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DK4110957T3 - Fremgangsmåder til analyse af cellefrie nukleinsyrer og anvendelser heraf - Google Patents

Fremgangsmåder til analyse af cellefrie nukleinsyrer og anvendelser heraf

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Publication number
DK4110957T3
DK4110957T3 DK21759938.0T DK21759938T DK4110957T3 DK 4110957 T3 DK4110957 T3 DK 4110957T3 DK 21759938 T DK21759938 T DK 21759938T DK 4110957 T3 DK4110957 T3 DK 4110957T3
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DK
Denmark
Prior art keywords
analysis
applications
cell
methods
nucleic acids
Prior art date
Application number
DK21759938.0T
Other languages
English (en)
Inventor
Maximilian Diehn
Arash Ash Alizadeh
Jacob J Chabon
David M Kurtz
Mohammad Shahrokh Esfahani
Original Assignee
Univ Leland Stanford Junior
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Filing date
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Application filed by Univ Leland Stanford Junior filed Critical Univ Leland Stanford Junior
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Publication of DK4110957T3 publication Critical patent/DK4110957T3/da

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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06Biochemical methods, e.g. using enzymes or whole viable microorganisms
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1082Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855Ligating adaptors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B20/00ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B40/00ICT specially adapted for biostatistics; ICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
    • G16B40/20Supervised data analysis
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16HHEALTHCARE INFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR THE HANDLING OR PROCESSING OF MEDICAL OR HEALTHCARE DATA
    • G16H50/00ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics
    • G16H50/20ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for computer-aided diagnosis, e.g. based on medical expert systems
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16HHEALTHCARE INFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR THE HANDLING OR PROCESSING OF MEDICAL OR HEALTHCARE DATA
    • G16H50/00ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics
    • G16H50/30ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for calculating health indices; for individual health risk assessment
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/156Polymorphic or mutational markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Medical Informatics (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Pathology (AREA)
  • Data Mining & Analysis (AREA)
  • Public Health (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Databases & Information Systems (AREA)
  • Evolutionary Biology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Theoretical Computer Science (AREA)
  • Epidemiology (AREA)
  • Oncology (AREA)
  • Hospice & Palliative Care (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Artificial Intelligence (AREA)
  • Primary Health Care (AREA)
  • Software Systems (AREA)
  • Evolutionary Computation (AREA)
  • Bioethics (AREA)
DK21759938.0T 2020-02-24 2021-02-24 Fremgangsmåder til analyse af cellefrie nukleinsyrer og anvendelser heraf DK4110957T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202062980972P 2020-02-24 2020-02-24
PCT/US2021/019478 WO2021173722A2 (en) 2020-02-24 2021-02-24 Methods of analyzing cell free nucleic acids and applications thereof

Publications (1)

Publication Number Publication Date
DK4110957T3 true DK4110957T3 (da) 2025-10-27

Family

ID=77490525

Family Applications (1)

Application Number Title Priority Date Filing Date
DK21759938.0T DK4110957T3 (da) 2020-02-24 2021-02-24 Fremgangsmåder til analyse af cellefrie nukleinsyrer og anvendelser heraf

Country Status (9)

Country Link
US (2) US20230242980A1 (da)
EP (3) EP4110957B1 (da)
KR (2) KR20220157976A (da)
CN (2) CN116113436A (da)
AU (2) AU2021227229A1 (da)
CA (2) CA3172670A1 (da)
DK (1) DK4110957T3 (da)
ES (1) ES3040741T3 (da)
WO (2) WO2021173722A2 (da)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11728007B2 (en) * 2017-11-30 2023-08-15 Grail, Llc Methods and systems for analyzing nucleic acid sequences using mappability analysis and de novo sequence assembly
GB2627085B (en) 2019-11-06 2024-11-13 Univ Leland Stanford Junior Methods and systems for analysing nucleic acid molecules
US11783912B2 (en) 2021-05-05 2023-10-10 The Board Of Trustees Of The Leland Stanford Junior University Methods and systems for analyzing nucleic acid molecules
WO2023039580A2 (en) * 2021-09-13 2023-03-16 Regeneron Pharmaceuticals, Inc. Methods of treating clonal hematopoiesis of indeterminate potential (chip) with lymphocyte antigen 75 (ly75), cluster of differentiation 164 (cd164), or poly(adp-ribose) polymerase 1 (parp1) inhibitors
CA3236814A1 (en) * 2021-11-17 2023-05-25 Maximilian Diehn Systems and methods for gene expression and tissue of origin inference from cell-free dna
WO2024097217A1 (en) * 2022-11-01 2024-05-10 Petdx, Inc. Detection of non-cancer somatic mutations
WO2025096466A2 (en) * 2023-10-30 2025-05-08 Myriad Women's Health, Inc. Methods for improving minimal residual disease assays

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA186569A (en) 1918-04-02 1918-09-17 Henry Arthur Dygert Surgical appliance
CA188298A (en) 1918-04-08 1919-01-14 William H. Bot Grain separator
US6171856B1 (en) * 1997-07-30 2001-01-09 Board Of Regents, The University Of Texas System Methods and compositions relating to no-mediated cytotoxicity
CN1629633A (zh) * 2000-03-16 2005-06-22 有限会社环境技术研究所 利用dna损伤指标的天然与人工化学物质的简易生物学的评价法
AU2001255518A1 (en) * 2000-06-07 2001-12-17 Baylor College Of Medicine Compositions and methods for array-based nucleic acid hybridization
DE10032165A1 (de) * 2000-07-01 2002-01-10 Beiersdorf Ag Verwendung von physiologisch verträglichen Sulfinsäuren als Antioxidans oder Radikalfänger in kosmetischen oder dermatologischen Zubereitungen
NO2398912T3 (da) * 2009-02-18 2018-02-10
ES2925014T3 (es) * 2014-09-12 2022-10-13 Univ Leland Stanford Junior Identificación y uso de ácidos nucleicos circulantes
US11078521B2 (en) * 2016-10-24 2021-08-03 Biomatrica, Inc. Stabilization of nucleic acids on paper
CN118638898A (zh) * 2017-03-23 2024-09-13 华盛顿大学 用于靶向核酸序列富集的方法及在错误纠正的核酸测序中的应用
US11584958B2 (en) * 2017-03-31 2023-02-21 Grail, Llc Library preparation and use thereof for sequencing based error correction and/or variant identification
GB2578038B (en) * 2017-06-16 2022-11-23 Life Technologies Corp Control nucleic acids, and compositions, kits, and uses thereof
US11447818B2 (en) 2017-09-15 2022-09-20 Illumina, Inc. Universal short adapters with variable length non-random unique molecular identifiers

Also Published As

Publication number Publication date
KR20220145891A (ko) 2022-10-31
CN115443341A (zh) 2022-12-06
EP4110397A4 (en) 2024-05-01
AU2021227229A1 (en) 2022-10-13
WO2021173724A1 (en) 2021-09-02
EP4110957A2 (en) 2023-01-04
EP4110957A4 (en) 2024-03-06
ES3040741T3 (en) 2025-11-04
WO2021173722A2 (en) 2021-09-02
CN116113436A (zh) 2023-05-12
US20250263789A1 (en) 2025-08-21
EP4660324A2 (en) 2025-12-10
KR20220157976A (ko) 2022-11-29
EP4110957B1 (en) 2025-08-27
CA3172670A1 (en) 2021-09-02
AU2021225854A1 (en) 2022-10-13
WO2021173722A3 (en) 2021-10-07
EP4110397A1 (en) 2023-01-04
US20230242980A1 (en) 2023-08-03
CA3172675A1 (en) 2021-09-02

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