detection method.
  • the marking can be carried out by a single application, which is expediently carried out at the earliest possible time after the birth of the farm animal.
  • the application can take place by the immunogen being applied to the farm animal by injection.
  • the immunogen can also pass through the mucous membrane, preferably the nasal mucosa, of the farm animal, e.g. with a nasal spray or by means of an implant.
  • a mixture of several different immunogens is advantageously applied.
  • a first immunogen can be used to identify the region of origin, such as Western Europe, Eastern Europe, South America, etc.
  • a second immunogen can be used to identify the country of origin
  • a third immunogen can be used to identify administrative units in the country concerned.
  • individual production and sales organizations eg producer rings, quality meat programs
  • the DNA coding for it can also be applied with a suitable expression vector. In this way, the immunogen is repeatedly re-expressed. In this case, repeated application of the immunogen to refresh the label is unnecessary.
  • Blood, milk or other body secretions can be used to detect the antibodies in the live farm animal, and blood and body secretions such as the dripping or squeezed juice of the meat can also be used in the slaughtered animal.
  • enzyme immunological or immunochemical detection is particularly simple if it is carried out using test sticks. - If the marking remains reserved for veterinarians under state supervision, the origin of e.g. products obtained from slaughtered livestock are practically checked and ascertained by everyone.
  • the immunogen can be a protein and / or peptide or a mixture of these which does not enter the organism of the livestock either as a vaccine or as a medicament, nor via the food chain in normal livestock farming and against which no antibodies are naturally formed in the livestock ; the protein and / or peptide can be coupled to a carrier.
  • an immunogen which is foreign to the organism to be labeled but which is harmless for biological labeling enables a surprisingly simple, inexpensive and forgery-proof proof of origin.
  • the DNA coding for it can also be applied.
  • the corresponding immunogen is formed in the body of the farm animal by expression. This ensures permanent marking.
  • the farm-animal can immunogens such as "keyhole limpet hemocyanin", "green fluorescent protein” are administered from Aequoria victoria, inactive snake toxins and Viruspro ⁇ teine.
  • immunogens such as "keyhole limpet hemocyanin", "green fluorescent protein” are administered from Aequoria victoria, inactive snake toxins and Viruspro ⁇ teine.
  • naturally occurring peptides or polypeptides such as hirudin, pheromonotropin or ranalexin can also be applied.
  • synthetically produced fragments of partial amino acid sequences of naturally occurring immunogens can also be used for biomarking.
  • artificial proteins and peptides, their amino acid sequence can also serve as immunogens does not correspond to any previously known substance, but is only assembled or manufactured for the purpose of biomarking
  • a DNA can also be applied which has a nucleotide sequence coding for the immunogen.
  • the DNA can contain partial sequences or the entire gene of "keyhole limpet hemocyanine". However, it can also be designed such that it describes only a short peptide, it being possible for several immunogens to be encoded on a single DNA. In the case of naturally occurring genes or sequences derived therefrom, both cDNA and genomic DNA with intron sequences can be applied.
  • the DNA can contain further sequences which are important for the expression and processing of the immunogen in the livestock organism.
  • promoter sequences and sequences which bring about transcription termination and polyadenylation of the mRNA and intracellular "trafficking" of the immunogen.
  • Suitable expression regulators are promoters which ensure a high transcription rate of the DNA, e.g. the "immediate early" promoter of the cytomegalovirus or the promoter of a livestock-specific "house-keeping" gene such as ⁇ -actin.
  • promoters are also suitable which specifically restrict the expression of the immunogen to predetermined body cells.
  • the polypeptide formed can be directed into the lumen of the endoplasmic reticulum by suitable "trafficking" signal sequences and directed into cellular distribution channels for secretion or expression and presentation on the cell surface.
  • the antibodies induced by the substances mentioned are detected by common detection methods such as ELISA, EIA and RIA, preferably using test sticks, e.g. be immersed in the dripping juice of the meat of the slaughtered farm animal.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Environmental Sciences (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Birds (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Biotechnology (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

L'invention concerne un procédé pour détecter l'origine d'animaux d'élevage, notamment de veaux, de porcs et analogue, comportant les étapes suivantes: (a) marquage biologique de l'animal d'élevage vivant par application d'au moins un antigène inoffensif pour l'animal et l'homme, et (b) identification de l'anticorps spécifique formé par l'application du ou des antigènes, au moyen d'un procédé de détection immuno-enzymatique ou immuno-chimique.
EP98905259A 1998-01-14 1998-01-14 Procede pour detecter l'origine d'animaux d'elevage ainsi que des produits qui en sont derives Withdrawn EP1047940A1 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/DE1998/000113 WO1999036775A1 (fr) 1998-01-14 1998-01-14 Procede pour detecter l'origine d'animaux d'elevage ainsi que des produits qui en sont derives

Publications (1)

Publication Number Publication Date
EP1047940A1 true EP1047940A1 (fr) 2000-11-02

Family

ID=6918612

Family Applications (1)

Application Number Title Priority Date Filing Date
EP98905259A Withdrawn EP1047940A1 (fr) 1998-01-14 1998-01-14 Procede pour detecter l'origine d'animaux d'elevage ainsi que des produits qui en sont derives

Country Status (4)

Country Link
EP (1) EP1047940A1 (fr)
JP (1) JP2002509251A (fr)
CA (1) CA2315023A1 (fr)
WO (1) WO1999036775A1 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19847118C2 (de) * 1998-10-13 2000-09-07 November Ag Molekulare Medizin Verfahren und Verwendung zur Markierung und Identifizierung applizierter Mittel
DE19944128A1 (de) 1999-09-15 2001-04-12 November Ag Molekulare Medizin Verfahren zum Markieren eines Tiers sowie davon stammenden Produkten
DE10011741A1 (de) * 2000-03-13 2001-09-20 Rausch Hans Verfahren und Verwendung zur biologischen Kennzeichnung von Tieren, Tierprodukten und tierischen Erzeugnissen
DE10112470B4 (de) * 2001-03-15 2008-02-28 Keller, Ruprecht, Priv.-Doz. Dr.Dr. Verfahren zur Proben-Identifizierung bei einem Säugetier sowie Kit zur Durchführung dieses Verfahrens
DE10130321A1 (de) * 2001-06-22 2003-01-09 Hans-Lorenz Werner Mittel, Verfahren und Verwendung von Testmarkersubstanzen zur Personenidentifizierung
DE10306789A1 (de) * 2003-02-18 2004-08-26 Responsif Gmbh Zusammensetzung zur Verabreichung an ein Lebewesen und Verfahren zur Markierung von Mitteln
WO2004078926A2 (fr) * 2003-02-28 2004-09-16 Board Of Trustees Operating Michigan State University Marqueurs immunologiques

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AR215459A1 (es) * 1976-04-02 1979-10-15 Brewer J Metodo para producir un agente terapeutico capaz de proporcionar una marca en un animal no humano para indicar la administracion del agente al animal y para proporcionar tambien un repositorio del agente a ser libeberado durante un espacio de tiempo
AU2985289A (en) * 1988-03-18 1989-09-21 Idetek Inc. Sulfa drug screening for domestic animals
GB2271848A (en) * 1992-10-23 1994-04-27 Richard James Guy Method of marking an animal or plant.
DE19643682C1 (de) * 1996-10-23 1998-01-15 Manfred Prof Dr Gareis Verfahren zum Herkunftsnachweis von Nutztieren sowie davon stammenden Produkten
GB2320960A (en) * 1997-01-03 1998-07-08 Gregory Scott Armitage Marking or tagging of livestock

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO9936775A1 *

Also Published As

Publication number Publication date
WO1999036775A1 (fr) 1999-07-22
CA2315023A1 (fr) 1999-07-22
JP2002509251A (ja) 2002-03-26

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