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EP0813361B1 - Solution de cryopreservation - Google Patents

Solution de cryopreservation Download PDF

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Publication number
EP0813361B1
EP0813361B1 EP96908717A EP96908717A EP0813361B1 EP 0813361 B1 EP0813361 B1 EP 0813361B1 EP 96908717 A EP96908717 A EP 96908717A EP 96908717 A EP96908717 A EP 96908717A EP 0813361 B1 EP0813361 B1 EP 0813361B1
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Prior art keywords
cells
cryopreservation solution
target cells
solution
cryopreservation
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German (de)
English (en)
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EP0813361A4 (fr
EP0813361A1 (fr
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Milo R. Polovina
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PROTIDE PHARMACEUTICALS, INC.
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Celox Laboratories Inc
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/14—Blood; Artificial blood
    • A61K35/19—Platelets; Megacaryocytes
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10—Preservation of living parts
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10—Preservation of living parts
    • A01N1/12—Chemical aspects of preservation
    • A01N1/122—Preservation or perfusion media
    • A01N1/125—Freeze protecting agents, e.g. cryoprotectants or osmolarity regulators
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/48—Reproductive organs
    • A61K35/51—Umbilical cord; Umbilical cord blood; Umbilical stem cells

Definitions

  • This invention relates to cryopreservation solutions. More specifically, this invention is directed to cryopreservation solutions which are particularly suited for use in cryopreserving the various types of cells circulating in blood or found in bone marrow such as hematopoietic stem and progenitor cells.
  • This invention also relates to methods for cryopreserving and recovering cryopreserved cells.
  • the morphologically recognizable and functionally capable cells circulating in blood include erythrocytes, neutrophilic, eosinophilic, and basophilic granulocytes, B-lymphocytes, T-lymphocytes, nonB-lymphocytes, nonT-lymphocytes, and platelets. These mature cells derive from and are replaced, on demand, by morphologically recognizable dividing precursor cells for the respective lineages such as erythroblasts for the erythrocyte series, myeloblasts, promyelocytes and myelocytes for the granulocyte series, and megakaryocytes for the platelets.
  • the precursor cells derive from more primitive cells that can simplistically be divided into two major subgroups: stem cells and progenitor cells.
  • stem cells have extensive self-renewal or self-maintenance capacity. Some of the stem cells differentiate upon need, but some stem cells or their daughter cells produce other stem cells to maintain the precious pool of these cells.
  • pluripotential stem cells are capable of differentiation into several sublines of progenitor cells with more limited self-renewal capacity or no self-renewal capacity. These progenitor cells ultimately give rise to the morphologically recognizable precursor cells.
  • the progenitor cells are capable of proliferating and differentiating along one, or more than one, of the myeloid differentiation pathways.
  • Stem and progenitor cells make up a very small percentage of the nucleated cells in the bone marrow, spleen, and blood. About ten times fewer of these cells are present in the spleen relative to the bone marrow, with even less present in the adult blood. As an example, approximately one in one thousand nucleated bone marrow cells is a progenitor cell; stem cells occur at a lower frequency.
  • mice could be protected against lethal irradiation by intravenous infusion of bone marrow (Lorenz, 20 E., et al., 1951, J. Natl. Cancer Inst. 12:197-201). Later research demonstrated that the protection resulted from colonization of recipient bone marrow by the infused cells (Lindsley, D.L., et al, 1955, Proc. Soc. Exp. Biol. Med. 90:512-515; Nowell, P.C., et al., 1956, Cancer Res. 16:258-261; Mitchison, N.A., 1956, Br. J. Exp.
  • Bone marrow has been used with increasing success to treat various fatal or crippling diseases, including certain types of anemias such as aplastic anemia (Thomas, E.D., et al., Feb. 5, 1972, The Lancet, pp. 284-289), Fanconi's anemia (Gluckman, E., et al., 1980, Brit J. Haematol. 45:557-564; Gluckman, E., et al., 1983, Brit J. Haematol. 54:431-440; Gluckman, E., et al., 1984, Seminars in Hematology:21 (1):20-26), immune deficiencies (Good, R.A., et al., 1985, Cellular Immunol.
  • aplastic anemia Thomas, E.D., et al., Feb. 5, 1972, The Lancet, pp. 284-289
  • Fanconi's anemia Gluckman, E., et al., 1980, Brit J.
  • cancers such as lymphomas or leukemias (Cahn, J.Y., et al., 1986, Brit. J. Haematol. 63:457-470; Blume, K.J. and Forman S.J., 1982, J. Cell. Physiol. Supp. 1:99-102; Cheever, M.A., et al., 1982, N. Engl. J. Med. 307(8):479-481), carcinomas (Blijham, G., et al., 1981, Eur. J.
  • Cancer 17(4):433-441 various solid tumors (Ekert, H., et al., 1982, Cancer 49:603-609; Spitzer, G., et al., 1980, Cancer 45:3075-3085), and genetic disorders of hematopoiesis.
  • Bone marrow transplantation has also recently been applied to the treatment of inherited storage diseases (Hobbs, J.R., 1981, Lancet 2:735-739), thalassemia major (Thomas, E.D., et al., 1982, Lancet 2:227-229), sickle cell disease (Johnson, F.J., et al., 1984, N. Engl. J. Med. 311:780-783), and osteopetrosis (Coccia, P.F., et al., 1980, N. Engl. J. Med. 302:701-708) (for general discussions, see Storb, R. and Thomas, E.D, 1983, Immunol. Rev.
  • Peripheral blood has also been investigated as a source of stem cells for hematopoietic reconstitution (Nothdurtt, W., et al., 1977, Scand. J. Haematol. 19:470-471; Sarpel, S.C., et al., 1979, Exp. Hematol. 7:113-120; Ragharachar, A., et al., 1983, J. Cell. Biochem. Suppl. 7A:78; Juttner, C.A., et al., 1985, Brit. J. Haematol. 61:739-745; Abrams, R.A., et al., 1983, J. Cell. Biochem. Suppl.
  • Freezing has long been used to preserve living cells, such as blood cells, after they have been removed or separated from a donating organism.
  • the cryopreservation and recovery of living cells has proven to be quite troublesome. Cells are subjected to relatively harsh conditions during both the freezing and thawing cycles involved in the cryopreservation of cells, resulting in a low survivability rate.
  • Freezing is destructive to most living cells. As the external medium freezes cells attempt to maintain equilibrium and lose water, thus increasing intracellular solute concentration, until intracellular freezing occurs at about minus 10-15° C. It is generally believed that both intracellular freezing and solution effects are responsible for cell injury. For example, it has been proposed that freezing destruction from extracellular ice is essentially a plasma membrane injury resulting from osmotic dehydration of the cell.
  • Cryoprotection by solute addition is thought to occur by two potential mechanisms: (i) intracellular; by reducing the amount of ice formed within the cell; and/or (ii) extracellular; by decreasing water flow out of the cell in response to a decreased vapor pressure caused by the formation of ice in the solute surrounding the cells.
  • optimal results for the freezing of hematopoietic stem and progenitor cells can be achieved with a cooling rate of about 1-2 °C per minute with a final storage temperature of about -70 to about -196°C with other blood cells generally within these same ranges.
  • DMSO dimethyl sulfoxide
  • DMSO is generally effective for these purposes, it is also known to be physiologically pernicious, particularly at higher concentrations, and thereby tends to irritate both those involved in the cryopreservation process and the patient into whom the cryopreserved cells are introduced with resultant hypertension, vomiting and nausea.
  • the pernicious nature of DMSO is also thought to result in a lower in vitro viability of the cryopreserved cells.
  • WO 91/03935 discloses a vitrification solution for cryopreservation of embryos comprising 25% glycerol + 25% propylene glycol in a rehydration medium that comprises ⁇ 1% Cl - , 0.1% glucose, 0.3% serum albumin, 0.125% PO 4 3- and antibiotics.
  • Valeri, Transfusion, Vol. 15, 195- 218 (1975) discloses the use of various concentrations of glycerol (20 - 40%) for the cryopreservation of red blood cells. After cryopreservation the red blood cells are thawed and washed with phosphate buffered 12% NaCl.
  • US 4,059,967 discloses an aqueous saline solution for cryopreservation of blood platelets comprising 4 - 7% glycerol, 2 - 6% glucose and 0.9% NaCl.
  • I have developed a simple, inexpensive, physiologically compatible, cryopreservation solution which includes the innocuous components of (i) glycerol, (ii) an alkali metal chloride salt, (iii) a monosaccharide, and (iv) serum albumin.
  • the major constituent of the cryopreservation solution is the cryopreserving agent glycerol (also referenced as glycerin).
  • the alkali metal chloride promotes cell viability through the freeze-thaw cycle.
  • the monosaccharide functions as a nutritional carbon source for the cells.
  • the serum albumin functions as a protein source which provides a coating around the membrane of the target cell so as to protect the membrane during the freeze-thaw cycle and storage of the frozen cells.
  • the serum albumin is also believed to function as a scavenger of oxygen free radicals which are known to adversely impact the viability of various target cells such as the erythrocyte, neutrophilic, eosinophilic, and basophilic granulocytes, B-lymphocytes, T-lymphocytes, nonB-lymphocytes, nonT-lymphocytes, and platelet cells mentioned previously.
  • the source of the serum albumin is preferably matched with the species of the intended subject into which the protected target cells will be introduced.
  • the cryopreservation solution may be provided in either concentrated or fully diluted form.
  • the concentrated form may be diluted to use strength by simply adding a diluent, such as water which has been suitably treated for injection, into the solution.
  • the diluent is preferably an physiologically balanced salt solution such as saline.
  • cryopreservation solution in the preservation and subsequent therapeutic use of target cells, such as whole blood, umbilical cord blood, bone marrow, granulocytes, neutrophils, platelets, and hematopoietic stem and progenitor cells including those identified as CD 34+ cells, includes the steps of (i) diluting the concentrated cryopreservation solution as necessary, (ii) introducing the target cells into the diluted solution, (iii) freezing the protected target cells, (iv) thawing the frozen solution to ambient conditions, and (v) introducing the thawed target cells into a subject in need of such target cells.
  • the thawed target cells and accompanying cryopreservation solution is preferably warmed to body temperature ( i.e. , about 37 °C) prior to introduction into the subject.
  • the physiological compatibility of the cryoprotection solution allows the thawed target cells to be introduced into the subject without separation of the target cells and the cryopreservation solution.
  • cryopreservation solution which consists essentially of (i) about 60 to about 80 wt.% glycerol, (ii) about 5 to about 10 wt.% alkali metal chloride salt, (iii) about 0.2 to about 1 wt.% biologically metabolizable monosaccharide, and (iv) about 20 to about 30 wt.% serum albumin.
  • wt% when used in connection with the constituents of the cryopreservation solution, is based upon the total weight of the concentrated cryopreservation solution unless otherwise stated.
  • Glycerol (C 3 H 8 O 3 ) is a polyol which is commercially available in U.S.P. and food grade from a number of suppliers including J.T. Baker.
  • the concentrated cryopreservation solution includes about 60 to about 80 wt% glycerol. Concentrations of less than about 60 wt% glycerol result in reduced cell viability while solutions containing greater than about 80 wt% glycerol result in a solution with an unacceptably high osmolality ( i.e. , greater than about 2,000 mOsm/kg).
  • Alkali metal chloride salts suitable for use in this invention include sodium chloride and potassium chloride.
  • the alkali metal chloride salt is believed to promote cell viability through the freeze-thaw cycle by adjusting the osmolality of the cryopreservation solution between about 500 and 2,000 mOsm/kg as measured with a Wescor 5500 Model Vapor Pressure Osmometer in accordance with the protocol provided in the instruction/service manual for the instrument.
  • the physiological osmolality of blood is generally within the range of about 260 to 320 mOsm/kg.
  • the optimum osmolality within this general range depends upon several factors including the particular target cells, the specific constitution of the cryopreservation solution and the freeze-thaw rate.
  • an osmolality about 700 to about 1,700 is generally desired for the cryopreservation of hematopoietic stem and progenitor cells.
  • the concentrated cryopreservation solution includes about 5 to about 10 wt% alkali metal chloride salt. Concentrations of less than about 5 wt% and greater than about 10 wt% alkali metal chloride salt result in reduced cell viability through the freeze-thaw cycle with concentrations of greater than 10 wt% also susceptible to damaging crystallization.
  • the monosaccharide such as glucose, functions as a nutritional carbon source for the cells. Availability of the monosaccharide promotes cell preservation through the freeze-thaw cycle and storage of the frozen cells by ensuring the presence of sufficient nutrients within the cell.
  • the serum albumin functions as a protein source which provides a coating around the membrane of the target cell so as to protect the membrane during the freeze-thaw cycle and storage of the frozen cells.
  • the serum albumin is also believed to function as a scavenger of oxygen free radicals which are known to adversely impact the viability of various target cells such as the erythrocyte, neutrophilic, eosinophilic, and basophilic granulocytes, B-lymphocytes, T-lymphocytes, nonB-lymphocytes, nonT-lymphocytes, and platelet cells mentioned previously.
  • the source of the serum albumin is preferably matched with the species of the intended subject into which the protected target cells will be introduced.
  • Effective concentrations can be attained by incorporation of about 0.2 to about 1 wt% monosaccharide in the concentrated cryopreservation solution.
  • a loading of less than about 0.2 wt% provides an insufficient concentration and reduces cell viability through the freeze-thaw cycle while concentrations greater than about 1 wt% do not provide a proportional increase in cell viability and adversely impact the concentrations of the other constituents.
  • the serum albumin functions as a protein source which provides a coating around the membrane of the target cell so as to protect the membrane during the freeze-thaw cycle and storage of the frozen cells.
  • Effective concentrations can be attained by incorporation of about 20 to about 30 wt% serum albumin.
  • a concentration of less than about 20 wt% serum albumin provides an insufficient concentration and results in reduced cell viability while concentrations greater than about 30 wt% do not provide a proportional increase in cell viability and tend to increase the osmolality of the solution above the desired upper limit of about 2,000 mOsm/kg.
  • the concentrated cryopreservation solution can be diluted to use concentration by the addition of a suitable diluent (e.g. , 0.9 wt% saline from ultrafiltered water, water for injection, and autologous plasma) at a ratio of from about 1 to 10 parts, typically 1 to 2 parts, diluent to 1 part concentrated cryopreservation solution.
  • a suitable diluent e.g. , 0.9 wt% saline from ultrafiltered water, water for injection, and autologous plasma
  • the diluted cryopreservation solution will consist essentially of (i) about 20 to about 40 wt% glycerol, (ii) about 1.5 to about 5 wt% alkali metal chloride salt, (iii) about 0.1 to about 0.5 wt% monosaccharide, and (iv) about 6 to about 15 wt% serum albumin, based upon the diluted cryopreservation solution.
  • the balance is diluent.
  • the cryopreservation solution should be formulated to provide a pH of between about 7 and 7.5.
  • Target cells may be conveniently cryopreserved using the diluted cryopreservation solution by simply (i) introducing the target cells into the diluted cryopreservation solution, and (ii) freezing or cryopreserving the protected target cells.
  • the volume of diluted cryopreservation solution which should be added to the target cells depends upon both the volumetric ratio of target cell containing solution to cryopreservation solution and the ratio of target cell to cryopreservation solution.
  • the volumetric ratio of target cell containing solution to cryopreservation solution should generally be between about 1:1 to 1:10 with the ratio of target cells to cryopreservation solution between about 1x10 5 cells/ml to about 1:1x10 9 cells/ml with a generally preferred ratio of between about 2x10 8 cells/ml to 3x10 8 cells/ml. Ratios of greater than those set forth above tend to result in a decrease in the concentration of viable cells due to insufficient cryopreservation solution, while ratios less than those set forth above simply result in an inefficient use of the cryopreservation solution and the need to introduce excessive cryopreservation solution into a subject in order to introduce effective amounts of the target cells.
  • Target cells are often available for preservation only in dilute form as a constituent in a biological fluid (e.g. , stem cells concentrated from peripheral blood commonly contain stem cells at a concentration of about 1x10 6 cells/ml to about 3x10 8 cells/ml with the balance comprising other blood components such as plasma and platelets).
  • These biologically diluted samples must generally be combined with the cryopreservation solution at a volumetric ratio of about 2:1 to 1:4 (depending of course upon a number of factors including the specific formulation of the diluted cryopreservation solution, the concentration of target cells in the biologically diluted sample, the type of target cells, etc.) to achieve the optimum ratio of target cells to cryopreservation solution.
  • the final, target cell containing solution should generally contain about 4 to 12 wt% glycerol and about 2 to 10 wt% albumin, with variations outside of this general range contemplated for certain applications.
  • Cryopreserved protected target cells must be thawed prior to introduction of the target cells into a subject. Thanks to the physiological compatibility of the cryoprotection solution, the thawed target cells may to be introduced into the subject without separating the target cells and the cryopreservation solution. The thawed target cells may be maintained for several hours at room temperature without an appreciable loss in cell viability.
  • the thawed solution may be slowly diluted with saline or other appropriate ionic solution to so as to reduce the osmolality of the solution from the desired cryopreservation range of 500 to 2,000 mOsm/kg down to the physiological range of 260 to 320 mOsm/kg so as to reduce shock to the cell walls caused by sudden changes in osmolality.
  • a controlled slow cooling rate is can often be effective for achieving optimal cell viability. It is generally believed that different cell types have different optimal cooling rates (See, e.g. Rowe, A.W. and Rinfret, A.P., 1962, Blood 20:636; RoWe, A.W., 1966, Cryo Biology 3(1):12-18; Lewis, J.P., et al., 1967, transfusion 7(1):17-32; and Mazur, P, 1970 Science 168:939-949 for effects of cooling velocity on survival of marrow-stem cells and on their transplantation potential).
  • the cryopreservation solution can be maintained at room temperature prior to use and the blended target cells held at room temperature for as long as thirty minutes before freezing is commenced without an appreciable loss in cell viability.
  • This is in contrast to the toxic cryoprotectant DMSO which must be cooled to about 4°C prior to the addition of target cells in order to expedite the freezing process and requires that the freezing process commence immediately after the DMSO is added to the cells in order to minimize cell death induced by the presence of DMSO.
  • Controlled freezing can be carried out by use of a programmable freezing device.
  • Programmable freezing apparatuses allow determination of optimal cooling rates and facilitate standard reproducible cooling.
  • the container holding the cells must be stable at cryogenic temperatures and allow for rapid heat transfer for effective control of both freezing and thawing.
  • Sealed plastic vials e.g ., Nunc and Wheaton cryules
  • glass ampules can be used for multiple small amounts (1 to 2 ml), while larger volumes of 100 to 200 ml can be frozen in polyolefin bags, such as those available from Fenwal, held between metal plates.
  • samples can then be transferred to a long-term cryogenic storage vessel.
  • samples can be cryogenically stored in liquid nitrogen (-196 °C) or liquid nitrogen vapor (-105 °C). Such storage is greatly facilitated by the availability of highly efficient liquid nitrogen refrigerators.
  • the frozen cells are preferably thawed quickly and maintained at about 37 °C until used. Since the cryoprotective solution is physiologically compatible, it need not be removed prior to introduction into the subject.
  • Cryopreserved and thawed umbilical cord cells, platelets, and hematopoietic stem and progenitor cells can be used therapeutically for reconstitution of the hematopoietic system in a suitable patient.
  • the cells can be introduced by any method known in the art with systemic infusion generally preferred.
  • Reconstitution of the hematopoietic system can be therapeutically valuable for a large number of diseases and disorders.
  • the infusion of cyropreserved hematopoietic stem and progenitor cells for hematopoietic reconstitution can be beneficially used in the treatment of those diseases which are presently known to be curable by autogeneic bone marrow transplantation.
  • disorders that can be treated by infusion of stem cells include, but are not limited to, five broad categories.
  • First are diseases resulting from a failure or dysfunction of normal blood cell production and maturation (i.e., aplastic anemia and hypoproliferative stem cell disorders).
  • the second group includes neoplastic malignant diseases in the hematopoietic organs (e.g., leukemia and lymphomas).
  • the third group of disorders comprises those patients with a broad spectrum of malignant solid tumors of non-hematopoietic origin. Stem cell infusion in these patients serves as a bone marrow rescue procedure, which is provided to a patient following otherwise lethal chemotherapy or irradiation of the malignant tumor.
  • the fourth group of diseases consists of autoimmune conditions, where the stem cells serve as a source of replacement of an abnormal immune system.
  • the fifth group of diseases comprises a number of genetic disorders which can be corrected by infusion of hematopoietic stem cells, preferably syngeneic, which prior to transplantation have undergone gene therapy.

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Claims (18)

  1. Solution de cryoconservation concentrée consistant essentiellement en :
    (a) 60 à 80 % en pds de glycérol ;
    (b) 5 à 10 % en pds d'un sel chlorure de métal alcalin ;
    (c) 0,2 à 1 % en pds d'un monosaccharide biologiquement métabolisable ; et
    (d) 20 à 30 % pds de sérumalbumine.
  2. Solution de cryoconservation de la revendication 1, comprenant en outre un diluant.
  3. Solution de cryoconservation de la revendication 1 ou 2, dans laquelle le pH de la solution est compris entre environ 7 et 7,5.
  4. Solution de cryoconservation de la revendication 2, dans laquelle la solution consiste essentiellement en (a) environ 20 à environ 40 % en pds de glycérol, (b) environ 1,5 à environ 5 % en pds d'un sel chlorure de métal alcalin ; (c) environ 0,1 à environ 0,5 % en pds d'un monosaccharide, et (d) environ 6 à environ 15 % en pds de sérumalbumine et (e) le complément en diluant.
  5. Solution de cryoconservation de l'une quelconque des revendications 1 à 4, dans laquelle le sel chlorure de métal alcalin est le chlorure de sodium.
  6. Solution de cryoconservation de l'une quelconque des revendications 1 à 5, dans laquelle le monosaccharide est le glucose.
  7. Solution de cryoconservation de l'une quelconque des revendications 1 à 6, dans laquelle la sérumalbumine est la sérumalbumine humaine.
  8. Procédé de cryoconservation de cellules, comprenant (i) l'introduction de cellules cibles dans la solution de cryoconservation de la revendication 2, et (ii) la cryoconservation des cellules cibles protégées.
  9. Procédé de la revendication 8, dans laquelle les cellules cibles sont des cellules précurseurs ou souches hématopoïétiques, des cellules de plaquettes sanguines, des cellules identifiées comme étant des cellules CD34+ ou des cellules cibles obtenues à partir de sang de cordon ombilical.
  10. Procédé de cryoconservation de cellules, comprenant (i) l'introduction des cellules cibles dans la solution de cryoconservation de la revendication 4, le monosaccharide étant du glucose et (ii) la cryoconservation des cellules cibles protégées.
  11. Procédé de la revendication 10, dans laquelle les cellules cibles protégées sont refroidies à une vitesse de 1-2 °C par minute jusqu'à une température comprise entre environ -70 et environ -196 °C.
  12. Procédé de cryoconservation et de récupération des cellules viables, comprenant (i) l'introduction de cellules cibles dans la solution de cryoconservation de la revendication 2, (ii) la cryoconservation des cellules cibles protégées, et (iii) la décongélation des cellules cibles protégées.
  13. Procédé de la revendication 12, dans laquelle les cellules cibles sont des cellules précurseurs ou souches ou hématopoïétiques.
  14. Méthode de la revendication 8, 10 ou 12, dans laquelle le rapport volumétrique de la solution contenant' les cellules cibles à la solution de cryoconservation est compris entre environ 1/1 et 1/10 et le rapport des cellules cibles à la solution de cryoconservation est compris entre environ 1 x 105 cellules/ml et environ 1 x 109 cellules/ml.
  15. Utilisation des cellules cibles protégées décongelées obtenues comme défini dans la revendication 12 sans séparation des cellules cibles et de la solution de cryoconservation pour la préparation d'une composition pharmaceutique destinée à la reconstitution du système hématopoïétique ou du système immunitaire.
  16. Utilisation de la revendication 15, dans laquelle le rapport volumétrique de la solution contenant les cellules cibles à la solution de cryoconservation est compris entre environ 1/1 et 1/10 et le rapport des cellules cibles à la solution de cryoconservation est compris entre environ 1 x 105 cellules/ml et environ 1 x 109 cellules/ml.
  17. Utilisation de la revendication 15 ou 16, dans laquelle les cellules cibles sont des cellules précurseurs ou souches hématopoïétiques, des cellules de plaquettes sanguines, des cellules identifiées comme étant des cellules CD34+ ou des cellules cibles obtenues à partir de sang de cordon ombilical.
  18. Utilisation de l'une quelconque des revendications 15 à 17, dans laquelle la composition pharmaceutique est destinée à la reconstitution du système hématopoïétique d'un humain.
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ES2210352T3 (es) 2004-07-01
EP0813361A4 (fr) 2000-01-05
EP0813361A1 (fr) 1997-12-29
NZ304628A (en) 1998-09-24
MX9706843A (es) 1998-06-30
JPH10511402A (ja) 1998-11-04
AU693339B2 (en) 1998-06-25
US5759764A (en) 1998-06-02
ATE253818T1 (de) 2003-11-15
CA2214280C (fr) 2001-01-02
NO974108D0 (no) 1997-09-05
BR9607147A (pt) 1997-11-25
RU2146088C1 (ru) 2000-03-10
DE69630679T2 (de) 2004-10-21
DE69630679D1 (de) 2003-12-18
WO1996027287A1 (fr) 1996-09-12
US5580714A (en) 1996-12-03
JP3165863B2 (ja) 2001-05-14
CA2214280A1 (fr) 1996-09-12
AU5186996A (en) 1996-09-23
NO974108L (no) 1997-11-06

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